Enzyme
Isolated from the plants Perilla frutescens and Gentiana triflora (clustered gentian). Acts on a range of anthocyanidin 3-O-glucosides, 3,5-di-O-glucosides and cyanidin 3-rutinoside. It did not act on delphinidin 3,3',7-tri-O-glucoside. Recombinant Perilla frutescens enzyme could utilize caffeoyl-CoA but not malonyl-CoA as alternative acyl donor.
ANAT
Enzyme
Anthocyanin acyltransferase
Enzyme name
EC:2.3.1.215
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanin-3-O-glucoside 6''-O-acyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3-O-beta-D-glucoside = CoA + an anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [RN:R10237]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [CPD:C20461]
Product
Species information
Gelsemium sempervirens
Species name
Gelsemium sempervirens is a species of eudicot in the family Gelsemiaceae.
Species info
Gene sequence info
Gelsemium_sempervirens.ANAT.fa
Download
>CRO_T108280
MYSEIINGSDSDGGGFINVAISIGEAIYKSMNNVEAEMKWNGKWEEGLPFSVSGSTAFDIYMVRDFGWRKMEKFESLAIDIKRSMYIGRLGKFAEFYKPPRKTRNKKPNFNKITCYKCGKLGHKANACRSKKINEILDQFPEIDKELKEKLSISLVQIDSSXDYXPDDFQDFSSTDEGNISSPCACKVINMIKHSDNTVKDQKELIFDIIQDLPPEAQKKQLEKLESPHSSGRIQFCPLNRAFQHFQNV
>CRO_T110809
MALNVIEQCRVAPPSGTAAEHSLPLTFWDARWLNFHPVPVILFYKFSQSKYNFIETTIPKLKESLSITLKYYLPLASNLIFPDSGKPELKFVEGNSVPIIFAVSNDDFNYLVGDPPKNCNRFYPLVPQVPPAFTESGSTKTPVFALQVTLFPEIGISIAYSMLHLLGDASSLVGFLKAWGSIFKFGGNENFIPTSSLPFYDRSMIKDPYNLLEKILIEKKKQKNRTWDLSEIREIAADKVRATFVMHQTDIKHLKNYVLSKRPKVVHLSSWTVTCAYLWVCWLKSMEAIGEQVDDSETEYFLSAVNYRARLDPPMPSTYFGSAIVPSITKARHGEIIGPDGFATAAALIGEAIHRRVSDRESVLKGDWLNEVEEVKKGRFLGTNGSPLFDTYGTDFGWGKAEKYESISIDIDGGMAIERSRKFEGGLEIGLSLPGKRMDAFGAFFNSFIKTLLLQPKI
>CRO_T132130
MGGLIYKLNGDNVRLLDESSLLSFYDRSTIKDPYNLLKKILNYNKEQKNSSTTNDLFKNSNKVRATYVMHQTDIKNLKNYVLSKRPNAIHISSWIVTCAYLWICWLKSLEAIGEKMDVTKNQYFMCPVNYRVRLHPPLRMTYFGNCIAPSLAKLMYGEIVESDGLINAAVSIGEAIHTSINDMETVWNGTEYAKGEEVTQGRLFIVNGSPLLFDTYDVDFGWGKMNKFEPMVTDDGSLFIHGSRKFQSAVEIGLSLEQKIMDAFATIFNSHISYYFGKSNLSFNHA
>CRO_T132132
MVALKVLERCRVAPPPGTVAATDHTTLPLTFWDARWLNFHPCPGILFYKFPCTKTHFIDTVIPKLKQSLSLTLKHYFPLATNLVFNEESGKPLLEFMEGNSVSVLLAESSDDFNYLVGDDHSRNCDRFYSLIPKIPPPFIEFGIEKTPLSALQTWSLIYKLNGDNVGFLDKSSLLPFYDRNTIKDPYNLLEKILNDKKKQKNSTTTNDLSENSNKVRATFVMHQTDIKNLKNYIFSKRPNAIHISSWTVTCAYLWICWLKSLEAIGEKVDEKENQYFLCPVNYRARLHPPLPMTYLGNCIGPSVAKLVHDEIVESDGLINAAVSIGEAIHTSINDLENVWNGTVYAKVDEMKQGRVFIVSGSPLLFDTHGVDFGWGKMNTFEPMITDIDGSLFIQGSRKFQGAVEISLSLEKKRMDAFATIFNTHLSYDFGKSNLSFNHA
>CRO_T106365
MAPNLRVKVLEQCKVSPSPPDSMAELTIPLSFFDMSWLSFQMLQMVLFYDFPHIMLETNIIPDLKSSLSLFLKHYLPFAGNLIYPTNSPNKPILRYIQGDSIHVTFAESNQDFHYLKGNQPRNADDFFPLLPQSPQISKESDAKVIPLAAIQVTFFPGSGLSIGLIGHHILGDGIAILNGVKAWSSMHKLGTEKANKLLAGKCLAILDRSLISDKDGLAEKHWNKMKNNIRLINFDQPNNPLISNGNNKVLATFVLEKIDLEKLKNFVVQKSRKKDIYVSSFTVTCAYFWTCFVKSGISIGEEIVDNNTDEYFIAAIDCRSRVKPRVPENYFGNCIAPCIIKSNHELLASNEEGFVNAAIIIGDALSKKVQNEKGLLEDAKSWLSNIESVKMGRFFGVTGSPKMEIYELDYGWGKPEKVEILSILSNQTMSLNKARRSEGGLEVGLALPKTKMEAFEKIFNNGLMNMKF
>CRO_T120886
MAEIPNLTILEHCLVAPPQVTASDAELSIPLTFFDTQFWNSNPMHELLFYEFRHSKTHFVENIIPSLKESLSFALKHFLPMAGNLIYDSESGSRELRYEIRDSVPLTFAESDDDFKYLVKNHPRIADRFYPLVPQLPPLSVHSSGKTKTPLFALQVTIFSNAGICVAWSYRHVLGDASTTIGFMKAWASIFSKSTRNSEVLGHNFVPVYDRSTIQDPFHLEVVLFNAMKMFKPQEPPSVTTILSNNYKVRATFNMSQIDIQKLKNLVLTKEPNPPHVSSLTVTCAYAWTCWLRSIDAVVKEIEGDTINNEPQFLSVPVDFRQRLDPPLPQTYFGNCVLPCIVPFSCVEVVGNGNEGFFKVAIQIGEAVKKMLNNKEGVVKGNWLSELRKIASGNILAVVGSPKYDGYDVDFGWGKANKFESLAVDESKAMSISKSRKFEGALELSLCLPSERMDAFVAFFIDGLKKL
>CRO_T124169
MSSLRAKERSSNNERQLARTAKSVEDFQHLVGNYSRNASRFYSLIPQLPPESVESGGARVSAIFAIQVALFPNYGITIGFANQHVIGDANTVTRFIRAWASINKLEGETELFDNKFLPFYDRERIEDPYKLAIVLFNEMKKPQPWQESESDFRKVPMKNSNLRATSVMSQDKIQMLKKYAIENMPKLVHPSSFVLTCAYIRTSWLKSLEEIGEENVDEHEAEYFGFMADCRARLSPSLPDNYFGNCIVPCIAKSTRGELCGPQGILIAAEEIGMSIGKRFNDKDGILKGVWFYKSSEINWKRLFIVSGSTKHDTNDADFGWGKPEKYECISIDSDGAMSINKSGKYEGGLEIGLSLPTNQMDIFADIFASGLKNL
>CRO_T125681
MAQVINVLEHCRLPPSTSSPAAIAEFTLPLTFYDVIWINFHPIQRLLFYKLPYPKSHFTGTIIPKLKQSLSIALKHFLPIAGNLIIPLDSGFPQLRYAKGDTLSVTFAESFEDFQDLVGNHPRNCSRFYSLIPQLPPESVESGGARVSAVFAVQVTLFPNYGITIGFANRHVIGDANTVTRFIRAWASINKLEGETELFDNNDNKSFLPFYGREVIEDPYKLGTLVFNEMKKPQPWQLESDNSEVPTKNFNLRATFVMSQDEIQMLKKSAKENMPDLVHPSSFVLTCAYIWTSWLKSQEALGEEIVNENETEYFLFAADFRARLSPLLPDNYFGNCILSCIAKSTRGELHGPKGLWIAAEKIGMSIGKRLNDNEGILKGVWFNELCEINWKRLFSVSGSTKHDTNDADFGWGKPEKYECISIDSDGAMSINKSGKYEGGLEIGLSLPKKQMDIFADILASGLKNL
>CRO_T125680
MAQVIDVLEHCRLPPSTSSSAATAEFTLPLTFYDVVWINFYPIQRLLFYKLPYPKSHFTGTIIPKLKQSLSIALKHFLPLAGNLIIPLDSGFPQLRYAKGDTLSVTFAESFEDFQDLVGNHPRNCSRFYSLIPQLRPESIESGGARVSAVLAVQVTLFPNYGITIGSVHQHVIGDANVVTGFMRTWASINKFDGETELFDNNDNKSFLPFYGREVLEDPYKLTTLIFNEMAKPNPRQESESNVSKVPTKNFNFNLRVTFVMSQDEIEMLKKSAKENMPDLVHPSSFVLTCAYIWTSWLKSMEAIGEEIKVDENETEYFLFAADYRARLSPPLPANYFGNCIVPCIAKSTRGELCGPRGLSIAAENIGIAIRERLNDQEKILKGVWFYELREINWKRLLSVSGSTKHDTYDADFGWGKPEKYECVSIDASQAVSINKSGKYEVGFEIGLSLPKNQMDTFADIFASGLKNL
MYSEIINGSDSDGGGFINVAISIGEAIYKSMNNVEAEMKWNGKWEEGLPFSVSGSTAFDIYMVRDFGWRKMEKFESLAIDIKRSMYIGRLGKFAEFYKPPRKTRNKKPNFNKITCYKCGKLGHKANACRSKKINEILDQFPEIDKELKEKLSISLVQIDSSXDYXPDDFQDFSSTDEGNISSPCACKVINMIKHSDNTVKDQKELIFDIIQDLPPEAQKKQLEKLESPHSSGRIQFCPLNRAFQHFQNV
>CRO_T110809
MALNVIEQCRVAPPSGTAAEHSLPLTFWDARWLNFHPVPVILFYKFSQSKYNFIETTIPKLKESLSITLKYYLPLASNLIFPDSGKPELKFVEGNSVPIIFAVSNDDFNYLVGDPPKNCNRFYPLVPQVPPAFTESGSTKTPVFALQVTLFPEIGISIAYSMLHLLGDASSLVGFLKAWGSIFKFGGNENFIPTSSLPFYDRSMIKDPYNLLEKILIEKKKQKNRTWDLSEIREIAADKVRATFVMHQTDIKHLKNYVLSKRPKVVHLSSWTVTCAYLWVCWLKSMEAIGEQVDDSETEYFLSAVNYRARLDPPMPSTYFGSAIVPSITKARHGEIIGPDGFATAAALIGEAIHRRVSDRESVLKGDWLNEVEEVKKGRFLGTNGSPLFDTYGTDFGWGKAEKYESISIDIDGGMAIERSRKFEGGLEIGLSLPGKRMDAFGAFFNSFIKTLLLQPKI
>CRO_T132130
MGGLIYKLNGDNVRLLDESSLLSFYDRSTIKDPYNLLKKILNYNKEQKNSSTTNDLFKNSNKVRATYVMHQTDIKNLKNYVLSKRPNAIHISSWIVTCAYLWICWLKSLEAIGEKMDVTKNQYFMCPVNYRVRLHPPLRMTYFGNCIAPSLAKLMYGEIVESDGLINAAVSIGEAIHTSINDMETVWNGTEYAKGEEVTQGRLFIVNGSPLLFDTYDVDFGWGKMNKFEPMVTDDGSLFIHGSRKFQSAVEIGLSLEQKIMDAFATIFNSHISYYFGKSNLSFNHA
>CRO_T132132
MVALKVLERCRVAPPPGTVAATDHTTLPLTFWDARWLNFHPCPGILFYKFPCTKTHFIDTVIPKLKQSLSLTLKHYFPLATNLVFNEESGKPLLEFMEGNSVSVLLAESSDDFNYLVGDDHSRNCDRFYSLIPKIPPPFIEFGIEKTPLSALQTWSLIYKLNGDNVGFLDKSSLLPFYDRNTIKDPYNLLEKILNDKKKQKNSTTTNDLSENSNKVRATFVMHQTDIKNLKNYIFSKRPNAIHISSWTVTCAYLWICWLKSLEAIGEKVDEKENQYFLCPVNYRARLHPPLPMTYLGNCIGPSVAKLVHDEIVESDGLINAAVSIGEAIHTSINDLENVWNGTVYAKVDEMKQGRVFIVSGSPLLFDTHGVDFGWGKMNTFEPMITDIDGSLFIQGSRKFQGAVEISLSLEKKRMDAFATIFNTHLSYDFGKSNLSFNHA
>CRO_T106365
MAPNLRVKVLEQCKVSPSPPDSMAELTIPLSFFDMSWLSFQMLQMVLFYDFPHIMLETNIIPDLKSSLSLFLKHYLPFAGNLIYPTNSPNKPILRYIQGDSIHVTFAESNQDFHYLKGNQPRNADDFFPLLPQSPQISKESDAKVIPLAAIQVTFFPGSGLSIGLIGHHILGDGIAILNGVKAWSSMHKLGTEKANKLLAGKCLAILDRSLISDKDGLAEKHWNKMKNNIRLINFDQPNNPLISNGNNKVLATFVLEKIDLEKLKNFVVQKSRKKDIYVSSFTVTCAYFWTCFVKSGISIGEEIVDNNTDEYFIAAIDCRSRVKPRVPENYFGNCIAPCIIKSNHELLASNEEGFVNAAIIIGDALSKKVQNEKGLLEDAKSWLSNIESVKMGRFFGVTGSPKMEIYELDYGWGKPEKVEILSILSNQTMSLNKARRSEGGLEVGLALPKTKMEAFEKIFNNGLMNMKF
>CRO_T120886
MAEIPNLTILEHCLVAPPQVTASDAELSIPLTFFDTQFWNSNPMHELLFYEFRHSKTHFVENIIPSLKESLSFALKHFLPMAGNLIYDSESGSRELRYEIRDSVPLTFAESDDDFKYLVKNHPRIADRFYPLVPQLPPLSVHSSGKTKTPLFALQVTIFSNAGICVAWSYRHVLGDASTTIGFMKAWASIFSKSTRNSEVLGHNFVPVYDRSTIQDPFHLEVVLFNAMKMFKPQEPPSVTTILSNNYKVRATFNMSQIDIQKLKNLVLTKEPNPPHVSSLTVTCAYAWTCWLRSIDAVVKEIEGDTINNEPQFLSVPVDFRQRLDPPLPQTYFGNCVLPCIVPFSCVEVVGNGNEGFFKVAIQIGEAVKKMLNNKEGVVKGNWLSELRKIASGNILAVVGSPKYDGYDVDFGWGKANKFESLAVDESKAMSISKSRKFEGALELSLCLPSERMDAFVAFFIDGLKKL
>CRO_T124169
MSSLRAKERSSNNERQLARTAKSVEDFQHLVGNYSRNASRFYSLIPQLPPESVESGGARVSAIFAIQVALFPNYGITIGFANQHVIGDANTVTRFIRAWASINKLEGETELFDNKFLPFYDRERIEDPYKLAIVLFNEMKKPQPWQESESDFRKVPMKNSNLRATSVMSQDKIQMLKKYAIENMPKLVHPSSFVLTCAYIRTSWLKSLEEIGEENVDEHEAEYFGFMADCRARLSPSLPDNYFGNCIVPCIAKSTRGELCGPQGILIAAEEIGMSIGKRFNDKDGILKGVWFYKSSEINWKRLFIVSGSTKHDTNDADFGWGKPEKYECISIDSDGAMSINKSGKYEGGLEIGLSLPTNQMDIFADIFASGLKNL
>CRO_T125681
MAQVINVLEHCRLPPSTSSPAAIAEFTLPLTFYDVIWINFHPIQRLLFYKLPYPKSHFTGTIIPKLKQSLSIALKHFLPIAGNLIIPLDSGFPQLRYAKGDTLSVTFAESFEDFQDLVGNHPRNCSRFYSLIPQLPPESVESGGARVSAVFAVQVTLFPNYGITIGFANRHVIGDANTVTRFIRAWASINKLEGETELFDNNDNKSFLPFYGREVIEDPYKLGTLVFNEMKKPQPWQLESDNSEVPTKNFNLRATFVMSQDEIQMLKKSAKENMPDLVHPSSFVLTCAYIWTSWLKSQEALGEEIVNENETEYFLFAADFRARLSPLLPDNYFGNCILSCIAKSTRGELHGPKGLWIAAEKIGMSIGKRLNDNEGILKGVWFNELCEINWKRLFSVSGSTKHDTNDADFGWGKPEKYECISIDSDGAMSINKSGKYEGGLEIGLSLPKKQMDIFADILASGLKNL
>CRO_T125680
MAQVIDVLEHCRLPPSTSSSAATAEFTLPLTFYDVVWINFYPIQRLLFYKLPYPKSHFTGTIIPKLKQSLSIALKHFLPLAGNLIIPLDSGFPQLRYAKGDTLSVTFAESFEDFQDLVGNHPRNCSRFYSLIPQLRPESIESGGARVSAVLAVQVTLFPNYGITIGSVHQHVIGDANVVTGFMRTWASINKFDGETELFDNNDNKSFLPFYGREVLEDPYKLTTLIFNEMAKPNPRQESESNVSKVPTKNFNFNLRVTFVMSQDEIEMLKKSAKENMPDLVHPSSFVLTCAYIWTSWLKSMEAIGEEIKVDENETEYFLFAADYRARLSPPLPANYFGNCIVPCIAKSTRGELCGPRGLSIAAENIGIAIRERLNDQEKILKGVWFYELREINWKRLLSVSGSTKHDTYDADFGWGKPEKYECVSIDASQAVSINKSGKYEVGFEIGLSLPKNQMDTFADIFASGLKNL
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University