Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Kobresia littledalei
Species name
Carex littledalei is a species of monocot in the family Cyperaceae (sedge family).
Species info
Gene sequence info
Kobresia_littledalei.ANR.fa
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>rna-gnl|WGS:SWLB|mrna.FCM35_KLT00115
METKARVCVTGASGFIGSWLVKKLLEKGYVVHATVRNLGDEDKVGLLKSLPEDHFHQYKDTTEAALGGISTILRLCEQSGTVRRVVYTGSITAASPLKEDSAGYNDCFDESCWTSLGVSLPCFSDNFKAYIRSKTLSEKEILMYNSKEGNRKFEVVSLTCGLVGGDTILPYASESMHMIISPLSGIEVFHNGLKALHALFASIPLVHVEDVCEAHVFCIEQPVMAGRFFCATDYPTMKHFVDYFAKEFPELKLIKEVEGKGKSLKASSNKLVDSGFKYKYSVDEVLSETVKCCKRLRTL
>rna-gnl|WGS:SWLB|mrna.FCM35_KLT00114
MEKKSRVCVTGASGFIGSSLVKKLLEKGYVVHATLRNLGDESNYKVGLLKGLPGADTRLFLFEADINDPDTFGPAIEGCEFVFLVATPLVHEPHKTKCKDTTEASVQGIHAILKFCEKSGTVRRVVYTGSVVATSPWKEDFTGYKDFIDESNWTIIDLPYPHYNDHLKGYTSSKTLSEKEILKYNKEGEGGLEVVSLDCGLVGGDTILPYTPDSVLVIVSPLTGIELYHTSLKCLQGLLCSVPLVHIDDVCDAHIFCIERPVMAGRFLCAVDCPTMKDFVDYFAEKFPDLKLIKEVEGEGNRVKGCSQKLVDLGFKYNFGVEEILSTSVECAKRLGGL
>rna-gnl|WGS:SWLB|mrna.FCM35_KLT17648
MKRVCVTGTTGYIGSWLVRSLLQQGYHVHATARDPEKAQRMLSTIEGTDRLQIYRADLNEDRSFDEVVKGCVGLFHVAALMEFGTSVQENVEDHVRRNILEPAVLGTRNLLQSCMRSGTIKRVVFTSSISTITAKDEKGDWTPVVDESSVTPTDLVWKTKNSGWVYVLLKLATEEEAFKFAKENGIDLVSIIPPTVAGSFLTPTVPSSLQVLLSPITGDPNFYPVLDSVHSRLGSIALVHIEDICRAHIFLFETSGASGRYLFKEEFNRSAPSIISSKRLLDLGYRFEYDAKDVIKDSIQSSIGNGFLNSQVEFKV
>rna-gnl|WGS:SWLB|mrna.FCM35_KLT04102
MSEKVVCVTGGSGYIASWLVKLLLQRGYTVRASVRDPSDPKKTEHLRALDGASERLHLFKADLLEEGSFDAAIAGCVGVFHTASPCFFNATDFQAELVDPAVKGTLNVLRSCAKASSVKRVVMTSSMAAVNFNERALSPDVVVDETWFSDPDFCLRNKLWYHLSKTLAEEAAWKFSKENGVELVTINPGMVIGPLLQPTLNLSSSYIYNKINGSTTFPNSSFRWIHVKDVAMAHILAYEIHSANGRYCLVERVAHHSEIVEIIRKLYPSIPVPEKCADDKPFETVYQVSKEKARSLGLNFTPLEQSVKETIESLKEKADPKKTAHLRALGGANERLHLFKANLLEEGSFDSGIEGCVGVFHTASPFYHNVTDPQAELIDPAVKGTLNVLRSCVKYPSIKRVVVTSSMAAVAYNGKPRTPDVVVDETWFSSPKMCEQSKLWYVLSKTLAEEAAWKFAKENGIDIVTINPAMVIGPLLQSTLNTSAAAILNLINGASTYPNTAFGWVNVKDVAMTHILAYEVPSASGRYCLVDKVAHYSEIVEMIRELYPSITLPEKCADDKPFVSIYQVSKEKTKSLGIDYVPTKIGVKETIDSLKEKGFVNF
>rna-gnl|WGS:SWLB|mrna.FCM35_KLT04103
MMSGKEVVCVTGASGYIASWLVKLLLHRGYTVRATVRDPSDPKKTEHLRTLDGAGERLHLFKADLLEEGSFDDVIDGCAGVFHTASPFYLSVTDPQAELIDPAVKGTLNVLKSCAKASSVKRVIVTSSMVAVEQNGRPKNPDVVVDETWFSIPEFCEKSKLWYPLSKTLAEEAAWKFSKENGIDIVTINPGMVIGPLLQPILNTSSATILNLINGSKTFPNASVRWVNVKDVALAHILAYEVPSASGRYCLVERVAHYSEVVEIIRDLYPSIELPEKCADDKPYAPIYQVSKERAKSLGVNFSPLETSIKETIESLRDKADPKRTKHLRELDGASERLHLFKADLMDEGSFDAAIDGCEGVFHAASPVLFTVTYPKAELIDPAVKGTLNVLRSCTKAATVKRVVMTSSMAAVSNNGRPRTPEAVIDETWFSIPEICENLKLWYALSKTLAEEAAWKFSKDNGIDMVTLNPAMVIGPLLQPTLNATCVDIFNLINGSPTYPNLNFGWIHVKDVATAHILAFEVPSARGRYCLVERVLHFSEIVAIIHELYPSLQLPNKCADDKPFVPPYYFSNEKVKELGLGNLIPFKSSLKDTIESLREKGFISF
>rna-gnl|WGS:SWLB|mrna.FCM35_KLT08718
MTMDKKLVCVTGGNGYIASMLIKQLLQKGYAVNTTVRNPDDLERTSHLKEMQSIGPLKIIRGDLDEEGSFDEAVAGCQFVFLVAAPVNLMSQNPEKELIEPAIQGTLNVLKSCAKAKSVKRVVLTSSAAAVSMKSLEGNGHVLDEEAWSDVEFLRTTKPPTWGYGVAKVLAEEAAFKFGEENNLSVVTMLPTLTIGPFLMPTVNTSIALSLSLLTGQEELINGLKGMQMISGSISIVHVEDICRAHIFVAENPAASGRYICCAVNTNLVELAQFLSKRYPQYNVPTDFGDFPEKAKLILSSEKLLKAGFEFNYKHIEEIYDDVVEYAKSKVILK
METKARVCVTGASGFIGSWLVKKLLEKGYVVHATVRNLGDEDKVGLLKSLPEDHFHQYKDTTEAALGGISTILRLCEQSGTVRRVVYTGSITAASPLKEDSAGYNDCFDESCWTSLGVSLPCFSDNFKAYIRSKTLSEKEILMYNSKEGNRKFEVVSLTCGLVGGDTILPYASESMHMIISPLSGIEVFHNGLKALHALFASIPLVHVEDVCEAHVFCIEQPVMAGRFFCATDYPTMKHFVDYFAKEFPELKLIKEVEGKGKSLKASSNKLVDSGFKYKYSVDEVLSETVKCCKRLRTL
>rna-gnl|WGS:SWLB|mrna.FCM35_KLT00114
MEKKSRVCVTGASGFIGSSLVKKLLEKGYVVHATLRNLGDESNYKVGLLKGLPGADTRLFLFEADINDPDTFGPAIEGCEFVFLVATPLVHEPHKTKCKDTTEASVQGIHAILKFCEKSGTVRRVVYTGSVVATSPWKEDFTGYKDFIDESNWTIIDLPYPHYNDHLKGYTSSKTLSEKEILKYNKEGEGGLEVVSLDCGLVGGDTILPYTPDSVLVIVSPLTGIELYHTSLKCLQGLLCSVPLVHIDDVCDAHIFCIERPVMAGRFLCAVDCPTMKDFVDYFAEKFPDLKLIKEVEGEGNRVKGCSQKLVDLGFKYNFGVEEILSTSVECAKRLGGL
>rna-gnl|WGS:SWLB|mrna.FCM35_KLT17648
MKRVCVTGTTGYIGSWLVRSLLQQGYHVHATARDPEKAQRMLSTIEGTDRLQIYRADLNEDRSFDEVVKGCVGLFHVAALMEFGTSVQENVEDHVRRNILEPAVLGTRNLLQSCMRSGTIKRVVFTSSISTITAKDEKGDWTPVVDESSVTPTDLVWKTKNSGWVYVLLKLATEEEAFKFAKENGIDLVSIIPPTVAGSFLTPTVPSSLQVLLSPITGDPNFYPVLDSVHSRLGSIALVHIEDICRAHIFLFETSGASGRYLFKEEFNRSAPSIISSKRLLDLGYRFEYDAKDVIKDSIQSSIGNGFLNSQVEFKV
>rna-gnl|WGS:SWLB|mrna.FCM35_KLT04102
MSEKVVCVTGGSGYIASWLVKLLLQRGYTVRASVRDPSDPKKTEHLRALDGASERLHLFKADLLEEGSFDAAIAGCVGVFHTASPCFFNATDFQAELVDPAVKGTLNVLRSCAKASSVKRVVMTSSMAAVNFNERALSPDVVVDETWFSDPDFCLRNKLWYHLSKTLAEEAAWKFSKENGVELVTINPGMVIGPLLQPTLNLSSSYIYNKINGSTTFPNSSFRWIHVKDVAMAHILAYEIHSANGRYCLVERVAHHSEIVEIIRKLYPSIPVPEKCADDKPFETVYQVSKEKARSLGLNFTPLEQSVKETIESLKEKADPKKTAHLRALGGANERLHLFKANLLEEGSFDSGIEGCVGVFHTASPFYHNVTDPQAELIDPAVKGTLNVLRSCVKYPSIKRVVVTSSMAAVAYNGKPRTPDVVVDETWFSSPKMCEQSKLWYVLSKTLAEEAAWKFAKENGIDIVTINPAMVIGPLLQSTLNTSAAAILNLINGASTYPNTAFGWVNVKDVAMTHILAYEVPSASGRYCLVDKVAHYSEIVEMIRELYPSITLPEKCADDKPFVSIYQVSKEKTKSLGIDYVPTKIGVKETIDSLKEKGFVNF
>rna-gnl|WGS:SWLB|mrna.FCM35_KLT04103
MMSGKEVVCVTGASGYIASWLVKLLLHRGYTVRATVRDPSDPKKTEHLRTLDGAGERLHLFKADLLEEGSFDDVIDGCAGVFHTASPFYLSVTDPQAELIDPAVKGTLNVLKSCAKASSVKRVIVTSSMVAVEQNGRPKNPDVVVDETWFSIPEFCEKSKLWYPLSKTLAEEAAWKFSKENGIDIVTINPGMVIGPLLQPILNTSSATILNLINGSKTFPNASVRWVNVKDVALAHILAYEVPSASGRYCLVERVAHYSEVVEIIRDLYPSIELPEKCADDKPYAPIYQVSKERAKSLGVNFSPLETSIKETIESLRDKADPKRTKHLRELDGASERLHLFKADLMDEGSFDAAIDGCEGVFHAASPVLFTVTYPKAELIDPAVKGTLNVLRSCTKAATVKRVVMTSSMAAVSNNGRPRTPEAVIDETWFSIPEICENLKLWYALSKTLAEEAAWKFSKDNGIDMVTLNPAMVIGPLLQPTLNATCVDIFNLINGSPTYPNLNFGWIHVKDVATAHILAFEVPSARGRYCLVERVLHFSEIVAIIHELYPSLQLPNKCADDKPFVPPYYFSNEKVKELGLGNLIPFKSSLKDTIESLREKGFISF
>rna-gnl|WGS:SWLB|mrna.FCM35_KLT08718
MTMDKKLVCVTGGNGYIASMLIKQLLQKGYAVNTTVRNPDDLERTSHLKEMQSIGPLKIIRGDLDEEGSFDEAVAGCQFVFLVAAPVNLMSQNPEKELIEPAIQGTLNVLKSCAKAKSVKRVVLTSSAAAVSMKSLEGNGHVLDEEAWSDVEFLRTTKPPTWGYGVAKVLAEEAAFKFGEENNLSVVTMLPTLTIGPFLMPTVNTSIALSLSLLTGQEELINGLKGMQMISGSISIVHVEDICRAHIFVAENPAASGRYICCAVNTNLVELAQFLSKRYPQYNVPTDFGDFPEKAKLILSSEKLLKAGFEFNYKHIEEIYDDVVEYAKSKVILK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University