Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Leavenworthia alabamica
Species name
Leavenworthia alabamica is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Leavenworthia_alabamica.FLS.fa
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>LA_scaffold220_1.mRNA1
MEKPMFKTVQEVVEAGEELPERYIHTRTGDGNGEPLNASVPEMDIPAIDLSLLFSSSEAGQEELSKLHVALSKWGVIQVMNHGITKEFLDKIYKLTKQFFMLPTEEKHKYAREIDNIQGYGNDMILSDDQVLDWIDRLLLTTYPEDQRQLKFWPQDPIEFKDTLHEYTMKTRPLIEQFFKAMARALEVEENSFLEMNGIVMETRFNSYPPCPRPDIIIGVKPHSDGSAFTLLLPDKDVEGLQYLKDGKWYKPPVVPDTILINVGDLMEIALRVHKVWETIDPGTDDVEKNDLAMALLFQSIPETMTLQLGELESSKAVWDAIKARHVGAERVKEARLQTLMADFERMKMKDTDTIDDFVGKLSELSSKSAALGEVIEESKLVKKFLNSLPRRKFIHIIAALEQVLDLKTTSFEDIIGRLKAYEERIYDDENQQDDQGKLMYVDSQSYGEGRGGRGRGSRGYVRGRGRGHFGNEGIKQENDKSKLLCWRCDKAGHFAYKCPDRLLKLQETMENEDDDTQEADNLMMHEVVYLNERN
>LA_scaffold580_22.mRNA1
MELPVVDLSRYLDYSGDQLGTDLLDSCREVSRILRETGALLIKDPRCSAEDNDRFIDMMETFFQMPDDFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPKEYKPHIPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPEGFPEWKEVMDLWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLNCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLKNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKDAIKLAKEQNRSLWRVSSTLFAHIASDSELKPLGHFAESSLASNYPAIPAGEYVEQELSVINLKGKKGFS
>LA_scaffold833_16.mRNA1
MAVPVIDLSSTNEKLLVQEVMKASEEWGIFQIVNHGVPVELMQRLQSTGKQFFELPEAEKELVAKDGDFEGFKRNYLGGIKNWDEHLFHRLSPPSILNYKYWPKNPPQYREVTEEYTKEMQRLTDKFLGFLSEGLGLPRLTLTERIGGDTAEYVLRINFYPPTSHTDLTIGAAPHTDMGVITFLIPNEVPGLQAFKDEQWLDLNYIDTAVIVIIGDQLQRMTNGRFKNVYHRAKTDQEKLRISWPVFIAPRADMVVGPLPELTGKSNPPKFETLVYNDYIDQKIKGWALEDLPVYGNPNDKKNT
>LA_scaffold1381_7.mRNA1
MEKPKFKTVQEVVAAGKGLPERYLFTPTGHGEGEPLNASVPDIALPVMNHGISEAYINKVYELTKQFFALPTEEKEKYAREIGKIQGYGNDLIKFSIGSTVYFSLLTLKINVKLNYGLKSLSDSFDG
>LA_scaffold1588_27.mRNA1
MEVERVQDIASSSLLNEAIPLEFIRSEKDQPATTTFRGPTPAIPVVDLSDPDKESVKRAVVKASEEWGLFQVVNHGIPTELIRKLQDVGKKFFELPSSEKESVAKPEDSKDIEGYGTKISKDLEGKKAWVDHLFHRIWPSSCVNYSFWPKNPPEYREVNEEYAIHVKKLSETLLEFLSEGLGLKREALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGLTLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGKYKNVLHRTTVDKEKTRMSWPVFLEPYREMIVGPLPELTGDNNPPKFKPLAFKDYSYRKLNKLPLD
MEKPMFKTVQEVVEAGEELPERYIHTRTGDGNGEPLNASVPEMDIPAIDLSLLFSSSEAGQEELSKLHVALSKWGVIQVMNHGITKEFLDKIYKLTKQFFMLPTEEKHKYAREIDNIQGYGNDMILSDDQVLDWIDRLLLTTYPEDQRQLKFWPQDPIEFKDTLHEYTMKTRPLIEQFFKAMARALEVEENSFLEMNGIVMETRFNSYPPCPRPDIIIGVKPHSDGSAFTLLLPDKDVEGLQYLKDGKWYKPPVVPDTILINVGDLMEIALRVHKVWETIDPGTDDVEKNDLAMALLFQSIPETMTLQLGELESSKAVWDAIKARHVGAERVKEARLQTLMADFERMKMKDTDTIDDFVGKLSELSSKSAALGEVIEESKLVKKFLNSLPRRKFIHIIAALEQVLDLKTTSFEDIIGRLKAYEERIYDDENQQDDQGKLMYVDSQSYGEGRGGRGRGSRGYVRGRGRGHFGNEGIKQENDKSKLLCWRCDKAGHFAYKCPDRLLKLQETMENEDDDTQEADNLMMHEVVYLNERN
>LA_scaffold580_22.mRNA1
MELPVVDLSRYLDYSGDQLGTDLLDSCREVSRILRETGALLIKDPRCSAEDNDRFIDMMETFFQMPDDFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPKEYKPHIPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPEGFPEWKEVMDLWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLNCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLKNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKDAIKLAKEQNRSLWRVSSTLFAHIASDSELKPLGHFAESSLASNYPAIPAGEYVEQELSVINLKGKKGFS
>LA_scaffold833_16.mRNA1
MAVPVIDLSSTNEKLLVQEVMKASEEWGIFQIVNHGVPVELMQRLQSTGKQFFELPEAEKELVAKDGDFEGFKRNYLGGIKNWDEHLFHRLSPPSILNYKYWPKNPPQYREVTEEYTKEMQRLTDKFLGFLSEGLGLPRLTLTERIGGDTAEYVLRINFYPPTSHTDLTIGAAPHTDMGVITFLIPNEVPGLQAFKDEQWLDLNYIDTAVIVIIGDQLQRMTNGRFKNVYHRAKTDQEKLRISWPVFIAPRADMVVGPLPELTGKSNPPKFETLVYNDYIDQKIKGWALEDLPVYGNPNDKKNT
>LA_scaffold1381_7.mRNA1
MEKPKFKTVQEVVAAGKGLPERYLFTPTGHGEGEPLNASVPDIALPVMNHGISEAYINKVYELTKQFFALPTEEKEKYAREIGKIQGYGNDLIKFSIGSTVYFSLLTLKINVKLNYGLKSLSDSFDG
>LA_scaffold1588_27.mRNA1
MEVERVQDIASSSLLNEAIPLEFIRSEKDQPATTTFRGPTPAIPVVDLSDPDKESVKRAVVKASEEWGLFQVVNHGIPTELIRKLQDVGKKFFELPSSEKESVAKPEDSKDIEGYGTKISKDLEGKKAWVDHLFHRIWPSSCVNYSFWPKNPPEYREVNEEYAIHVKKLSETLLEFLSEGLGLKREALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGLTLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGKYKNVLHRTTVDKEKTRMSWPVFLEPYREMIVGPLPELTGDNNPPKFKPLAFKDYSYRKLNKLPLD
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University