Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Laqenaria siceraria
Species name
White-flowered gourd (Lagenaria siceraria) is a species of eudicot in the family Cucurbitaceae (cucumber family).
Species info
Gene sequence info
Laqenaria_siceraria.COMT.fa
Download
>Lsi02G018830.1
MGKSGCFLLILLLGLMLLEKKIVEGNKVNVVPSIIVFGDSSVDSGNNNHISTVLRSDFAPYGRDFEGGKATGRFCNGKIVTDFISEAFGIKPTIPAYLDPSYNITHFASGVCFASAGTGYDNATSGIFSVIPLWKELQYYREYQKKLRDYLGPSKANTTISQSLYLISLGTNDFLENYFLLPPRSSEFSLQDYQNFLARAAEGFVRELYALGARKMSIGGLPPMGCLPLERSSTIIFGGGGECVEKYNKVARDFNAKLMGLVEMMKEELKGIKIVFSNPFDVLYDMIQHPSYFVLYLPLRRWILHDWSDDHCVKLLKNCYIAIPNDGKVIIVDSVVPTVPETTNAIKSIAQCDVLMMTQNPGGKERTQDEFKALATKAGFKHVIFQCFLSDIWVIEFLKN
>Lsi03G019670.1
MEQDNAAATTAESESQNKSRLAILELANMISVPMSLNAIVRLNVADAIWQEGSNSPLSASEILARGVPSGGDADNLERILRMLTSYGVFEEHLSPNSSNRRYSLTDVGKTLVKDSDGLSYAPYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEPAYSYYGKKAEMNELMQRAMAGVSVPFMKAVLDGYDGFKGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGVSHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKIILENCCKSLPAGGKLIACEPTLPEKTDDSHRTRALLASDIFIMTIYKAKSKQRTEEQFRQLGLSAGFSALRPFHIDYFYCLLEFQK
>Lsi07G003220.1
MGSLVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGRGGEGAVLSPSQIASQLSGAKNPEAHVMLDRMLRLLASYSILTYSLNPLPDGSVERLYGLAPVSKFLIKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMSAFEYHGTDPRFNKVFNRGMSEHSTITMKKILETYKGFGGLNSVVDVGGGTGAVLNMIVSKYPSIKGINFDLPHVIQDAPAYPGVDHVGGDMFDSVPKGDAIFMKWICHDWSDQHCLKFLKNCYDALPEHGKVIVAECILPVAPDSSLATKGVIHIDLIMLAHNPGGKERTEKEFEALSKAAGFHGFKVHCCAFNTYVMEFLKTP
>Lsi07G014530.1
MASTAEETTITFSSSDATHEQDYAYAMQLATSSVVPMTLQAAFELGVFEILAKAGNGAELSSAEIAAEITTTNPEAATMIDRMLRLLASHSVVGCSLAFDEDGNAQRLYSLAPVSNYYVRNEDGVSLGPLLSLLQDEVLLHSWTALKNAVIEGGGIFNRAHGGIHAFEYLSLDPRFNQVFNTAMINHTTMPIKQIVKSYKGFANIKQLVDVGGGLGVTLQVITSTYPSIKGINFDLPHVLRDAPSYPGVEHVGGDMFEKLPKGDAVFMKWILHDWSDNHCVKLLKNCYTAIPNDGKVIIVDSVLPTIPDSTNATKATAQCDMIVMTLYQGGKERTQDEFKALATQAGFKHVIFQCLVSNLWVIEFFKDFIPHQD
>Lsi07G014540.1
MASTAEETATTFSSNAAAQEQQHYAYAMQLVTLSVLPMTLQAAFELGVFEILAKAGDGAKLSSVEIATKITTTNPDAASMIDRMLRLLASHSVVGCSMGFDEDEKMRRVYSLTPVSKCYVCNEDGVSLGPILSLIQDQVFLCSWSELKNAVIEGGVPFNRAHEGVHAFDYPGLDPRFNQVFNTAMINHTTMVIKKIVNSYKGFANIKQLVDVGGGLGVTLQIITSKYPSIKGINFDLPHVIHNAPSYPGVEHVGGDMFKKVPNGNAVFMKWILHDWSDDHCVKLLKNCYTAIPNDGKEIIVDSVVPTVPETTNVTKSIAQFDVLMMTQNPGGKERTQDEFKALATKAKFKRVIFQCFVSNLWVIEFLKN
>Lsi07G014550.1
MASMAEKTTIVTSSSDDAVQEQQHHAYAMQLVTSSVLPMTLQAAFELSVFEILAKAGDGAELSPAEIAAEITMTNPDAASMIDRMLWLLASHSVVGCTVGFDENGKMQRLYSLTPVSKYFVRNEDGVSLGPTLLLIQDKVFFHSWSELKNAVIEGGVPFNRAHGGVHAFEYPSLDPRFNQVFNTAMINHTTTCIKKIVKFYKGFTNIKQLVDVGGGLGVTLQIITSAYPSIKGINFDLPHVICNAPSHPGNNFDYDLTDVEHVGGDMFEKVPHGDAVFMKWILHDWSDDHCVKLLKNCYIAIPNDGKVIIVDSVVPTVPETTNAIKSIAQCDVLMMTQNPGGKERTQDEFKALATKAGFKHVIFQCFLSDIWVIEFLKN
MGKSGCFLLILLLGLMLLEKKIVEGNKVNVVPSIIVFGDSSVDSGNNNHISTVLRSDFAPYGRDFEGGKATGRFCNGKIVTDFISEAFGIKPTIPAYLDPSYNITHFASGVCFASAGTGYDNATSGIFSVIPLWKELQYYREYQKKLRDYLGPSKANTTISQSLYLISLGTNDFLENYFLLPPRSSEFSLQDYQNFLARAAEGFVRELYALGARKMSIGGLPPMGCLPLERSSTIIFGGGGECVEKYNKVARDFNAKLMGLVEMMKEELKGIKIVFSNPFDVLYDMIQHPSYFVLYLPLRRWILHDWSDDHCVKLLKNCYIAIPNDGKVIIVDSVVPTVPETTNAIKSIAQCDVLMMTQNPGGKERTQDEFKALATKAGFKHVIFQCFLSDIWVIEFLKN
>Lsi03G019670.1
MEQDNAAATTAESESQNKSRLAILELANMISVPMSLNAIVRLNVADAIWQEGSNSPLSASEILARGVPSGGDADNLERILRMLTSYGVFEEHLSPNSSNRRYSLTDVGKTLVKDSDGLSYAPYVLQHHQDALMRAWPRVHEAAIDATTEPFVRANGEPAYSYYGKKAEMNELMQRAMAGVSVPFMKAVLDGYDGFKGVEKLVDVGGSAGDCLRMILQKHPHIKEGINFDLPEVVARAPSIPGVSHVGGDMFKSVPTGDAIFMKWVLSTWTDDECKIILENCCKSLPAGGKLIACEPTLPEKTDDSHRTRALLASDIFIMTIYKAKSKQRTEEQFRQLGLSAGFSALRPFHIDYFYCLLEFQK
>Lsi07G003220.1
MGSLVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGRGGEGAVLSPSQIASQLSGAKNPEAHVMLDRMLRLLASYSILTYSLNPLPDGSVERLYGLAPVSKFLIKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMSAFEYHGTDPRFNKVFNRGMSEHSTITMKKILETYKGFGGLNSVVDVGGGTGAVLNMIVSKYPSIKGINFDLPHVIQDAPAYPGVDHVGGDMFDSVPKGDAIFMKWICHDWSDQHCLKFLKNCYDALPEHGKVIVAECILPVAPDSSLATKGVIHIDLIMLAHNPGGKERTEKEFEALSKAAGFHGFKVHCCAFNTYVMEFLKTP
>Lsi07G014530.1
MASTAEETTITFSSSDATHEQDYAYAMQLATSSVVPMTLQAAFELGVFEILAKAGNGAELSSAEIAAEITTTNPEAATMIDRMLRLLASHSVVGCSLAFDEDGNAQRLYSLAPVSNYYVRNEDGVSLGPLLSLLQDEVLLHSWTALKNAVIEGGGIFNRAHGGIHAFEYLSLDPRFNQVFNTAMINHTTMPIKQIVKSYKGFANIKQLVDVGGGLGVTLQVITSTYPSIKGINFDLPHVLRDAPSYPGVEHVGGDMFEKLPKGDAVFMKWILHDWSDNHCVKLLKNCYTAIPNDGKVIIVDSVLPTIPDSTNATKATAQCDMIVMTLYQGGKERTQDEFKALATQAGFKHVIFQCLVSNLWVIEFFKDFIPHQD
>Lsi07G014540.1
MASTAEETATTFSSNAAAQEQQHYAYAMQLVTLSVLPMTLQAAFELGVFEILAKAGDGAKLSSVEIATKITTTNPDAASMIDRMLRLLASHSVVGCSMGFDEDEKMRRVYSLTPVSKCYVCNEDGVSLGPILSLIQDQVFLCSWSELKNAVIEGGVPFNRAHEGVHAFDYPGLDPRFNQVFNTAMINHTTMVIKKIVNSYKGFANIKQLVDVGGGLGVTLQIITSKYPSIKGINFDLPHVIHNAPSYPGVEHVGGDMFKKVPNGNAVFMKWILHDWSDDHCVKLLKNCYTAIPNDGKEIIVDSVVPTVPETTNVTKSIAQFDVLMMTQNPGGKERTQDEFKALATKAKFKRVIFQCFVSNLWVIEFLKN
>Lsi07G014550.1
MASMAEKTTIVTSSSDDAVQEQQHHAYAMQLVTSSVLPMTLQAAFELSVFEILAKAGDGAELSPAEIAAEITMTNPDAASMIDRMLWLLASHSVVGCTVGFDENGKMQRLYSLTPVSKYFVRNEDGVSLGPTLLLIQDKVFFHSWSELKNAVIEGGVPFNRAHGGVHAFEYPSLDPRFNQVFNTAMINHTTTCIKKIVKFYKGFTNIKQLVDVGGGLGVTLQIITSAYPSIKGINFDLPHVICNAPSHPGNNFDYDLTDVEHVGGDMFEKVPHGDAVFMKWILHDWSDDHCVKLLKNCYIAIPNDGKVIIVDSVVPTVPETTNAIKSIAQCDVLMMTQNPGGKERTQDEFKALATKAGFKHVIFQCFLSDIWVIEFLKN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University