Enzyme
One of three specific glucosyltransferases in pea (Pisum sativum) that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase). TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly.
UGT79B30
Enzyme
UDP-glycosyltransferase 79B30
Enzyme name
EC:2.4.1.239
Entry
Transferases;Glycosyltransferases;Hexosyltransferases
Class
UDP-glucose:flavonol-3-O-beta-D-glucoside 2''-O-beta-D-glucosyltransferase
Sysname
UDP-glucose + a flavonol 3-O-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside [RN:R07339]
Reaction
UDP [CPD:C00015];flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside [CPD:C15581]
Product
Species information
Hamamelis virginiana
Species name
American witch-hazel (Hamamelis virginiana) is a species of eudicot in the family Hamamelidaceae (witch-hazel family).
Species info
Gene sequence info
Hamamelis_virginiana.UGT79B30.fa
Download
>g53764.t1
MGGKTFHIAMYPWFAMGHHIPFLHISNKLAERGHRISFFLPTKTQLKLEPFNLHPHLITFIPITVPHVDGLPPGAETTSDVPSSLHPLIMTAMDLTKPAIEASLRDLKPQFIFFDFTHWLPLLAHGLGIKSIHYTIVNSATVAYLIRPERKLNERQLITADALMMMLMMEPPQGFPSSSIKLHAHEARELAAMAVREFGSGIPFMQRQFDSRSNCDAMTFKTCREIEGPYIDYLEKQFKKPVILAGPVVPERPTSALEERWAEWLGRFKAGTVIYCAFGTECILKKDQFQELVLGFELTGMPFFAALKPPLEGETIELALPEDFTQRTQGRGIVHGGWVQQQLILGHPSVGCFVTHCGSGSLLEAMVNKCQLVLLPHVLDHIFNARMMSRDLKIGVEVEKGEEDGLFTRDGVCKAVRSVMDNDSEVGKEVSINHAKWREFLLRKGLESSYIDSFVQKLQDLLG
>g53765.t1
MGGETFHIAMYPWFAMGHLTSFLHISNKLAERGHKISFFLPTKTQPKLEPFNLHPHLINFIPVTVPHVDGLPPGTETTSDVPSSLHGLIVTAMDLTEPAIEASLRDLKPQFIFFDFTHWLPALTSRLGIKSICYCIVSSGSVGYLMSPERKLNGKQVTAAILMEPPQGFPSSSIKLHKHEAQALAAFITVEEYRSGISFMDRLLMSFSGCDAIGFKTCREIEGPYIEYIEKQFKKPVILAGPVVPLRPTSALEERWAKWLGRFKPGTVIFCAFGSECILKKDQFQELVLGFELTGLPFFAALKPPAEAEAIELALPEGFVERTQGRGIVHGGWVQQQLILGHPSIGCFVTHCGSGSLLEAMVNECQLVLLPHVGDQIINARMMGGDLKIGIEVEKVEEDGLFTRDGVGKAVKFVMDDESEVGKEVRTNHAKWRELLLREGLGSSYIDSFVQKLQGLLG
>g118663.t2
MGASLLLTAFQIWLSSCLSLSSEDLRSSTWYLCTLMVINVINDHSLQAAENVGDGTSCLLAGRDIVLEKGQFQELHLGFELTQLPILVALQPPQGCATIEEALPENFLKRVQGRALVYGETLVNDCQIVLLPFLGDQIINTRIMVEELKVAV
>g133067.t1
MEDTATEIEIEAHVSIDMENNSITNQKIEKEEDDDDNDLIFKDSPLFASIKAMLETLSPQPSECSSIFVLDGFFNSVLFDEEPQLLELVHKCCRNINQLPHRITTVSTRELKHLVDYIHHVYVSSIPELRLVPSKSKKSHFVDFLRRCYLRLSFRKVEQKQRGEYKRSPTVTQLHQAGIKFKKGTSISLFDIKFSNNGILEIPQLTIDDLTGSWFQNLIAQGTVLLETQAVAGPLDELWAKWLNKFKPGSVVFCALGSQIVVEKEGCAMVEQGLPKGILERAEGRGWVPQSAIVKHPSIGCFVSQCGFGSIWESLVSDCQIGLVPHLIFQIVNTRLMVEELKVAAKVERGENWWFARRT
>g72665.t1
MGGKTFHIAMYPWFAMGHLTSFLHVSNKLAEKGHKISFFLPTKTQPKLELFNLHPHLINFVPVTVPHVDGLPPGTETTSDVPCSLYGLIMIAMDLTEPVIEAYLRDLKPQFIFFDFTHWLPALTRPLGIKSVHYCTISSASVGYLMSPERKLHGKQETPAILMEPPQGFPSSSIKLHKHEAQALAFITAEEYGSG
>g34613.t1
MDRLLISLSGCDAIGFKTCREIEEPYIDYIEKQFKKPVILAGPVVPERPTSALEERWAKWLGRFKPGTVIFCAFGSECILKMDQFQELVLGFELTGLPFFAALKPPAEAETIELALPEGFGERTQGRGIVHGGWVQQQLILGHPSVGCFMTHCGSGSLLEAMVNECQLVLLPHVGDQIINARMMSGDLKIGIEVEKGEEDGLFTRDGVCKAVKFVMDNDSEVGKEVRTNHAKWRELLLREGLESSYIDSFVQKLQGLLG
>g69405.t1
MASLKCPKLHFAMFPWFAYGHMTPYLHLSNKLAERGHTVSFLLPKGTQPRMEQLSLNQELIHFFPLHIPHLHGLPPGAETASDVPILNHSLLATAFDQTQDQLETILSSHKPDFIFFYFGFWVPALARHLGIKSIYYSVTPPGYPSSKVKLRPHEASKSQLLAQELGSGLTLYERLTTGMKECDAIAMRTFAEIEGPFCDYISQQYAKPVLLTGTVLPEIQAIVVEKGQFQELVQGFELAQLPFLVALQPPEGCATVEEGLPEGFLERVEGRGWVYGGWVPQPEILKHPSIGCFVSHCGFGSMWESLVNECQIVLAPHLVDQIVNTRLMVEELEVVVEVERGENGWFSKEKLSQAIKSVMDDSQLVAAHIRNDHAKWKDIILSHDLDGRYIHSFIQNMQNLLVPGIKDMH
MGGKTFHIAMYPWFAMGHHIPFLHISNKLAERGHRISFFLPTKTQLKLEPFNLHPHLITFIPITVPHVDGLPPGAETTSDVPSSLHPLIMTAMDLTKPAIEASLRDLKPQFIFFDFTHWLPLLAHGLGIKSIHYTIVNSATVAYLIRPERKLNERQLITADALMMMLMMEPPQGFPSSSIKLHAHEARELAAMAVREFGSGIPFMQRQFDSRSNCDAMTFKTCREIEGPYIDYLEKQFKKPVILAGPVVPERPTSALEERWAEWLGRFKAGTVIYCAFGTECILKKDQFQELVLGFELTGMPFFAALKPPLEGETIELALPEDFTQRTQGRGIVHGGWVQQQLILGHPSVGCFVTHCGSGSLLEAMVNKCQLVLLPHVLDHIFNARMMSRDLKIGVEVEKGEEDGLFTRDGVCKAVRSVMDNDSEVGKEVSINHAKWREFLLRKGLESSYIDSFVQKLQDLLG
>g53765.t1
MGGETFHIAMYPWFAMGHLTSFLHISNKLAERGHKISFFLPTKTQPKLEPFNLHPHLINFIPVTVPHVDGLPPGTETTSDVPSSLHGLIVTAMDLTEPAIEASLRDLKPQFIFFDFTHWLPALTSRLGIKSICYCIVSSGSVGYLMSPERKLNGKQVTAAILMEPPQGFPSSSIKLHKHEAQALAAFITVEEYRSGISFMDRLLMSFSGCDAIGFKTCREIEGPYIEYIEKQFKKPVILAGPVVPLRPTSALEERWAKWLGRFKPGTVIFCAFGSECILKKDQFQELVLGFELTGLPFFAALKPPAEAEAIELALPEGFVERTQGRGIVHGGWVQQQLILGHPSIGCFVTHCGSGSLLEAMVNECQLVLLPHVGDQIINARMMGGDLKIGIEVEKVEEDGLFTRDGVGKAVKFVMDDESEVGKEVRTNHAKWRELLLREGLGSSYIDSFVQKLQGLLG
>g118663.t2
MGASLLLTAFQIWLSSCLSLSSEDLRSSTWYLCTLMVINVINDHSLQAAENVGDGTSCLLAGRDIVLEKGQFQELHLGFELTQLPILVALQPPQGCATIEEALPENFLKRVQGRALVYGETLVNDCQIVLLPFLGDQIINTRIMVEELKVAV
>g133067.t1
MEDTATEIEIEAHVSIDMENNSITNQKIEKEEDDDDNDLIFKDSPLFASIKAMLETLSPQPSECSSIFVLDGFFNSVLFDEEPQLLELVHKCCRNINQLPHRITTVSTRELKHLVDYIHHVYVSSIPELRLVPSKSKKSHFVDFLRRCYLRLSFRKVEQKQRGEYKRSPTVTQLHQAGIKFKKGTSISLFDIKFSNNGILEIPQLTIDDLTGSWFQNLIAQGTVLLETQAVAGPLDELWAKWLNKFKPGSVVFCALGSQIVVEKEGCAMVEQGLPKGILERAEGRGWVPQSAIVKHPSIGCFVSQCGFGSIWESLVSDCQIGLVPHLIFQIVNTRLMVEELKVAAKVERGENWWFARRT
>g72665.t1
MGGKTFHIAMYPWFAMGHLTSFLHVSNKLAEKGHKISFFLPTKTQPKLELFNLHPHLINFVPVTVPHVDGLPPGTETTSDVPCSLYGLIMIAMDLTEPVIEAYLRDLKPQFIFFDFTHWLPALTRPLGIKSVHYCTISSASVGYLMSPERKLHGKQETPAILMEPPQGFPSSSIKLHKHEAQALAFITAEEYGSG
>g34613.t1
MDRLLISLSGCDAIGFKTCREIEEPYIDYIEKQFKKPVILAGPVVPERPTSALEERWAKWLGRFKPGTVIFCAFGSECILKMDQFQELVLGFELTGLPFFAALKPPAEAETIELALPEGFGERTQGRGIVHGGWVQQQLILGHPSVGCFMTHCGSGSLLEAMVNECQLVLLPHVGDQIINARMMSGDLKIGIEVEKGEEDGLFTRDGVCKAVKFVMDNDSEVGKEVRTNHAKWRELLLREGLESSYIDSFVQKLQGLLG
>g69405.t1
MASLKCPKLHFAMFPWFAYGHMTPYLHLSNKLAERGHTVSFLLPKGTQPRMEQLSLNQELIHFFPLHIPHLHGLPPGAETASDVPILNHSLLATAFDQTQDQLETILSSHKPDFIFFYFGFWVPALARHLGIKSIYYSVTPPGYPSSKVKLRPHEASKSQLLAQELGSGLTLYERLTTGMKECDAIAMRTFAEIEGPFCDYISQQYAKPVLLTGTVLPEIQAIVVEKGQFQELVQGFELAQLPFLVALQPPEGCATVEEGLPEGFLERVEGRGWVYGGWVPQPEILKHPSIGCFVSHCGFGSMWESLVNECQIVLAPHLVDQIVNTRLMVEELEVVVEVERGENGWFSKEKLSQAIKSVMDDSQLVAAHIRNDHAKWKDIILSHDLDGRYIHSFIQNMQNLLVPGIKDMH
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University