Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Leavenworthia alabamica
Species name
Leavenworthia alabamica is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Leavenworthia_alabamica.4CL.fa
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>LA_scaffold498_40.mRNA1
MAAGIYHDLGVQQGDVVSLVLPNSIYFPVIFLSLLSLGAIVTTMNPSSSLGEIKKQVSECNVGLAFTSTENVEKLSSLGIMVIRVPESYDFDLIRIENPKFYSLISGGFRFLPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLYHIYGLSLFVLGLLSLGSTIVVMRRFDASDVINVIERFKITHFPVVPPMLMALTKKAKGVCSEVFKSLKQVSSGAAPLNRKFIEDFLGTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPEATQMTIVEDKWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVMMVNSIPKSPAGKILRNELKRLLTISVSSRL
>LA_scaffold1036_20.mRNA1
MAPQQEVNEKQSINSDVIFRSRLPDIYIPNHLSLHDYVFQNISEFASKPCLINGPTGQVYTYSDVHVISRQIAANFHKLGVNQYDVVMILLPNCPEFVLSFLAASFRGAIATXTKLIVTESRYVDKIKSLQNDDGVIIVCTDESETVSIPEGCLRFSELTQSIESDVIDSVVISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNSDDVILCVLPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPIVPPIVLAIAKSSETEKYDLSSIRVVKSGAAPLGKELEDAVSAKFPQAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNKPGEICIRGHQIMKGYLNNPTATLETIDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLFSHQDITDVAVVAMKEESAGEVPVAFVVKSKDSELSEDEVKQFVSKQVVFYKRIYKVFFVDSIPKAPSGKILRKDLRAKLANGL
>LA_scaffold1147_10.mRNA1
MEKSGYGRDGIYRSLRPKLILPKDPNTSLVSFLFRNSSSYPSKPALIDSESGDSLTFAQLKSAVSRLAHGFQRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEISKQIKNSNPKIIISVTQLFDKIKDFNIPIVLLGSNESVHGSKILSFNNVMELSDPVSDLPVVEIKQSDTATLLYSSGTTGTSKGVELTHGNYIAASLMLQRGNALISMSKFELEMVLKNIEKYKVTHLWVVPPVFLALSKQSIVKNYNLSSLKYIGSGAAPLGKDLMEDCGRNIPNVMLMQGYGMTETCGIVSVEDPRLGKRNTGSAGMLAPGVEAQIVSFETGKFQPPNQLGEIWVRGPNMMRGYYNNTQATKETIDKKGWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDEEAGEVPIAFVVRSSNSSVSEEDIQNFIAKQVAPFKRLRRVSFIDIVPKSAAGKILRRELIQKVKSKL
>LA_scaffold1543_7.mRNA1
MTSFSDQNYLIFTSLTTFQSTITSSKTYLSSLLNLAWSTVPXGLRKLGVNQHDVVMLLLPNSPELLLTFLAVSFIGAITTAANPFFTQTEIYKQAKSSAVKLIVTESQYVNKIKILQNDNVSIICTDDSSAIPEGCIRFSELTHSGEEEQNPQVNLETKISPDDVVTLPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPSFYFHREDVILCVLPMFHIFALNSIALCSIRVGATILIMPKFEIGLFLEQIQRSKVTVLIVVPPIILAIVKSQETEKYDLSSVRIIKSGAAPLGKELEDAISAKFPNAKLGQGCGMTETGPIAMSLVFAKQSLPVKSGGCGTVVRNAEMKIVDPNTGDSLPRNKSGEICIRGDQIMKGYLNDPATTASTIDKDGWLHTGDIGYVDDDDELFIIDRLKELIKYKGFQVAPAELESLLIAHPHVNDVAVVAMKDEDVGEVPVAFVVRSKDSNISEDEIKQFISLQVVFYKRIKTVFFTDSIPKAPSGKILRKDLRARLANGLMN
>LA_scaffold2494_3.mRNA1
MALQEEAVMEKQSYNSDVIFRSKLPDIYIPNHLSLHDYVFENISEFSSKPCLINGPTGQVYTYSDVHVISRRIAANFHKLGINQYDVVMILLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIITESRYVDKIKSLQIDDGVIVVCTDESESNQIPQDCLRFSELTQSIESDIINSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCGLRVGAAILIMPKFEIRLLLELIQRCKVTVALIVPPIVKSPETEKYDLSSIRVVKSGAAPLGKELEDAHDVGEPWEGVPGTPGDARDVGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPVTGVSLSRNKPGEIFIRGQQIMKGYLNNPIATLETIDKDGFQVAPAELEALLITHPRIIDVAVVGMKEEAAGEVPVAFVVKSKDSDLSEDEVKQFISKQVVFYKRINKVFFTDSIPKAPSGKILRKYLREKLLNGL
>LA_scaffold2519_5.mRNA1
MITTALHETQIHKPAVTSPPTVVSADASSPSPTPRIFRSKLPDIYIPNHLSLHTYCFEKISSFSDKPCLIVGSTGKHYTYGETHRICRSVAAGLYKMGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQEIYKQLQSSGAKLIITHSHYVDKLRNLGEDLTVITTDDPTPENCLPFSILTDETNPLEESIDVGGEDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNIYLKSNDVVLCVLPLFHIYSLNSVLLNSIRSGATVLLMHKFEIGALLDLIQRHRVTMAALVPPLVIALAKNPAVNSYDLSSVRLVLSGAAPLGKDLQDSLRRRLPQAVLGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHVETRMSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVPPAELESLLINHNSIADAAVVPQRDEVAGEVPVAFVVRSNGNNITEEEIKEYISKQVVFYKRLHKVFFVTSIPKSPAGKILRKDLKAKLSSM
>LA_scaffold2690_4.mRNA1
MEDQKQNNEHIFRSLYPSVPIPDKLTLPEFVLQGVEEFTEKVAFVEAVTGKAVTYGDVVRDTKRLAKALTSLGLRKGHVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGAKGIITDATNFEKVKTLGLPIILLGEEKIEGAVNWKDLLEAGDKSGHNNKEDNNILQTDLCALPFSSGTTGLQKGVMLTHRNLIANLCSSLFSVRSEMIGQIVTLGLIPFFHIYGIVGICCATMKNKGKVVAMSRYDLRIFLNALISHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLRSVMTAAAPLAPELLTAFEAKFPNAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILPNLEVKFIDPETGRSLPKNTSGELCVRSQCVMQGYFKNKEETEKTIDEEGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQKY
>LA_scaffold2920_8.mRNA1
MALQQQTLFLTDQEPSHDFIFRSKLPDISIPSHLPLIDYVFQNFSGDGDCNSTCLIDGSTGRIFTYADVQADLRCIAAGIHRFGIGHRDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIEKQAKASTAKMIITKRCYVDKLINLKDDGVLIVCVEDSDDVVSVDGCVTFTELFQTYETELTKPEISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGDNPNLNFTANDVILCFLPMFHIYALDALMLSAMRTGASLLIVPRFELNLVMELIQKYKVTVVPVAPPVVLAFVKSPDTERYDLTSVRMMLSGAATLKKELEEAVRLKFPNTIFGQGYGMTESGTVAKSLAFAKNPFKTKSGSCGTVLRNTEMKVVDTITGISLPRNQSGEICVRGHQIMKGYLNDPEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPYIDDAAVVAMKDEVADEVPVAFVVRSDGSQLTEDDVKSYVNKQVVHYKRIKTVFFIEAIPKAVSGKILRKELQAKLETKLL
MAAGIYHDLGVQQGDVVSLVLPNSIYFPVIFLSLLSLGAIVTTMNPSSSLGEIKKQVSECNVGLAFTSTENVEKLSSLGIMVIRVPESYDFDLIRIENPKFYSLISGGFRFLPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLYHIYGLSLFVLGLLSLGSTIVVMRRFDASDVINVIERFKITHFPVVPPMLMALTKKAKGVCSEVFKSLKQVSSGAAPLNRKFIEDFLGTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPEATQMTIVEDKWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVMMVNSIPKSPAGKILRNELKRLLTISVSSRL
>LA_scaffold1036_20.mRNA1
MAPQQEVNEKQSINSDVIFRSRLPDIYIPNHLSLHDYVFQNISEFASKPCLINGPTGQVYTYSDVHVISRQIAANFHKLGVNQYDVVMILLPNCPEFVLSFLAASFRGAIATXTKLIVTESRYVDKIKSLQNDDGVIIVCTDESETVSIPEGCLRFSELTQSIESDVIDSVVISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNSDDVILCVLPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPIVPPIVLAIAKSSETEKYDLSSIRVVKSGAAPLGKELEDAVSAKFPQAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNKPGEICIRGHQIMKGYLNNPTATLETIDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLFSHQDITDVAVVAMKEESAGEVPVAFVVKSKDSELSEDEVKQFVSKQVVFYKRIYKVFFVDSIPKAPSGKILRKDLRAKLANGL
>LA_scaffold1147_10.mRNA1
MEKSGYGRDGIYRSLRPKLILPKDPNTSLVSFLFRNSSSYPSKPALIDSESGDSLTFAQLKSAVSRLAHGFQRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEISKQIKNSNPKIIISVTQLFDKIKDFNIPIVLLGSNESVHGSKILSFNNVMELSDPVSDLPVVEIKQSDTATLLYSSGTTGTSKGVELTHGNYIAASLMLQRGNALISMSKFELEMVLKNIEKYKVTHLWVVPPVFLALSKQSIVKNYNLSSLKYIGSGAAPLGKDLMEDCGRNIPNVMLMQGYGMTETCGIVSVEDPRLGKRNTGSAGMLAPGVEAQIVSFETGKFQPPNQLGEIWVRGPNMMRGYYNNTQATKETIDKKGWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDEEAGEVPIAFVVRSSNSSVSEEDIQNFIAKQVAPFKRLRRVSFIDIVPKSAAGKILRRELIQKVKSKL
>LA_scaffold1543_7.mRNA1
MTSFSDQNYLIFTSLTTFQSTITSSKTYLSSLLNLAWSTVPXGLRKLGVNQHDVVMLLLPNSPELLLTFLAVSFIGAITTAANPFFTQTEIYKQAKSSAVKLIVTESQYVNKIKILQNDNVSIICTDDSSAIPEGCIRFSELTHSGEEEQNPQVNLETKISPDDVVTLPYSSGTTGLPKGVMLTHKALVTSVAQQVDGENPSFYFHREDVILCVLPMFHIFALNSIALCSIRVGATILIMPKFEIGLFLEQIQRSKVTVLIVVPPIILAIVKSQETEKYDLSSVRIIKSGAAPLGKELEDAISAKFPNAKLGQGCGMTETGPIAMSLVFAKQSLPVKSGGCGTVVRNAEMKIVDPNTGDSLPRNKSGEICIRGDQIMKGYLNDPATTASTIDKDGWLHTGDIGYVDDDDELFIIDRLKELIKYKGFQVAPAELESLLIAHPHVNDVAVVAMKDEDVGEVPVAFVVRSKDSNISEDEIKQFISLQVVFYKRIKTVFFTDSIPKAPSGKILRKDLRARLANGLMN
>LA_scaffold2494_3.mRNA1
MALQEEAVMEKQSYNSDVIFRSKLPDIYIPNHLSLHDYVFENISEFSSKPCLINGPTGQVYTYSDVHVISRRIAANFHKLGINQYDVVMILLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIITESRYVDKIKSLQIDDGVIVVCTDESESNQIPQDCLRFSELTQSIESDIINSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCGLRVGAAILIMPKFEIRLLLELIQRCKVTVALIVPPIVKSPETEKYDLSSIRVVKSGAAPLGKELEDAHDVGEPWEGVPGTPGDARDVGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPVTGVSLSRNKPGEIFIRGQQIMKGYLNNPIATLETIDKDGFQVAPAELEALLITHPRIIDVAVVGMKEEAAGEVPVAFVVKSKDSDLSEDEVKQFISKQVVFYKRINKVFFTDSIPKAPSGKILRKYLREKLLNGL
>LA_scaffold2519_5.mRNA1
MITTALHETQIHKPAVTSPPTVVSADASSPSPTPRIFRSKLPDIYIPNHLSLHTYCFEKISSFSDKPCLIVGSTGKHYTYGETHRICRSVAAGLYKMGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQEIYKQLQSSGAKLIITHSHYVDKLRNLGEDLTVITTDDPTPENCLPFSILTDETNPLEESIDVGGEDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNIYLKSNDVVLCVLPLFHIYSLNSVLLNSIRSGATVLLMHKFEIGALLDLIQRHRVTMAALVPPLVIALAKNPAVNSYDLSSVRLVLSGAAPLGKDLQDSLRRRLPQAVLGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHVETRMSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVPPAELESLLINHNSIADAAVVPQRDEVAGEVPVAFVVRSNGNNITEEEIKEYISKQVVFYKRLHKVFFVTSIPKSPAGKILRKDLKAKLSSM
>LA_scaffold2690_4.mRNA1
MEDQKQNNEHIFRSLYPSVPIPDKLTLPEFVLQGVEEFTEKVAFVEAVTGKAVTYGDVVRDTKRLAKALTSLGLRKGHVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGAKGIITDATNFEKVKTLGLPIILLGEEKIEGAVNWKDLLEAGDKSGHNNKEDNNILQTDLCALPFSSGTTGLQKGVMLTHRNLIANLCSSLFSVRSEMIGQIVTLGLIPFFHIYGIVGICCATMKNKGKVVAMSRYDLRIFLNALISHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLRSVMTAAAPLAPELLTAFEAKFPNAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILPNLEVKFIDPETGRSLPKNTSGELCVRSQCVMQGYFKNKEETEKTIDEEGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQKY
>LA_scaffold2920_8.mRNA1
MALQQQTLFLTDQEPSHDFIFRSKLPDISIPSHLPLIDYVFQNFSGDGDCNSTCLIDGSTGRIFTYADVQADLRCIAAGIHRFGIGHRDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIEKQAKASTAKMIITKRCYVDKLINLKDDGVLIVCVEDSDDVVSVDGCVTFTELFQTYETELTKPEISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGDNPNLNFTANDVILCFLPMFHIYALDALMLSAMRTGASLLIVPRFELNLVMELIQKYKVTVVPVAPPVVLAFVKSPDTERYDLTSVRMMLSGAATLKKELEEAVRLKFPNTIFGQGYGMTESGTVAKSLAFAKNPFKTKSGSCGTVLRNTEMKVVDTITGISLPRNQSGEICVRGHQIMKGYLNDPEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPYIDDAAVVAMKDEVADEVPVAFVVRSDGSQLTEDDVKSYVNKQVVHYKRIKTVFFIEAIPKAVSGKILRKELQAKLETKLL
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University