Enzyme
Isolated from the plants Silene dioica (red campion) [1], Iris ensata (Japanese iris) [2] and Iris hollandica (Dutch iris) [3]. Also acts on the 3-O-rutinosides of pelargonidin, delphinidin and malvidin, but not the corresponding glucosides or 6-acylglucosides. The enzyme does not catalyse the glucosylation of the 5-hydroxy group of cyanidin 3-glucoside.
5GT
Enzyme
Cyanidin 3-O-rutinoside 5-O-glucosyltransferase
Enzyme name
EC:2.4.1.116
Entry
Transferases;Glycosyltransferases;Hexosyltransferases
Class
UDP-alpha-D-glucose:cyanidin-3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside 5-O-beta-D-glucosyltransferase
Sysname
UDP-alpha-D-glucose + cyanidin-3-O-rutinoside = UDP + cyanidin 3-O-rutinoside 5-O-beta-D-glucoside [RN:R06819]
Reaction
UDP [CPD:C00015];cyanidin 3-O-rutinoside 5-O-beta-D-glucoside [CPD:C12646]
Product
Species information
Eragrostis curvula
Species name
Weeping love grass (Eragrostis curvula) is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Eragrostis_curvula.5GT.fa
Download
>rna-gnl|WGS:RWGY|EJB05_41433.mrna
MSPRPHFLVLTFPFQGHIAPALRLARRLLAAAPDALVTFSTTDIAHRRMFPAKDDAAAERGNDDDDGRLEFLPFSDGTEAGYVPSSDKGAFTAYMDSFHAEGARSVGELLDALAARGRPVSRVVYTLLLPWAADVARERGVPSALYWIQPAAVFAIYHHYFHGHAGVVDEHRHDPTFVVQLPGLPPQAIEDLPSFITQSTDPSDPLHSCFTTLRDLFDALDRETPKATVLVNTCEELEVGALAAVGAYDVLPVGPVLPSGDEVGLFKRDDAKYMEWLDSKPANSVVYVSFGSLAAMEREQLDELLLGLEESGRPYLCVVRKDIKASIFKQGRTEVIFGFTMRNNRV
>rna-gnl|WGS:RWGY|EJB05_41425.mrna
MESPPPPPPQHFLFVTNPMQGHINPARRLAARVLASTPGARVTFSTAVSGHRRMFPDLASPDEEAVDAAGVLHLPYSDSFDEGFNPAVHGLGSYKARAPAAGRETLSAVVARLAERGRPVTCLVYTFLVPWAADVARAHGVAAALYWIQPAAVFAVYYHYFHGHDAAVLAACANVSEPVQLPGLPPLGPDALPSVVFITSPEHRHYLLLDLLRDLFRSLDEHKPKVLVNTFDALEAEELRAVPQFELAAVGPVVPDDASSPSNSDLFHRHDATAYMEWLDTKPARSVVYVSFGSVLSMSARQEEEMRRGLEATGRPYLWVARKPADACAGNSDGGDGAQGMVVEWCDQVRVLSHPAVGCFVTHCGWNSTMESVTRGVPMVAVPQWTDQPTVAWLVEARMGAGVRARVDGEGVVERRELQRCVEAVMGDGDAAAAVREQAALWSKQAGEAVACGGTSERNLGAFASFRPAVDNA
>rna-gnl|WGS:RWGY|EJB05_41432.mrna
MQSHINPARRLAARVAAALPNARVTFSTAVSGHCQMFPHLTSPDGEVIQGLVSYIPYSDGFDCGFSPCGEAAGGRVCAYRARARAVGAETLAAVFARLAARGRPVTRVVYTALVGWVPGVARAHGVPAALYWVKPATVFAVYYHYFHGHGEVLHSIANNETDPDDASVHLPGLPPLKPDALPAFVSMASPGSRQYLTLDMLRDIFFALDEHMPTVLVDTFDALEYEALRALPQFDLVAVGPVVVEEPCRPCVALFQPNDAKAYMDWLDKKSARSVVFVSLGSVLSVSKLQDEELRRGLEATGRPYLLVTHKINSGGVAGDGGQGMVVEWCNQMGVLAHPAVGCFVTHCRWDSTLESITCGVPMVAVPRWADQPTVAALVETSVGVGVRAQVDGEGVLTRGELQRCVEMVMGSTHSAAAVRARAERWGQSAKEAAAAGGTSERSLRAFATGL
>rna-gnl|WGS:RWGY|EJB05_41411.mrna
MDWLDKKPARSVVFVSLGSVLSVSKRLDEQMRRGLEATGRPYLLVARKSSGGGVAGTGGQGMVVEWCNQMRVLAHPAVGCFVS
>rna-gnl|WGS:RWGY|EJB05_44821.mrna
MASPADDPCVKAQQHFLFVTGPLHGHINPVQRLAARVLAANPGARVTFSTAVSGHRAPAHIPVPRAAQRGDGGVLHAPYSDGYDDGFDPSAHDARLYRARTRDADRASLAAVVARLRPTVTRVAYTFLVAWALDVARDAGVPAALYWIQPATVFAVYHRVLHGGGHGDALAAACAGGGSVELPGLPALTADALPSIVSAASPEHPMHGAFQSFRDLFVDLDENRPRVLVNTFEALEPEALRAVPELEVVAVGPAVPDEASLSPRTTDDTTEDYMAWLDTKAARSVVYVSFGSFGAAGRGDEARPGCHRPAVPMGATKQDDEKEQEGMVVAWCEQVRVLSHPAVGCFVTHCGWNSALESMACGVPIVALPHWTDQPTVAWLLAERAGVGVRACAVAGDGVVESGELQRCVEAVMGDGQCAADIFSMNLEFRI
>rna-gnl|WGS:RWGY|EJB05_50925.mrna
MADADNHHSEYGGRGDSHFLVVANGMQGHLNPARALARRLARVRGVARVTLSVPAAAHGRMFPSSLASPAAAHGAAETSDGIISYVPISDGLEFGARPSSAAERARCRRASAESLSAVVRRLAGAGQPVTCVVSTISLPAIDVALEHGIPFAFYWIQTAAAFAAYYHHYFHGYEQLVAAHVADPEDEVSLPGLGPLPIRDLPSFLADSTGSEHSKASVEWLRAIFEHIGREKPIVLVNTLDMLEMTVLQAVRQHLDVFAVGPMVPHLQQIDVTHVTEDRVHLYKQDEKSYIEWLDAQPVRSVVYMSYGSMLTYNKQQVEEILQGFHACGRPYLWVVRKDGREDEVERCLAQNRCLEHGMIVDWCDQIEVLSHPSVGCFVTHCGWNSTLEAMTLGVPMVTVPTWSDQALNAHLVDEWGIGVRTRRNDEGLLLGTELAKCIEIVMGNDDKARNMHEKVNDLKGNVQSHIL
>rna-gnl|WGS:RWGY|EJB05_53602.mrna
MAPPHFLVVTFIGQGHLNPSRALAARIARATGARVTLSAAVSMHRRMFPSLAAADEEVDDGGPVSYAPYSDGYDDGFRFLGGDQWDYVHRSSRVGRETLAAVLDRLAARGRPVTCVVYTILLWWAGELARERGVPGVLFWAQPATVLALYHHYFHDEYYRRLVAEHAGDPGFAVAVPGLLPMAIGELPVLLTNSTGDTMLSAALYRIRKTMEQVDTDRRSSNGGGNAMVLVNTVEALEAGALTCIPGLDVLPIGPVFDASHGAQSAETYMEWLDTKPARSVVYVSFGSIADMSKRQKEEMRRGLASTGRPYLCVVRDNLDDDDDCGEEGQGVVVEWCDQARVLSHAAVGCFVTHCGWNSTLEAVASGVPMVAVPQCFEQHANARILVADWGVGVRAAVAADGVLEAEELSRCLEMVMGHNESAIAVRRSSEAWKAKVTEATGDGGSSDRNFKTFLNKTVKRP
MSPRPHFLVLTFPFQGHIAPALRLARRLLAAAPDALVTFSTTDIAHRRMFPAKDDAAAERGNDDDDGRLEFLPFSDGTEAGYVPSSDKGAFTAYMDSFHAEGARSVGELLDALAARGRPVSRVVYTLLLPWAADVARERGVPSALYWIQPAAVFAIYHHYFHGHAGVVDEHRHDPTFVVQLPGLPPQAIEDLPSFITQSTDPSDPLHSCFTTLRDLFDALDRETPKATVLVNTCEELEVGALAAVGAYDVLPVGPVLPSGDEVGLFKRDDAKYMEWLDSKPANSVVYVSFGSLAAMEREQLDELLLGLEESGRPYLCVVRKDIKASIFKQGRTEVIFGFTMRNNRV
>rna-gnl|WGS:RWGY|EJB05_41425.mrna
MESPPPPPPQHFLFVTNPMQGHINPARRLAARVLASTPGARVTFSTAVSGHRRMFPDLASPDEEAVDAAGVLHLPYSDSFDEGFNPAVHGLGSYKARAPAAGRETLSAVVARLAERGRPVTCLVYTFLVPWAADVARAHGVAAALYWIQPAAVFAVYYHYFHGHDAAVLAACANVSEPVQLPGLPPLGPDALPSVVFITSPEHRHYLLLDLLRDLFRSLDEHKPKVLVNTFDALEAEELRAVPQFELAAVGPVVPDDASSPSNSDLFHRHDATAYMEWLDTKPARSVVYVSFGSVLSMSARQEEEMRRGLEATGRPYLWVARKPADACAGNSDGGDGAQGMVVEWCDQVRVLSHPAVGCFVTHCGWNSTMESVTRGVPMVAVPQWTDQPTVAWLVEARMGAGVRARVDGEGVVERRELQRCVEAVMGDGDAAAAVREQAALWSKQAGEAVACGGTSERNLGAFASFRPAVDNA
>rna-gnl|WGS:RWGY|EJB05_41432.mrna
MQSHINPARRLAARVAAALPNARVTFSTAVSGHCQMFPHLTSPDGEVIQGLVSYIPYSDGFDCGFSPCGEAAGGRVCAYRARARAVGAETLAAVFARLAARGRPVTRVVYTALVGWVPGVARAHGVPAALYWVKPATVFAVYYHYFHGHGEVLHSIANNETDPDDASVHLPGLPPLKPDALPAFVSMASPGSRQYLTLDMLRDIFFALDEHMPTVLVDTFDALEYEALRALPQFDLVAVGPVVVEEPCRPCVALFQPNDAKAYMDWLDKKSARSVVFVSLGSVLSVSKLQDEELRRGLEATGRPYLLVTHKINSGGVAGDGGQGMVVEWCNQMGVLAHPAVGCFVTHCRWDSTLESITCGVPMVAVPRWADQPTVAALVETSVGVGVRAQVDGEGVLTRGELQRCVEMVMGSTHSAAAVRARAERWGQSAKEAAAAGGTSERSLRAFATGL
>rna-gnl|WGS:RWGY|EJB05_41411.mrna
MDWLDKKPARSVVFVSLGSVLSVSKRLDEQMRRGLEATGRPYLLVARKSSGGGVAGTGGQGMVVEWCNQMRVLAHPAVGCFVS
>rna-gnl|WGS:RWGY|EJB05_44821.mrna
MASPADDPCVKAQQHFLFVTGPLHGHINPVQRLAARVLAANPGARVTFSTAVSGHRAPAHIPVPRAAQRGDGGVLHAPYSDGYDDGFDPSAHDARLYRARTRDADRASLAAVVARLRPTVTRVAYTFLVAWALDVARDAGVPAALYWIQPATVFAVYHRVLHGGGHGDALAAACAGGGSVELPGLPALTADALPSIVSAASPEHPMHGAFQSFRDLFVDLDENRPRVLVNTFEALEPEALRAVPELEVVAVGPAVPDEASLSPRTTDDTTEDYMAWLDTKAARSVVYVSFGSFGAAGRGDEARPGCHRPAVPMGATKQDDEKEQEGMVVAWCEQVRVLSHPAVGCFVTHCGWNSALESMACGVPIVALPHWTDQPTVAWLLAERAGVGVRACAVAGDGVVESGELQRCVEAVMGDGQCAADIFSMNLEFRI
>rna-gnl|WGS:RWGY|EJB05_50925.mrna
MADADNHHSEYGGRGDSHFLVVANGMQGHLNPARALARRLARVRGVARVTLSVPAAAHGRMFPSSLASPAAAHGAAETSDGIISYVPISDGLEFGARPSSAAERARCRRASAESLSAVVRRLAGAGQPVTCVVSTISLPAIDVALEHGIPFAFYWIQTAAAFAAYYHHYFHGYEQLVAAHVADPEDEVSLPGLGPLPIRDLPSFLADSTGSEHSKASVEWLRAIFEHIGREKPIVLVNTLDMLEMTVLQAVRQHLDVFAVGPMVPHLQQIDVTHVTEDRVHLYKQDEKSYIEWLDAQPVRSVVYMSYGSMLTYNKQQVEEILQGFHACGRPYLWVVRKDGREDEVERCLAQNRCLEHGMIVDWCDQIEVLSHPSVGCFVTHCGWNSTLEAMTLGVPMVTVPTWSDQALNAHLVDEWGIGVRTRRNDEGLLLGTELAKCIEIVMGNDDKARNMHEKVNDLKGNVQSHIL
>rna-gnl|WGS:RWGY|EJB05_53602.mrna
MAPPHFLVVTFIGQGHLNPSRALAARIARATGARVTLSAAVSMHRRMFPSLAAADEEVDDGGPVSYAPYSDGYDDGFRFLGGDQWDYVHRSSRVGRETLAAVLDRLAARGRPVTCVVYTILLWWAGELARERGVPGVLFWAQPATVLALYHHYFHDEYYRRLVAEHAGDPGFAVAVPGLLPMAIGELPVLLTNSTGDTMLSAALYRIRKTMEQVDTDRRSSNGGGNAMVLVNTVEALEAGALTCIPGLDVLPIGPVFDASHGAQSAETYMEWLDTKPARSVVYVSFGSIADMSKRQKEEMRRGLASTGRPYLCVVRDNLDDDDDCGEEGQGVVVEWCDQARVLSHAAVGCFVTHCGWNSTLEAVASGVPMVAVPQCFEQHANARILVADWGVGVRAAVAADGVLEAEELSRCLEMVMGHNESAIAVRRSSEAWKAKVTEATGDGGSSDRNFKTFLNKTVKRP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University