Enzyme
A cytochrome P-450 (heme thiolate) protein found in plants. The reaction involves the migration of the 2-phenyl group of the flavanone to the 3-position of the isoflavanone. The 2-hydroxyl group is derived from the oxygen molecule. EC 4.2.1.105, 2-hydroxyisoflavanone dehydratase, acts on the products with loss of water and formation of genistein and daidzein, respectively.
IFS2
Enzyme
2-hydroxyisoflavanone synthase
Enzyme name
EC:1.14.14.87
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Class
liquiritigenin, [reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (hydroxylating, aryl migration)
Sysname
(1) liquiritigenin + O2 + [reduced NADPH---hemoprotein reductase] = 2,4',7-trihydroxyisoflavanone + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07777];(2) (2S)-naringenin + O2 + [reduced NADPH---hemoprotein reductase] = 2,4',5,7-tetrahydroxyisoflavanone + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07778]
Reaction
2,4',7-trihydroxyisoflavanone [CPD:C15567];H2O [CPD:C00001];[oxidized NADPH---hemoprotein reductase] [CPD:C03161];2,4',5,7-tetrahydroxyisoflavanone [CPD:C12631]
Product
Species information
Ficus erecta
Species name
Ai xiao tian xian guo (Ficus erecta) is a species of eudicot in the family Moraceae (mulberry family).
Species info
Gene sequence info
Ficus_erecta.IFS2.fa
Download
>Fer1.1_chr00_g075650.1
MSQFKLFGYDVYPKSILQVNVWAIGRDPDYWDKPEEFFPERFMDSSIDYKGQHFELLPFGAGRRGCPGIHMGMAMVELTLANLLCCFDGNYLMG
>Fer_r1.1chr02_g023060.1
MVALELPTQLLFLLLALVPLVLLIPVFRRHGSHHRLPPSPVGLPIIGHFHLLGPLLHRSFRDLSDRLGPLVLHQPRLRPLHRGLLAGARQGVPKDQRALVRRPRGLPGGKPPHLRLLHGLCPI
>Fer_r1.1chr02_g023070.1
MIALDELPTQLPLLLLALVLLIPIFHRHGRRHRLPPSPVGMPIIGHFHLLGPLLHRSFHDLSLRLGPLFTLRLGSVPCVVASTPDLARELLKTNELSFTSRPSFMAVSRLTYGTAMVSSPYDAYWKFLRRITTNDLLGSRPINGFSRLRSRELSRFLRVLADKSSRHEAANLSAELRKMSYAVILHMMLGGDSAAASHGMDEGRVEEARTVVRDVTRIFGEFNLSDFVWFLKRLDLQGFGKRIEDIFRRYDTLIETVLREREELRRNRKDGVGNIDFLDILLDYTEDPKDDNVQFTRLHIKGLIMDIFTAGTDTTATSIEWTLSELINNPTELEKVREEIDRVVGETRLVSESDVQNLPYLQAVVKEALRLHPPAPLVTRKCVKECKVGEYTIPVDTMLIVNTWTIGRDPNHWESPLDFQPNRFLEAHSGMDVKGQHFELLPFGSGRRMCPGLNSALQILPLVVAAIIQCFDLKVVRSIGDSKDGDGDGDGDGPVLNMEEGVGLTLPRLHELVCVPVARHSTLTAILESEL
>Fer_r1.1chr05_g006020.1
MENLLQLFLVNFMPFLLLMFIILKVASGKRSANSTHCLPPGPWRLPVIGNLHLLIGYDLPHHRLRDLEKKYGPPYASTAWRTF
>Fer_r1.1chr10_g008200.1
MIALELLPPLLLLLSLVVLIPIFRHRHRGLPPSPVGMPIIGHFHLLGPLLHRSFRDLSLRWGPLFSIRLGSVPCIVASSPDLAREFLKTNELSFASRNDSIAVARLTYGSSIAFSPYDAYWKFIRRLTMNDLLSSRPLSGFISLRSREVHRFLRVLGDKSSRREAVNLSADLRKLSYAIISQMILGDNDTSSSTSAGRVEEARTVVREVTKIFGEFNLSDFVWCLRRLDFQGFGKRIEEIFRRYDALIETVLREREELRRQRKNKDKEKDTDKNKDKEYRDFLDILLDHMEDQKEDNVPFTRLHVKGLIMDIFTAGTDTTATSTEWTLSELINNRTVLEKAREEIDRVVGRTRLVTEADTPNLPYIQAVVKEAFRLHPAVPLVTRKCVRECRVGEHKIPVGAMLVVNNWAIGRDPHYWECPSDFRPERFLQSHGEGSNSSMLDIRGQHFELLPFGTGRRICPGMNLALHMLPLVVATIIQCFDLKVVGSLDDENDGDGHVLKMEEAPGLTVPRLHELICVPSARLTSVTGILAPEMIE
>Fer_r1.1chr12_g021550.1
MADLQDFLFLLFLALASITLLVRAIFFSKRRTKSLLRLPPSPPALPIIGHLHLLRKLPHQALCKVADHYGPIVYLQFGYKHCLLVSSAEIARECLKTNESCFLNRPQRANLDYLTYGGADFVVAPYGPYWKFMKKLTVSKLLGASIMEVYKNIRRQEIGLFVQTILKKAEEGKEIDVGAQLVGLTNNIISRMALKQKCAKTEDEAYEIRHVLDDMCDLAGKLNVQDFIWFFRRFDPQRFGKRLKEVRDRYDNLMGKIIREHEDAKRKRMESDIDDGEVKDILDMLLDVYEDENSEIKLTKENIQAYVMNIFGAGTETQSVTMEWALSELITHPNAMSKARQEVDKVVGNNRLVQESDIPNLPYIQAVVKETLRLHPGGPFIARESSKDCTINGYHVPANTRLFLNTWAINRDPRHWENPHEFLPERFLGEDGNLKSDLDVRGQHFQLLPFGSGRRGCPGVNLALLVVPTTVAALVHCFDWKVGKDGNGVVDMEEGFDGLSLPMAKPLVGFPVARLNPFPKVI
>Fer_r1.1chr12_g021540.1
MANPQDLLFLLFLALASTTLLVRAVFFSKRKTKSLLRLPPSPPALPIIGHLHLLGKLPHQDLCKVSDRYGPIVYLRFGYKRSLLVSSAEIARECLKTNESCFLNRPQRANFDYLTYGGADFSVAPYAPYWKFMRKLTVSKLLGASIMEVYKTIRREEIGQLVQTILKKAEEGKEIDVGAQLMGLTNNIISRMALKQKCAKTEDEAYEMRHVVDEMCDLAGKLNVQDFVWFCRNFDLQRFGKRLKEVRDRYDNLMGKIIREHEEAKRKRMEGDIDDGEVKDILDMLLDVYEDENSEIKLTKENIHAYVMNIFGAGTETQSVTMEWALSELMTHPNAMSKARQEVDKIIGSNKLVQESDIPNLPYIQAVVKETLRLHPGGPFIARESSEDCTINGYHVPANTRLFLNTWAINRDPRHWENPHEFLPERFLGEDGNLKSDLDVRGQHFQLLPFGSGRRGCPGVNLALLVVPTTVAALVHCFDWKVGEDGNGVVDMEEGFGLSLPRAKPLVCFPVARLNPFPKVI
MSQFKLFGYDVYPKSILQVNVWAIGRDPDYWDKPEEFFPERFMDSSIDYKGQHFELLPFGAGRRGCPGIHMGMAMVELTLANLLCCFDGNYLMG
>Fer_r1.1chr02_g023060.1
MVALELPTQLLFLLLALVPLVLLIPVFRRHGSHHRLPPSPVGLPIIGHFHLLGPLLHRSFRDLSDRLGPLVLHQPRLRPLHRGLLAGARQGVPKDQRALVRRPRGLPGGKPPHLRLLHGLCPI
>Fer_r1.1chr02_g023070.1
MIALDELPTQLPLLLLALVLLIPIFHRHGRRHRLPPSPVGMPIIGHFHLLGPLLHRSFHDLSLRLGPLFTLRLGSVPCVVASTPDLARELLKTNELSFTSRPSFMAVSRLTYGTAMVSSPYDAYWKFLRRITTNDLLGSRPINGFSRLRSRELSRFLRVLADKSSRHEAANLSAELRKMSYAVILHMMLGGDSAAASHGMDEGRVEEARTVVRDVTRIFGEFNLSDFVWFLKRLDLQGFGKRIEDIFRRYDTLIETVLREREELRRNRKDGVGNIDFLDILLDYTEDPKDDNVQFTRLHIKGLIMDIFTAGTDTTATSIEWTLSELINNPTELEKVREEIDRVVGETRLVSESDVQNLPYLQAVVKEALRLHPPAPLVTRKCVKECKVGEYTIPVDTMLIVNTWTIGRDPNHWESPLDFQPNRFLEAHSGMDVKGQHFELLPFGSGRRMCPGLNSALQILPLVVAAIIQCFDLKVVRSIGDSKDGDGDGDGDGPVLNMEEGVGLTLPRLHELVCVPVARHSTLTAILESEL
>Fer_r1.1chr05_g006020.1
MENLLQLFLVNFMPFLLLMFIILKVASGKRSANSTHCLPPGPWRLPVIGNLHLLIGYDLPHHRLRDLEKKYGPPYASTAWRTF
>Fer_r1.1chr10_g008200.1
MIALELLPPLLLLLSLVVLIPIFRHRHRGLPPSPVGMPIIGHFHLLGPLLHRSFRDLSLRWGPLFSIRLGSVPCIVASSPDLAREFLKTNELSFASRNDSIAVARLTYGSSIAFSPYDAYWKFIRRLTMNDLLSSRPLSGFISLRSREVHRFLRVLGDKSSRREAVNLSADLRKLSYAIISQMILGDNDTSSSTSAGRVEEARTVVREVTKIFGEFNLSDFVWCLRRLDFQGFGKRIEEIFRRYDALIETVLREREELRRQRKNKDKEKDTDKNKDKEYRDFLDILLDHMEDQKEDNVPFTRLHVKGLIMDIFTAGTDTTATSTEWTLSELINNRTVLEKAREEIDRVVGRTRLVTEADTPNLPYIQAVVKEAFRLHPAVPLVTRKCVRECRVGEHKIPVGAMLVVNNWAIGRDPHYWECPSDFRPERFLQSHGEGSNSSMLDIRGQHFELLPFGTGRRICPGMNLALHMLPLVVATIIQCFDLKVVGSLDDENDGDGHVLKMEEAPGLTVPRLHELICVPSARLTSVTGILAPEMIE
>Fer_r1.1chr12_g021550.1
MADLQDFLFLLFLALASITLLVRAIFFSKRRTKSLLRLPPSPPALPIIGHLHLLRKLPHQALCKVADHYGPIVYLQFGYKHCLLVSSAEIARECLKTNESCFLNRPQRANLDYLTYGGADFVVAPYGPYWKFMKKLTVSKLLGASIMEVYKNIRRQEIGLFVQTILKKAEEGKEIDVGAQLVGLTNNIISRMALKQKCAKTEDEAYEIRHVLDDMCDLAGKLNVQDFIWFFRRFDPQRFGKRLKEVRDRYDNLMGKIIREHEDAKRKRMESDIDDGEVKDILDMLLDVYEDENSEIKLTKENIQAYVMNIFGAGTETQSVTMEWALSELITHPNAMSKARQEVDKVVGNNRLVQESDIPNLPYIQAVVKETLRLHPGGPFIARESSKDCTINGYHVPANTRLFLNTWAINRDPRHWENPHEFLPERFLGEDGNLKSDLDVRGQHFQLLPFGSGRRGCPGVNLALLVVPTTVAALVHCFDWKVGKDGNGVVDMEEGFDGLSLPMAKPLVGFPVARLNPFPKVI
>Fer_r1.1chr12_g021540.1
MANPQDLLFLLFLALASTTLLVRAVFFSKRKTKSLLRLPPSPPALPIIGHLHLLGKLPHQDLCKVSDRYGPIVYLRFGYKRSLLVSSAEIARECLKTNESCFLNRPQRANFDYLTYGGADFSVAPYAPYWKFMRKLTVSKLLGASIMEVYKTIRREEIGQLVQTILKKAEEGKEIDVGAQLMGLTNNIISRMALKQKCAKTEDEAYEMRHVVDEMCDLAGKLNVQDFVWFCRNFDLQRFGKRLKEVRDRYDNLMGKIIREHEEAKRKRMEGDIDDGEVKDILDMLLDVYEDENSEIKLTKENIHAYVMNIFGAGTETQSVTMEWALSELMTHPNAMSKARQEVDKIIGSNKLVQESDIPNLPYIQAVVKETLRLHPGGPFIARESSEDCTINGYHVPANTRLFLNTWAINRDPRHWENPHEFLPERFLGEDGNLKSDLDVRGQHFQLLPFGSGRRGCPGVNLALLVVPTTVAALVHCFDWKVGEDGNGVVDMEEGFGLSLPRAKPLVCFPVARLNPFPKVI
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University