Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Gelsemium sempervirens
Species name
Gelsemium sempervirens is a species of eudicot in the family Gelsemiaceae.
Species info
Gene sequence info
Gelsemium_sempervirens.HI4_OMT.fa
Download
>CRO_T104106
MEVQSAEFRKAQVHIWSQSFSFITCASLKCALQLGIADAIENHGKPMALSDLTNTLQINPSKSQYLPRLMRLLVNSGFFSEQQLGDAGGGENVYGLTPSMRLLLKNEPLNLRGIVLPMLDPLQIKAWNSLGNWFKNEDGTAFETAHGKSYWDCVTEDPKDVEIFTETMASDSLLVAKFVITEHKSLFEGLDSIIDVGGSTGTFARAIAESFPSLKCTVLDLPETVANIEPTNNLEFVGGNMFEKIPPAHVVFLKMVLHDWNDEDCVKILKNCRKAIPEKEKGGKVILIETVVQGQMHDSESVKTQIAMDMDMLVSFGTKERTEKEWEILFKEAGFSSYKIFPIVDIRSLIEKIEMDLQTAEFREAQAKIWSQAFSFANCAALKCAVKLGIADAIDNHDKKALTLSELTEELSIKPSKSPFLQRLMRQLVNAGFFTEAKQLRDDNKDGRTTTAYALTPVSRLLLKNEQWNLRGIVLTMLDPAELKAWSVLNDWFKNDDPTAFQTAHEKNYWDYTAENTQHCQIFEDAMANDSVLVSKLLVTEYKFLFEGLTSLIDLGGSTGTIAKALAKSFPNLKCTVFDLPHVVANLESTKNLEFVGGDMFEKLPPSNAILLKWILHDWNDEDCVKILKNCKKAIQEKGNGGKVIIIDTVVYSQKNEKELVDLQISMDMAMVINFAAKERTEEEWEHLIREAGFSGHKIFPMYDFRSIIEVYP
>CRO_T104109
MDVQSDEFRKAHTYIWSQAFTFTGCASLKCAVELGIADAIHNHGKPMSLSDLTNTLEINPYKAQHLHRLMRFLANAGIFSEEAGTENVYSLTPLSRLLLKNARLNSRAFVLMMFDPLQMKAWNALSEWFKNDDQTAFETAHNGKSYWDFAVEEPRRLELFSEAMAADSLIVSEVLINKYKFFFEGLTSLVDVGGGTGELARTIAESFPTIKCSVLDLPEVVANVEKTENLEIIGGDMFENIPAANAIFLKMVLHDWNNENCVKILKNCKKAIPEKEKGGKVIILDTVVLGEVDDSESVKTQLCMDMDMMVLLGTNERSEKEWAAIIQEAGFSSYKIFPLLDIRSLIEVYP
>CRO_T104094
MEVQSVEFRKAQAHIWNQVFSFTTCASLKCAVQLGIADAIDDHGKPMSLFYLTKALQINPSKAPYIQRLMGILVNSGFFSQDKLTYYSLTPSSRLLLKKEALNLRAAVEIMLDPAELKAWSSLSDWFKDEKCRTAFEMVHGKNFWDFYAEESIHGEIFNRAMSTDSQLISKLLVTEYKFLFEGLTSLVDIGGGTGTIAKAIAKSFPIANLESKENLEFVGGDMFERIPNAKIVLHNWNDEDCVKKLKKCNEAIPGKEKSGKVIIIDILMDSKKEDYESLQAQISMDLQMMVLLDAKERREKEWAILFQKSGFSGYKIFSMFDYRSIIEHLENRLVVQAAEIKELVRHNRRLPATHVALGQDLVSSKEEIQRHVDHIKRTRTESDIHIRVSMDKIAKMEVGSIHYGVTFTAEGSSNLPRVTSAHWLKLLTIKNNTNATPSENLSGMKNLWILDSQCSHHMTSRKVFLRYLNNIYPYTIEKILGSPELGSEFSHTQGQKDNSPVPLYPGPVAACPCKHSAAHMQPTALTQHDSNCAAHSELPSPTARPNKNCPI
>CRO_T104091
MEVQSVEFCKAQAHIWNQVFSFTTCASLKCAVQLGIADAIDDHGKPMSLFYLTKALQINPSKAPYIQRLMGILVNSGFFSQDKLSYYSLTPSSRLLLKKEELNLRGAVEIMLDPAELKAWSSLSDWFKDEKCRTTFEMAHGKNFWDFCAEESIHGEIFNRAMSTDSQLISKLLVTEYKFLFEGLTSLVDIGGGTGTIAKAIAKSFPKLKCTVFDLPHVVANLESKENLEFVGGDMFERIPNANAILLKIVLHNWNDEDCMKILKKCKEAILGKEKSGKVIIIDIVMDSKKEDYESLQAQISMDLQMMVLLDAKERREKEWAILFQKSGFSGYKIFSMFDYRSIIEVYP
>CRO_T103893
MELQSSEIRNAQAHFFTQVFSFTSMSSLKCAVQLGIPDAIHSHGKPMALSDLTNSLPINPSKAPYIYRLMRILVAAGYFSEEEKNVYSLTPFTRLLLKNDPLNSISMVLGVNQIAELKAWNAMSEWFQNEDLTAFETAHGKNFWDFGAEDKYGKNFDGVMAADSILVSKMLIPEFNYLFEGLDSLVDVGGGTGTIAKAIAKSFPDLKCTVFDLPHVVANLESTENLEFVGGDMFEKIPSANAILLKWILHDWKDEECVKVLKMCRKAIPEKEKGGKVILIETVLMDSKKHENEEAVKAQISSDIDMMVFFTAKERTEEEWATLFREAGFSGYKIFPMIDFRSPIEVYP
>CRO_T103890
MDLETAEIRKAQAQYSRHVFSFVSTSCLKCAIQLEIPEAIHNHGKPMTLSDLTNSLPINPSKAPYIHRLMRILVAAGYFSEEPKNVYSLTSLSRILVKNQPLNLREFVLSANEIAEVEGWNALSEWFQNDVATAFQTAHGKTYWEYLSQDKYGKNFDQLMATDSLLISKLLIPDYNYLFEGLISLVDVGGGTGTLAGAVAKAFPNLKCTVFEQPHVIADLEAKGNLEFVGGDMFEKIPSANAILLKSVLHDWKDEDSVKILKNCKKAIPEKEKGGKVIVIDIVLMDSKKHDNPLVKSQISGDMDMMVSMGAKERTEEEWAALFKEAGFSGYKIFPMLDFRSPIEVYP
>CRO_T110596
MDVQSEEFREAQAQIWSQSCSFITSASLKCAVKLGIPDIIDNHGKPITLSELTNALVPPVHPSKAPFIYRLMRVLAKNGFCSEEQLDGETEPLYSLTLSSRILLKKEPLNLRGIVLTMADPVQLKAWESLSDWYQNEDDSSTAFEIAHGKNFWDYSSEHMEHAEFFNEAMASDSQLISKLLIGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLESKENVEFVAGDMFEKIPSANAIFLKWILHDWNDEDCVKILKSCKKAIPAKGGKVIVIDMVMYSDKKDDHLVKTQTSMDMAMLVNFAAKERCEKEWAFLFKEAGFSDYKIYPKLDFTRSLIEVYP
>CRO_T113068
MVGALSSSSGSNESSSHELLLKAHAHVWNMIYNFINSMSIKCAIDLNIPDIIHNHGNPITLSELTQSLKINSSKAPYVYRLMRILTHSGFFTKSKIGRNETEEVDAYALTPSSNLLLKDNPSNINPLLSLALQPTRIRAWLNLSDWFRNDEDLTRNPFANTHDGRDIWKMSGQEQGINNLFNEAMANDAWFISSLVVENFEIFKGLTSLVDVGGGTC
MEVQSAEFRKAQVHIWSQSFSFITCASLKCALQLGIADAIENHGKPMALSDLTNTLQINPSKSQYLPRLMRLLVNSGFFSEQQLGDAGGGENVYGLTPSMRLLLKNEPLNLRGIVLPMLDPLQIKAWNSLGNWFKNEDGTAFETAHGKSYWDCVTEDPKDVEIFTETMASDSLLVAKFVITEHKSLFEGLDSIIDVGGSTGTFARAIAESFPSLKCTVLDLPETVANIEPTNNLEFVGGNMFEKIPPAHVVFLKMVLHDWNDEDCVKILKNCRKAIPEKEKGGKVILIETVVQGQMHDSESVKTQIAMDMDMLVSFGTKERTEKEWEILFKEAGFSSYKIFPIVDIRSLIEKIEMDLQTAEFREAQAKIWSQAFSFANCAALKCAVKLGIADAIDNHDKKALTLSELTEELSIKPSKSPFLQRLMRQLVNAGFFTEAKQLRDDNKDGRTTTAYALTPVSRLLLKNEQWNLRGIVLTMLDPAELKAWSVLNDWFKNDDPTAFQTAHEKNYWDYTAENTQHCQIFEDAMANDSVLVSKLLVTEYKFLFEGLTSLIDLGGSTGTIAKALAKSFPNLKCTVFDLPHVVANLESTKNLEFVGGDMFEKLPPSNAILLKWILHDWNDEDCVKILKNCKKAIQEKGNGGKVIIIDTVVYSQKNEKELVDLQISMDMAMVINFAAKERTEEEWEHLIREAGFSGHKIFPMYDFRSIIEVYP
>CRO_T104109
MDVQSDEFRKAHTYIWSQAFTFTGCASLKCAVELGIADAIHNHGKPMSLSDLTNTLEINPYKAQHLHRLMRFLANAGIFSEEAGTENVYSLTPLSRLLLKNARLNSRAFVLMMFDPLQMKAWNALSEWFKNDDQTAFETAHNGKSYWDFAVEEPRRLELFSEAMAADSLIVSEVLINKYKFFFEGLTSLVDVGGGTGELARTIAESFPTIKCSVLDLPEVVANVEKTENLEIIGGDMFENIPAANAIFLKMVLHDWNNENCVKILKNCKKAIPEKEKGGKVIILDTVVLGEVDDSESVKTQLCMDMDMMVLLGTNERSEKEWAAIIQEAGFSSYKIFPLLDIRSLIEVYP
>CRO_T104094
MEVQSVEFRKAQAHIWNQVFSFTTCASLKCAVQLGIADAIDDHGKPMSLFYLTKALQINPSKAPYIQRLMGILVNSGFFSQDKLTYYSLTPSSRLLLKKEALNLRAAVEIMLDPAELKAWSSLSDWFKDEKCRTAFEMVHGKNFWDFYAEESIHGEIFNRAMSTDSQLISKLLVTEYKFLFEGLTSLVDIGGGTGTIAKAIAKSFPIANLESKENLEFVGGDMFERIPNAKIVLHNWNDEDCVKKLKKCNEAIPGKEKSGKVIIIDILMDSKKEDYESLQAQISMDLQMMVLLDAKERREKEWAILFQKSGFSGYKIFSMFDYRSIIEHLENRLVVQAAEIKELVRHNRRLPATHVALGQDLVSSKEEIQRHVDHIKRTRTESDIHIRVSMDKIAKMEVGSIHYGVTFTAEGSSNLPRVTSAHWLKLLTIKNNTNATPSENLSGMKNLWILDSQCSHHMTSRKVFLRYLNNIYPYTIEKILGSPELGSEFSHTQGQKDNSPVPLYPGPVAACPCKHSAAHMQPTALTQHDSNCAAHSELPSPTARPNKNCPI
>CRO_T104091
MEVQSVEFCKAQAHIWNQVFSFTTCASLKCAVQLGIADAIDDHGKPMSLFYLTKALQINPSKAPYIQRLMGILVNSGFFSQDKLSYYSLTPSSRLLLKKEELNLRGAVEIMLDPAELKAWSSLSDWFKDEKCRTTFEMAHGKNFWDFCAEESIHGEIFNRAMSTDSQLISKLLVTEYKFLFEGLTSLVDIGGGTGTIAKAIAKSFPKLKCTVFDLPHVVANLESKENLEFVGGDMFERIPNANAILLKIVLHNWNDEDCMKILKKCKEAILGKEKSGKVIIIDIVMDSKKEDYESLQAQISMDLQMMVLLDAKERREKEWAILFQKSGFSGYKIFSMFDYRSIIEVYP
>CRO_T103893
MELQSSEIRNAQAHFFTQVFSFTSMSSLKCAVQLGIPDAIHSHGKPMALSDLTNSLPINPSKAPYIYRLMRILVAAGYFSEEEKNVYSLTPFTRLLLKNDPLNSISMVLGVNQIAELKAWNAMSEWFQNEDLTAFETAHGKNFWDFGAEDKYGKNFDGVMAADSILVSKMLIPEFNYLFEGLDSLVDVGGGTGTIAKAIAKSFPDLKCTVFDLPHVVANLESTENLEFVGGDMFEKIPSANAILLKWILHDWKDEECVKVLKMCRKAIPEKEKGGKVILIETVLMDSKKHENEEAVKAQISSDIDMMVFFTAKERTEEEWATLFREAGFSGYKIFPMIDFRSPIEVYP
>CRO_T103890
MDLETAEIRKAQAQYSRHVFSFVSTSCLKCAIQLEIPEAIHNHGKPMTLSDLTNSLPINPSKAPYIHRLMRILVAAGYFSEEPKNVYSLTSLSRILVKNQPLNLREFVLSANEIAEVEGWNALSEWFQNDVATAFQTAHGKTYWEYLSQDKYGKNFDQLMATDSLLISKLLIPDYNYLFEGLISLVDVGGGTGTLAGAVAKAFPNLKCTVFEQPHVIADLEAKGNLEFVGGDMFEKIPSANAILLKSVLHDWKDEDSVKILKNCKKAIPEKEKGGKVIVIDIVLMDSKKHDNPLVKSQISGDMDMMVSMGAKERTEEEWAALFKEAGFSGYKIFPMLDFRSPIEVYP
>CRO_T110596
MDVQSEEFREAQAQIWSQSCSFITSASLKCAVKLGIPDIIDNHGKPITLSELTNALVPPVHPSKAPFIYRLMRVLAKNGFCSEEQLDGETEPLYSLTLSSRILLKKEPLNLRGIVLTMADPVQLKAWESLSDWYQNEDDSSTAFEIAHGKNFWDYSSEHMEHAEFFNEAMASDSQLISKLLIGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLESKENVEFVAGDMFEKIPSANAIFLKWILHDWNDEDCVKILKSCKKAIPAKGGKVIVIDMVMYSDKKDDHLVKTQTSMDMAMLVNFAAKERCEKEWAFLFKEAGFSDYKIYPKLDFTRSLIEVYP
>CRO_T113068
MVGALSSSSGSNESSSHELLLKAHAHVWNMIYNFINSMSIKCAIDLNIPDIIHNHGNPITLSELTQSLKINSSKAPYVYRLMRILTHSGFFTKSKIGRNETEEVDAYALTPSSNLLLKDNPSNINPLLSLALQPTRIRAWLNLSDWFRNDEDLTRNPFANTHDGRDIWKMSGQEQGINNLFNEAMANDAWFISSLVVENFEIFKGLTSLVDVGGGTC
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University