Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Fagopyrum esculentum
Species name
Common buckwheat (Fagopyrum esculentum) is a species of eudicot in the family Polygonaceae (buckwheat family).
Species info
Gene sequence info
Fagopyrum_esculentum.FLS.fa
Download
>gene:Fes_sc0001994.1.g000009.aua.1
MIRRSDQMIRGDPVIRGYPMIRGYPGFPDIHGYPFLSGYSKPKTPIRIRSGDPEKSPGSDRIRTGSKRILSNGKYKAVLHRSPVSKEKARMSWPVFLEPPSEHLVGEYVYCKLNKLPQ
>gene:Fes_sc0006344.1.g000007.aua.1
MGGDDIEVEMKINMYPPCPQPELALGVEPHTDMSALTVLVPNDVLGLQCWKDGNWISVDYFPDGIYIHVGDQIEVLSNGMYKSILHRSLVNKEKTRMSWAVFCVPPGQKTVGPLPELINEENPAKYTAKTFDEYRYRKINKLPQ
>gene:Fes_sc0008841.1.g000003.gia.1
MNVVIQVLSNGMYKSILHRSLVNKEKTRMSWAVFCVPPGQKTVGPLPELVNEENPAKYTAKTFDEYRYRKINKLPQ
>gene:Fes_sc0014821.1.g000002.aua.1
MVYVYEKAKGTRIILLRDVIDEFTLMSNEIVKKLLRAMPLSLNLEEKKFVEQYGEEGLMFGRYNYYPPCPRADELLGKLRVTQKLSNNSENTQKKLSFPQERSKENLFTQASRIRNEKEIRNIHKDQGKERGKQRRKQFTRTGDTAKETAGAAGQAQGRMTTGAAQTFDSNTSRLNSKEPENNDTSKTRNQQESRFKLDSWRRRKEEREGENERKKERKKHAGIHERKEEQRMMNLHVQQDHRGARERLQLLGLKTNVRNGKKKEKTGIYRDGLER
>gene:Fes_sc0017610.1.g000004.aua.1
MEVVRVQAIASLSMREESIPCEYIRSEKEQPAKTSVEGSFLEVPAIDISEADEKLAEMFYKASSEWGLFQVVNHGIPSEVISRLQRAGKDFFDLPQEEKERYAKADDSKDIEGYGTKLQKEVDGKKGWVDHLFHRIWPPSVINYNFWPTNPPSYREANEEYCKYLREVAEKIMKSLSKGLGLEEEDLKEAAGGDSLEYLLKINYYPPCPRPDLALGVVAHTDMSALTILVPNDVPGLQVFRDGLWYDVGYIRDALIIHVGDQLEILSNGKYKAVLHRSTVSKEKARMSWPVFLEPPSEHLIGPHPKLIDDNNPANFKTKKFGEYVYCKLNKLPQ
>gene:Fes_sc0022076.1.g000003.aua.1
MATTIRGGERRPHQNYNHLHNDDLTTFAPPPSPMPTAGVPRPSAPLSVDISINEYLTSNLQIPVLQRLSDPLPPPTSTSTTTTTTSSFVHLPCLPAQIDFRLLASGVGNSIERMMKSAKEFGMFRMTGHGISREELSAARAVVGEFFTRLDERKNKFRRSFVERIGNREEFVWFLSDKAMTLWAPW
>gene:Fes_sc0035730.1.g000001.aua.1
MFGMEANEEYCKCLRGVAEKIFRCMSLGLGLEGPELMEAAGGDSLEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPGLQVFRDGFWYDVKYIPNALIVHVGDQLEIMSNGKYKAVLHRTTVNKEKVRMSWPVFLEPPAEHVLGPHPKIVDDRNPAKYKTKKYGDYMYCKLNKLPQ
>gene:Fes_sc0063661.1.g000001.aua.1
MATTIRGGERRPHQNYNHLHNDDLTTFAPPPSPMPTAGVPRPSAPLSAVDISINEYLTSNLQIPVLQRLSDPLPPPTSTSTTTTTTSSFVHLPCLPAQIDFRLLASGVGNSIERMMKSAKEFGMFRMTGHGISREELSAARAVVGEFFTRLDERKNKFRRSFVERIGNREEFVWFLSDKAMTLWAREV
MIRRSDQMIRGDPVIRGYPMIRGYPGFPDIHGYPFLSGYSKPKTPIRIRSGDPEKSPGSDRIRTGSKRILSNGKYKAVLHRSPVSKEKARMSWPVFLEPPSEHLVGEYVYCKLNKLPQ
>gene:Fes_sc0006344.1.g000007.aua.1
MGGDDIEVEMKINMYPPCPQPELALGVEPHTDMSALTVLVPNDVLGLQCWKDGNWISVDYFPDGIYIHVGDQIEVLSNGMYKSILHRSLVNKEKTRMSWAVFCVPPGQKTVGPLPELINEENPAKYTAKTFDEYRYRKINKLPQ
>gene:Fes_sc0008841.1.g000003.gia.1
MNVVIQVLSNGMYKSILHRSLVNKEKTRMSWAVFCVPPGQKTVGPLPELVNEENPAKYTAKTFDEYRYRKINKLPQ
>gene:Fes_sc0014821.1.g000002.aua.1
MVYVYEKAKGTRIILLRDVIDEFTLMSNEIVKKLLRAMPLSLNLEEKKFVEQYGEEGLMFGRYNYYPPCPRADELLGKLRVTQKLSNNSENTQKKLSFPQERSKENLFTQASRIRNEKEIRNIHKDQGKERGKQRRKQFTRTGDTAKETAGAAGQAQGRMTTGAAQTFDSNTSRLNSKEPENNDTSKTRNQQESRFKLDSWRRRKEEREGENERKKERKKHAGIHERKEEQRMMNLHVQQDHRGARERLQLLGLKTNVRNGKKKEKTGIYRDGLER
>gene:Fes_sc0017610.1.g000004.aua.1
MEVVRVQAIASLSMREESIPCEYIRSEKEQPAKTSVEGSFLEVPAIDISEADEKLAEMFYKASSEWGLFQVVNHGIPSEVISRLQRAGKDFFDLPQEEKERYAKADDSKDIEGYGTKLQKEVDGKKGWVDHLFHRIWPPSVINYNFWPTNPPSYREANEEYCKYLREVAEKIMKSLSKGLGLEEEDLKEAAGGDSLEYLLKINYYPPCPRPDLALGVVAHTDMSALTILVPNDVPGLQVFRDGLWYDVGYIRDALIIHVGDQLEILSNGKYKAVLHRSTVSKEKARMSWPVFLEPPSEHLIGPHPKLIDDNNPANFKTKKFGEYVYCKLNKLPQ
>gene:Fes_sc0022076.1.g000003.aua.1
MATTIRGGERRPHQNYNHLHNDDLTTFAPPPSPMPTAGVPRPSAPLSVDISINEYLTSNLQIPVLQRLSDPLPPPTSTSTTTTTTSSFVHLPCLPAQIDFRLLASGVGNSIERMMKSAKEFGMFRMTGHGISREELSAARAVVGEFFTRLDERKNKFRRSFVERIGNREEFVWFLSDKAMTLWAPW
>gene:Fes_sc0035730.1.g000001.aua.1
MFGMEANEEYCKCLRGVAEKIFRCMSLGLGLEGPELMEAAGGDSLEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPGLQVFRDGFWYDVKYIPNALIVHVGDQLEIMSNGKYKAVLHRTTVNKEKVRMSWPVFLEPPAEHVLGPHPKIVDDRNPAKYKTKKYGDYMYCKLNKLPQ
>gene:Fes_sc0063661.1.g000001.aua.1
MATTIRGGERRPHQNYNHLHNDDLTTFAPPPSPMPTAGVPRPSAPLSAVDISINEYLTSNLQIPVLQRLSDPLPPPTSTSTTTTTTSSFVHLPCLPAQIDFRLLASGVGNSIERMMKSAKEFGMFRMTGHGISREELSAARAVVGEFFTRLDERKNKFRRSFVERIGNREEFVWFLSDKAMTLWAREV
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University