Enzyme
A cytochrome P-450 (heme-thiolate) protein found in plants. The 3',5'-dihydroxyflavanone is formed via the 3'-hydroxyflavanone. In Petunia hybrida the enzyme acts on naringenin, eriodictyol, dihydroquercetin (taxifolin) and dihydrokaempferol (aromadendrin). The enzyme catalyses the hydroxylation of 5,7,4'-trihydroxyflavanone (naringenin) at either the 3' position to form eriodictyol or at both the 3' and 5' positions to form 5,7,3',4',5'-pentahydroxyflavanone (dihydrotricetin). The enzyme also catalyses the hydroxylation of 3,5,7,3',4'-pentahydroxyflavanone (taxifolin) at the 5' position, forming ampelopsin.
F3'5'H
Enzyme
Flavanoid 3',5'-hydroxylase
Enzyme name
EC:1.14.14.81
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Class
flavanone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3',5'-dihydroxylating)
Sysname
a flavanone + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = a 3',5'-dihydroxyflavanone + 2 [oxidized NADPH---hemoprotein reductase] + 2 H2O (overall reaction) [RN:R10069];(1a) a flavanone + [reduced NADPH---hemoprotein reductase] + O2 = a 3'-hydroxyflavanone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07369];(1b) a 3'-hydroxyflavanone + [reduced NADPH---hemoprotein reductase] + O2 = a 3',5'-dihydroxyflavanone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07370]
Reaction
3',5'-dihydroxyflavanone [CPD:C15609];[oxidized NADPH---hemoprotein reductase] [CPD:C03161];H2O [CPD:C00001];3'-hydroxyflavanone [CPD:C15204]
Product
Species information
Gnetum montanum
Species name
Gnetum montanum is a species of seed plant in the family Gnetaceae.
Species info
Gene sequence info
Gnetum_montanum.F3_5_H.fa
Download
>TnS000979079t03
MECAEALTWVAALAVAYVVWWKLLGSLPRQRSKQLPPGPRGLPIVGCLPHLGAAPHAALTQLARRYGPVMYLKLGTSGIVVATSPSAAKAFGKTLDANFCGRPQDSATANILGYSCRSMVWASYGPRWAHLRKLCNLHVLGPKVEQLLAVRAFEVRALVRNIFGHARDDPLCLPEFLNICTANILGQLVLGRRVFEQEGGKASEFKTMVVELMILSGAFNLADFIPQIAWMDVQGIQRKMKSVHKRWDALISEFIAEHKGSQRTNQNKQQQQQDFVDILIQEGVDERDIKALVLDLFAAGTDTSSSVTEWVMAELIDNPKIMAKLQSEIDGVVGRDKVLQQADVARLPYLQAVCKEGLRKHPPTPLGLPRIAAEACEVGGYFIPKNTRLLINIWGMLKDPAVWPNPEIFDPERFVGSNLSPFGNDFELIPFGTGRRICAGARMGLNSVEFMVGSLVQAFNWDVANPADRPINMDETFGLTLQKTVQLVAVATPRLSANMYQI
>TnS000500993t01
TFDELYAMLETLLNEPLAEPPEKAEQRNYLVAVLLASRRAEFGLDDVLGFVVTMFGAGTDTVTISVEWIKTEMMQNVEFLAKCHVKMHCTVGRDRRVVEMDGERLSYLNAVIKKTYRLRPTLLLLIPHLAVEEAEVGGYVVPQGSRLIVNMWAIGRDSANWDSPETFNLERFEWSLPAGMSPADVDVDGHFGMVYTTIVPLRIQPSHSLPTHLYYDGSLPKTI
>TnS000904187t04
MTVEWIMTELMQNLEFLAKCHVEMHCTVGRDRRVAETDAERLPYLNAVDGHFGMVYKKIVQPSHRLPTHLY
>TnS000207311t01
NDFELIPFGKIYAHAQMGLNNIEFMVGSLVQTFHWDVANLVDCPMWTFDWDVVNLMDRPIHMDEAFSQAIFF
>TnS000189017t01
MGMLKDPALWPNLEIFDLKQFVGSNLLSFGNNFELIPFRKICARAQMGLNNVEFKVGSLVQTFHWDVANLVDRPMWTFDWDD
>TnS000844793t02
MWPNPKIFNPKHFVGSNLSSFSNYFNLILFSTSRRICIGTRMGFNNVEFTMRSLVQAFDWDVANPTNHPMNMDEAFGLTLHK
>TnS000686249t04
LLLRRRMFDQEEGKASEFKTMVVELMTLSGAFNLADFIPQIVCMDVQGIQRKMKLCIIVCNVYYQLSCKNLRKKVGYLS
>TnS000217277t01
CFLVIAVASGASPIRARGGGKASEFKKMAVELMTLSGPFNLADFIPQIVWMGMQGIQLKMKLCIIV
>TnS000632285t01
MVVELMTLSGAFNLADFIPQIAWMDVQGIQRRNHSLFIIFLLITCEHDGCITQKLKYQSDTTCKPCDQTNEVFEPYMPNTDDDKRCLSICPVHFPLLLRNGASDGFTSLAYQTNYGPFFTNGACRFLS
>TnS000875779t01
IFYLKQFVGSNLLSFGNDFELILFGKIYARAQMGLNNIEFMVGSLVKTFHWDVANLVDHPMWTFDWDVVHLLDHPIHMDEAFCQAIFF
>TnS000501459t01
MWPNPKIFNPERFVGSNPSSFSNDFKLILFSTSCRIYIGTQMGLNNVEFTVRSLVQAFDWDVANPVDRPMNMDEAFGLTLQK
MECAEALTWVAALAVAYVVWWKLLGSLPRQRSKQLPPGPRGLPIVGCLPHLGAAPHAALTQLARRYGPVMYLKLGTSGIVVATSPSAAKAFGKTLDANFCGRPQDSATANILGYSCRSMVWASYGPRWAHLRKLCNLHVLGPKVEQLLAVRAFEVRALVRNIFGHARDDPLCLPEFLNICTANILGQLVLGRRVFEQEGGKASEFKTMVVELMILSGAFNLADFIPQIAWMDVQGIQRKMKSVHKRWDALISEFIAEHKGSQRTNQNKQQQQQDFVDILIQEGVDERDIKALVLDLFAAGTDTSSSVTEWVMAELIDNPKIMAKLQSEIDGVVGRDKVLQQADVARLPYLQAVCKEGLRKHPPTPLGLPRIAAEACEVGGYFIPKNTRLLINIWGMLKDPAVWPNPEIFDPERFVGSNLSPFGNDFELIPFGTGRRICAGARMGLNSVEFMVGSLVQAFNWDVANPADRPINMDETFGLTLQKTVQLVAVATPRLSANMYQI
>TnS000500993t01
TFDELYAMLETLLNEPLAEPPEKAEQRNYLVAVLLASRRAEFGLDDVLGFVVTMFGAGTDTVTISVEWIKTEMMQNVEFLAKCHVKMHCTVGRDRRVVEMDGERLSYLNAVIKKTYRLRPTLLLLIPHLAVEEAEVGGYVVPQGSRLIVNMWAIGRDSANWDSPETFNLERFEWSLPAGMSPADVDVDGHFGMVYTTIVPLRIQPSHSLPTHLYYDGSLPKTI
>TnS000904187t04
MTVEWIMTELMQNLEFLAKCHVEMHCTVGRDRRVAETDAERLPYLNAVDGHFGMVYKKIVQPSHRLPTHLY
>TnS000207311t01
NDFELIPFGKIYAHAQMGLNNIEFMVGSLVQTFHWDVANLVDCPMWTFDWDVVNLMDRPIHMDEAFSQAIFF
>TnS000189017t01
MGMLKDPALWPNLEIFDLKQFVGSNLLSFGNNFELIPFRKICARAQMGLNNVEFKVGSLVQTFHWDVANLVDRPMWTFDWDD
>TnS000844793t02
MWPNPKIFNPKHFVGSNLSSFSNYFNLILFSTSRRICIGTRMGFNNVEFTMRSLVQAFDWDVANPTNHPMNMDEAFGLTLHK
>TnS000686249t04
LLLRRRMFDQEEGKASEFKTMVVELMTLSGAFNLADFIPQIVCMDVQGIQRKMKLCIIVCNVYYQLSCKNLRKKVGYLS
>TnS000217277t01
CFLVIAVASGASPIRARGGGKASEFKKMAVELMTLSGPFNLADFIPQIVWMGMQGIQLKMKLCIIV
>TnS000632285t01
MVVELMTLSGAFNLADFIPQIAWMDVQGIQRRNHSLFIIFLLITCEHDGCITQKLKYQSDTTCKPCDQTNEVFEPYMPNTDDDKRCLSICPVHFPLLLRNGASDGFTSLAYQTNYGPFFTNGACRFLS
>TnS000875779t01
IFYLKQFVGSNLLSFGNDFELILFGKIYARAQMGLNNIEFMVGSLVKTFHWDVANLVDHPMWTFDWDVVHLLDHPIHMDEAFCQAIFF
>TnS000501459t01
MWPNPKIFNPERFVGSNPSSFSNDFKLILFSTSCRIYIGTQMGLNNVEFTVRSLVQAFDWDVANPVDRPMNMDEAFGLTLQK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University