Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Hamamelis virginiana
Species name
American witch-hazel (Hamamelis virginiana) is a species of eudicot in the family Hamamelidaceae (witch-hazel family).
Species info
Gene sequence info
Hamamelis_virginiana.4CL.fa
Download
>g42678.t1
MEKSGYGRDGIYRSLRPSLVLPKDPNLSMVSFLFRNSSSYPHKPALIDADTGESLSFAQFKSMTVKVSHGLIHQGIKKGDVVLIFAPNSIQFPLCFFGIVAMGAIATTANPLYTVSELSKQVKDSNPKLIVTVPELWEKVKGFNLPAIILGSKDNGFSNSRITFFSDLVKIGGPSSARDFPPGADSVKQSDIAALLYSSGTTGTSKGVILTHRNFIAASLMVTADQELAGEMHNVFLCFLPMFHVFGLSVITYGQLQRGNAIVSMGKFDFERVLSSVEKYRVTHLWVVPPVVIALAKQSAVKKYDLSSLKQIGSGAAPLGKDLMEECSKNFPQAMVVQGYGMTETCGVVSVESPKVRIRHTGSAGTLIPGVEAQVVSVDTLKPLPPNQLGEIWVRGPNMMQGYFNNPQATKQTIDKNGWVHTGDLGYFDEEGQLFVVDRIKELIKVKGFQVAPAELEGLLLSHPEILDAVVIPFPDAEAGEVPVAYVVRSPNSSLTEEDVMKFIADQVASFKRLRRVTFINSVPKSASGKILRRELIEKVRSKL
>g83022.t1
MEAKTEQLDEFIFRSKLPDIYIPSHLPLHLYCFENISQFSSRPCIINGATGEVYNYAEVELTARKVAAGLNKLGIQQGDVIMLLLQNSPEFVFAFLGASYRGAISTTANPFYTPAEIAKQASASKAKLIITQSSFAEKVREFAEENGVKIMCIDSSSSVVVDSSHFSELTEADENKIPAVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFHIYSLNSVLLCGLRVGAAILIMQKFEIITLMELVDKYKVTIAPFVPPIVLAIAKSPVVGNYDLSSIRTVMSGAAPMGKELEDTVRAMLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFGIKSGSCGTVVRNADMKIVDPDTGSSLPRNQAGEICIRGSQIMKGYLNDAEATERTIDREGWLHTGDIGYIDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMRDDSAGEVPVAFVIRSNGSKITEDEIKLYISKQVVFYKRISRVFFTDTIPKAPTGKILRKDLRAKLAAGLPN
>g23662.t1
MGTHLQTSPQYEEEEEEHIFRSRYSAVLVPENMTLPDFVLEDAELYADKVAFVEAATGKEFTYREVVRDTRRFAKALRSLGLRKGRVVVVVLPNVAEYAIVTLGIMAAGGVFSGANPAAHVSEIKKQVEVADAKLIVTNGTIYEKNEILDSILHEYKVKSIGVPVIVVGEDRVAGAICWDELLEAADRAGSDITNDTVDQNDLCALPFSSGTTGMQKGVMLTHRNLVANMCSTLFSVGPEIVGQVVTLGLMPFFHIYGITGICCATMRNKGKVVVMGRFELRMFMNALITQEVNFAPIVPPIILALVKNPIVEEFDLSRLKLKAIMTAAAPLAPELRAAFENKFPGVQVQEAYGLTEHSCITLTHGDPKKGHSNAKKNSVGFIIPNLEMKFIDPNTGRSLPNNTPGEICVRSQCVMQGYYKNEEETTRTIDENGWLHTGDVGYIDDDGDVFVVDRIKELIKYKGFQVAPAELEATLLSHSSVDDAAVVPLPDEEAGEIPAACVVMNPDAKESEEDIMNYVASNVAHYKQVRVVHFVDSIPKSPSGKIMRRLLRDKMVDKMQKTNPTAANAAAAAVAKILLE
>g22747.t1
MIISTNALLRQPKPVTCSHLITSPKPKRHRIIRCCMHNLNTTFKNSTTVIPIPPCNNISSDDDDNVHVFRSKLPDIPISNHLPLHTYCFHNLSEFSHRTCLILGSNGRTYSFAQTHLICQKTAAGFSKLGIKKGDVIMLLLQNCAEFVFSFMGASMIGAITTTANPFYTAAEILKQFTDSRPKLIITESQYIDKLRDIHGENPNMFTVITIDDPPTKDCLNFSVILEANESEIPQVSIDPDDPVALPYSSGTTGLPKGVILTHKSLITSVGQLVDGENPNLYLKEDDVVLCLLPLFHIFSLHTVLLCSLRAGAGVLLMQKFEIGVMLELIQRHRVSVAVLVPPMVRVLAKNPTVAGFDLSSIRVVLSGAEPLGKELEKALRLRFPHAILGQGYGMTEAGPVISMCLGFAKQSFPNKSGACGTVVRNAELKIIHLQTGCSLGHNQPGEICIRGHQIMKGYLNDAEATASTIDINGWLHTGDIGYVDDDGEVFIVDRVPPAELEALLMTHPSIVDAAVVPQKDDNIAGEVPVAFVVRCNGVELSEGAIKDFIAKQVVFYKKLHKVYFVHAIPKSPSGKILRKDLRAKLATA
>g22746.t1
MISVASVETQQPEVLSQNPTTIPYEKSSDDDAYIFRSKLPDIPISNHLPLHTYCFENLSEFSDRTCLILGSSGRTYSFAEIHLICQKTAAGLSNLGIKKGDVIMLLLQNCAEFVFSFIGASMIGAITTTANPFYTPAEIFKQFTASKTKLIITQSQYVDRLRDQGENYPKLGEDFSVITVDDPPENCLHFSVLSEANESEIPTVSIDPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKTDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSVAAVVPPLVLALAKNPMVASFDLSSIRVVLSGAAPLGKELEEALRSRVPQAVLGQGYGMTEAGPVLSMSLGFAKQPFPTKSGACGTVVRNAELKIIDPETSCSLNRNQPGEICIRGHQIMKGYLNDAEATATTIDPDGWLHTGDIGYVNDDEEIFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKVDAAGEVPVAFVVRSNGLELTEEAVKEFIAKQVVFYKKLHKVYFVHAIPKSPTGKILRKDLRAKLATASPLP
>g11665.t1
METVNIYYSPRPPISIPTDSSMVSFLFRKSSAFPNTLALVDADSHETLTFSDLKTTVAKLSHALLHLGIRKNDVVLIFAPNSIRFPVFFLAITALGAIATTANPLYTVAELSKQVKDSNPKLLVTVPELYDKVKGFNLPSVMLTSRSISSSSSDVFYYSDLIKKMDRISDLPEVSVAPTDVAALLYSSGTTGMSKGVVLTHRNFIATAVMVTADQDRYGDGNNVHLCFIPMFHIFGLSVITFAQLQRGNTVVSMGKFEIEKVLRAVEKYRVTHLFVVPPVMIALAKQSVVKKYDLSSLKELGSGAAPLGKEVMEECTKNFPHAGVIQGYGMTETCGIVSVENSREGSCLSGSTGTLVPGVEAQIIAVDTLKPLLPNQLGEIWVRGPNMMQGYYGNPQATKLTIDEHGWVHTGDIGYFDEKGHLFVVDRIKELIKYKGFQVAPAELEGLLLSHPEILDAAVIPFPDAEAGEVPIAYVVRSPNSSLTEEDIQKFIAKQVAPFKRLRRVTFINSVPKSASGKLLRRELIDKVRSKI
MEKSGYGRDGIYRSLRPSLVLPKDPNLSMVSFLFRNSSSYPHKPALIDADTGESLSFAQFKSMTVKVSHGLIHQGIKKGDVVLIFAPNSIQFPLCFFGIVAMGAIATTANPLYTVSELSKQVKDSNPKLIVTVPELWEKVKGFNLPAIILGSKDNGFSNSRITFFSDLVKIGGPSSARDFPPGADSVKQSDIAALLYSSGTTGTSKGVILTHRNFIAASLMVTADQELAGEMHNVFLCFLPMFHVFGLSVITYGQLQRGNAIVSMGKFDFERVLSSVEKYRVTHLWVVPPVVIALAKQSAVKKYDLSSLKQIGSGAAPLGKDLMEECSKNFPQAMVVQGYGMTETCGVVSVESPKVRIRHTGSAGTLIPGVEAQVVSVDTLKPLPPNQLGEIWVRGPNMMQGYFNNPQATKQTIDKNGWVHTGDLGYFDEEGQLFVVDRIKELIKVKGFQVAPAELEGLLLSHPEILDAVVIPFPDAEAGEVPVAYVVRSPNSSLTEEDVMKFIADQVASFKRLRRVTFINSVPKSASGKILRRELIEKVRSKL
>g83022.t1
MEAKTEQLDEFIFRSKLPDIYIPSHLPLHLYCFENISQFSSRPCIINGATGEVYNYAEVELTARKVAAGLNKLGIQQGDVIMLLLQNSPEFVFAFLGASYRGAISTTANPFYTPAEIAKQASASKAKLIITQSSFAEKVREFAEENGVKIMCIDSSSSVVVDSSHFSELTEADENKIPAVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFHIYSLNSVLLCGLRVGAAILIMQKFEIITLMELVDKYKVTIAPFVPPIVLAIAKSPVVGNYDLSSIRTVMSGAAPMGKELEDTVRAMLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFGIKSGSCGTVVRNADMKIVDPDTGSSLPRNQAGEICIRGSQIMKGYLNDAEATERTIDREGWLHTGDIGYIDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMRDDSAGEVPVAFVIRSNGSKITEDEIKLYISKQVVFYKRISRVFFTDTIPKAPTGKILRKDLRAKLAAGLPN
>g23662.t1
MGTHLQTSPQYEEEEEEHIFRSRYSAVLVPENMTLPDFVLEDAELYADKVAFVEAATGKEFTYREVVRDTRRFAKALRSLGLRKGRVVVVVLPNVAEYAIVTLGIMAAGGVFSGANPAAHVSEIKKQVEVADAKLIVTNGTIYEKNEILDSILHEYKVKSIGVPVIVVGEDRVAGAICWDELLEAADRAGSDITNDTVDQNDLCALPFSSGTTGMQKGVMLTHRNLVANMCSTLFSVGPEIVGQVVTLGLMPFFHIYGITGICCATMRNKGKVVVMGRFELRMFMNALITQEVNFAPIVPPIILALVKNPIVEEFDLSRLKLKAIMTAAAPLAPELRAAFENKFPGVQVQEAYGLTEHSCITLTHGDPKKGHSNAKKNSVGFIIPNLEMKFIDPNTGRSLPNNTPGEICVRSQCVMQGYYKNEEETTRTIDENGWLHTGDVGYIDDDGDVFVVDRIKELIKYKGFQVAPAELEATLLSHSSVDDAAVVPLPDEEAGEIPAACVVMNPDAKESEEDIMNYVASNVAHYKQVRVVHFVDSIPKSPSGKIMRRLLRDKMVDKMQKTNPTAANAAAAAVAKILLE
>g22747.t1
MIISTNALLRQPKPVTCSHLITSPKPKRHRIIRCCMHNLNTTFKNSTTVIPIPPCNNISSDDDDNVHVFRSKLPDIPISNHLPLHTYCFHNLSEFSHRTCLILGSNGRTYSFAQTHLICQKTAAGFSKLGIKKGDVIMLLLQNCAEFVFSFMGASMIGAITTTANPFYTAAEILKQFTDSRPKLIITESQYIDKLRDIHGENPNMFTVITIDDPPTKDCLNFSVILEANESEIPQVSIDPDDPVALPYSSGTTGLPKGVILTHKSLITSVGQLVDGENPNLYLKEDDVVLCLLPLFHIFSLHTVLLCSLRAGAGVLLMQKFEIGVMLELIQRHRVSVAVLVPPMVRVLAKNPTVAGFDLSSIRVVLSGAEPLGKELEKALRLRFPHAILGQGYGMTEAGPVISMCLGFAKQSFPNKSGACGTVVRNAELKIIHLQTGCSLGHNQPGEICIRGHQIMKGYLNDAEATASTIDINGWLHTGDIGYVDDDGEVFIVDRVPPAELEALLMTHPSIVDAAVVPQKDDNIAGEVPVAFVVRCNGVELSEGAIKDFIAKQVVFYKKLHKVYFVHAIPKSPSGKILRKDLRAKLATA
>g22746.t1
MISVASVETQQPEVLSQNPTTIPYEKSSDDDAYIFRSKLPDIPISNHLPLHTYCFENLSEFSDRTCLILGSSGRTYSFAEIHLICQKTAAGLSNLGIKKGDVIMLLLQNCAEFVFSFIGASMIGAITTTANPFYTPAEIFKQFTASKTKLIITQSQYVDRLRDQGENYPKLGEDFSVITVDDPPENCLHFSVLSEANESEIPTVSIDPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKTDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSVAAVVPPLVLALAKNPMVASFDLSSIRVVLSGAAPLGKELEEALRSRVPQAVLGQGYGMTEAGPVLSMSLGFAKQPFPTKSGACGTVVRNAELKIIDPETSCSLNRNQPGEICIRGHQIMKGYLNDAEATATTIDPDGWLHTGDIGYVNDDEEIFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKVDAAGEVPVAFVVRSNGLELTEEAVKEFIAKQVVFYKKLHKVYFVHAIPKSPTGKILRKDLRAKLATASPLP
>g11665.t1
METVNIYYSPRPPISIPTDSSMVSFLFRKSSAFPNTLALVDADSHETLTFSDLKTTVAKLSHALLHLGIRKNDVVLIFAPNSIRFPVFFLAITALGAIATTANPLYTVAELSKQVKDSNPKLLVTVPELYDKVKGFNLPSVMLTSRSISSSSSDVFYYSDLIKKMDRISDLPEVSVAPTDVAALLYSSGTTGMSKGVVLTHRNFIATAVMVTADQDRYGDGNNVHLCFIPMFHIFGLSVITFAQLQRGNTVVSMGKFEIEKVLRAVEKYRVTHLFVVPPVMIALAKQSVVKKYDLSSLKELGSGAAPLGKEVMEECTKNFPHAGVIQGYGMTETCGIVSVENSREGSCLSGSTGTLVPGVEAQIIAVDTLKPLLPNQLGEIWVRGPNMMQGYYGNPQATKLTIDEHGWVHTGDIGYFDEKGHLFVVDRIKELIKYKGFQVAPAELEGLLLSHPEILDAAVIPFPDAEAGEVPIAYVVRSPNSSLTEEDIQKFIAKQVAPFKRLRRVTFINSVPKSASGKLLRRELIDKVRSKI
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University