Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Hamamelis virginiana
Species name
American witch-hazel (Hamamelis virginiana) is a species of eudicot in the family Hamamelidaceae (witch-hazel family).
Species info
Gene sequence info
Hamamelis_virginiana.HI4_OMT.fa
Download
>g53814.t1
MSIKCAVQLGILDIILKQNQPITLPELVSALQVPQEKTGYMHWLMRLLVCSGFFTLRKVLKNHEDDDEEKGYVLTPPSRILLKDDIPSLSPIVLPFLDPALVTPWHFLGDWFLGNKVTAFETAHGMGLWEYEYQNPQLTKLLDEAMASDCRIISSIIGDYGAKFEGLASLVDVGCGTGTVAWTILEAFPPLKCTVLDLLRVIANVPKNKNLDFVGGDMFQSIPFADAILMKMHQSSSHHSHWL
>g53811.t1
MDPAHCQGATEVFQAQFHIYKHVYNYIDSMALKCAVQLGIPDIIHKHNQPITLPELVSALQVLSTKTSYVHRLMRLLVHSGFFATRKVAENEEEEGYVLTPSSRLLLKDNITSLSPAVLAILDPALVTPWHFLGEWFHGSKLTAFETAYGIDFWDYGNQNPEFSKTMNGLMASDSQIISSVIQEYRAVFEGLGSLVDVGGGTGTIARAISEAFPHLKCTVLDLPRVVANLPKSENLDYVGGDMFQSIPSSDAVLIKSVLHNWSDEDCVKILKQCREAISSKNEGGKVIIIDMVIDDNKGDYDVAKTKLFNDLMMMVLVAGRERNEKEWEKLFLEAGFSHYKIMPTSGLMSLIEMGFCVRELKLLKLSQVDTEVEVTATAIQLGIPDVIHKHNQPITLPELVTALQVPSTKTSYVHRLMRLLVHSGFFATRKVHENQEEEGYVLTPSSRLLLKDNITSLSPAVLGFLDPGMVASWHFLGEWFHGSKPTPFETAHGMRFSDYRKQNLEFSKFLMEGTANDSRLISSVIQEYRTIFEGLASLVDLGGGVGTVSRAISEVFPHLKCTVLDLPHVVANLPKTENLDYVGGDMFQPIPSADAVLIKSVLHMFSDEDCVKVLKRCREAIPSKNEGGKVIIIDRMINEKKDEHELTKTKLCNDVMMMIVHAGRDRNEKDWEKLFLEAGFSDYKITIPTLGLLSLIEVYP
>g144755.t1
MEVARESASRGVFGCFWLGFEDRTKTRILSGRMAVFGYWKIEFLTWIGLIVGREGCPADLRLIGGGALWWWQGPNELFQAQSHLYNHVSGYVTSMSIKCAVELGIAEMISNHGQPITLPELVSALPIPPSKATCVHQLMRILVFSGIFATTKVHEKQEEEEEKEWYVLTPSSRLLLKENTTNLLPFVAAIFDPMFITPWHSLGKWFMGNQNTAFETTYGMGFWDYGVKNPEFNNFFNEAMASDSLLMSSAFIDCKSVFAGLGSLIDVGGGTGNTAKII
>g144758.t1
MDLNQSQGAIELGIPDIIFKHGQPITLPELVSALPILSTKATCVHQLMRILVHSGIFASTKVHENQQEKEEEEGYVLTPSSRLLLKDNTTNLSPFVLVILDPTLVTPCHFLGKWFMGNQNTAFETAYGMGFWDYGVQNPKFSNFFNEAMASDSRLMSSVIRECKSVFEGLGSLVDVGGGTGSTAKVICETFPHLKCTVFDLPHVVANLQESANLNYVGGDMFHSIPSADAILLKWILHDWSNEDCVKILKRCREAIRSNEGGKVMIIDTVIDVKNDEHELTETKLSFNMLMMVLFNGKERNEKEWEKLFLDADFSHYKITPIFGFRSLIEVYP
>g134349.t1
MKWILHDWNDEDCVKILKNCRKAIPEKSGKVIIVDVVLQPEGNGVFDDIGLIFDLVMIAHTSGGKERTELEWKKLLEEGGFPRYKIIKIPALQSIIEAYPA
>g58775.t1
MSLKCAVELGISDVIFKHGQPITLPELVSALPIPSTKATCVHQLMRILVHSGIFATTKVHENQQEKEEEEEGYVLTPSSRLLLKDNTTNSLPFVAAILDPTLVTPCHFLGKWFMGNQNTAFETAYGMGFWDYGVQNPKFNNFFNEAMACETRLMSSVIRECKSVFEGLDSLVDVGGGTGSTAKVICEALPHLKCTVFDLPHVVANLPESENLNYVGGDMFHSIPSADAILLQWILHDWSNEDCVKILKRCREAIRSNDEGGKVIIIGIVIDVKNDEHELTETKLSFDMLMMVLFNGKERNEKEWEKLFLDADFSHYKITPIFGFRSLIEVYP
>g41869.t1
MSLKCAVELGISDVIFKHGQPITLPELVSALPIPSTKATCVHQLMRILVHSGIFATTKVHENQQEKEEEEEGYVLTPSSRLLLKDNTTNSLPFVAAILDPTLVTPCHFLGKWFMGNQNTAFETAYGMGFWDYGVQNPKFNNFFNEAMACETRLMSSVIRECKSVFEGLDSLVDVGGGTGSTAKVICEAFPHLKCTVFDLPHVVANLPESENLNYVGGDMFHSIPSADAILLQWILHDWSNEDCVKILKRCREAIRSNDEGGKVIIIGIVIDVKNDEHELTETKLSFDMLMMVLFNGKERNEKEWEKLFLDADFSHYKITPIFGFRSLIEVYP
>g5529.t1
MSVKCAVELGIPDIISKHGQPITLPELVSALPIPSTKATCVHQLMRILVHSGIFATTKVHENQQEEEEEEGYVLTPSSRLLLKDNTANLLPFVAAILDPTFVAPWHFLGKWFMGSQNTAFETAYGMGFWDYGVQNPKFNNLFNEAMASDSRLTSSVIRECKSVFEGLGSLVDVGGGTGSTAKVICEAFPHLKCTVFDLPHVVANLPESQNLNYVGGDMFHSIPSADAVLLKWILHDWSDEDCVKILKQCREAIRSNDEGGKVMIIDVVIDVKNDEHELTETKLSFDMLMMVLFNGKERNEKEWEKLFLAADFSHYKITPIFGFRSLIEVYP
>g47185.t1
MGTLSTWFQNTVDPTSFHTVHGKSFYDFLANEPSYGNMFNECMTNDTQLLTNLMIKECKGVFEGLKSLVDVGGGTGTVAKAIADAFPHSKCTVLDK
>g145521.t1
MSLKCAVQLGIPDIIHNHGKPITLSEVITALQIPPTKTTHMHRLMRILVHSGIFARKKVHENNQEEEEEEEEGYDLTPSSRLLIKDNITSLSPFAVAMLDPVLVTPWHFLGDWFQGNKKKNITAFETAHGMGFWDFMDQNSEFNKIFNEAMASDSGLMSSVIRDCKPVFEGLGSLVDVGGGTGLAAKIILEAFPHLKCTVFDFPHVVANLPESENLDFVGGNMFQSIPSADALLFKLVFHSISDEDCLKILKRCREAIPSKDKKGKVIIIDIVIDVKNDEHKLTETKLSFDMLMMALVTGRERSEKEWEKLFLEAGFSRYKITPMFGLRSLIEVYP
MSIKCAVQLGILDIILKQNQPITLPELVSALQVPQEKTGYMHWLMRLLVCSGFFTLRKVLKNHEDDDEEKGYVLTPPSRILLKDDIPSLSPIVLPFLDPALVTPWHFLGDWFLGNKVTAFETAHGMGLWEYEYQNPQLTKLLDEAMASDCRIISSIIGDYGAKFEGLASLVDVGCGTGTVAWTILEAFPPLKCTVLDLLRVIANVPKNKNLDFVGGDMFQSIPFADAILMKMHQSSSHHSHWL
>g53811.t1
MDPAHCQGATEVFQAQFHIYKHVYNYIDSMALKCAVQLGIPDIIHKHNQPITLPELVSALQVLSTKTSYVHRLMRLLVHSGFFATRKVAENEEEEGYVLTPSSRLLLKDNITSLSPAVLAILDPALVTPWHFLGEWFHGSKLTAFETAYGIDFWDYGNQNPEFSKTMNGLMASDSQIISSVIQEYRAVFEGLGSLVDVGGGTGTIARAISEAFPHLKCTVLDLPRVVANLPKSENLDYVGGDMFQSIPSSDAVLIKSVLHNWSDEDCVKILKQCREAISSKNEGGKVIIIDMVIDDNKGDYDVAKTKLFNDLMMMVLVAGRERNEKEWEKLFLEAGFSHYKIMPTSGLMSLIEMGFCVRELKLLKLSQVDTEVEVTATAIQLGIPDVIHKHNQPITLPELVTALQVPSTKTSYVHRLMRLLVHSGFFATRKVHENQEEEGYVLTPSSRLLLKDNITSLSPAVLGFLDPGMVASWHFLGEWFHGSKPTPFETAHGMRFSDYRKQNLEFSKFLMEGTANDSRLISSVIQEYRTIFEGLASLVDLGGGVGTVSRAISEVFPHLKCTVLDLPHVVANLPKTENLDYVGGDMFQPIPSADAVLIKSVLHMFSDEDCVKVLKRCREAIPSKNEGGKVIIIDRMINEKKDEHELTKTKLCNDVMMMIVHAGRDRNEKDWEKLFLEAGFSDYKITIPTLGLLSLIEVYP
>g144755.t1
MEVARESASRGVFGCFWLGFEDRTKTRILSGRMAVFGYWKIEFLTWIGLIVGREGCPADLRLIGGGALWWWQGPNELFQAQSHLYNHVSGYVTSMSIKCAVELGIAEMISNHGQPITLPELVSALPIPPSKATCVHQLMRILVFSGIFATTKVHEKQEEEEEKEWYVLTPSSRLLLKENTTNLLPFVAAIFDPMFITPWHSLGKWFMGNQNTAFETTYGMGFWDYGVKNPEFNNFFNEAMASDSLLMSSAFIDCKSVFAGLGSLIDVGGGTGNTAKII
>g144758.t1
MDLNQSQGAIELGIPDIIFKHGQPITLPELVSALPILSTKATCVHQLMRILVHSGIFASTKVHENQQEKEEEEGYVLTPSSRLLLKDNTTNLSPFVLVILDPTLVTPCHFLGKWFMGNQNTAFETAYGMGFWDYGVQNPKFSNFFNEAMASDSRLMSSVIRECKSVFEGLGSLVDVGGGTGSTAKVICETFPHLKCTVFDLPHVVANLQESANLNYVGGDMFHSIPSADAILLKWILHDWSNEDCVKILKRCREAIRSNEGGKVMIIDTVIDVKNDEHELTETKLSFNMLMMVLFNGKERNEKEWEKLFLDADFSHYKITPIFGFRSLIEVYP
>g134349.t1
MKWILHDWNDEDCVKILKNCRKAIPEKSGKVIIVDVVLQPEGNGVFDDIGLIFDLVMIAHTSGGKERTELEWKKLLEEGGFPRYKIIKIPALQSIIEAYPA
>g58775.t1
MSLKCAVELGISDVIFKHGQPITLPELVSALPIPSTKATCVHQLMRILVHSGIFATTKVHENQQEKEEEEEGYVLTPSSRLLLKDNTTNSLPFVAAILDPTLVTPCHFLGKWFMGNQNTAFETAYGMGFWDYGVQNPKFNNFFNEAMACETRLMSSVIRECKSVFEGLDSLVDVGGGTGSTAKVICEALPHLKCTVFDLPHVVANLPESENLNYVGGDMFHSIPSADAILLQWILHDWSNEDCVKILKRCREAIRSNDEGGKVIIIGIVIDVKNDEHELTETKLSFDMLMMVLFNGKERNEKEWEKLFLDADFSHYKITPIFGFRSLIEVYP
>g41869.t1
MSLKCAVELGISDVIFKHGQPITLPELVSALPIPSTKATCVHQLMRILVHSGIFATTKVHENQQEKEEEEEGYVLTPSSRLLLKDNTTNSLPFVAAILDPTLVTPCHFLGKWFMGNQNTAFETAYGMGFWDYGVQNPKFNNFFNEAMACETRLMSSVIRECKSVFEGLDSLVDVGGGTGSTAKVICEAFPHLKCTVFDLPHVVANLPESENLNYVGGDMFHSIPSADAILLQWILHDWSNEDCVKILKRCREAIRSNDEGGKVIIIGIVIDVKNDEHELTETKLSFDMLMMVLFNGKERNEKEWEKLFLDADFSHYKITPIFGFRSLIEVYP
>g5529.t1
MSVKCAVELGIPDIISKHGQPITLPELVSALPIPSTKATCVHQLMRILVHSGIFATTKVHENQQEEEEEEGYVLTPSSRLLLKDNTANLLPFVAAILDPTFVAPWHFLGKWFMGSQNTAFETAYGMGFWDYGVQNPKFNNLFNEAMASDSRLTSSVIRECKSVFEGLGSLVDVGGGTGSTAKVICEAFPHLKCTVFDLPHVVANLPESQNLNYVGGDMFHSIPSADAVLLKWILHDWSDEDCVKILKQCREAIRSNDEGGKVMIIDVVIDVKNDEHELTETKLSFDMLMMVLFNGKERNEKEWEKLFLAADFSHYKITPIFGFRSLIEVYP
>g47185.t1
MGTLSTWFQNTVDPTSFHTVHGKSFYDFLANEPSYGNMFNECMTNDTQLLTNLMIKECKGVFEGLKSLVDVGGGTGTVAKAIADAFPHSKCTVLDK
>g145521.t1
MSLKCAVQLGIPDIIHNHGKPITLSEVITALQIPPTKTTHMHRLMRILVHSGIFARKKVHENNQEEEEEEEEGYDLTPSSRLLIKDNITSLSPFAVAMLDPVLVTPWHFLGDWFQGNKKKNITAFETAHGMGFWDFMDQNSEFNKIFNEAMASDSGLMSSVIRDCKPVFEGLGSLVDVGGGTGLAAKIILEAFPHLKCTVFDFPHVVANLPESENLDFVGGNMFQSIPSADALLFKLVFHSISDEDCLKILKRCREAIPSKDKKGKVIIIDIVIDVKNDEHKLTETKLSFDMLMMALVTGRERSEKEWEKLFLEAGFSRYKITPMFGLRSLIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University