Enzyme
This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.
DFR
Enzyme
Dihydroflavonol 4-reductase
Enzyme name
EC:1.1.1.219
Entry
Oxidoreductases;Acting on the CH-OH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3S,4S)-leucoanthocyanidin:NADP+ 4-oxidoreductase
Sysname
a (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH + H+ [RN:R11500]
Reaction
(2R,3R)-dihydroflavonol [CPD:C21417];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Hamamelis virginiana
Species name
American witch-hazel (Hamamelis virginiana) is a species of eudicot in the family Hamamelidaceae (witch-hazel family).
Species info
Gene sequence info
Hamamelis_virginiana.DFR.fa
Download
>g8453.t1
MGSEAETVCVTGASGFIGSWLVMRLLEHGYRVRATVRDPENAKKVKHLLELPKAETHLTLWKADLSEEGSFDEAIQGCTGVFHVATPMDFESQDPENEVIKPTIAGMLDIMKACAKAKTVRRLVFTSSAGTVDVRERQQPVYDETCWSDMDFVRTVKMTGWMYFVSKTLAEQAAWEFAKENNIDFISIIPTLVVGPFLMPSMPPSLITALSPITGNEAHYGIIKQGQFVHLDDLCNAHIYLYENPKAHGRYICSSDEATILQVAKMLKQKYPEYNIPTSFKDVDENLKCVVFSSKKLTDLGFQFKYSLEDMFTEAVETCRAKGLLPLSHEKHVNDSS
>g8452.t1
MVRESETVCVTGASGFIGSWLVMRLLEHGYRVRATVRDPENMKKVKHLLELPKAKTHLTLWKADLSEEGSFDEAIQGCTGVFHVATPMDFESQDPENEVIKPTIAGMLGIMKACANAKTVRRLVFTSSAGTVNVEEHQKPVYDENCWSDMEFVRSKKMTGWMYFVSKTLAEKAAWEFAKENNIDFISIIPTLVVGPFLMPSMPPSLITALSPITGNEAHYGIIKQGQFVHLDDLCNSHIYLYENPKAQGRYICSSHDATILDIAKLLREKYPEYNVPTKFKDVDESLKCVAFSSKKLTDLGFQFKYSLEDMFTGAVETCRAKGLLPLSHEKLANGGLLPLSHEKLANGSS
>g74306.t1
MGSDRLRLFKADLQEEGSFEEAVKGCNGVFHVAASMEFNVDLAKENVESYVRSNIVDPAIRGTLNLLKSCLKSKSLKRVVFTSSISTLTAKDRDEKWRLVVDESCQRPIDHVWNTKTGGWVYVLSKLLTEEAAFQFVKENGIDLVSVITSTVGGPFLTPNIPSSIRVLLSPITGDTEFFPLLYAVNSRMGSIAVVHIEDICNAHIFLMEQAKAEGRYICCAYSCLISELTDHLAQEYPCSNMQRFVEDEHRSIPCEISSKKLRDLGFIYKHDLEDIIQHTVSCCVDFGHLPPIEK
>g25434.t1
MVKSGCKVCVTGGAGYVGSWLVKKLLEKGHTVHATLRNLDEASKVGLLKSFPYADTGLRLFEADIYKPDEFQTAIEGCDFVFHVATPLQHTNSSQYKNTTEAAIAGAKSIAMCCVRSKTVRRLIYTASVVAASPLKDDGTGGFKDSVDESCWTPLNLSFAYSNDPLK
>g88442.t1
MERNSYSKVCVTGGSGFIGSWLVKKLLEKGHTVHATLRNLEDTSKVGLLKSLPGADTRLILFQADIYNPNEFETAIIGCQFVFHVATPMLHNTHSSQFQDTSEAAVAGVRSIAGSCIRSQTVKRLIYTASVMSSSPLKEDGSGFKSCMDESCWTPLNLTIAYGYDFAMEYTKSKTLAEKEALGYNEIENGKLEVVSLTCALVGGDTLLPYVSGSVAVILSQITGSLSHYQSLRMLQELMGSIPLVHIDDVCEAHIFCMEKTSMKGRFLCALTDLTVGEIANYYQENYPEFKLPEE
>g100784.t1
MEGGAKTTVCVTGAAGFIGSWLVMRLLEKGYVVRATVRDPENMKKVKHLLELPKAKTHLTLWKADLAEEGSFDEAVKGCTGVFHVATPMDFESKDPENEVIKPTIEGVLGIIRSCAKAKTVRRLVFTSSAGTVNVQEHQLPEYDENCWSDLDFINHKKMTGWMYFKSKTLAEKAAWEAAKENNIDFISIIPTLVVGPFIMQSMPPSLITALSPLTGNEAHYSIVKQCQYVHLDDLCIAHIYLFEHPEAEGRFICSSHDATIYDIAKLIREKFPEYNVPTKFKGIDKNLKVVSFSSKKLTDLGFKFQYSLEDMYTGAIETCRQKGLIPHSN
>g34355.t1
MEGGAKTTVCVTGAAGFIGSWLVMRLLEKGYVVRATVRDPENMKKVKHLLELPKAKTHLTLWKADLAEEGSFDEAVKGCTGVFHVATPMDFESKDPENEVIKPTIEGVLGIIRSCAKAKTVRRLVFTSSAGTVNVQEHQLPEYDENCWSDLDFINHKKMTGWMYFKSKTLAEKAAWEAAKENNIDFISIIPTLVVGPFIMQSMPPSLITALSPLTGNEAHYSIVKQCQYVHLDDLCIAHIYLFEHPEAEGRFICSSHDATIYDIAKLIREKFPEYNVPTKFKGIDKNLKVVSFSSKKLTDLGFKFQYSLEDMYTGAIETCRQKGLIPHSN
MGSEAETVCVTGASGFIGSWLVMRLLEHGYRVRATVRDPENAKKVKHLLELPKAETHLTLWKADLSEEGSFDEAIQGCTGVFHVATPMDFESQDPENEVIKPTIAGMLDIMKACAKAKTVRRLVFTSSAGTVDVRERQQPVYDETCWSDMDFVRTVKMTGWMYFVSKTLAEQAAWEFAKENNIDFISIIPTLVVGPFLMPSMPPSLITALSPITGNEAHYGIIKQGQFVHLDDLCNAHIYLYENPKAHGRYICSSDEATILQVAKMLKQKYPEYNIPTSFKDVDENLKCVVFSSKKLTDLGFQFKYSLEDMFTEAVETCRAKGLLPLSHEKHVNDSS
>g8452.t1
MVRESETVCVTGASGFIGSWLVMRLLEHGYRVRATVRDPENMKKVKHLLELPKAKTHLTLWKADLSEEGSFDEAIQGCTGVFHVATPMDFESQDPENEVIKPTIAGMLGIMKACANAKTVRRLVFTSSAGTVNVEEHQKPVYDENCWSDMEFVRSKKMTGWMYFVSKTLAEKAAWEFAKENNIDFISIIPTLVVGPFLMPSMPPSLITALSPITGNEAHYGIIKQGQFVHLDDLCNSHIYLYENPKAQGRYICSSHDATILDIAKLLREKYPEYNVPTKFKDVDESLKCVAFSSKKLTDLGFQFKYSLEDMFTGAVETCRAKGLLPLSHEKLANGGLLPLSHEKLANGSS
>g74306.t1
MGSDRLRLFKADLQEEGSFEEAVKGCNGVFHVAASMEFNVDLAKENVESYVRSNIVDPAIRGTLNLLKSCLKSKSLKRVVFTSSISTLTAKDRDEKWRLVVDESCQRPIDHVWNTKTGGWVYVLSKLLTEEAAFQFVKENGIDLVSVITSTVGGPFLTPNIPSSIRVLLSPITGDTEFFPLLYAVNSRMGSIAVVHIEDICNAHIFLMEQAKAEGRYICCAYSCLISELTDHLAQEYPCSNMQRFVEDEHRSIPCEISSKKLRDLGFIYKHDLEDIIQHTVSCCVDFGHLPPIEK
>g25434.t1
MVKSGCKVCVTGGAGYVGSWLVKKLLEKGHTVHATLRNLDEASKVGLLKSFPYADTGLRLFEADIYKPDEFQTAIEGCDFVFHVATPLQHTNSSQYKNTTEAAIAGAKSIAMCCVRSKTVRRLIYTASVVAASPLKDDGTGGFKDSVDESCWTPLNLSFAYSNDPLK
>g88442.t1
MERNSYSKVCVTGGSGFIGSWLVKKLLEKGHTVHATLRNLEDTSKVGLLKSLPGADTRLILFQADIYNPNEFETAIIGCQFVFHVATPMLHNTHSSQFQDTSEAAVAGVRSIAGSCIRSQTVKRLIYTASVMSSSPLKEDGSGFKSCMDESCWTPLNLTIAYGYDFAMEYTKSKTLAEKEALGYNEIENGKLEVVSLTCALVGGDTLLPYVSGSVAVILSQITGSLSHYQSLRMLQELMGSIPLVHIDDVCEAHIFCMEKTSMKGRFLCALTDLTVGEIANYYQENYPEFKLPEE
>g100784.t1
MEGGAKTTVCVTGAAGFIGSWLVMRLLEKGYVVRATVRDPENMKKVKHLLELPKAKTHLTLWKADLAEEGSFDEAVKGCTGVFHVATPMDFESKDPENEVIKPTIEGVLGIIRSCAKAKTVRRLVFTSSAGTVNVQEHQLPEYDENCWSDLDFINHKKMTGWMYFKSKTLAEKAAWEAAKENNIDFISIIPTLVVGPFIMQSMPPSLITALSPLTGNEAHYSIVKQCQYVHLDDLCIAHIYLFEHPEAEGRFICSSHDATIYDIAKLIREKFPEYNVPTKFKGIDKNLKVVSFSSKKLTDLGFKFQYSLEDMYTGAIETCRQKGLIPHSN
>g34355.t1
MEGGAKTTVCVTGAAGFIGSWLVMRLLEKGYVVRATVRDPENMKKVKHLLELPKAKTHLTLWKADLAEEGSFDEAVKGCTGVFHVATPMDFESKDPENEVIKPTIEGVLGIIRSCAKAKTVRRLVFTSSAGTVNVQEHQLPEYDENCWSDLDFINHKKMTGWMYFKSKTLAEKAAWEAAKENNIDFISIIPTLVVGPFIMQSMPPSLITALSPLTGNEAHYSIVKQCQYVHLDDLCIAHIYLFEHPEAEGRFICSSHDATIYDIAKLIREKFPEYNVPTKFKGIDKNLKVVSFSSKKLTDLGFKFQYSLEDMYTGAIETCRQKGLIPHSN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University