Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Hamamelis virginiana
Species name
American witch-hazel (Hamamelis virginiana) is a species of eudicot in the family Hamamelidaceae (witch-hazel family).
Species info
Gene sequence info
Hamamelis_virginiana.FLS.fa
Download
>g19756.t1
MEVERVQALAYGGLQQLPPQFIRPAHERPENSKAIEGVTVPVISLSLPHDVLVHQIFKASSEWGFFLITDHGMSPALIGELKKVGEEFFRLPQEEKERYANDHASGKFEGYGTKMTKNHEEKVEWIDYYFHIMSPPSKVNYEMWPQNPPSYRKVTEEYNKEVSKVTDKLLELLSEGLGLEGKVLKSCLGGEKIEAEMKINMYPPCPQPELALGVEPHTDMSALTLLVSNDVVGLQVWKDGNWVAVDYVPSALFVHVGDQIEILSNGKYKSVLHRSLVNKERMRMSWAVFYVPPHETSIGPLPDLVHDQNPAKYSTKTFAEFRYRKFNRLPQ
>g129123.t1
VLSNGKYKSVLHRSLVNKERMRMSWAVFYVPPHEASIGPLPDLVHDQNPAKYSSKTFAEFRYRKFNKLPQ
>g50527.t1
MEVERVQAPAYGGLQQLPQQFIRPAHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLV
>g80257.t1
VLSNGKYKSVLHRSLVNKERMRMSWAVFYIPPHEASIGPLPDLVHDQNPAKYSTKTFAEFRYRKFNKLPQ
>g54873.t1
MPKEFIRSENEQPAITTFHGPVPEVPVIDFSNPDEENIINAMVEASQEWGMFQIVNHGISDELISKLQKVGKEFFELPAEEKEHCAKIPGSKSVEGYGTLLQKEVEGKKGWVDHLFHKIWPPSAINHRFWPQNPPAYREINEEYAKGMHGVVNKLMKNLSLGLGLEGDDVKEAVGGDELVYLLKINYYPPCPRPDLALGVVAHTDMSALTILVPNEVQGLQVFKDDHWFDVEYIPNALIIHIGDQMEIVSNGRFKAVLHRTTVTKDKTRMSWPVFLEPPPELTVGPHPKLVNEENPAKYKTKKYCDYVYCKLNKLPQ
>g40826.t1
MEVERVQALAYGGLQQLPPQFIRPAHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLITDHGISSALIGELREVGEEFFRLPPEEKERYANDHASGKFE
>g32430.t1
MEVERVQALAYGGLQQLPPQFIRPAHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLVTDHGMSSALIGELKEVGEEFFRLPQEEKERYANDHAS
>g117684.t1
MEVERVQALANGGLQQLPPQFIRPAHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLVTDHGMSPALIGELKEVGEDFFRLPQEEKERYANDHASGKFEGYGTKMTKNHEEKVEWIDYYFHIMSPPSKVNYERWPQNPPSY
>g75381.t1
GKSQKNKTKSY*KKQTSSWSCYQRG*D*KEKSRNPIWVARK*KQE*K*TCLQVWKDGNWVAVDYVPSALFVQVGDQIEV
>g21591.t1
MEVERVQALAYGGLQQLPPQFIRPTHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLVTDHGMSPALIGELKEVGEDFFKLPQEEKERYANDHASGKFEGWPQNPPSYRKVTEEYNEELSKVTDKLLELLSEGLGLEGKVLKSCLGGEKIEGEMKINMYPPCPQPELALGVEPHTDMSALTLLVSNDVAGLQVWKDGNWVAVDYVPSALFVHIGDQIEILSNGKYKSVLHRSLVNKERMRMSWAVFYVPPHEASIGPLSDLVHNQNPAKYSTKTYAEFRYRKFNKLPQ
>g26536.t1
MEVERVQALAYGGLQQLPPQFIRPTHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLVTDHGMSPALIGELKEVGEDFFKLPQEEKERYANDHASGKFEGWPQNPPSYRKVTEEYNEELSKVTDKLLELLSEGLGLEGKVLKSCLGGEKIEGEMKINMYPPCPQPELALGVEPHTDMSALTLLVSNDVAGLQVWKDGNWVAVDYVPSALFVHVGDQIEILSNGKYKSVLHRSLVNKERMRMSWAVFYVPPHEASIGPLPDLVHDQNPAKYSTKTYAEFRYRKFNKLPQ
MEVERVQALAYGGLQQLPPQFIRPAHERPENSKAIEGVTVPVISLSLPHDVLVHQIFKASSEWGFFLITDHGMSPALIGELKKVGEEFFRLPQEEKERYANDHASGKFEGYGTKMTKNHEEKVEWIDYYFHIMSPPSKVNYEMWPQNPPSYRKVTEEYNKEVSKVTDKLLELLSEGLGLEGKVLKSCLGGEKIEAEMKINMYPPCPQPELALGVEPHTDMSALTLLVSNDVVGLQVWKDGNWVAVDYVPSALFVHVGDQIEILSNGKYKSVLHRSLVNKERMRMSWAVFYVPPHETSIGPLPDLVHDQNPAKYSTKTFAEFRYRKFNRLPQ
>g129123.t1
VLSNGKYKSVLHRSLVNKERMRMSWAVFYVPPHEASIGPLPDLVHDQNPAKYSSKTFAEFRYRKFNKLPQ
>g50527.t1
MEVERVQAPAYGGLQQLPQQFIRPAHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLV
>g80257.t1
VLSNGKYKSVLHRSLVNKERMRMSWAVFYIPPHEASIGPLPDLVHDQNPAKYSTKTFAEFRYRKFNKLPQ
>g54873.t1
MPKEFIRSENEQPAITTFHGPVPEVPVIDFSNPDEENIINAMVEASQEWGMFQIVNHGISDELISKLQKVGKEFFELPAEEKEHCAKIPGSKSVEGYGTLLQKEVEGKKGWVDHLFHKIWPPSAINHRFWPQNPPAYREINEEYAKGMHGVVNKLMKNLSLGLGLEGDDVKEAVGGDELVYLLKINYYPPCPRPDLALGVVAHTDMSALTILVPNEVQGLQVFKDDHWFDVEYIPNALIIHIGDQMEIVSNGRFKAVLHRTTVTKDKTRMSWPVFLEPPPELTVGPHPKLVNEENPAKYKTKKYCDYVYCKLNKLPQ
>g40826.t1
MEVERVQALAYGGLQQLPPQFIRPAHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLITDHGISSALIGELREVGEEFFRLPPEEKERYANDHASGKFE
>g32430.t1
MEVERVQALAYGGLQQLPPQFIRPAHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLVTDHGMSSALIGELKEVGEEFFRLPQEEKERYANDHAS
>g117684.t1
MEVERVQALANGGLQQLPPQFIRPAHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLVTDHGMSPALIGELKEVGEDFFRLPQEEKERYANDHASGKFEGYGTKMTKNHEEKVEWIDYYFHIMSPPSKVNYERWPQNPPSY
>g75381.t1
GKSQKNKTKSY*KKQTSSWSCYQRG*D*KEKSRNPIWVARK*KQE*K*TCLQVWKDGNWVAVDYVPSALFVQVGDQIEV
>g21591.t1
MEVERVQALAYGGLQQLPPQFIRPTHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLVTDHGMSPALIGELKEVGEDFFKLPQEEKERYANDHASGKFEGWPQNPPSYRKVTEEYNEELSKVTDKLLELLSEGLGLEGKVLKSCLGGEKIEGEMKINMYPPCPQPELALGVEPHTDMSALTLLVSNDVAGLQVWKDGNWVAVDYVPSALFVHIGDQIEILSNGKYKSVLHRSLVNKERMRMSWAVFYVPPHEASIGPLSDLVHNQNPAKYSTKTYAEFRYRKFNKLPQ
>g26536.t1
MEVERVQALAYGGLQQLPPQFIRPTHERPENSKAIEGVTVPVISLSLPHDVLVHEIFKASSEWGFFLVTDHGMSPALIGELKEVGEDFFKLPQEEKERYANDHASGKFEGWPQNPPSYRKVTEEYNEELSKVTDKLLELLSEGLGLEGKVLKSCLGGEKIEGEMKINMYPPCPQPELALGVEPHTDMSALTLLVSNDVAGLQVWKDGNWVAVDYVPSALFVHVGDQIEILSNGKYKSVLHRSLVNKERMRMSWAVFYVPPHEASIGPLPDLVHDQNPAKYSTKTYAEFRYRKFNKLPQ
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University