Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Gnetum montanum
Species name
Gnetum montanum is a species of seed plant in the family Gnetaceae.
Species info
Gene sequence info
Gnetum_montanum.FLS.fa
Download
>TnS001005307t01
MAMEKETLRVQPLAERLLAKGNCASLTLPAQYVQSDAERVPAVSGTVPVIDVSLLHGTAEERKETVNRIGAACREWGIFFAVNHGISGELIQELRTVGEAFFQLPQAEKEAYTARRVPDKPIAEGYAISAHGYGIGSNTDKTPKLGWVDYYFHLLAPPSQVNHAVWPTHHPRYR
>TnS000037493t03
MAPQVETVQSLAEKIELGRGSLPDEYVRAEAELAPAGPGAVPIIDVSLLHGDDAARRSAVKKIGAACREWGIFAAVNHGIPREVVRELQEVGEAFFALPAAEKQAYAAKKTQGRGLAQGYATAITRSHDGKLEWCDYYYHFLAPPSSVDHSFWPRHDPRYRKVTEAYSEKVKGMIDALMEALDEDLGVAKGSLANVMGGKENLHMEMKINYYPRCPDPNLALGLEPHSDMGILTVLVSNGPGLHVQAPDGSWFYADGEAVKADAEGEAIIVHVGDQLHIASNGAYHSVVHRTVVNDRSVRLSWPIFCVPPLDAVVGPLPQLLQGELNHSKTPSTVRYTPLTYVDYQRRKLTKKL
>TnS000037493t13
MAMEKETVRVQPLAERLTTKGNCGSLPLPAQYVQSEVERVPAVSGSVPVIDVSLLHGTAEERKETVNRIGAACREWGIFFAVNHGVSGKLLQEVRTMAEAFFQLPQAEKEAYKAHRTPGKTVAEGYATTADGYGSTPSTDTTRKLGWVDYYFHLLAPPSQVNHALWPGHHPRYRETMEKYNDNLKPVAKALMEAMDEHLGVNSGSLAKAMGGMDAMDVLLKLSYYPPCPRPDLVFGVTPHTDVAALTLLLTNGVPGLNVRRSDGVWVTVEGPGLADGSLIVHIGDQLKIASAGAFCNALHRSTVESDRVRMSWPVFYMPSPDTVVEPLPELLGQSSLSPEFTPLPYREFMHLKLSKKLTWI
>TnS000037493t14
MAMEKETLRVQPLAERLLAKGNCASLTLPAQYVQSEAEWVPAVSGSVPVIDVSLLHGTAEERKETVNRIGAACREWGIFFAVNHGISGELIQELRTVGEAFFQLPQAEKEAYTARRVPDKPIAEGYAISAHGYGIGSNTDKTPKLGWVDYYFHLLAPPSQVNHAVWPTHHPRYREITEKYNEGLTPVAKALMEAMDEHLGLNPGSLASLAKALGGMDEMDVLIKLSYYPPCPRPDLVFGVAPHTDLSALTLLLSNGLCGLNVCRSDGVWVAVEGAGVPDGALIVHIGDQLKIASAGAFCNVLHRSTVESERVRMSWPVFYMPPLDTVVEPVPELRGQSSLSPEFTPLPYREFMRLKFSNKNTGV
>TnS000863125t01
MAMEKETVRVQPLAERLTTKGNCGSLPLPAQYVQSEVERVPAVSGSVPVIDVSLLHGTAEERKETVNRIGAACREWGIFFAVNHGVSGKLLQEVRTMAEAFFQLPQAEKEAYKAHRTPGKPVPEGYATTADGYGSTPSTDTTRKLGWVDYYFHLLAPPSQVNHALWPGHHPRYREIMEKYKDNLQPVAKALMEAMDEHLGVNSGSLAKAMGGMDDMDVLLKLSYYPPCPRPDLVFGVPPHTDVAALTLLLTNGVPGLNVRRSDGVWVTVEGTGLADGSLIVHIGDQLKIASAGAFCNALHRSTVESDRVRMSWPVFYMPSPDTVVEPLPELLGQSSLSPEFTPLPYREFMHLKLGKKITWI
>TnS000536261t03
MEFPVVDLKPYLHWREQQQQHQEASAARLSEGEDEKEEEEAVVMKLCQQVALQLREAGALVVRDPRCRASDNDSFLDMMERYFEMPCDFKRQQERSHLHYQVGITPEGVERPRSTFDIDMQRKINEMPEKNRPKIPLGADPKWRYMWRVGPRPMKTRFKELNSEPVVPPGFPEWEETMNGWGSKMIATVEVVAEMAALGFGLPKESFTSRMQEGPHLLAPTGCDLSKHGNEGTLFAGYHYDLNFLTIHGRSRFPGLFIWLRNGKKVEVSVPVGCLLIQAGKQLEWLTGGDCQAGMHEVVVSKKTLAAVEEARQAGKSLWRVSSTVFGHIASDAILEPLGHFASCPSANNYPSICAGEFVEKELATINLKG
>TnS000636275t04
MESVKEISSSQPDHVPEKYIRDIQERPQLDESDDNAVEGEIPVLDMAAMNDEQTKQQELQRLAEACQEWGFFQIVNYGIPAELMDSILQVSRAFFELPLQEKQRYKRRPGKQFRGGYGQTFVVDENQKLDWCDLLSLSLAPRSNVDFSVWPCSSLAHFEGTVKSYSSHIRRVARFLLSALALTLGLHNNHFHNQMLSCGVVVVVLKSIILISTRIKMMMMIQ
>TnS000213865t01
MFVVDENKKLDWDDLLTVSLAPNSNVNMSVWPSSNVPHFEDTVKCYSHHICA
>TnS000213865t02
MAAMQDEQTKQQELQKVAKVCQEWGFFQIVNHGIPGELMDSISQVSKAFLELPLKEKEM
>TnS000514811t01
LMDSILQVSKAFFELPLEEKERYKPRPGNHFAGGYGQMFVVDENQKLDWGDLLTVSLAPDSNVDMSVWPSSNVPHFEDTVKCYSHHIRGLARFLLSALALNLGLHTNHFHSQMLSSGCGGSANADEHQNIDADDDIFMGMRINMYPQCPRPELVLGLSPHADGSCLTLLQDSNCVGLHVRHCGRWVAVQPVKRALVVNVGDLLEVMSNGRYMSVEHRAVPNKDCGRHSVAVFYSSAMDAAVEPAHELVMQDGAPRYRKFLHKEYVQHYMSGKLNGKHVLHDFAKIATS
>TnS000236247t01
MEGVKEISSSQPDHVPEKYIRDIQERPQLDETDDNAVEGEIPVLDMAAMNNEQTKQQELQRLAEACQEWGFFQIVNHGIPAELMDSILQVSRAFFKLPLQEKQRYKPRPGKHLGGGYGQRFVVDENQKLDWCDLLSLSLAPRSNVDFSVWPCSSLAHFEVFEAQTSAWANTTRRRQLPNAGAAGQQLCRITCAAPWEVESREASAGGPVVNIGDLVEVMNCGRHSVAVFYSSAMDSAVEPAHELVQQDGAPLYRRFIHKEYVQHYLSGKLNGKHVLHDFAKIATG
MAMEKETLRVQPLAERLLAKGNCASLTLPAQYVQSDAERVPAVSGTVPVIDVSLLHGTAEERKETVNRIGAACREWGIFFAVNHGISGELIQELRTVGEAFFQLPQAEKEAYTARRVPDKPIAEGYAISAHGYGIGSNTDKTPKLGWVDYYFHLLAPPSQVNHAVWPTHHPRYR
>TnS000037493t03
MAPQVETVQSLAEKIELGRGSLPDEYVRAEAELAPAGPGAVPIIDVSLLHGDDAARRSAVKKIGAACREWGIFAAVNHGIPREVVRELQEVGEAFFALPAAEKQAYAAKKTQGRGLAQGYATAITRSHDGKLEWCDYYYHFLAPPSSVDHSFWPRHDPRYRKVTEAYSEKVKGMIDALMEALDEDLGVAKGSLANVMGGKENLHMEMKINYYPRCPDPNLALGLEPHSDMGILTVLVSNGPGLHVQAPDGSWFYADGEAVKADAEGEAIIVHVGDQLHIASNGAYHSVVHRTVVNDRSVRLSWPIFCVPPLDAVVGPLPQLLQGELNHSKTPSTVRYTPLTYVDYQRRKLTKKL
>TnS000037493t13
MAMEKETVRVQPLAERLTTKGNCGSLPLPAQYVQSEVERVPAVSGSVPVIDVSLLHGTAEERKETVNRIGAACREWGIFFAVNHGVSGKLLQEVRTMAEAFFQLPQAEKEAYKAHRTPGKTVAEGYATTADGYGSTPSTDTTRKLGWVDYYFHLLAPPSQVNHALWPGHHPRYRETMEKYNDNLKPVAKALMEAMDEHLGVNSGSLAKAMGGMDAMDVLLKLSYYPPCPRPDLVFGVTPHTDVAALTLLLTNGVPGLNVRRSDGVWVTVEGPGLADGSLIVHIGDQLKIASAGAFCNALHRSTVESDRVRMSWPVFYMPSPDTVVEPLPELLGQSSLSPEFTPLPYREFMHLKLSKKLTWI
>TnS000037493t14
MAMEKETLRVQPLAERLLAKGNCASLTLPAQYVQSEAEWVPAVSGSVPVIDVSLLHGTAEERKETVNRIGAACREWGIFFAVNHGISGELIQELRTVGEAFFQLPQAEKEAYTARRVPDKPIAEGYAISAHGYGIGSNTDKTPKLGWVDYYFHLLAPPSQVNHAVWPTHHPRYREITEKYNEGLTPVAKALMEAMDEHLGLNPGSLASLAKALGGMDEMDVLIKLSYYPPCPRPDLVFGVAPHTDLSALTLLLSNGLCGLNVCRSDGVWVAVEGAGVPDGALIVHIGDQLKIASAGAFCNVLHRSTVESERVRMSWPVFYMPPLDTVVEPVPELRGQSSLSPEFTPLPYREFMRLKFSNKNTGV
>TnS000863125t01
MAMEKETVRVQPLAERLTTKGNCGSLPLPAQYVQSEVERVPAVSGSVPVIDVSLLHGTAEERKETVNRIGAACREWGIFFAVNHGVSGKLLQEVRTMAEAFFQLPQAEKEAYKAHRTPGKPVPEGYATTADGYGSTPSTDTTRKLGWVDYYFHLLAPPSQVNHALWPGHHPRYREIMEKYKDNLQPVAKALMEAMDEHLGVNSGSLAKAMGGMDDMDVLLKLSYYPPCPRPDLVFGVPPHTDVAALTLLLTNGVPGLNVRRSDGVWVTVEGTGLADGSLIVHIGDQLKIASAGAFCNALHRSTVESDRVRMSWPVFYMPSPDTVVEPLPELLGQSSLSPEFTPLPYREFMHLKLGKKITWI
>TnS000536261t03
MEFPVVDLKPYLHWREQQQQHQEASAARLSEGEDEKEEEEAVVMKLCQQVALQLREAGALVVRDPRCRASDNDSFLDMMERYFEMPCDFKRQQERSHLHYQVGITPEGVERPRSTFDIDMQRKINEMPEKNRPKIPLGADPKWRYMWRVGPRPMKTRFKELNSEPVVPPGFPEWEETMNGWGSKMIATVEVVAEMAALGFGLPKESFTSRMQEGPHLLAPTGCDLSKHGNEGTLFAGYHYDLNFLTIHGRSRFPGLFIWLRNGKKVEVSVPVGCLLIQAGKQLEWLTGGDCQAGMHEVVVSKKTLAAVEEARQAGKSLWRVSSTVFGHIASDAILEPLGHFASCPSANNYPSICAGEFVEKELATINLKG
>TnS000636275t04
MESVKEISSSQPDHVPEKYIRDIQERPQLDESDDNAVEGEIPVLDMAAMNDEQTKQQELQRLAEACQEWGFFQIVNYGIPAELMDSILQVSRAFFELPLQEKQRYKRRPGKQFRGGYGQTFVVDENQKLDWCDLLSLSLAPRSNVDFSVWPCSSLAHFEGTVKSYSSHIRRVARFLLSALALTLGLHNNHFHNQMLSCGVVVVVLKSIILISTRIKMMMMIQ
>TnS000213865t01
MFVVDENKKLDWDDLLTVSLAPNSNVNMSVWPSSNVPHFEDTVKCYSHHICA
>TnS000213865t02
MAAMQDEQTKQQELQKVAKVCQEWGFFQIVNHGIPGELMDSISQVSKAFLELPLKEKEM
>TnS000514811t01
LMDSILQVSKAFFELPLEEKERYKPRPGNHFAGGYGQMFVVDENQKLDWGDLLTVSLAPDSNVDMSVWPSSNVPHFEDTVKCYSHHIRGLARFLLSALALNLGLHTNHFHSQMLSSGCGGSANADEHQNIDADDDIFMGMRINMYPQCPRPELVLGLSPHADGSCLTLLQDSNCVGLHVRHCGRWVAVQPVKRALVVNVGDLLEVMSNGRYMSVEHRAVPNKDCGRHSVAVFYSSAMDAAVEPAHELVMQDGAPRYRKFLHKEYVQHYMSGKLNGKHVLHDFAKIATS
>TnS000236247t01
MEGVKEISSSQPDHVPEKYIRDIQERPQLDETDDNAVEGEIPVLDMAAMNNEQTKQQELQRLAEACQEWGFFQIVNHGIPAELMDSILQVSRAFFKLPLQEKQRYKPRPGKHLGGGYGQRFVVDENQKLDWCDLLSLSLAPRSNVDFSVWPCSSLAHFEVFEAQTSAWANTTRRRQLPNAGAAGQQLCRITCAAPWEVESREASAGGPVVNIGDLVEVMNCGRHSVAVFYSSAMDSAVEPAHELVQQDGAPLYRRFIHKEYVQHYLSGKLNGKHVLHDFAKIATG
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University