Enzyme
A cytochrome P-450 (heme thiolate) protein found in plants. The reaction involves the migration of the 2-phenyl group of the flavanone to the 3-position of the isoflavanone. The 2-hydroxyl group is derived from the oxygen molecule. EC 4.2.1.105, 2-hydroxyisoflavanone dehydratase, acts on the products with loss of water and formation of genistein and daidzein, respectively.
IFS2
Enzyme
2-hydroxyisoflavanone synthase
Enzyme name
EC:1.14.14.87
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Class
liquiritigenin, [reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (hydroxylating, aryl migration)
Sysname
(1) liquiritigenin + O2 + [reduced NADPH---hemoprotein reductase] = 2,4',7-trihydroxyisoflavanone + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07777];(2) (2S)-naringenin + O2 + [reduced NADPH---hemoprotein reductase] = 2,4',5,7-tetrahydroxyisoflavanone + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07778]
Reaction
2,4',7-trihydroxyisoflavanone [CPD:C15567];H2O [CPD:C00001];[oxidized NADPH---hemoprotein reductase] [CPD:C03161];2,4',5,7-tetrahydroxyisoflavanone [CPD:C12631]
Product
Species information
Fragaria vesca
Species name
Wild strawberry (Fragaria vesca) is a species of eudicot in the family Rosaceae (rose family).
Species info
Gene sequence info
Fragaria_vesca.IFS2.fa
Download
>rna-XM_004295903.2
MVAELGLLILFLVLSIFISKKFFVKHHGDLPPSPLALPIIGHFHLLSPLIHRSFHDLSLRFGPIFSLRLGSVPCIVVTSPELAKEFLKTHELSFINRSETTAVEKMTYNASFAFAPYGNYWKFTRKLITNELLGGRNVDNFLAIRNQEYMRFLRHLTKKAETCESINLSEELPRLGHNVIGQMMLGKYSSASRAEEMRVVVREATEIFGEFNFSDFNWIWKKFNLQGFQKRTEVTHKKFDVLVEKVISEREELRKMQKKGNITEEKDVKGFLDILLDIVEGESSENLDVEFSRDHIKGVITDLFAASIDTTSIAMDWTLAELINHPEVLKKAREEIDRVVGKGRLVGELDVPNLPYIQAIIKEAIRLHPPLTVVARKCVEQCKVGKYVIDKETMLFINNWAIGRDPQNWEKPLEFCPERFLQLDGGGDKASAIDVRGQHFQYLPFGSGRRICPGITLTMKMLPALLAAMIQCFNLKVVGTHCKKMDDETKVLEMDEKPGFTTPRAYDLVCVPVARFGPLSILKNT
>rna-XM_004299690.2
MVIELVLFILFLVLAFLISKHFRVNHLPPSPFALPIIGHFHLLSPLIHRSFHDLSRRLGPVFFLRLGSLPCVVVSSPELAKEFLKTHELSFINRSETTAVERMTYNASFAFAPYGPYWKFIRKLSTNELLSGRSVDKFLPIRNQEYMRLIRLLAKKAKACEPVNLSEELPRVGHNIIGQMMLGKYSSASRAEKARLVVREATKIFGEFNFSDFNGIWKKLDLQGFQKRIDATHHKFDALVEEFIAEREELRKKNIDGGNSTSAEEKDVKGFLDVLLDKVEECKSSTENGEVEFSRNNVKGLITDLFSASIDTTSISMEWTLAELINHPEVLKKAREEIDRVVETGRLVGESDLPNLPYIQAIMKETFRLHPPLAFVARKCTQQSKVGKYIIPENTMLFVNIWAMARDPKNWENPLEFRPERFLQLDADDSTNAIDIRGQHFQYLPFGSGRRICPGLPLTMKMLPTLLAIMIQCFDLKVSGAECKNTSNGKVLEMDERPGFTTPRASDLVCVPVSRFSSPIKYP
>rna-XM_004299044.2
METDFQGYIILFLVWLVSTILVRTILAKFRTNSRLPPSPMALPIIGHLHLLAPIPHQALHKLSNRLGPLVHIFLGSVPCVVVSSPEMAKEFLKTHEASFSNRPHMAAVDYLTYGSADFSFAPYGPYWKFMKKLCMSELLGGRTLDQLLPVRREEMTSFIDLMLNKAKANEAVDVGAELMNVTNNIISRMLMGQGCSASEKEADEVRKLVKAVAELTGKFNLSDFIWFCKNLDLQGFGKNLKKVRDQFDTMMERIIKEHQEALKKKKVAESDGYVKDLLDILLDISEDETSEIRLTRENIKAFILDIFAAGTDTSAITTEWALAELINHPDIMNKARQEINTIIGNNRLVQESDIANLPYLQAIVKETLRLHPTGPLIVRESTEPCSIGGYDIPARTRLFVNVWAVNRDPNHWEKPLEFEPERFTHNEGNGKSQLDVRGQHFHLLPFGSGRRGCPGTSLALQVVQTTLAAMIQCFEWKVPSGSVDMEEGLGLTLPRAHPLVCVPVARLSPFPSID
>rna-XM_011467431.1
MAIELVLFILFLVLVFLISRHFRVNHLLPPSPLALPIIGHFHLLSPLIHRSFHDLSLRLGPVYSLRLGSVPCVVVSSPEVAKEFLKTHELSFMNRTANIAAASMTYNASFAFASYGPYWKFVRKLSTNELLSSLSVDKFLPIRNQEYVRLLRLLAKKAEACEPVNLSEELPKVGHNIMGKMMLGKYSNDSRAARFRVVVRECTKIFGEFNFSDFNWIWKKFDVQGFQKRIDATHNKFDALVEEIIDEREELRKMKTNGGNNTGAEEKDVMGFLEVLLDKEEECKSTTKNFEVDFSRNHVKGLITVCNFHHSNLNPNVFSEFVNFQDMFTASIDTTSITMEWALAEVINHPEVLKKAREEMDRAVGTERLVGEWDLPNLPYIQAILKETLRLHPPLPIVPRICTEECKVGKYVFPEKTLLFVNTWAMGRDPKNWENPLEFRPERFLQLDGSDPANGTDVRGQHYHYLPFGSGRRICPGLTLTMKMLPTQVAVMIQCFDFKIGGTQCENTSNDKVLEMDEGPGLTVPRASDLVCFPIARFSSLLEYP
>rna-XM_011468298.1
MVWKKLDLQGFGKRIDDTHLKFDELVEKVVKEREQLRLKNKKTNGGEEKDEEVKDFLSILLDMLEDESGESAEVGFSRVHIKALITDLFTASTDTTAIAMEWALAELINHPAVLEKPREEIDRVVGNERIVEESDGSTLPYIQAIIKETFRLHPHVPMVTRISVEDYKVGEYVIPTNTILFVNNWAIGRDPKNWEKPLDFWPERFLEGGDQITGMDVRGQLFQFLPFGSERRICPGINLTMKMVPSLVAAMIQCFDWKVIPSKKMNNNDCVVEMDERPGLTTPRAHDLVCFPVACMHLLNILKP
>rna-XM_004305171.2
MALEVLLLFASGIISLLFFTRSVLSHTHRVQHLPPSPLALPIIGHFHILGPLIHRSFQALSFRFGPIFSLRLGSVPCIVVTSPELAKEFLKTHELSFATHAQSAAIKRITYDSSLAFSPYGPYWKFIKKVTVNELLSSRSVGKFHSIRADGYSRLLRLLAERAESCLAVNLSEELPRLGHNIIGQMMLGKYSTSSSTERAEEARLVVREATRIFGELNLCDFNMVWKKLDLQGFGKRIEDTHLKFDELVEKVVKEREQLRIKNKKTNGGEEKDEEVKDFLSILLDMLEDESGESVEVGFSRVHIKALIMDLFTASTDTTAITMEWALAELINHPAVLEKAREEIDRVVGNERIVEESDGSNLPYIQAIIKETFRLHPPVPMVTRISVEDCKVGEYVIPTNTILFVNNWAIGRDPKNWENPLDFRPERFLEGGDQITEMDVRGQHFQFLPFGSGRRICPGINLTMKMVPSLVAAMIQCFDWKVIPSKKMNNNDCVVEMDERPGLTTPRAHDLVCFPVARINLLNILKP
>rna-XM_004308261.2
MALELLLLFALGIISLLLFSRRALSHTHRVQHLLPPSPFALPIIGHFHLLGPLIHRSFQALSFRFGPIFSLRLGSVPCIVVTSPKLAKEFLKTHELSFVSRVQSAAIKRITYYSSLAFSPYGPYWKFIKKVTVNELLSSRSVSKFHSIRADEYSRLLRLLAERAKSCLAVNLSEELPRLCHNIIGQMMLGKYSTAASSSTERAEEARLVVREATRIFGELNLCDFNMVWKKLDLQGFGKRIEDTHLKFDELVEKVVMEREQLRIENNVQKTNGGEEKDEEVKDFLSILLDMLEDESGESVEVGFSRVHIKALITDLFTASTDTTAIAMEWALAELINHPAVLEKAREEIDRVVGNGRIVEESDGPNLPYIQAIIKETFRLHPPVPMVTRISVEDCKVGEYVIPTNTILFVNNWAIGRDSKNWEKPLDFWPERFLEGGDQTSGMDVRGQHFQFLPFGSGRRICPGINLTMKMVPSLVGAMIQCFDWKVIPSKKMNNIDGVVEMDERPGLTTPRAHDLVCFPVARFNLLNILKP
>rna-XM_004309429.2
MSTLFHSTNMANCHEYLVLLLIWFVSFILFRTIFTKTQTHHRLPPSPRALPIIGHLHLLGRIPHQALHKLSTRHGPLIQLFFGSIPCVVVSSPEMARECLKTNEISFLNRPKRTNVDYLTYGSADFVMAPYGPYWKFMKKLCMSELLGGRVLDQNLPIRQEERERFLHFMLKKAGKGEQVDVGGELLRLTNNIISLMVFNRRFSDDDEEAHEVREVLEEMCELAGRFNFSDMFWFCKNLDLQGYAKRLKDVRDRYDKMMGKIIEEHEEARRNKKNDPWKGDNAVNLLDILLDIYEDQSSEIKLTRENIKGFIMNMFGAGTDTSSVTIEWALSELINRPDVMAKAREEIDSVVGTNRLVSESDVVNLPYLQAIVKETLRLHPTGPMIVRESTQHCTIDGYDIPAKTRILVNVWAINRDPKHWKDPLEFMPERFWKSTLDVRGQSFQFMPFGSGRRSCPGASLALHVVHTTLASVIQCFGWKVGDGAAGIDGRVDMEEGPGISLPRAKPLVCVPSVRFSPFPLS
MVAELGLLILFLVLSIFISKKFFVKHHGDLPPSPLALPIIGHFHLLSPLIHRSFHDLSLRFGPIFSLRLGSVPCIVVTSPELAKEFLKTHELSFINRSETTAVEKMTYNASFAFAPYGNYWKFTRKLITNELLGGRNVDNFLAIRNQEYMRFLRHLTKKAETCESINLSEELPRLGHNVIGQMMLGKYSSASRAEEMRVVVREATEIFGEFNFSDFNWIWKKFNLQGFQKRTEVTHKKFDVLVEKVISEREELRKMQKKGNITEEKDVKGFLDILLDIVEGESSENLDVEFSRDHIKGVITDLFAASIDTTSIAMDWTLAELINHPEVLKKAREEIDRVVGKGRLVGELDVPNLPYIQAIIKEAIRLHPPLTVVARKCVEQCKVGKYVIDKETMLFINNWAIGRDPQNWEKPLEFCPERFLQLDGGGDKASAIDVRGQHFQYLPFGSGRRICPGITLTMKMLPALLAAMIQCFNLKVVGTHCKKMDDETKVLEMDEKPGFTTPRAYDLVCVPVARFGPLSILKNT
>rna-XM_004299690.2
MVIELVLFILFLVLAFLISKHFRVNHLPPSPFALPIIGHFHLLSPLIHRSFHDLSRRLGPVFFLRLGSLPCVVVSSPELAKEFLKTHELSFINRSETTAVERMTYNASFAFAPYGPYWKFIRKLSTNELLSGRSVDKFLPIRNQEYMRLIRLLAKKAKACEPVNLSEELPRVGHNIIGQMMLGKYSSASRAEKARLVVREATKIFGEFNFSDFNGIWKKLDLQGFQKRIDATHHKFDALVEEFIAEREELRKKNIDGGNSTSAEEKDVKGFLDVLLDKVEECKSSTENGEVEFSRNNVKGLITDLFSASIDTTSISMEWTLAELINHPEVLKKAREEIDRVVETGRLVGESDLPNLPYIQAIMKETFRLHPPLAFVARKCTQQSKVGKYIIPENTMLFVNIWAMARDPKNWENPLEFRPERFLQLDADDSTNAIDIRGQHFQYLPFGSGRRICPGLPLTMKMLPTLLAIMIQCFDLKVSGAECKNTSNGKVLEMDERPGFTTPRASDLVCVPVSRFSSPIKYP
>rna-XM_004299044.2
METDFQGYIILFLVWLVSTILVRTILAKFRTNSRLPPSPMALPIIGHLHLLAPIPHQALHKLSNRLGPLVHIFLGSVPCVVVSSPEMAKEFLKTHEASFSNRPHMAAVDYLTYGSADFSFAPYGPYWKFMKKLCMSELLGGRTLDQLLPVRREEMTSFIDLMLNKAKANEAVDVGAELMNVTNNIISRMLMGQGCSASEKEADEVRKLVKAVAELTGKFNLSDFIWFCKNLDLQGFGKNLKKVRDQFDTMMERIIKEHQEALKKKKVAESDGYVKDLLDILLDISEDETSEIRLTRENIKAFILDIFAAGTDTSAITTEWALAELINHPDIMNKARQEINTIIGNNRLVQESDIANLPYLQAIVKETLRLHPTGPLIVRESTEPCSIGGYDIPARTRLFVNVWAVNRDPNHWEKPLEFEPERFTHNEGNGKSQLDVRGQHFHLLPFGSGRRGCPGTSLALQVVQTTLAAMIQCFEWKVPSGSVDMEEGLGLTLPRAHPLVCVPVARLSPFPSID
>rna-XM_011467431.1
MAIELVLFILFLVLVFLISRHFRVNHLLPPSPLALPIIGHFHLLSPLIHRSFHDLSLRLGPVYSLRLGSVPCVVVSSPEVAKEFLKTHELSFMNRTANIAAASMTYNASFAFASYGPYWKFVRKLSTNELLSSLSVDKFLPIRNQEYVRLLRLLAKKAEACEPVNLSEELPKVGHNIMGKMMLGKYSNDSRAARFRVVVRECTKIFGEFNFSDFNWIWKKFDVQGFQKRIDATHNKFDALVEEIIDEREELRKMKTNGGNNTGAEEKDVMGFLEVLLDKEEECKSTTKNFEVDFSRNHVKGLITVCNFHHSNLNPNVFSEFVNFQDMFTASIDTTSITMEWALAEVINHPEVLKKAREEMDRAVGTERLVGEWDLPNLPYIQAILKETLRLHPPLPIVPRICTEECKVGKYVFPEKTLLFVNTWAMGRDPKNWENPLEFRPERFLQLDGSDPANGTDVRGQHYHYLPFGSGRRICPGLTLTMKMLPTQVAVMIQCFDFKIGGTQCENTSNDKVLEMDEGPGLTVPRASDLVCFPIARFSSLLEYP
>rna-XM_011468298.1
MVWKKLDLQGFGKRIDDTHLKFDELVEKVVKEREQLRLKNKKTNGGEEKDEEVKDFLSILLDMLEDESGESAEVGFSRVHIKALITDLFTASTDTTAIAMEWALAELINHPAVLEKPREEIDRVVGNERIVEESDGSTLPYIQAIIKETFRLHPHVPMVTRISVEDYKVGEYVIPTNTILFVNNWAIGRDPKNWEKPLDFWPERFLEGGDQITGMDVRGQLFQFLPFGSERRICPGINLTMKMVPSLVAAMIQCFDWKVIPSKKMNNNDCVVEMDERPGLTTPRAHDLVCFPVACMHLLNILKP
>rna-XM_004305171.2
MALEVLLLFASGIISLLFFTRSVLSHTHRVQHLPPSPLALPIIGHFHILGPLIHRSFQALSFRFGPIFSLRLGSVPCIVVTSPELAKEFLKTHELSFATHAQSAAIKRITYDSSLAFSPYGPYWKFIKKVTVNELLSSRSVGKFHSIRADGYSRLLRLLAERAESCLAVNLSEELPRLGHNIIGQMMLGKYSTSSSTERAEEARLVVREATRIFGELNLCDFNMVWKKLDLQGFGKRIEDTHLKFDELVEKVVKEREQLRIKNKKTNGGEEKDEEVKDFLSILLDMLEDESGESVEVGFSRVHIKALIMDLFTASTDTTAITMEWALAELINHPAVLEKAREEIDRVVGNERIVEESDGSNLPYIQAIIKETFRLHPPVPMVTRISVEDCKVGEYVIPTNTILFVNNWAIGRDPKNWENPLDFRPERFLEGGDQITEMDVRGQHFQFLPFGSGRRICPGINLTMKMVPSLVAAMIQCFDWKVIPSKKMNNNDCVVEMDERPGLTTPRAHDLVCFPVARINLLNILKP
>rna-XM_004308261.2
MALELLLLFALGIISLLLFSRRALSHTHRVQHLLPPSPFALPIIGHFHLLGPLIHRSFQALSFRFGPIFSLRLGSVPCIVVTSPKLAKEFLKTHELSFVSRVQSAAIKRITYYSSLAFSPYGPYWKFIKKVTVNELLSSRSVSKFHSIRADEYSRLLRLLAERAKSCLAVNLSEELPRLCHNIIGQMMLGKYSTAASSSTERAEEARLVVREATRIFGELNLCDFNMVWKKLDLQGFGKRIEDTHLKFDELVEKVVMEREQLRIENNVQKTNGGEEKDEEVKDFLSILLDMLEDESGESVEVGFSRVHIKALITDLFTASTDTTAIAMEWALAELINHPAVLEKAREEIDRVVGNGRIVEESDGPNLPYIQAIIKETFRLHPPVPMVTRISVEDCKVGEYVIPTNTILFVNNWAIGRDSKNWEKPLDFWPERFLEGGDQTSGMDVRGQHFQFLPFGSGRRICPGINLTMKMVPSLVGAMIQCFDWKVIPSKKMNNIDGVVEMDERPGLTTPRAHDLVCFPVARFNLLNILKP
>rna-XM_004309429.2
MSTLFHSTNMANCHEYLVLLLIWFVSFILFRTIFTKTQTHHRLPPSPRALPIIGHLHLLGRIPHQALHKLSTRHGPLIQLFFGSIPCVVVSSPEMARECLKTNEISFLNRPKRTNVDYLTYGSADFVMAPYGPYWKFMKKLCMSELLGGRVLDQNLPIRQEERERFLHFMLKKAGKGEQVDVGGELLRLTNNIISLMVFNRRFSDDDEEAHEVREVLEEMCELAGRFNFSDMFWFCKNLDLQGYAKRLKDVRDRYDKMMGKIIEEHEEARRNKKNDPWKGDNAVNLLDILLDIYEDQSSEIKLTRENIKGFIMNMFGAGTDTSSVTIEWALSELINRPDVMAKAREEIDSVVGTNRLVSESDVVNLPYLQAIVKETLRLHPTGPMIVRESTQHCTIDGYDIPAKTRILVNVWAINRDPKHWKDPLEFMPERFWKSTLDVRGQSFQFMPFGSGRRSCPGASLALHVVHTTLASVIQCFGWKVGDGAAGIDGRVDMEEGPGISLPRAKPLVCVPSVRFSPFPLS
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University