Enzyme
Isolated from the plant Gentiana triflora. Transfers the hydroxycinnamoyl group only to the C-5 glucoside of anthocyanin. Caffeoyl-CoA, but not malonyl-CoA, can substitute as an acyl donor.
5AT
Enzyme
Anthocyanin 5-aromatic acyltransferase
Enzyme name
EC:2.3.1.153
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanidin 3,5-di-O-beta-D-glucoside 5-O-glucoside-6'''-O-4-hydroxycinnamoyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3,5-di-O-beta-D-glucoside = CoA + anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [RN:R05163]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [CPD:C06363]
Product
Species information
Hamamelis virginiana
Species name
American witch-hazel (Hamamelis virginiana) is a species of eudicot in the family Hamamelidaceae (witch-hazel family).
Species info
Gene sequence info
Hamamelis_virginiana.5AT.fa
Download
>g8203.t1
MEVEVISKECIKPSSPTPNHLRNFKLSLLDQLISAPYAPMVFFYPMNNHDTSSHMIKLCMRMEMLKKSLSETLTPFYPLAGKIKDDLSIECNDEGAYFLEARVNCGLSEFLSQPDILLIHRFLPCEPILKESSAGSFVTNIQANVFECGGIAIGVCISHKILDGAALSTFFKAWAAAARGSYEEAVLYNPDYIAASLFPTNDLWLKDSSMVIFGCLFKKGKSVTSRYVFTASAISALKAKAASSCGGQCPTRVEAVSAFLWKCTMAATKQIHGSPRPSLLTHVVNLRRKKTVLPISENSIGNLIWIATAKCMGKNNQELQGLVGEVREAISKIDGEFVEKLRDDKGYSVICESLKEMRELGCQPEVDCFGFTSWCNFGLYEADFGWGKPVWVSSVGSCGPVFMNLIILIETRSGDGIEAWVTLDEQEMAVLERDLELLEFASLDPSPLN
>g90893.t1
MEWLWQQKPLEAKLRSWRVEPSALQGAENWMSNLKEWLGLWHLVSVAGSPKFCLYVTDFGWGRPKKIEVVHFDASGPISVTECRDEEGGIEVGVALGKVQMEAFSYIFEEGRKVL
>g115660.t1
MSSSSTLLTSPTLVSECTVFPKHKSSIGNLKLSVSDLPMLSCHYIQKGCLFTRPPFPIDTLINILKHGLSQTLTHFPALAGRLITDSDGYVYITCNDAGVDFVGFNATHIFIRDILSPTDVPDLFKEFFAFDRTVSYDGHFKPLMAIQVTELADGVFIGCSVNHSVIDGTSLWNFFNSFAEVSRGASKISRTPDFHRNSVLVSPAVLRFPSGGPMVTFSGDAPLRERIFSFSREAILKMKSKVNNDRKWNGNENPDAVEILGKQSNDTWKNTIKENQNPSGKVTSILENWFRNSITKVPQSTNPTVEISSFQSLCALLWRSVTRARQLPSSKTTTFQMAVNVRHRLEPKLDAYYFGNAIQSIPTYALVGDVLSRDLRWCAEQINSNLKAHDDAAVRRFVEDWERNPQCYPLGNFDGAMMTMGSSFRFPMYDNDFGWGRPIAIRSGPANKFDGKISAFPGRKGGGSVDLEVILSPETMAGLESDSEFMQYVSLAF
>g59509.t1
MPVTFTLVPQILPKVLTSSNDTLVAPLLALKVTLFPKSGTCVGYTFRHAMGDGTTFTHFLKSWASINKLGGDEALIANGSKSLPFYDRTVIKDPNGIETIFWSQVGKKKFQEPQPARNFATNKVRATFVMRQADVQNLKKWVSIQYPTFEPMM
>g81573.t1
MGNRLSHCFASAKTTQLIGENGFLFAAEVIGKEIRERLHNEEGVLKGAEKWISDMEALKRERIKRERIFGVVGSPKFSFYDIDFRWGKKKKSKIVSIDITGNMSLTDCRDVEGVLDVGLSLPKNMMEAFAAIFADGLLKVSNIMCK
>g1224.t1
MQPPLPQWYFGNALFGTSATMNAEDLLSKPLAYASSRIREAIDKGTDEYIRSSYTYVKSQADVSKFRDSFTLGCTQSGFFGNPNLKITSWVNLPVYGADFGWGKEIHMGPGAIGYDGQCFLLPGHDEDGSFIVALRLQVEHMEAFKKFFYEDTLLQKL
>g61448.t1
MGSIRFITTSTVRPASHNESTHRIELTPWDLQLLFVESIQKGLLFHKPTLPKEKALENSLIHNLKTSLSRTLDFFSPLAGRLATIKHDDNTTSFFIECNNTGAEFIHAVADGVTITDIINSVYNIPRNIVSSFFPLNGVLNLEGTSKPLLAVQVTELVDGIFIGCTINHMVADGASFWHFFNSWAEISRGFDHVSNPPILQRWFIDGSDCPIRLHLSQDKQSFKTSSIN
>g83814.t1
MPLPSLFPTWYEAVAGHMWRCASKACRHESQQLTRLRIIMNIQNRMQPPLPQWYFRNALFLKSAMMNTEDLLSKPLTYASSRIREAVDKGTDEYIGSSYTYVKSQANVSKLRNSHTLGCTQSGFFGNPNFEITSWVNLPLYGVDFGWGKEIHMGPGAIGFDGKCFLLPRHDEDGSFIAALRLQVEHMEAFKKFFYEDTLLQKL
>g44407.t1
MPSSDSCFLPFPTLVSKCTVFPPHKSTMADLKLSVSDLPMLSCHYIQKGCLFTRPPLPIDALLNLLKQSLSQTLTHFPALAGRFITDSNGVVYITCNDAGVDFIHANASHIFIRDTLSPIDVPDLFKEFFAFDRTVSYEGHFKPIMAVQVTELADGVFIGCAVNHAVTDGTSFWNFFNTFADVCRGVKRILRMPDFRRNSVLISPVVLRVPPGGPTVTFSGDAPLRERIFSFSREAILKMKSKANNRKWSNGNLDAGEILGKQSNDTWKVTVGANQNSTGKVTSILENWLRSAVSKPQSTETESTDATVEISSFQSLCALLWRSVTRARKLPSSKTTTFRMAINVRHRLEPKLDPYYFGNAIQSIPTYASAGDVLSRDLRWCAEQLNRNVMAHDDATVRRYVEDWEANPRCFPLGNSDGAMMTMGSSPRFPMYDNDFGWGRPMAIRSGRANKFDGKISAFPGREGRGSVDLEVVLSPETMAGLESDSEFMQYVSSS
>g84384.t1
MMKVKKIEIITRETIKPSSPTPHHLKSFKLSFMDQLAPPIYCPLVLFYANIDGDGSSSNHTMMMNSTEISQHLKKSLSETLVHFYPFAGRIEDNFSINCNDDGAEYLEARINCRLSDILGQIEAEALHLFLPNFESIEAIKRPLLFVQATFFECGGMAIGVLLSHKLSDAATLSTFIKAWVAMAVESWTERVVPEFNAESLFPSRDSSIQVPTVKLTKSKCMTRRYVFTASKIASLKAAAASKSVLQPTRVEVVSALIWKCAVQASRSNNKAIKLSVMSQSVNIRKRIVPPLPETSVGNIVGSFVIKRRASDEIELQDLVGELRKGLAEFSDNYVKKLLGDDGFSATCERLEETLNSGGTREGVGVEFFGFTSWCGFRFYEADFGWGKPIWVGIPSSAFKNFALLMDTRDEEGIEAWVFLSEEDMALFECDQELLTFATLNPTVFNKEFGS
>g115082.t1
MTRRYVFTASKIASLKAAAASKSVLQPTRVEVVSALIWKCAVKASRSNNKVIKLSVMSQSVNIRKRIVPPLPETSVGNIVGSFVIKKRASDEIELQDLVGELRKGLAEFSDNYVKKLVGEDGFSATCERLEETLHSGGTREGVGVEFFGFTSWCGFQFYEADFGWGKPIWVGLPSSAIKNFAVLMDTRDGEGIEAWVFLSEEDMALFECDQELLTFATLNPTVFNKEFGS
>g115086.t1
MMKLIKVEVIKRETIKPSSSTPHHLRCFKLSFLDQLAPAVYSSLVLFYSTPIDGDHTMKSTEKSRCLMKSLSDTLIHFYPLAGRIKDSASIDCNDDGAEYLEARVNCSLSDILKQPEAEIQHLFLPGIEPSSAALLRPLLLVQANFFECGGLAIGVCISHKLTDAATFGTFIKAWAANAVESKDRVLRPELINVASLFPPRDLPISLPTVNLTNTNCISRRYVFDASKIATLKAKVASASTSVQQPTRVEVVSALIWKCAMKASRSNSKLIRVSVMSQAVNMRKRIVPPLPENSFGNLNGSFAIQIGEESEIELQSLVSEIRKGIAEFSDNYVKKLQGDKGFLVICEYLEEAGNLLKREDVDFYAFTSWCRFPFYEANFGWGKPIWVGIASWAYKNFVVLMDTRDGEGIEAWVFLSEEDMGLFQHDHELLTFASLNPSVLNMAFES
MEVEVISKECIKPSSPTPNHLRNFKLSLLDQLISAPYAPMVFFYPMNNHDTSSHMIKLCMRMEMLKKSLSETLTPFYPLAGKIKDDLSIECNDEGAYFLEARVNCGLSEFLSQPDILLIHRFLPCEPILKESSAGSFVTNIQANVFECGGIAIGVCISHKILDGAALSTFFKAWAAAARGSYEEAVLYNPDYIAASLFPTNDLWLKDSSMVIFGCLFKKGKSVTSRYVFTASAISALKAKAASSCGGQCPTRVEAVSAFLWKCTMAATKQIHGSPRPSLLTHVVNLRRKKTVLPISENSIGNLIWIATAKCMGKNNQELQGLVGEVREAISKIDGEFVEKLRDDKGYSVICESLKEMRELGCQPEVDCFGFTSWCNFGLYEADFGWGKPVWVSSVGSCGPVFMNLIILIETRSGDGIEAWVTLDEQEMAVLERDLELLEFASLDPSPLN
>g90893.t1
MEWLWQQKPLEAKLRSWRVEPSALQGAENWMSNLKEWLGLWHLVSVAGSPKFCLYVTDFGWGRPKKIEVVHFDASGPISVTECRDEEGGIEVGVALGKVQMEAFSYIFEEGRKVL
>g115660.t1
MSSSSTLLTSPTLVSECTVFPKHKSSIGNLKLSVSDLPMLSCHYIQKGCLFTRPPFPIDTLINILKHGLSQTLTHFPALAGRLITDSDGYVYITCNDAGVDFVGFNATHIFIRDILSPTDVPDLFKEFFAFDRTVSYDGHFKPLMAIQVTELADGVFIGCSVNHSVIDGTSLWNFFNSFAEVSRGASKISRTPDFHRNSVLVSPAVLRFPSGGPMVTFSGDAPLRERIFSFSREAILKMKSKVNNDRKWNGNENPDAVEILGKQSNDTWKNTIKENQNPSGKVTSILENWFRNSITKVPQSTNPTVEISSFQSLCALLWRSVTRARQLPSSKTTTFQMAVNVRHRLEPKLDAYYFGNAIQSIPTYALVGDVLSRDLRWCAEQINSNLKAHDDAAVRRFVEDWERNPQCYPLGNFDGAMMTMGSSFRFPMYDNDFGWGRPIAIRSGPANKFDGKISAFPGRKGGGSVDLEVILSPETMAGLESDSEFMQYVSLAF
>g59509.t1
MPVTFTLVPQILPKVLTSSNDTLVAPLLALKVTLFPKSGTCVGYTFRHAMGDGTTFTHFLKSWASINKLGGDEALIANGSKSLPFYDRTVIKDPNGIETIFWSQVGKKKFQEPQPARNFATNKVRATFVMRQADVQNLKKWVSIQYPTFEPMM
>g81573.t1
MGNRLSHCFASAKTTQLIGENGFLFAAEVIGKEIRERLHNEEGVLKGAEKWISDMEALKRERIKRERIFGVVGSPKFSFYDIDFRWGKKKKSKIVSIDITGNMSLTDCRDVEGVLDVGLSLPKNMMEAFAAIFADGLLKVSNIMCK
>g1224.t1
MQPPLPQWYFGNALFGTSATMNAEDLLSKPLAYASSRIREAIDKGTDEYIRSSYTYVKSQADVSKFRDSFTLGCTQSGFFGNPNLKITSWVNLPVYGADFGWGKEIHMGPGAIGYDGQCFLLPGHDEDGSFIVALRLQVEHMEAFKKFFYEDTLLQKL
>g61448.t1
MGSIRFITTSTVRPASHNESTHRIELTPWDLQLLFVESIQKGLLFHKPTLPKEKALENSLIHNLKTSLSRTLDFFSPLAGRLATIKHDDNTTSFFIECNNTGAEFIHAVADGVTITDIINSVYNIPRNIVSSFFPLNGVLNLEGTSKPLLAVQVTELVDGIFIGCTINHMVADGASFWHFFNSWAEISRGFDHVSNPPILQRWFIDGSDCPIRLHLSQDKQSFKTSSIN
>g83814.t1
MPLPSLFPTWYEAVAGHMWRCASKACRHESQQLTRLRIIMNIQNRMQPPLPQWYFRNALFLKSAMMNTEDLLSKPLTYASSRIREAVDKGTDEYIGSSYTYVKSQANVSKLRNSHTLGCTQSGFFGNPNFEITSWVNLPLYGVDFGWGKEIHMGPGAIGFDGKCFLLPRHDEDGSFIAALRLQVEHMEAFKKFFYEDTLLQKL
>g44407.t1
MPSSDSCFLPFPTLVSKCTVFPPHKSTMADLKLSVSDLPMLSCHYIQKGCLFTRPPLPIDALLNLLKQSLSQTLTHFPALAGRFITDSNGVVYITCNDAGVDFIHANASHIFIRDTLSPIDVPDLFKEFFAFDRTVSYEGHFKPIMAVQVTELADGVFIGCAVNHAVTDGTSFWNFFNTFADVCRGVKRILRMPDFRRNSVLISPVVLRVPPGGPTVTFSGDAPLRERIFSFSREAILKMKSKANNRKWSNGNLDAGEILGKQSNDTWKVTVGANQNSTGKVTSILENWLRSAVSKPQSTETESTDATVEISSFQSLCALLWRSVTRARKLPSSKTTTFRMAINVRHRLEPKLDPYYFGNAIQSIPTYASAGDVLSRDLRWCAEQLNRNVMAHDDATVRRYVEDWEANPRCFPLGNSDGAMMTMGSSPRFPMYDNDFGWGRPMAIRSGRANKFDGKISAFPGREGRGSVDLEVVLSPETMAGLESDSEFMQYVSSS
>g84384.t1
MMKVKKIEIITRETIKPSSPTPHHLKSFKLSFMDQLAPPIYCPLVLFYANIDGDGSSSNHTMMMNSTEISQHLKKSLSETLVHFYPFAGRIEDNFSINCNDDGAEYLEARINCRLSDILGQIEAEALHLFLPNFESIEAIKRPLLFVQATFFECGGMAIGVLLSHKLSDAATLSTFIKAWVAMAVESWTERVVPEFNAESLFPSRDSSIQVPTVKLTKSKCMTRRYVFTASKIASLKAAAASKSVLQPTRVEVVSALIWKCAVQASRSNNKAIKLSVMSQSVNIRKRIVPPLPETSVGNIVGSFVIKRRASDEIELQDLVGELRKGLAEFSDNYVKKLLGDDGFSATCERLEETLNSGGTREGVGVEFFGFTSWCGFRFYEADFGWGKPIWVGIPSSAFKNFALLMDTRDEEGIEAWVFLSEEDMALFECDQELLTFATLNPTVFNKEFGS
>g115082.t1
MTRRYVFTASKIASLKAAAASKSVLQPTRVEVVSALIWKCAVKASRSNNKVIKLSVMSQSVNIRKRIVPPLPETSVGNIVGSFVIKKRASDEIELQDLVGELRKGLAEFSDNYVKKLVGEDGFSATCERLEETLHSGGTREGVGVEFFGFTSWCGFQFYEADFGWGKPIWVGLPSSAIKNFAVLMDTRDGEGIEAWVFLSEEDMALFECDQELLTFATLNPTVFNKEFGS
>g115086.t1
MMKLIKVEVIKRETIKPSSSTPHHLRCFKLSFLDQLAPAVYSSLVLFYSTPIDGDHTMKSTEKSRCLMKSLSDTLIHFYPLAGRIKDSASIDCNDDGAEYLEARVNCSLSDILKQPEAEIQHLFLPGIEPSSAALLRPLLLVQANFFECGGLAIGVCISHKLTDAATFGTFIKAWAANAVESKDRVLRPELINVASLFPPRDLPISLPTVNLTNTNCISRRYVFDASKIATLKAKVASASTSVQQPTRVEVVSALIWKCAMKASRSNSKLIRVSVMSQAVNMRKRIVPPLPENSFGNLNGSFAIQIGEESEIELQSLVSEIRKGIAEFSDNYVKKLQGDKGFLVICEYLEEAGNLLKREDVDFYAFTSWCRFPFYEANFGWGKPIWVGIASWAYKNFVVLMDTRDGEGIEAWVFLSEEDMGLFQHDHELLTFASLNPSVLNMAFES
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University