Enzyme
Isolated from the plants Silene dioica (red campion) [1], Iris ensata (Japanese iris) [2] and Iris hollandica (Dutch iris) [3]. Also acts on the 3-O-rutinosides of pelargonidin, delphinidin and malvidin, but not the corresponding glucosides or 6-acylglucosides. The enzyme does not catalyse the glucosylation of the 5-hydroxy group of cyanidin 3-glucoside.
5GT
Enzyme
Cyanidin 3-O-rutinoside 5-O-glucosyltransferase
Enzyme name
EC:2.4.1.116
Entry
Transferases;Glycosyltransferases;Hexosyltransferases
Class
UDP-alpha-D-glucose:cyanidin-3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside 5-O-beta-D-glucosyltransferase
Sysname
UDP-alpha-D-glucose + cyanidin-3-O-rutinoside = UDP + cyanidin 3-O-rutinoside 5-O-beta-D-glucoside [RN:R06819]
Reaction
UDP [CPD:C00015];cyanidin 3-O-rutinoside 5-O-beta-D-glucoside [CPD:C12646]
Product
Species information
Hordeum vulgare
Species name
Barley (Hordeum vulgare) is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Hordeum_vulgare.5GT.fa
Download
>HORVU2Hr1G108330.2.r1
QILPSFLLFVSMAKDAQDQRHSRNGGVHTGHHFLIVAYGIQSHLNPCRVLAHRLARLHDGSGPVLATVSLPVSAHRRMFPSSRAVDNDEDTATDGVVSYAPYSDGLDDGSMARDGEARARSRHATFESLSAVVATLAARGRPVTCVVCSMVLPAALDVAREHAIPLAVFWLQPATVLAAYYHYFHGHGDLVASRAADPAYEVSLPGLHRPLRIRDFPSFLVDTTGSMLAKIFNEAARELFEHLGDHGRTKTKVLVNTLDELEPAALAAMKEHLDVFAVGPVIGSSSSAEARIHLFNHAGADEKRYMEWLGAQAARSVVYVSFGSVWTYSEKQMEEIADGLRRCGRPYLLVLRNDGRQEDVSRSLDDAVLQGQGMVVEWCDQPKVLSHPSVGCFVTHCGWNSALEAMALAVPVVAVPGLFDQPTNAFLIEEEWAAGVRGERNSEGVFTGEELARCVELLMGYGPTAIEVRERVEALKGVAQEVVALGGPAERILRSFVQEVGSNAG
>HORVU4Hr1G025920.2.r1
AYVMAQPAVRREHCDGDGRRTHFLVAAYGIQGHLNPARALARRLAAIDGARATLSVPLFGHRRMFPFSSSDDQEEVSDGVIHYAPFSDGQDDGSWPTGSGDETKRRRRASCDSLSAVVRRLAAAGRPVTCVVCTLNMPTVVQVAHAHGLPLAVYWIQPATALVAYYHYFHGHGEGGIAAHAADPAYEATLPGLRRPMRMGRDMPSFLSDDATGTGDDLSQMIVRGFREMFEQMDDEEVIMKPCMVLVNTLEALEATALAAIRTYLGDVFAIGVPVVPLLGAGEDQTIHLFAHDEEKRYMAWLDAQPPKSVVYVSSGSLLTYSERQAEEILCGMRRLNRPYLWVVRREGRSPEVDRLLLATSVAVPEGMVVEWCDQVRVLSHPSVACFVTHCGWNSTLEAVACGVPAVAAPSWSDQPVNAHLLEEEWGVGVRAEREADGVLTGAELARCVELAVGSGERAAAIAVNSRTWKERAKEAVAAGGPSERSLRSFVKRVQELEFLRSN
>HORVU6Hr1G037700.1.r1
MGPQHFLVVAFPGQGHINPARALAERLSRAAPGARVTLSAAVSAHRRMFPSLASPDDEVHDGAISYIPYSDGFDHGFSLFAGDGDEVKRYAEVFGRVGRETFSAVVDRLAARGRPVTCVVYAMLMWWAAEVARERGVPRALYWIQPATMLAVYYHYFNGYERLVTEHAAEPGFTLSMPGLPPLAIRDLPSFFTNFTDGRIVAAFGDIRRTFQQLDLDVDGSSRTGGRQAMVLVNTVEELEAGALASVPELDVFPVGPAVVSLFAEGAGGASGTATAVGDLFEHDEKAYMEWLDTKPARSVVYVSFGSMSAVSKRQKDELKRGLAASGWPYLWVVRKNNRDDGFDDVGGVQGMVVGWCDQVQVLSHPAVGCFVTHCGWNSTLESVACGVSVVAVPQWSDQDTNARLVVQWGIGVRSTTDADRVLKAEELARCVEIIMGDTEEGAAIRASSASWKAKLQEAIADGGSSGRNLRTFLDQFPNDA
>HORVU7Hr1G048160.1.r1
MENRGGAPSPHLLFVTSPMQGHINPVRRLAARVAAAGAAVTVSTAVSGHRRMFPSLASPGEEAVDAAGLLHAPFSDGYDDGFDPRVHDIRSYAARAXX
>HORVU7Hr1G085200.1.r1
PGSISWPENHAVPSQNLSSMERSSATPHFLFVTTPMQGHINPARRLAARVMALMPDARVTFSTAIGGHRLMFPSLVTPDEETVDDAGVLYVPYSDGFDEGFRPEVHGRGTYKERARAVGRETLSAVVAGLAVRGRPVTCMVYAFLVGWAAHVARAHGIPAALYWIQPAAVFAVYYHYFHGHDALIASCANDANPDAAVELPGLPPLRSSTLPSVVSITSPEQPHYLLLDMMRDLFLALDEYKPKVLVNTFDALEPDALRAVAEFELVAVGPVVPDEASPSSADLFRRDDGKGYMEWLDTKPARSVVYVSFGSFLSMSKRQEEETRRGLEATGRPYLWVARKGAGECATLDGGDDDGMRAQGVVVEWCDQVKVLSHPAVGCFVTHCGWNSTLESVTSGVPMVAVPQWTDQPTVAWLVEARMGVGVRARADGEGAVDRGELQRCVETILGDGEAASGIRAQADLWRVRAEEAIAGGGTSERNLRAFALGCALT
>HORVU7Hr1G085270.2.r1
MSPQRHFLVLTFPLQGHIAPALRLARRLLAAAPDALVTFSTTEAAHRRMFPAEPDGGVDDCRLELLPFSDGTDAGYDRSSTDPAAFTDYMASFQAAGARSVGELVDALAARGRPVTRVVYTMLLPWAADVAREKGLPSALYWIQPAAVLAVYYHYFHGYAAVVADHRHDPSFVVRFPGLPPQAVRDLPSFITDSTDPSEFFHSVYTTVRDLFESLDRESPRATVLVNTCQELEEGALAAVRAYDALPIGPVLPSGDEAGLFKQDSAKYMEWLDAKPVDSVVYVAFGSLTGMAREQLDELLLGLEESGRPYLFIVRKDIKAELADAVETDAWPKNGVVVEWCDQVRVLSHAAVGCFVTHCGWNSVMESMACGVPMVCVPQMSDQRMNAWLVEFEWRVGARAEVGGDGVLRAVEVRQRLEEVTQDGEAGAGGARGTASEWKRVVAEALGKDGSSDRNLRAFVEGIISSVSL
>HORVU7Hr1G048200.1.r1
MRIHINRFSASEDASILKAHSTQAKERQTKKANIFVNFCFSEVGERKTRGKKVNARDEFATXXXXXXXXXXXXXXXXXXXXXXXXXXXXEVVGTLRNLFLALDEHRPKVLVNTFDALEPDALGAVPGFELVAVGPVVPDDASAPSTTDLSLCXXXXXXXXXXXXXXXXXSVVYVSFGTIFAVSKRQELETLQALKAAGRPYLWVSRKVAEVDGAELDGAGAGGGADGGQGIMVGWCDQVRVLSHPAVGCFVTHCGWNSALESIACGVPVVAVPQWTDQPTVAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRKILCKF
>HORVU7Hr1G101710.1.r1
MAPQHFLVVAFPGQGHINPARALAERLARATPGARVTLSAAVSAHRRMFPSLASPDDEVHDGAISYVPYSDGYDHGFSLFAGDGDEAERYAEAFGRVGRETFSAVLDRLAARGRPVTCVVYAMLMWWAAEVARERGLPRALYWIQPATMLAVYYHYFHGYERTVTEHAAEPGFTVSMPFLPPMEIRDLPSFFTNLTDGRLAAAFGGIRRTFQQLDLDVGSGGSGAGGSRRAMVLVNTVEELESGALASVPELDVFPVGPAVVSLFTEGEGGTSSGTAAAAAVGDLFEHDEQGYMEWLDTKPARSVAYVSFGSMSAVSKRQKDELRRGLAASGQPYLWVVRNNNRDDGFDAAGDVRGMVVGWCDQVRVLSHPAVGCFVTHCGWNSTLEAVACGAPVVAVPQWSDQDTNARLVVQWGVGVRAATDVDRLLVAEELARCLEMIMGDTEEGAAIRASSAAWKAKLRQAIADSGSSGRNLRIFLNQFANDA
>HORVU7Hr1G048170.2.r1
NLSMFVFIKFELDQICIKAFFAYSGSLKISFYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGCETLAGVVARLAERGRPVTCVVYTFFVSWVPEVARASGIPSALFWIQPAAVFAVYYHYFHGQDAVLASCANDPDRDAVVQLPGLPPLKSRALPSVVSLTSPEERGYEVVGTLRNLFLALDEHRPKVLVNTFDALEPDALGAVPGFELVAVGPVVPDDASAPSTTDLSLCDHDVSGYMEWLDTKAARSVVYVSFGTIFAVSKRQELETLQALKAAGRPYLWVGWRRRRRARDHGGVVRPGARAVAPGGGMLRDALRVELGVGEHRVRRACGGGAAVDGPADGGVAGGGVRGRRGARAGGRRGGGGARRAPKVRGDGHGRRRSSIGDPSMRGQMDGAGQGGGRCRRDAGVESPSVPVWFLKNEQTYA
>HORVU0Hr1G017270.1.r1
PAAVLAVYYHYFHGYAAVVADHRHDPSFVVRFPGLPPQEKMATEVYALIKNHFI
>HORVU0Hr1G037190.1.r1
FGSLTGMAREQLDELLLGLEESGRPYLFIVRKDIKAELADAVETDAWPKNGVVVEWCDQVRVLSHAAVGCFVTHCGWNSVMESMACGVPMVCVPQMSDQRMNAWLVEFEWRVGARAEVGGDGVLRAVEVRQRLEEVTQDGEAGAGGARGTASEWKRVVAEALGKDGSSDRNLRAFVEGIISSVSL
QILPSFLLFVSMAKDAQDQRHSRNGGVHTGHHFLIVAYGIQSHLNPCRVLAHRLARLHDGSGPVLATVSLPVSAHRRMFPSSRAVDNDEDTATDGVVSYAPYSDGLDDGSMARDGEARARSRHATFESLSAVVATLAARGRPVTCVVCSMVLPAALDVAREHAIPLAVFWLQPATVLAAYYHYFHGHGDLVASRAADPAYEVSLPGLHRPLRIRDFPSFLVDTTGSMLAKIFNEAARELFEHLGDHGRTKTKVLVNTLDELEPAALAAMKEHLDVFAVGPVIGSSSSAEARIHLFNHAGADEKRYMEWLGAQAARSVVYVSFGSVWTYSEKQMEEIADGLRRCGRPYLLVLRNDGRQEDVSRSLDDAVLQGQGMVVEWCDQPKVLSHPSVGCFVTHCGWNSALEAMALAVPVVAVPGLFDQPTNAFLIEEEWAAGVRGERNSEGVFTGEELARCVELLMGYGPTAIEVRERVEALKGVAQEVVALGGPAERILRSFVQEVGSNAG
>HORVU4Hr1G025920.2.r1
AYVMAQPAVRREHCDGDGRRTHFLVAAYGIQGHLNPARALARRLAAIDGARATLSVPLFGHRRMFPFSSSDDQEEVSDGVIHYAPFSDGQDDGSWPTGSGDETKRRRRASCDSLSAVVRRLAAAGRPVTCVVCTLNMPTVVQVAHAHGLPLAVYWIQPATALVAYYHYFHGHGEGGIAAHAADPAYEATLPGLRRPMRMGRDMPSFLSDDATGTGDDLSQMIVRGFREMFEQMDDEEVIMKPCMVLVNTLEALEATALAAIRTYLGDVFAIGVPVVPLLGAGEDQTIHLFAHDEEKRYMAWLDAQPPKSVVYVSSGSLLTYSERQAEEILCGMRRLNRPYLWVVRREGRSPEVDRLLLATSVAVPEGMVVEWCDQVRVLSHPSVACFVTHCGWNSTLEAVACGVPAVAAPSWSDQPVNAHLLEEEWGVGVRAEREADGVLTGAELARCVELAVGSGERAAAIAVNSRTWKERAKEAVAAGGPSERSLRSFVKRVQELEFLRSN
>HORVU6Hr1G037700.1.r1
MGPQHFLVVAFPGQGHINPARALAERLSRAAPGARVTLSAAVSAHRRMFPSLASPDDEVHDGAISYIPYSDGFDHGFSLFAGDGDEVKRYAEVFGRVGRETFSAVVDRLAARGRPVTCVVYAMLMWWAAEVARERGVPRALYWIQPATMLAVYYHYFNGYERLVTEHAAEPGFTLSMPGLPPLAIRDLPSFFTNFTDGRIVAAFGDIRRTFQQLDLDVDGSSRTGGRQAMVLVNTVEELEAGALASVPELDVFPVGPAVVSLFAEGAGGASGTATAVGDLFEHDEKAYMEWLDTKPARSVVYVSFGSMSAVSKRQKDELKRGLAASGWPYLWVVRKNNRDDGFDDVGGVQGMVVGWCDQVQVLSHPAVGCFVTHCGWNSTLESVACGVSVVAVPQWSDQDTNARLVVQWGIGVRSTTDADRVLKAEELARCVEIIMGDTEEGAAIRASSASWKAKLQEAIADGGSSGRNLRTFLDQFPNDA
>HORVU7Hr1G048160.1.r1
MENRGGAPSPHLLFVTSPMQGHINPVRRLAARVAAAGAAVTVSTAVSGHRRMFPSLASPGEEAVDAAGLLHAPFSDGYDDGFDPRVHDIRSYAARAXX
>HORVU7Hr1G085200.1.r1
PGSISWPENHAVPSQNLSSMERSSATPHFLFVTTPMQGHINPARRLAARVMALMPDARVTFSTAIGGHRLMFPSLVTPDEETVDDAGVLYVPYSDGFDEGFRPEVHGRGTYKERARAVGRETLSAVVAGLAVRGRPVTCMVYAFLVGWAAHVARAHGIPAALYWIQPAAVFAVYYHYFHGHDALIASCANDANPDAAVELPGLPPLRSSTLPSVVSITSPEQPHYLLLDMMRDLFLALDEYKPKVLVNTFDALEPDALRAVAEFELVAVGPVVPDEASPSSADLFRRDDGKGYMEWLDTKPARSVVYVSFGSFLSMSKRQEEETRRGLEATGRPYLWVARKGAGECATLDGGDDDGMRAQGVVVEWCDQVKVLSHPAVGCFVTHCGWNSTLESVTSGVPMVAVPQWTDQPTVAWLVEARMGVGVRARADGEGAVDRGELQRCVETILGDGEAASGIRAQADLWRVRAEEAIAGGGTSERNLRAFALGCALT
>HORVU7Hr1G085270.2.r1
MSPQRHFLVLTFPLQGHIAPALRLARRLLAAAPDALVTFSTTEAAHRRMFPAEPDGGVDDCRLELLPFSDGTDAGYDRSSTDPAAFTDYMASFQAAGARSVGELVDALAARGRPVTRVVYTMLLPWAADVAREKGLPSALYWIQPAAVLAVYYHYFHGYAAVVADHRHDPSFVVRFPGLPPQAVRDLPSFITDSTDPSEFFHSVYTTVRDLFESLDRESPRATVLVNTCQELEEGALAAVRAYDALPIGPVLPSGDEAGLFKQDSAKYMEWLDAKPVDSVVYVAFGSLTGMAREQLDELLLGLEESGRPYLFIVRKDIKAELADAVETDAWPKNGVVVEWCDQVRVLSHAAVGCFVTHCGWNSVMESMACGVPMVCVPQMSDQRMNAWLVEFEWRVGARAEVGGDGVLRAVEVRQRLEEVTQDGEAGAGGARGTASEWKRVVAEALGKDGSSDRNLRAFVEGIISSVSL
>HORVU7Hr1G048200.1.r1
MRIHINRFSASEDASILKAHSTQAKERQTKKANIFVNFCFSEVGERKTRGKKVNARDEFATXXXXXXXXXXXXXXXXXXXXXXXXXXXXEVVGTLRNLFLALDEHRPKVLVNTFDALEPDALGAVPGFELVAVGPVVPDDASAPSTTDLSLCXXXXXXXXXXXXXXXXXSVVYVSFGTIFAVSKRQELETLQALKAAGRPYLWVSRKVAEVDGAELDGAGAGGGADGGQGIMVGWCDQVRVLSHPAVGCFVTHCGWNSALESIACGVPVVAVPQWTDQPTVAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRKILCKF
>HORVU7Hr1G101710.1.r1
MAPQHFLVVAFPGQGHINPARALAERLARATPGARVTLSAAVSAHRRMFPSLASPDDEVHDGAISYVPYSDGYDHGFSLFAGDGDEAERYAEAFGRVGRETFSAVLDRLAARGRPVTCVVYAMLMWWAAEVARERGLPRALYWIQPATMLAVYYHYFHGYERTVTEHAAEPGFTVSMPFLPPMEIRDLPSFFTNLTDGRLAAAFGGIRRTFQQLDLDVGSGGSGAGGSRRAMVLVNTVEELESGALASVPELDVFPVGPAVVSLFTEGEGGTSSGTAAAAAVGDLFEHDEQGYMEWLDTKPARSVAYVSFGSMSAVSKRQKDELRRGLAASGQPYLWVVRNNNRDDGFDAAGDVRGMVVGWCDQVRVLSHPAVGCFVTHCGWNSTLEAVACGAPVVAVPQWSDQDTNARLVVQWGVGVRAATDVDRLLVAEELARCLEMIMGDTEEGAAIRASSAAWKAKLRQAIADSGSSGRNLRIFLNQFANDA
>HORVU7Hr1G048170.2.r1
NLSMFVFIKFELDQICIKAFFAYSGSLKISFYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGCETLAGVVARLAERGRPVTCVVYTFFVSWVPEVARASGIPSALFWIQPAAVFAVYYHYFHGQDAVLASCANDPDRDAVVQLPGLPPLKSRALPSVVSLTSPEERGYEVVGTLRNLFLALDEHRPKVLVNTFDALEPDALGAVPGFELVAVGPVVPDDASAPSTTDLSLCDHDVSGYMEWLDTKAARSVVYVSFGTIFAVSKRQELETLQALKAAGRPYLWVGWRRRRRARDHGGVVRPGARAVAPGGGMLRDALRVELGVGEHRVRRACGGGAAVDGPADGGVAGGGVRGRRGARAGGRRGGGGARRAPKVRGDGHGRRRSSIGDPSMRGQMDGAGQGGGRCRRDAGVESPSVPVWFLKNEQTYA
>HORVU0Hr1G017270.1.r1
PAAVLAVYYHYFHGYAAVVADHRHDPSFVVRFPGLPPQEKMATEVYALIKNHFI
>HORVU0Hr1G037190.1.r1
FGSLTGMAREQLDELLLGLEESGRPYLFIVRKDIKAELADAVETDAWPKNGVVVEWCDQVRVLSHAAVGCFVTHCGWNSVMESMACGVPMVCVPQMSDQRMNAWLVEFEWRVGARAEVGGDGVLRAVEVRQRLEEVTQDGEAGAGGARGTASEWKRVVAEALGKDGSSDRNLRAFVEGIISSVSL
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University