Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Alnus glutinosa
Species name
Alnus glutinosa is a species of eudicot in the family Betulaceae (birch family).
Species info
Gene sequence info
Alnus_glutinosa.ANR.fa
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>Alngl11271S14071
MRHCLNRPTLGMLLMLNRKKNWITMEENSNSRVCVTGASGYIGSWLAKKLLEKGYTVHATLRNLGDTSKVDLLKSLPNADNRLVLFQADIYNPSDFEPAIKGCEYVFHVATPMQHDTQSSQYKDTAEAAVAGVRTIADSCIRSQTVKRLIYTASLMASLPLTEDGASFKSCIDESCWTPLLALDDISFTYANDSLLGYTKSKTLAEKEVLKYNEYENGRLEVVTLACGLVGGETLLPYVPTSVEVILSPLTSNLSWFNAFKFLQEVWGSIALVHIEDVCQAHIFCMEKPSMKGRFLCAATNPTISEIAVYFQEKFPEYAIAKEFLEGPNQGVVRCDSTKLMKMGFDYVYDENKILDDSVACGRRCGALM
>Alngl13974S16158
MEKHGDRVAMEKNSCKVCVTGGAGFIGSSLGKKLLHKGYTVHATLRNLAAIAGAKSIAMCCGRAGTVRRLIYTASVVSASPLKHDGSGFKDLMDETCWTPLNLSFAYCNDSLKDYTDSKTMAEKEILRYGDNNNGDGSLEVVSLPCGLVGGNTLLSSIPSSAAILISQLTNMANEQRTLNILEELLGKIPVVHIDDVCEAHIFCMEKDSINGRFLCGSSYVSSPEIANHCRQHYPDQFHVMQEYLDRPERKIEWGSKKLIEKGFVYKCDLKMIIDESIESARRLGYL
>Alngl41972S26960
MSSGAGKIVCVTGASGYIASWLVKFLLQRGYAVRASVHDPKKTNHLLELDGAKERLHLFKANLLEEGSFNSAIEGCECVFHTASPFYNDAKDPQAELLDPAIKGTLNVLNSCAKSPSVKRVVLTSSIAAVAYNGRPRTPDVVVDETWFSDPEVCKKANKMSSGAGKIACVTGASGYIASWLVKFLLQRGYAVRASVRDPNDPKKTNHLLDLDGAKERLHLFKANLLEEGSFNSAIEGCECVFHTASPVLLSHDSAIKDPQAELLDPAVKGTLNVLNSCANSPSVKRVVLTSSTAAVAFNGRPRTPDVVVDETWFSDPEVCKQANQWYVVSKTLAEDAAWKFAKEKGIDLVAINPSMVIGPLLQPTLNSSAAVISNLINGAQVFPNLTFGWVDVRDVAKAHIQACEIPSASGRYCIVETVAHNSEVVRTLRAQVFPNLTFGWVDVRDVAKAHIQAYEIPSASGRYCVVGRVAHYSEVVRTLRELYPSLRLPEKCADDKPFVPTYQVSKERAKSLGIEFTPFEVSLKDTVESLKEKNFNAIAMYVIKKFKQCETP
>Alngl135167S15767
MLLIKMEENIISINRRVCVTGASGYIGSWLAKKLLEKGYTVHATLRNLGQTSKVDLLKSLPNADNRLVLFEADIYNPTDFEPAIKGCEYVFHVATPMQHDTQSSQYKDTAEAAVAGVRTIADSCIRSQTVKRLIYTASLMASSPLTEDGASFKSCIDESCWTPFHDISFTYANDFLLGYTKSKTLAEKEFLKYNEYENGRLEVVTLACGLVGGETLLPYVPSSVEVILSPLTSNLSLFNALKFLQEVWGSIALVHIEDVCQAHIFCMEKPSMKGRFLCAATNPTITEIAIYLQEKFPEYEIAKEFFEGPDQGVVRCDFTKLMKMGFEYAYDEKKILDDSVECGRRCGVLL
>Alngl425579S28806
MVHRGQASPDQEEGFTYVISGASFQSWTAFGLRCLASHAELIDSAVKGTINVLRSCTKVPSVKRVIVTSSMVAVAYNGKPLTFDVSWYVVSKTLAEEAAWRFAKENEIDLVTINPRFVIGPFLQPTLNFTTEEILKLIDGAQTFPNPFYRFVDIRDVANAHIQALEAPSATGRYCLVETIIHCSERMSGAKKVVCVTAASGYIASWLVKLLLQRGYTVKATSELIDPAVMGTLNGSCAKVASIKRVIVTSSMASVIRSRRLLAPDVVVDRDLVFRSSYLWYQLSKTLAEEAAWKFAEECGIDLVAINPGFSIGPLIQPTPTSTIKGFLELMNGTLLDEDDKPLRPLHKVSQDKAKSLGISFIPLENPYLLYVISKSSNCFKAKHSQREAERERERERARMSGAEKVVCVTGASGYIASWVVKILLQRGYTVKATVRDPNDRKKTEFLLALDGAKERLQLFKASLLEEGCFDSVVDGCQGVFHIASPSEIIDPAVKGTLNVLRSCAKVPSIKRVIITSSMASVMFNRKPVTPDVVVDETWFSDPVLCKEEKLWYHLSKTLAEEAAWKFAEECGIDLVAINPGFVIGPFIQPTPTFSVEGFLKLVNEFAFINQSHGLHSSKISKKFHAFIIFGRFEDDKPLVPVYKISQDKAKSLGISFTPLEVSIGDTIESLKEKGFLTGSHASCYQPVQFGKNKIFGIFSFNTERETERARMSGAEKVVCVTGASGYIASWVVKILLQRGYTVKATVRDPNDRKKTEFLLALDGAKERLQLFKASLLEEGCFDSVVDGCQGVFHIASPVFFTANDPQSEIIDPAVKGTLNVLRSCAKVPSIKRVIITSSMASVMFNRKPVTPDVVVDETWFSDPVLCKEEKHNVSHASSSIQIVSVVTLLSLSISMPASWFFLLFLVIILCQIFSDVIIADSYKFYFQLWYHLSKTLAEEAAWKFAEECGIDLVAINPGFVIGPFIQPTPTFSVEGFLKXQPIFHG
MRHCLNRPTLGMLLMLNRKKNWITMEENSNSRVCVTGASGYIGSWLAKKLLEKGYTVHATLRNLGDTSKVDLLKSLPNADNRLVLFQADIYNPSDFEPAIKGCEYVFHVATPMQHDTQSSQYKDTAEAAVAGVRTIADSCIRSQTVKRLIYTASLMASLPLTEDGASFKSCIDESCWTPLLALDDISFTYANDSLLGYTKSKTLAEKEVLKYNEYENGRLEVVTLACGLVGGETLLPYVPTSVEVILSPLTSNLSWFNAFKFLQEVWGSIALVHIEDVCQAHIFCMEKPSMKGRFLCAATNPTISEIAVYFQEKFPEYAIAKEFLEGPNQGVVRCDSTKLMKMGFDYVYDENKILDDSVACGRRCGALM
>Alngl13974S16158
MEKHGDRVAMEKNSCKVCVTGGAGFIGSSLGKKLLHKGYTVHATLRNLAAIAGAKSIAMCCGRAGTVRRLIYTASVVSASPLKHDGSGFKDLMDETCWTPLNLSFAYCNDSLKDYTDSKTMAEKEILRYGDNNNGDGSLEVVSLPCGLVGGNTLLSSIPSSAAILISQLTNMANEQRTLNILEELLGKIPVVHIDDVCEAHIFCMEKDSINGRFLCGSSYVSSPEIANHCRQHYPDQFHVMQEYLDRPERKIEWGSKKLIEKGFVYKCDLKMIIDESIESARRLGYL
>Alngl41972S26960
MSSGAGKIVCVTGASGYIASWLVKFLLQRGYAVRASVHDPKKTNHLLELDGAKERLHLFKANLLEEGSFNSAIEGCECVFHTASPFYNDAKDPQAELLDPAIKGTLNVLNSCAKSPSVKRVVLTSSIAAVAYNGRPRTPDVVVDETWFSDPEVCKKANKMSSGAGKIACVTGASGYIASWLVKFLLQRGYAVRASVRDPNDPKKTNHLLDLDGAKERLHLFKANLLEEGSFNSAIEGCECVFHTASPVLLSHDSAIKDPQAELLDPAVKGTLNVLNSCANSPSVKRVVLTSSTAAVAFNGRPRTPDVVVDETWFSDPEVCKQANQWYVVSKTLAEDAAWKFAKEKGIDLVAINPSMVIGPLLQPTLNSSAAVISNLINGAQVFPNLTFGWVDVRDVAKAHIQACEIPSASGRYCIVETVAHNSEVVRTLRAQVFPNLTFGWVDVRDVAKAHIQAYEIPSASGRYCVVGRVAHYSEVVRTLRELYPSLRLPEKCADDKPFVPTYQVSKERAKSLGIEFTPFEVSLKDTVESLKEKNFNAIAMYVIKKFKQCETP
>Alngl135167S15767
MLLIKMEENIISINRRVCVTGASGYIGSWLAKKLLEKGYTVHATLRNLGQTSKVDLLKSLPNADNRLVLFEADIYNPTDFEPAIKGCEYVFHVATPMQHDTQSSQYKDTAEAAVAGVRTIADSCIRSQTVKRLIYTASLMASSPLTEDGASFKSCIDESCWTPFHDISFTYANDFLLGYTKSKTLAEKEFLKYNEYENGRLEVVTLACGLVGGETLLPYVPSSVEVILSPLTSNLSLFNALKFLQEVWGSIALVHIEDVCQAHIFCMEKPSMKGRFLCAATNPTITEIAIYLQEKFPEYEIAKEFFEGPDQGVVRCDFTKLMKMGFEYAYDEKKILDDSVECGRRCGVLL
>Alngl425579S28806
MVHRGQASPDQEEGFTYVISGASFQSWTAFGLRCLASHAELIDSAVKGTINVLRSCTKVPSVKRVIVTSSMVAVAYNGKPLTFDVSWYVVSKTLAEEAAWRFAKENEIDLVTINPRFVIGPFLQPTLNFTTEEILKLIDGAQTFPNPFYRFVDIRDVANAHIQALEAPSATGRYCLVETIIHCSERMSGAKKVVCVTAASGYIASWLVKLLLQRGYTVKATSELIDPAVMGTLNGSCAKVASIKRVIVTSSMASVIRSRRLLAPDVVVDRDLVFRSSYLWYQLSKTLAEEAAWKFAEECGIDLVAINPGFSIGPLIQPTPTSTIKGFLELMNGTLLDEDDKPLRPLHKVSQDKAKSLGISFIPLENPYLLYVISKSSNCFKAKHSQREAERERERERARMSGAEKVVCVTGASGYIASWVVKILLQRGYTVKATVRDPNDRKKTEFLLALDGAKERLQLFKASLLEEGCFDSVVDGCQGVFHIASPSEIIDPAVKGTLNVLRSCAKVPSIKRVIITSSMASVMFNRKPVTPDVVVDETWFSDPVLCKEEKLWYHLSKTLAEEAAWKFAEECGIDLVAINPGFVIGPFIQPTPTFSVEGFLKLVNEFAFINQSHGLHSSKISKKFHAFIIFGRFEDDKPLVPVYKISQDKAKSLGISFTPLEVSIGDTIESLKEKGFLTGSHASCYQPVQFGKNKIFGIFSFNTERETERARMSGAEKVVCVTGASGYIASWVVKILLQRGYTVKATVRDPNDRKKTEFLLALDGAKERLQLFKASLLEEGCFDSVVDGCQGVFHIASPVFFTANDPQSEIIDPAVKGTLNVLRSCAKVPSIKRVIITSSMASVMFNRKPVTPDVVVDETWFSDPVLCKEEKHNVSHASSSIQIVSVVTLLSLSISMPASWFFLLFLVIILCQIFSDVIIADSYKFYFQLWYHLSKTLAEEAAWKFAEECGIDLVAINPGFVIGPFIQPTPTFSVEGFLKXQPIFHG
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University