Enzyme
A cytochrome P-450 (heme thiolate) protein found in plants. The reaction involves the migration of the 2-phenyl group of the flavanone to the 3-position of the isoflavanone. The 2-hydroxyl group is derived from the oxygen molecule. EC 4.2.1.105, 2-hydroxyisoflavanone dehydratase, acts on the products with loss of water and formation of genistein and daidzein, respectively.
IFS2
Enzyme
2-hydroxyisoflavanone synthase
Enzyme name
EC:1.14.14.87
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Class
liquiritigenin, [reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (hydroxylating, aryl migration)
Sysname
(1) liquiritigenin + O2 + [reduced NADPH---hemoprotein reductase] = 2,4',7-trihydroxyisoflavanone + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07777];(2) (2S)-naringenin + O2 + [reduced NADPH---hemoprotein reductase] = 2,4',5,7-tetrahydroxyisoflavanone + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07778]
Reaction
2,4',7-trihydroxyisoflavanone [CPD:C15567];H2O [CPD:C00001];[oxidized NADPH---hemoprotein reductase] [CPD:C03161];2,4',5,7-tetrahydroxyisoflavanone [CPD:C12631]
Product
Species information
Eriobotrya japonica
Species name
Loquat (Rhaphiolepis bibas) is a species of eudicot in the family Rosaceae (rose family).
Species info
Gene sequence info
Eriobotrya_japonica.IFS2.fa
Download
>EVM0004336.1
MAVLELLSLFSFLFIVSLLLRPLFASKSQTLLPPSPVTLPILGHFHLLSPLIHQTFYKLSLRFGPLFSLRLGSVPCVIISSPDLAKEFLKTHERSFISRSQSIAIERLSYNSSFAFGPYGPYYKFIRKLSMNEFLSSRSVSNFASIRTEEYLRLLTLLAKKAESGEAINLSKELPKLSNNVIAKMMLGNSKGSSAEGIDEEVGLLGREVTRIFGEFNLSDFVWFCKKLDLQGFGKRIEDTHRRFDALVEKVISEREQLRKKNMKNEGGSGGEEVKDFLDILLDILEKGSAESEEVEFTRVHIKALILDFFSAGIDTTAISMEWALAELINHPKVLEKAREEIDRVVGNRRLVLESDGPNLPYIQAIIKETLRLHPSVPVIPRKSVQKCNIGNYVIPENTMLFVNAWAIGRDPKYWENPLHFYPERFLAPLSGGGDGARALDVRGQHFQLLPFGTGRRMCPGLNLALQMLPALLGAMVQCFDWKVVGVNQKHMNGDDRVLEMDERPGLTTPRAHDLICIPVARFDPEWYP
>EVM0021627.1
MALDQVLFMILFLLLTIPLILFPTLTSKLFQSHHLRLPPSPLALPIIGHYHLLGPLLHRTFHNLSHRFGPLFSLWLGSLQCSSAAPHELSFISHGQSLAIESMTYNASLAFAPYGPYWKFVKKLTVNKLLSNHSIHNSMPLRTQEYLKLLRLLAKKAESCEAVNLTEEFPKLWNNVTVDMILGTKGSSAEGKATWVEEARLVVATRIFGEMSLCDFFWVCKKLDLGGFVKRIGDTHKRFDVLVEKIIRELEEMRNKEKEEHVGEEKEEVKDFLDILVDMLKDESLEVEFTSVHLKALITDLFTAGTDTNTIALEWALAELINHPRVLEKAREEIYRVIGNKRLVGELDVPNLPYLQAIIKETFRLHPPVGGYDIPTNTMLFVNVWDVGRDPKIWDCPLDFWPERFLQQGTDNQVNSVDVRGRVCPGVNLTTKMLQGVLTAMIQCFDWKVVGSSALRRKTLSTRTIHHFHYLLCWSP
>EVM0016081.1
MAVLELLFLFSVLLTIPFLLRPVFASKSETLLPPSPLALPIIGHFYLLGPLLHRSFYKLSLRFGPLFSLRLGSVPCIVVTSPDIAKEFLKTHELSFISRAQTIAIERLTYNYSTPGFAPYGPYYKLIKKLSVNEFLSNRNVSNFGSIRTQEYLSLLRLLAKKAESGEAVNLTEELPKLFNNVIAKMMLGNSKGSSAEGRDEEARLVAREVTRIFGEFNLSDFVWFCKKLDLQGFGKRIENTRRRYDALVEKVISEREELRKKNMKNGGGNVEEEMVMKDFLDLLLDLLEKKSGEIEGVKFTRVHIKALIMGFFTAGTDATAILFEWALAELINHPKVLKKVREEIDRVVGNKRLLVESDGPNLPYIQAVIKEACRLHPPVSVVTRKSVEKCKFGKYVIPENTMLFVNVWAIGRDPKYWENPLVFQPERFLSPPLSGGRDGTSAFDVRGQHFELLPFGTGRRICPGVNLVMQMLPTLLGAMVQCFDWKVIGPQQKMNGNDLILEMDERPGMTSPRAHDLVCIPVARLNLLDVLDP
>EVM0037321.1
MPVPDLLFLFSVLFIIYLILRLVFTSKSQTLLPPSPFALPIIGHFHLIGPLIQRSFYNLSLRFGPLFSICLGSVLGILVSSPEIVKEFLKTHELSFISHTESFATKRLTYNASLALAPYGPYWKFIKKLSINEFLSSRSVNNFASILTQEYLRLLRMLAKKAESGEAVNLTEELPKLSNNVIAKMILGSSKGPSDEGRDEEARVVVRKKIDLQGFGKRIEDTHRRYDALVEKVISERDELRKKNVRNGGVSGGQEEVEEVKGFLDILLDLLKKGGGEIEDVNFTRVHIKALIMWALAELINHPKVLNKAREEIDIVVGNGRLLVESNGPNLPYIQAIIKETLRLHPPMSLITRKCVQQCKIGKYVMPENTVLFVSAWAIGRDPKYWEHPLDFQPEIFLSRLGEGGWTSTIDVRGQHFQLIPFGSGRRICPGLNLALQMLPGLLGAMIQCFDFEVVEPDRKNTNGDGRVLEMDERPGMTTPRANDLVCILVARLSSLCVL
>EVM0018463.1
MAVLELLFLFSILLTLPLFLRLVFTSKSETLLPPSPFALPIIGHFHLLRPLLHRSFYKLSLGFGPLISLRLGSIPCIVVTSPEIAKEFLKTHELSFISRAQTIAIERLTYNYSTTGFAPYGPYYKLIKKLSVNEVLGNRNVSNFASIRTQEHLRLSRILAKKAESGEAVNLTEELPKLFNNVIAKMMLGNSKVTRIFGEFNLSNFVWFCKKLDLQEFGKRIEDTRRIYDALVEKVISEREELRKKNMKNGGGNVEEEMELKDFLDLLLDFDFFTAGTDTTAILIEWVLAELINHPKVLKKVREEIDRVVGNKRLLVESDGPNLPYIQAVIKEAFRLHPPVSVVTRKFNKCVEKCKFGKYVIPENTMVFVNVWTIGRDPKYWENPLDFQRERFLSPPHSGGDGTSALDVRGKHFELLPFGTGRRICPGVNLVMQMLPALLGAMVQCFDWKVIGPHQKKMNGNNLILELDERPGVTTPRTHDLVCIPAARFNLLDVLCP
>EVM0015738.1
MEVDFQGYFILFLVWLVSTVLVRTMLTKFRTKPRLPPSPMALPILGHLHLLAPIPHQALQKLSNRYGSLIHLFLGSVPCVVASTPEMAKEFLKTHEASFSNRPYISAVDYLTYGSADFSFAPYGPYWKFMKKLCMSELLGGRTLDQLLPVRREELASFVKLLLNKAKAAEPVDVGAELMTVTNNVISRMIMGQSCSSNEMEADEIRKQVKAIAELTGKFNLSDFIWFCKNLDLQGFGKQLKEVRDRFDVMMERIIKEHQEERKKKEVGEGGGDVVKDLLDILLDIAEDETSEFRLTRENIKAFILDIFSAGTDTSAITTEWALAELINHPDVMQKARQEIDTIIGTNRLVQESDIANLPYLQAIVKETLRLHPTGPLIVRESSEFCTIGGYDIPAKTRLFVNVWAINRDPSHWERPLEFKPERFVTEEGSGKSQLDVRGQHFHLLPFGSGRRGCPGTSLAMHVVQTTLAAMVQCFEWKVEGTVDMEEAAGLTLPRAHPLVCVPVARFNPVPSS
>EVM0013609.1
MAVVELLSLFSVLFVVFLLLRPLSASKSQTLLPPSPFALPIIGHFHLLSPLIHQSFYKLSLRLGPLFSLRLGSVPCIVISSPDLAKESLKTHELSFISRSQSIAIERLTYNSSLAFGPYGPYHKFIKKLSLNEFLSSRSVSNFALIRTQEYLRLLRLLAKKAESGEAVNLTEELPKLSNNVIAKMMLGNSKGSSSAEGRDEEARLVVREVTRIFGEFNLSDFVWFCKKLDLQGFGKRIEHTHRRFDVLVEKVISEREELRKKNMKNEGGSGGEEVKDFLDILLDILEKASAESEEVEFTRVHIKALIMDFFTAGTDTTAISMEWALAELINHPKVLEKAREEIDRVVGNIRLVVESDGPNLPYIQAIIKETLRLHPSVPMIPRKSVQKCNIGNYVIPENTMLFVNAWAIGRDPKYWENPLHFYPERFLAPLSGGGDGASALDVRGQHFQLLPFGTGRRICPGLNLALQMLPALLGAMVQCFDWKVVGVNQKHMNGDDRVLEMDERPGMTTPRAHDLICIPVARFDTKLYT
>EVM0011338.1
MIKSLLSDMFVASTETSTSTVEWGMAEILKNPDVHKKIILELDQVVGKDRFVEESDTVNLPYLQAAVKEVFRLHPAVPLITRRSYEACEVSGYHFPKDCVAVVNIWGMARDQSIWEERCKFRVKPESISPCL
MAVLELLSLFSFLFIVSLLLRPLFASKSQTLLPPSPVTLPILGHFHLLSPLIHQTFYKLSLRFGPLFSLRLGSVPCVIISSPDLAKEFLKTHERSFISRSQSIAIERLSYNSSFAFGPYGPYYKFIRKLSMNEFLSSRSVSNFASIRTEEYLRLLTLLAKKAESGEAINLSKELPKLSNNVIAKMMLGNSKGSSAEGIDEEVGLLGREVTRIFGEFNLSDFVWFCKKLDLQGFGKRIEDTHRRFDALVEKVISEREQLRKKNMKNEGGSGGEEVKDFLDILLDILEKGSAESEEVEFTRVHIKALILDFFSAGIDTTAISMEWALAELINHPKVLEKAREEIDRVVGNRRLVLESDGPNLPYIQAIIKETLRLHPSVPVIPRKSVQKCNIGNYVIPENTMLFVNAWAIGRDPKYWENPLHFYPERFLAPLSGGGDGARALDVRGQHFQLLPFGTGRRMCPGLNLALQMLPALLGAMVQCFDWKVVGVNQKHMNGDDRVLEMDERPGLTTPRAHDLICIPVARFDPEWYP
>EVM0021627.1
MALDQVLFMILFLLLTIPLILFPTLTSKLFQSHHLRLPPSPLALPIIGHYHLLGPLLHRTFHNLSHRFGPLFSLWLGSLQCSSAAPHELSFISHGQSLAIESMTYNASLAFAPYGPYWKFVKKLTVNKLLSNHSIHNSMPLRTQEYLKLLRLLAKKAESCEAVNLTEEFPKLWNNVTVDMILGTKGSSAEGKATWVEEARLVVATRIFGEMSLCDFFWVCKKLDLGGFVKRIGDTHKRFDVLVEKIIRELEEMRNKEKEEHVGEEKEEVKDFLDILVDMLKDESLEVEFTSVHLKALITDLFTAGTDTNTIALEWALAELINHPRVLEKAREEIYRVIGNKRLVGELDVPNLPYLQAIIKETFRLHPPVGGYDIPTNTMLFVNVWDVGRDPKIWDCPLDFWPERFLQQGTDNQVNSVDVRGRVCPGVNLTTKMLQGVLTAMIQCFDWKVVGSSALRRKTLSTRTIHHFHYLLCWSP
>EVM0016081.1
MAVLELLFLFSVLLTIPFLLRPVFASKSETLLPPSPLALPIIGHFYLLGPLLHRSFYKLSLRFGPLFSLRLGSVPCIVVTSPDIAKEFLKTHELSFISRAQTIAIERLTYNYSTPGFAPYGPYYKLIKKLSVNEFLSNRNVSNFGSIRTQEYLSLLRLLAKKAESGEAVNLTEELPKLFNNVIAKMMLGNSKGSSAEGRDEEARLVAREVTRIFGEFNLSDFVWFCKKLDLQGFGKRIENTRRRYDALVEKVISEREELRKKNMKNGGGNVEEEMVMKDFLDLLLDLLEKKSGEIEGVKFTRVHIKALIMGFFTAGTDATAILFEWALAELINHPKVLKKVREEIDRVVGNKRLLVESDGPNLPYIQAVIKEACRLHPPVSVVTRKSVEKCKFGKYVIPENTMLFVNVWAIGRDPKYWENPLVFQPERFLSPPLSGGRDGTSAFDVRGQHFELLPFGTGRRICPGVNLVMQMLPTLLGAMVQCFDWKVIGPQQKMNGNDLILEMDERPGMTSPRAHDLVCIPVARLNLLDVLDP
>EVM0037321.1
MPVPDLLFLFSVLFIIYLILRLVFTSKSQTLLPPSPFALPIIGHFHLIGPLIQRSFYNLSLRFGPLFSICLGSVLGILVSSPEIVKEFLKTHELSFISHTESFATKRLTYNASLALAPYGPYWKFIKKLSINEFLSSRSVNNFASILTQEYLRLLRMLAKKAESGEAVNLTEELPKLSNNVIAKMILGSSKGPSDEGRDEEARVVVRKKIDLQGFGKRIEDTHRRYDALVEKVISERDELRKKNVRNGGVSGGQEEVEEVKGFLDILLDLLKKGGGEIEDVNFTRVHIKALIMWALAELINHPKVLNKAREEIDIVVGNGRLLVESNGPNLPYIQAIIKETLRLHPPMSLITRKCVQQCKIGKYVMPENTVLFVSAWAIGRDPKYWEHPLDFQPEIFLSRLGEGGWTSTIDVRGQHFQLIPFGSGRRICPGLNLALQMLPGLLGAMIQCFDFEVVEPDRKNTNGDGRVLEMDERPGMTTPRANDLVCILVARLSSLCVL
>EVM0018463.1
MAVLELLFLFSILLTLPLFLRLVFTSKSETLLPPSPFALPIIGHFHLLRPLLHRSFYKLSLGFGPLISLRLGSIPCIVVTSPEIAKEFLKTHELSFISRAQTIAIERLTYNYSTTGFAPYGPYYKLIKKLSVNEVLGNRNVSNFASIRTQEHLRLSRILAKKAESGEAVNLTEELPKLFNNVIAKMMLGNSKVTRIFGEFNLSNFVWFCKKLDLQEFGKRIEDTRRIYDALVEKVISEREELRKKNMKNGGGNVEEEMELKDFLDLLLDFDFFTAGTDTTAILIEWVLAELINHPKVLKKVREEIDRVVGNKRLLVESDGPNLPYIQAVIKEAFRLHPPVSVVTRKFNKCVEKCKFGKYVIPENTMVFVNVWTIGRDPKYWENPLDFQRERFLSPPHSGGDGTSALDVRGKHFELLPFGTGRRICPGVNLVMQMLPALLGAMVQCFDWKVIGPHQKKMNGNNLILELDERPGVTTPRTHDLVCIPAARFNLLDVLCP
>EVM0015738.1
MEVDFQGYFILFLVWLVSTVLVRTMLTKFRTKPRLPPSPMALPILGHLHLLAPIPHQALQKLSNRYGSLIHLFLGSVPCVVASTPEMAKEFLKTHEASFSNRPYISAVDYLTYGSADFSFAPYGPYWKFMKKLCMSELLGGRTLDQLLPVRREELASFVKLLLNKAKAAEPVDVGAELMTVTNNVISRMIMGQSCSSNEMEADEIRKQVKAIAELTGKFNLSDFIWFCKNLDLQGFGKQLKEVRDRFDVMMERIIKEHQEERKKKEVGEGGGDVVKDLLDILLDIAEDETSEFRLTRENIKAFILDIFSAGTDTSAITTEWALAELINHPDVMQKARQEIDTIIGTNRLVQESDIANLPYLQAIVKETLRLHPTGPLIVRESSEFCTIGGYDIPAKTRLFVNVWAINRDPSHWERPLEFKPERFVTEEGSGKSQLDVRGQHFHLLPFGSGRRGCPGTSLAMHVVQTTLAAMVQCFEWKVEGTVDMEEAAGLTLPRAHPLVCVPVARFNPVPSS
>EVM0013609.1
MAVVELLSLFSVLFVVFLLLRPLSASKSQTLLPPSPFALPIIGHFHLLSPLIHQSFYKLSLRLGPLFSLRLGSVPCIVISSPDLAKESLKTHELSFISRSQSIAIERLTYNSSLAFGPYGPYHKFIKKLSLNEFLSSRSVSNFALIRTQEYLRLLRLLAKKAESGEAVNLTEELPKLSNNVIAKMMLGNSKGSSSAEGRDEEARLVVREVTRIFGEFNLSDFVWFCKKLDLQGFGKRIEHTHRRFDVLVEKVISEREELRKKNMKNEGGSGGEEVKDFLDILLDILEKASAESEEVEFTRVHIKALIMDFFTAGTDTTAISMEWALAELINHPKVLEKAREEIDRVVGNIRLVVESDGPNLPYIQAIIKETLRLHPSVPMIPRKSVQKCNIGNYVIPENTMLFVNAWAIGRDPKYWENPLHFYPERFLAPLSGGGDGASALDVRGQHFQLLPFGTGRRICPGLNLALQMLPALLGAMVQCFDWKVVGVNQKHMNGDDRVLEMDERPGMTTPRAHDLICIPVARFDTKLYT
>EVM0011338.1
MIKSLLSDMFVASTETSTSTVEWGMAEILKNPDVHKKIILELDQVVGKDRFVEESDTVNLPYLQAAVKEVFRLHPAVPLITRRSYEACEVSGYHFPKDCVAVVNIWGMARDQSIWEERCKFRVKPESISPCL
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University