Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Lithospermum erythrorhizon
Species name
Lithospermum erythrorhizon is a species of eudicot in the family Boraginaceae (borage family).
Species info
Gene sequence info
Lithospermum_erythrorhizon.COMT.fa
Download
>rna-gnl|WGS:JADFUC|Leryth_016634-t1
MGSAIDIKTNHIEQEEQHCAYAIQLLTSASLPFVLNAAIELQVFEIIAKCGPNSQISAAEIASQMPTKNPEAASMLDRMLRLLACYSVLTCSVIEKDENDDDDCLAGRVYGLAPVGKFFVKNKDGASMGPLCALLQDKLFIQSWFELKDSVLEGGIPFNRAHGTHAFDYPSKHTGFNKLFNNAMLNHTSLIMGSILDSYKGFDELKTLVDVGGGLGINLKLITSKHPTIKGINFDLLHVVEKAPSYPGVDHVGGDMFESVPQGDAIFMKWILHDWSDDHCLKLLKNCYKSIPEDGKVIVVESIYLPKPDSSASAISSSHMDVLMMTQSPGGKERSEKEFIALAKGAGFKGVRLESFVCNFWVMEFYK
>rna-gnl|WGS:JADFUC|Leryth_006467-t1
MSQNTSDVEQEEQHCAHAMQLVTSVAVPFVLNAVIELQVFEILAKAGANVGLSAKEIASHQKIPLKFALVVEKDGMSDDYRQALHTKLIRFLKVAFHLIELMVPMGLIMPEMILGIEHVGGDMFMSVPQGCYFHRGFLAGKLISKSQLIYYMWILNDWSDENCIKLLNNCNKSLPNDGKVIVADHIYPINLDSSASVKYTSEFDVLMMTQAPGGEVRSEEFLALAKGGGFKGI
>rna-gnl|WGS:JADFUC|Leryth_000218-t1
MGSTPNNNYTNTGVDEDEAGMFAMQLASASVLPMVLKTAIELDLLEIIAKAGANAYLSPSEIATQLPTQSNPEAPIMLDRILRLLACYKVLNCTLKELDDGSVQRLYGLAPVCKYLTKNEDGVCLAPLLLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFDYHGTDPRFNKVFNLGMSNHSTITMKKILENYKGFEGLNTVVDVGGGTGATLNMIVSKYPLIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKVCYQALPENGKMLIAECILPEEPDNKLTTQNVVHIDVIMLAHNPGGKERTEKEFQALAKASGFKEFRKTRFVDRSYLAVFYSIVLRLEEKDNARKAKSAKIRGVRCESHSVELETTSTSGKVGDDKKVGLSSPPYPGGVGPFTGRDPNVKKPGWLRQKAPQGEKYEEVKEQLSQLKLNTVCEEAQCPNIGECWNGGGDGISTATIMLLGDTCTRGCRFCAVKTSRNPAPPDPMEPFNTAKAIASWGVDRDDIPDGGSGHFAETVRSMKNLNPDIMIECLTSDFRGDLNAVSTLVHSGLDVFAHNIETVRRLQRIVRDPRAGYEQSLSVLRHAKLDKEGMITKTSIMLGLGESDDEIKEAMADLRAIDVDILTLGQYLQPTPLHLTVKEYVTPEKFAFWKEYGESFGFRYVASGPLVRSSYRAGELFVKTMVKESAKATSKHNTKG
>rna-gnl|WGS:JADFUC|Leryth_021206-t1
MGSVQHVFPIRKEEINFLQAMQLPGNAVLCLVLKAVIELDLFEIMAKAGPNAQISASEIVTSLPTSNPEAPLMLDRMLSFLASYSIFKCTVVTDKDGVDKKLYSLTPICSNYIRNEDGFAIGPLLLLQFEKVFMQAWYQLKDSVLEGGTPFNREYGMHAFDYPSTDSRVNDVLNKGLQNLTGVTMKIILEVYKGFDGMEELIDVGGGYGASLAWIVSKYPKIRGINYDLPHVIKDAPVLQGVEHVEGNMFESVPRGKTIFMKLILHDWDDDHCIRLLKNCFEALPNCGKVILVESILPESPESDCVSNISYYYDMMMMAYCHGGKERTLKQLEALAKAAGFSAIKPITQAYFYWVTEIYKT
>rna-gnl|WGS:JADFUC|Leryth_021210-t1
MGSIQVEFPIRNEENNFLQAMQLPGSAVLCLVLKAVIELDLFEIMAKAGPNAQISASEIVTSLPTSNPEASLVLDRMLRFLASYSILKCSVVVDEEGNDKRLYSLTPICSNYIRNEDGFAIAPYLLAQFDKVFMQAWYQLKDSILEGGTPFNREYGMHAFEYPGKDSRFNDVFNKGLQNLTGVTMKIILEVYKGFDGIEEVIDVGGGYGASLAWIVSKYPKIRGINFDLPHVIKDAPAIQGVEHVEGNMFESVPKVKTIFIKLILHDWDDDHCIRLLKNCFEALPNCGKVILVESILPESPQSDCVSNISFYFDIMMMTYNPGGRERTLKQFEALAKSAGFSAIKPIRQAYFYWVIEVYKN
MGSAIDIKTNHIEQEEQHCAYAIQLLTSASLPFVLNAAIELQVFEIIAKCGPNSQISAAEIASQMPTKNPEAASMLDRMLRLLACYSVLTCSVIEKDENDDDDCLAGRVYGLAPVGKFFVKNKDGASMGPLCALLQDKLFIQSWFELKDSVLEGGIPFNRAHGTHAFDYPSKHTGFNKLFNNAMLNHTSLIMGSILDSYKGFDELKTLVDVGGGLGINLKLITSKHPTIKGINFDLLHVVEKAPSYPGVDHVGGDMFESVPQGDAIFMKWILHDWSDDHCLKLLKNCYKSIPEDGKVIVVESIYLPKPDSSASAISSSHMDVLMMTQSPGGKERSEKEFIALAKGAGFKGVRLESFVCNFWVMEFYK
>rna-gnl|WGS:JADFUC|Leryth_006467-t1
MSQNTSDVEQEEQHCAHAMQLVTSVAVPFVLNAVIELQVFEILAKAGANVGLSAKEIASHQKIPLKFALVVEKDGMSDDYRQALHTKLIRFLKVAFHLIELMVPMGLIMPEMILGIEHVGGDMFMSVPQGCYFHRGFLAGKLISKSQLIYYMWILNDWSDENCIKLLNNCNKSLPNDGKVIVADHIYPINLDSSASVKYTSEFDVLMMTQAPGGEVRSEEFLALAKGGGFKGI
>rna-gnl|WGS:JADFUC|Leryth_000218-t1
MGSTPNNNYTNTGVDEDEAGMFAMQLASASVLPMVLKTAIELDLLEIIAKAGANAYLSPSEIATQLPTQSNPEAPIMLDRILRLLACYKVLNCTLKELDDGSVQRLYGLAPVCKYLTKNEDGVCLAPLLLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFDYHGTDPRFNKVFNLGMSNHSTITMKKILENYKGFEGLNTVVDVGGGTGATLNMIVSKYPLIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKVCYQALPENGKMLIAECILPEEPDNKLTTQNVVHIDVIMLAHNPGGKERTEKEFQALAKASGFKEFRKTRFVDRSYLAVFYSIVLRLEEKDNARKAKSAKIRGVRCESHSVELETTSTSGKVGDDKKVGLSSPPYPGGVGPFTGRDPNVKKPGWLRQKAPQGEKYEEVKEQLSQLKLNTVCEEAQCPNIGECWNGGGDGISTATIMLLGDTCTRGCRFCAVKTSRNPAPPDPMEPFNTAKAIASWGVDRDDIPDGGSGHFAETVRSMKNLNPDIMIECLTSDFRGDLNAVSTLVHSGLDVFAHNIETVRRLQRIVRDPRAGYEQSLSVLRHAKLDKEGMITKTSIMLGLGESDDEIKEAMADLRAIDVDILTLGQYLQPTPLHLTVKEYVTPEKFAFWKEYGESFGFRYVASGPLVRSSYRAGELFVKTMVKESAKATSKHNTKG
>rna-gnl|WGS:JADFUC|Leryth_021206-t1
MGSVQHVFPIRKEEINFLQAMQLPGNAVLCLVLKAVIELDLFEIMAKAGPNAQISASEIVTSLPTSNPEAPLMLDRMLSFLASYSIFKCTVVTDKDGVDKKLYSLTPICSNYIRNEDGFAIGPLLLLQFEKVFMQAWYQLKDSVLEGGTPFNREYGMHAFDYPSTDSRVNDVLNKGLQNLTGVTMKIILEVYKGFDGMEELIDVGGGYGASLAWIVSKYPKIRGINYDLPHVIKDAPVLQGVEHVEGNMFESVPRGKTIFMKLILHDWDDDHCIRLLKNCFEALPNCGKVILVESILPESPESDCVSNISYYYDMMMMAYCHGGKERTLKQLEALAKAAGFSAIKPITQAYFYWVTEIYKT
>rna-gnl|WGS:JADFUC|Leryth_021210-t1
MGSIQVEFPIRNEENNFLQAMQLPGSAVLCLVLKAVIELDLFEIMAKAGPNAQISASEIVTSLPTSNPEASLVLDRMLRFLASYSILKCSVVVDEEGNDKRLYSLTPICSNYIRNEDGFAIAPYLLAQFDKVFMQAWYQLKDSILEGGTPFNREYGMHAFEYPGKDSRFNDVFNKGLQNLTGVTMKIILEVYKGFDGIEEVIDVGGGYGASLAWIVSKYPKIRGINFDLPHVIKDAPAIQGVEHVEGNMFESVPKVKTIFIKLILHDWDDDHCIRLLKNCFEALPNCGKVILVESILPESPQSDCVSNISFYFDIMMMTYNPGGRERTLKQFEALAKSAGFSAIKPIRQAYFYWVIEVYKN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University