Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Kalanchoe fedtschenkoi
Species name
South american air plant (Kalanchoe fedtschenkoi) is a species of eudicot in the family Crassulaceae (stonecrop family).
Species info
Gene sequence info
Kalanchoe_fedtschenkoi.FLS.fa
Download
>Kaladp0011s1169.1.v1.1
MEVVRVQAIASSAMLKDTIPSEFIRCESEQPAITTVQGASLEVPVISFNEADEEKLVKQVAEASREWGLFQIVNHEIPSEAIKKLQDVGKGFFELPLEEKE
>Kaladp0020s0030.1.v1.1
MELPVIDLSPILAVGPTDPQSEKLCEVVSRTLRETGALLVKDPRCSAEDNDRFIDMMERYFQQPPDFKRLQERPYLHYQVGVTPEGVEVPRSFVDPQMQKKLKAMPEEFQPATPRGPDPKWRYMWRVGPRPSDTRFQELNSEPVIPEGFPEWKETMDSWGYKMISAIEVVAEMAAIGFGLPRDAFTSLMKQGPHLLAPTGSDLSCYGQEGFVFAGYHYDLNFLTIHGKSRFPGLNIWLRRGQKVEVKVPVGCLLIQAGKQIEWLTGGECIAGMHEVVVTKRTVDAIKAAQDQNRSLWRVSSTLFAHIASDNVLKPLGHYVKSEYASQYPPIVAGEYVEQELSIINLRGKAGDGDNLEN
>Kaladp0024s0402.1.v1.1
MSTKTLQTWPEPVISVQTLSESGVNTIPNRYIKPMHDRPDALADDTLTAIPVVNLSHIFSDDSSVRGDTMRMIDKACREWGFFQVVDHGVSHELMGRMLGSWREFFKLPVEVKQVHANSPATYEGYGSRVGVEKEAVLDWCDYFYLHFYPRSVRDPSKWPSLPASCRWVIKSAVSIISIQPLELAEKLIDALDREVLAEYGEALADLSGRLMTIFSSNLGLTQGYLLEAFGERSACLRANFYPKCPQPNLTLGLSPHSDPGGLTLLLADPTVAGLQIRKGNNWVTVRPLPNAFIVNLGDQIQLLSNGIYRSVEHRTTVHSEHERISIAFFYNPEGDALVQPANELVTDQRPARYPAMTFNEYRSFIRCKGLRGKSQVESLKLPNQLRSTSLT
>Kaladp0040s0073.1.v1.1
MAPTMNDVQRVQALAEGKIQELPPQFIRPAAERPMNTRALEGVKVPIVSLLSQPPEELVRQVHEACCEWGFFLLVDHGIPSDLLMSLQQVGKDFFGLPMQVKESYANDASKGQYEGYGTKMTKILDEKIEWIDYFFHVLAPAQKVNYDFWPKKPESYRKVNEEYTKALKPVMDKIFELLSQGLGLEAEALKRSCGGENIELEMKINMYPPCPQPELALGVQAHTDMSALTLLISNDVPGLQVWKDNNWVAAGYTPDALFVHIGDQVEVLSNGKYKSVLHRSLVSKDQVRMSWAVFCGPPHETVIGPLPQLLDERNPPKYSTKTYAEYRHRKFNRLPQ
>Kaladp0043s0026.1.v1.1
MEVHVQEIASKSLSEGTIPSEFVRTENEQPGITTFHGPITSPPIIDLSEKDEKRVGEKMAKAASEWGLFQIVRHDIPDIIIHKLQQVGREFFELSQEEKEKYAKPPEAKSIEGYGTKLQKEVDGKKGWVDHLFHKIWPPNAINHQFWPQHSPQYREANEKYAECLHGVVNKLFRLLGEGLGMEGRELQEAVGGENLVYLLKINYYPPCPRPDVALGVPPHTDMSSLTILVPNDVKGLQVFRDDHWFDVQYIPNALIIHIGDQLEIVSNGKYKAVFHRTTVDKDKTRMSWPVFLEPPPEMEVGPHPKAMDDNKPAKYKKKKYADYCYCKLNKIPQ
>Kaladp0046s0298.1.v1.1
MHLHGVVNKLLRALSLGLGLEENEVREALGGEELIYLLKINYYPPCPRPDLALGVVAHTDMSGITILVPNDVQGLQVFRDGHWYDVEYIPNALIIHIGDQLEVVSNGKYKAVLHRSTVNKEKTRISWPVFVEPPEDKEVGPHPKLVTNENPAKYNTKTYQEYKHRKLNKLLNNLKLPYISKTINNL
>Kaladp0099s0109.1.v1.1
MELSVIDLAPYLSANSAEAESAAAKLCSQVSGALKATGALLVRDPRCAAELNDRFIDLMERYFDMPPEFKRLQERPELRYQAGVLPEGVEVPRVLVDRAMQEELERVSDDAKPIIPKGPDNKCRYMWRIGARPLVTRFKELNSDPVIPEGFDEWKETMNSWGYKIISTVEVVAEMAAIGFGLPKDAFTSLMKQGPHKLAPTGSNLQVYGQEGTVLAGYHYDLNFLTIHGRSRFPGLNIWYRNGQKVEVKVPEGCLLIQAGKQIEYLTAGECSAGMHEVVVTRKTVEAIKLAVEQNRSLWRVSSTLFAHIASDALLKPLGHFAELPSADAYPPIHTGDFVSREIAVIYSKPSK
>Kaladp0515s0029.1.v1.1
MEVVRVQAIASSAMPKDTIPSEFIRCESEQPAITTVQGASLEVPVISFDEADEEKLVKQVAEASREWGLFQIVSHEIPSEAIQKLQDVGKGFFELPLEEKEKYAKVADSTSSEEGYGTKLQKEVEGKKGWVDHLFHKIWPPAARNYRFWPLNPPTYREANEEYTMHLHGVVNKLLRALSLGLGLEENEVKEALGGEELIYLLKINYYPPCPRPDLALGVVAHTDMSGITILVPNDVQGLQVFRDGHWYDVEYIPNALIIHIGDQLEVVSNGKYKAVLHRSTVNKEKTRISWPVFVEPPEDKEVGPHPKLVTDENPAKYNTKTYQEYKHRKLNKLPQ
>Kaladp0515s0028.1.v1.1
MEVVRVQAIASSAMPKDTIPSEFIRCESEQPAITTVQGASLEVPVISFNEADEEKLVKQVAEASREWGLFQIVNHEIPSEAIKKLQDVGKGFFELPLEEKEKYAKAADSISAEEGYGTKLQKEVEGKKGWVDHLFHKIWPPAARNYRFWPLNPPTYRKANEEYTMHLHGVVNKLLRALSLGLGLEENEVKEALGGEELIYLLKINYYPPCPRPDLALGVVAHTDMSGITILVPNDVQGLQVFRDGHWYDVEYIPNALIIHIGDQLEVVSNGKYKAVLHRSTVNKEKTRISWPVFVEPPEDKEVGPHPKLVTNENPAKYNTKTYREYKHRKLNKLPQ
MEVVRVQAIASSAMLKDTIPSEFIRCESEQPAITTVQGASLEVPVISFNEADEEKLVKQVAEASREWGLFQIVNHEIPSEAIKKLQDVGKGFFELPLEEKE
>Kaladp0020s0030.1.v1.1
MELPVIDLSPILAVGPTDPQSEKLCEVVSRTLRETGALLVKDPRCSAEDNDRFIDMMERYFQQPPDFKRLQERPYLHYQVGVTPEGVEVPRSFVDPQMQKKLKAMPEEFQPATPRGPDPKWRYMWRVGPRPSDTRFQELNSEPVIPEGFPEWKETMDSWGYKMISAIEVVAEMAAIGFGLPRDAFTSLMKQGPHLLAPTGSDLSCYGQEGFVFAGYHYDLNFLTIHGKSRFPGLNIWLRRGQKVEVKVPVGCLLIQAGKQIEWLTGGECIAGMHEVVVTKRTVDAIKAAQDQNRSLWRVSSTLFAHIASDNVLKPLGHYVKSEYASQYPPIVAGEYVEQELSIINLRGKAGDGDNLEN
>Kaladp0024s0402.1.v1.1
MSTKTLQTWPEPVISVQTLSESGVNTIPNRYIKPMHDRPDALADDTLTAIPVVNLSHIFSDDSSVRGDTMRMIDKACREWGFFQVVDHGVSHELMGRMLGSWREFFKLPVEVKQVHANSPATYEGYGSRVGVEKEAVLDWCDYFYLHFYPRSVRDPSKWPSLPASCRWVIKSAVSIISIQPLELAEKLIDALDREVLAEYGEALADLSGRLMTIFSSNLGLTQGYLLEAFGERSACLRANFYPKCPQPNLTLGLSPHSDPGGLTLLLADPTVAGLQIRKGNNWVTVRPLPNAFIVNLGDQIQLLSNGIYRSVEHRTTVHSEHERISIAFFYNPEGDALVQPANELVTDQRPARYPAMTFNEYRSFIRCKGLRGKSQVESLKLPNQLRSTSLT
>Kaladp0040s0073.1.v1.1
MAPTMNDVQRVQALAEGKIQELPPQFIRPAAERPMNTRALEGVKVPIVSLLSQPPEELVRQVHEACCEWGFFLLVDHGIPSDLLMSLQQVGKDFFGLPMQVKESYANDASKGQYEGYGTKMTKILDEKIEWIDYFFHVLAPAQKVNYDFWPKKPESYRKVNEEYTKALKPVMDKIFELLSQGLGLEAEALKRSCGGENIELEMKINMYPPCPQPELALGVQAHTDMSALTLLISNDVPGLQVWKDNNWVAAGYTPDALFVHIGDQVEVLSNGKYKSVLHRSLVSKDQVRMSWAVFCGPPHETVIGPLPQLLDERNPPKYSTKTYAEYRHRKFNRLPQ
>Kaladp0043s0026.1.v1.1
MEVHVQEIASKSLSEGTIPSEFVRTENEQPGITTFHGPITSPPIIDLSEKDEKRVGEKMAKAASEWGLFQIVRHDIPDIIIHKLQQVGREFFELSQEEKEKYAKPPEAKSIEGYGTKLQKEVDGKKGWVDHLFHKIWPPNAINHQFWPQHSPQYREANEKYAECLHGVVNKLFRLLGEGLGMEGRELQEAVGGENLVYLLKINYYPPCPRPDVALGVPPHTDMSSLTILVPNDVKGLQVFRDDHWFDVQYIPNALIIHIGDQLEIVSNGKYKAVFHRTTVDKDKTRMSWPVFLEPPPEMEVGPHPKAMDDNKPAKYKKKKYADYCYCKLNKIPQ
>Kaladp0046s0298.1.v1.1
MHLHGVVNKLLRALSLGLGLEENEVREALGGEELIYLLKINYYPPCPRPDLALGVVAHTDMSGITILVPNDVQGLQVFRDGHWYDVEYIPNALIIHIGDQLEVVSNGKYKAVLHRSTVNKEKTRISWPVFVEPPEDKEVGPHPKLVTNENPAKYNTKTYQEYKHRKLNKLLNNLKLPYISKTINNL
>Kaladp0099s0109.1.v1.1
MELSVIDLAPYLSANSAEAESAAAKLCSQVSGALKATGALLVRDPRCAAELNDRFIDLMERYFDMPPEFKRLQERPELRYQAGVLPEGVEVPRVLVDRAMQEELERVSDDAKPIIPKGPDNKCRYMWRIGARPLVTRFKELNSDPVIPEGFDEWKETMNSWGYKIISTVEVVAEMAAIGFGLPKDAFTSLMKQGPHKLAPTGSNLQVYGQEGTVLAGYHYDLNFLTIHGRSRFPGLNIWYRNGQKVEVKVPEGCLLIQAGKQIEYLTAGECSAGMHEVVVTRKTVEAIKLAVEQNRSLWRVSSTLFAHIASDALLKPLGHFAELPSADAYPPIHTGDFVSREIAVIYSKPSK
>Kaladp0515s0029.1.v1.1
MEVVRVQAIASSAMPKDTIPSEFIRCESEQPAITTVQGASLEVPVISFDEADEEKLVKQVAEASREWGLFQIVSHEIPSEAIQKLQDVGKGFFELPLEEKEKYAKVADSTSSEEGYGTKLQKEVEGKKGWVDHLFHKIWPPAARNYRFWPLNPPTYREANEEYTMHLHGVVNKLLRALSLGLGLEENEVKEALGGEELIYLLKINYYPPCPRPDLALGVVAHTDMSGITILVPNDVQGLQVFRDGHWYDVEYIPNALIIHIGDQLEVVSNGKYKAVLHRSTVNKEKTRISWPVFVEPPEDKEVGPHPKLVTDENPAKYNTKTYQEYKHRKLNKLPQ
>Kaladp0515s0028.1.v1.1
MEVVRVQAIASSAMPKDTIPSEFIRCESEQPAITTVQGASLEVPVISFNEADEEKLVKQVAEASREWGLFQIVNHEIPSEAIKKLQDVGKGFFELPLEEKEKYAKAADSISAEEGYGTKLQKEVEGKKGWVDHLFHKIWPPAARNYRFWPLNPPTYRKANEEYTMHLHGVVNKLLRALSLGLGLEENEVKEALGGEELIYLLKINYYPPCPRPDLALGVVAHTDMSGITILVPNDVQGLQVFRDGHWYDVEYIPNALIIHIGDQLEVVSNGKYKAVLHRSTVNKEKTRISWPVFVEPPEDKEVGPHPKLVTNENPAKYNTKTYREYKHRKLNKLPQ
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University