Enzyme
Isolated from the plants Perilla frutescens var. crispa, Verbena hybrida [1], Dahlia variabilis [2] and Gentiana triflora (clustered gentian) [3]. It will also act on anthocyanidin 3-O-(6-O-malonylglucoside) [2] and is much less active with hydroxycinnamoylglucose derivatives [3]. There is no activity in the absence of the 3-O-glucoside group.
PF3R4
Enzyme
Anthocyanidin 3-O-glucoside 5-O-glucosyltransferase 1
Enzyme name
EC:2.4.1.298
Entry
Transferases;Glycosyltransferases;Hexosyltransferases
Class
UDP-alpha-D-glucose:anthocyanidin-3-O-beta-D-glucoside 5-O-glucosyltransferase
Sysname
UDP-alpha-D-glucose + an anthocyanidin 3-O-beta-D-glucoside = UDP + an anthocyanidin 3,5-di-O-beta-D-glucoside [RN:R10262]
Reaction
UDP [CPD:C00015];anthocyanidin 3,5-di-O-beta-D-glucoside [CPD:C06361]
Product
Species information
Eragrostis curvula
Species name
Weeping love grass (Eragrostis curvula) is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Eragrostis_curvula.PF3R4.fa
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>rna-gnl|WGS:RWGY|EJB05_41433.mrna
MSPRPHFLVLTFPFQGHIAPALRLARRLLAAAPDALVTFSTTDIAHRRMFPAKDDAAAERGNDDDDGRLEFLPFSDGTEAGYVPSSDKGAFTAYMDSFHAEGARSVGELLDALAARGRPVSRVVYTLLLPWAADVARERGVPSALYWIQPAAVFAIYHHYFHGHAGVVDEHRHDPTFVVQLPGLPPQAIEDLPSFITQSTDPSDPLHSCFTTLRDLFDALDRETPKATVLVNTCEELEVGALAAVGAYDVLPVGPVLPSGDEVGLFKRDDAKYMEWLDSKPANSVVYVSFGSLAAMEREQLDELLLGLEESGRPYLCVVRKDIKASIFKQGRTEVIFGFTMRNNRV
>rna-gnl|WGS:RWGY|EJB05_44811.mrna
MTLRATYAGVPAALYWIQPAATVFAAALYSIGHLAGAPIARGVLARRSATCLRTSTSTGPRCSVNTFDALEPEALRGLQQLDVVAVGLAVLEDVVSRHSRDDNATGAESTLLHGVAGHQGLAVRGLRVVRELRPP
>rna-gnl|WGS:RWGY|EJB05_41431.mrna
MSLRRPHFLVLTFPFQSHIAPALRLARRLLEASPDALVTFSTTEVAHGRMFPAKSTEEGAVERDTTDADGGDGRLEFVPFSDGTEAGRVRSSDAGAFNAYMESFHAEGARSVGELLDAFAARGRPVSRVVYTLLLPWAADVARERGIPSALYWIQPVAVFAIYHHYFHGLSSIVADHLHDPSFVVEIPGLAPQTIGDLPSFLTQSTEPSDPLHSGFTTKRDLPYLFVIREDNKAVLNEAEAGMGEGLKNGMIVQWCNQVRVLSHAAVGCFVTHCGWNSVLESMACGIPMVGVPRLSDQRMNVQLIEHEWHVGIRAEVDNSGILRVMELRRCIEEVMGKGEAATEVRRMAGQWKQVLEKSMGKEGSSDRNLVAFVSDATSG
>rna-gnl|WGS:RWGY|EJB05_44810.mrna
MKRGLEAAAAVHMGGAPARRSNTTRRWRSRSRVGLWWRGASRCACCRTRRWGASSRTAAGTRRSRAWPAGCPSLRCRSGRTSPRWRGCSRSARASACARAAGDGVVEATELQRCVEAVMGDDQHAADIRARVERYVEGARQTGGGHRRELGNESQGVRPRAVTHFMDLDRRNQEDDAATRKRRDNFIVVDKNTSLILTISNTIELGMQLFLTEGEAALTWRPGI
>rna-gnl|WGS:RWGY|EJB05_44821.mrna
MASPADDPCVKAQQHFLFVTGPLHGHINPVQRLAARVLAANPGARVTFSTAVSGHRAPAHIPVPRAAQRGDGGVLHAPYSDGYDDGFDPSAHDARLYRARTRDADRASLAAVVARLRPTVTRVAYTFLVAWALDVARDAGVPAALYWIQPATVFAVYHRVLHGGGHGDALAAACAGGGSVELPGLPALTADALPSIVSAASPEHPMHGAFQSFRDLFVDLDENRPRVLVNTFEALEPEALRAVPELEVVAVGPAVPDEASLSPRTTDDTTEDYMAWLDTKAARSVVYVSFGSFGAAGRGDEARPGCHRPAVPMGATKQDDEKEQEGMVVAWCEQVRVLSHPAVGCFVTHCGWNSALESMACGVPIVALPHWTDQPTVAWLLAERAGVGVRACAVAGDGVVESGELQRCVEAVMGDGQCAADIFSMNLEFRI
>rna-gnl|WGS:RWGY|EJB05_41414.mrna
MASPQQQQHFLFVTDPMQSHVNPARRLAARVAAALPNARVTFSTAVSGHRQMFPHLTSPDGEVIQGLVSYIPYSDGFDGGFSPGEDARGRGAPATVFAVYYYYFHGHGEALHYVANSDTDPEDDASVHLPGLPPLKPDALPCFASMASPGSRHYLTLDMLRDIFFAPDEHMPTVLVDTFDALEYEALRALPQFDLVAV
>rna-gnl|WGS:RWGY|EJB05_41421.mrna
LRPADRIATDHPLTGSCTSSSSRPTESPPQQQQHFLFVTNPMQGHINPVRRLAARVLGSTPGARVTFSTAVSGHRRMFPDLTSPDDEAVDAAGVLHVPYSDEHEPKGLVVEWCDQVRVLSHPAVGCFVTHCGWNSTLESVTRGVPMVAVPQWTDQPTVAWLVEARMGAGVRARVDGEGVVERRELQRCVETVMGDGNAAAVIREQAALWKEWAGEAVAFGGTSEMNLCAFASLPSSA
>rna-gnl|WGS:RWGY|EJB05_57611.mrna
MAAAATATVARPHALLIPYPCSGHINPTLHFAFAYPFPFVIDAATINVKAHSEHELVVLTVLPMTMASTEGD
>rna-gnl|WGS:RWGY|EJB05_50936.mrna
MADEGRRHFLIVMYGIQSHINPVRVLAHRLTRLGVDGSIRATLSLPIATHRRLFSSSDDVADGVISYIPYSDGLDDGSLLKDADERARTRRAGFESVSSIIARRLARVRGVARLTLSVPAAAHGRMFPASLASPAAGDDEDEAEASDGVISYVPFSDGLEFGARPSSAAERARCRRESAESLSAVVRRLAAAGKPVTCVVSTISLPAIDVALEHGIPFAFYWIQTAAAFAAYYHYFRGYEQLVTAHVADPAYEVSFLGLRPLPISDLPSFLADSTGSEQSKASIELLRAIFEHIGREKPKVLVNSLDMLETDLLQAVRQHVEVFAVGPMVPHLQQMDVTEDRVHLYKQEEKGYMEWLNGQPGKSVVYMSYGSMITYEKKKTSGGMASMLVGGHTCGWCGRTMAVKMRWKGCFVTHCGWNSTLEAMTLGVPMVTVPNWSDQALNAHLVDEWGIRVRAKRDDEGLLVGTELAKCIEIVMSNDDKARNIHEKVNNLKDNTNGAIEDGLHWEGLDDGSLPKDADERARTRRAGFESVSSIIASLAARGQPVTCIMCTMVLPSVLDAAKEHGIPLAVYWIQPTTTTSTATASSSLPTPLTWTTRSACPG
>rna-gnl|WGS:RWGY|EJB05_31156.mrna
MPAMEEERTPAAAEPHFLIVTYPAQGHINPARHLARRLLRATGARVTVSTAVSAFRKMFPGAAESDEEEKGHRDGAGIWHVPYSDGYDGGFDRAVHDHTHYMTHIKVEGSRTLACVLDRLRARPARDARRPATVLAAYLHFFRGTDGVDKTIVEAAKSGDPWAEVTVPGLPPLRVRDLPSFITMTSEDDPYAFVVTAFRELVLALDRDDTPTVLANTFDAMEPEAVASLRDHGVDVVPVGPVAVLSHPAVGCFVTHCGWNSTLEAVACGVPVVCAPQWTDQGTNAWLVERLGTGVRAAVSDKLEGGGGVIEADELRRCIDFATSDAVRAKAAVWSEKARAAAAEGGSSEKNLRAFVAQQLPAGGTN
MSPRPHFLVLTFPFQGHIAPALRLARRLLAAAPDALVTFSTTDIAHRRMFPAKDDAAAERGNDDDDGRLEFLPFSDGTEAGYVPSSDKGAFTAYMDSFHAEGARSVGELLDALAARGRPVSRVVYTLLLPWAADVARERGVPSALYWIQPAAVFAIYHHYFHGHAGVVDEHRHDPTFVVQLPGLPPQAIEDLPSFITQSTDPSDPLHSCFTTLRDLFDALDRETPKATVLVNTCEELEVGALAAVGAYDVLPVGPVLPSGDEVGLFKRDDAKYMEWLDSKPANSVVYVSFGSLAAMEREQLDELLLGLEESGRPYLCVVRKDIKASIFKQGRTEVIFGFTMRNNRV
>rna-gnl|WGS:RWGY|EJB05_44811.mrna
MTLRATYAGVPAALYWIQPAATVFAAALYSIGHLAGAPIARGVLARRSATCLRTSTSTGPRCSVNTFDALEPEALRGLQQLDVVAVGLAVLEDVVSRHSRDDNATGAESTLLHGVAGHQGLAVRGLRVVRELRPP
>rna-gnl|WGS:RWGY|EJB05_41431.mrna
MSLRRPHFLVLTFPFQSHIAPALRLARRLLEASPDALVTFSTTEVAHGRMFPAKSTEEGAVERDTTDADGGDGRLEFVPFSDGTEAGRVRSSDAGAFNAYMESFHAEGARSVGELLDAFAARGRPVSRVVYTLLLPWAADVARERGIPSALYWIQPVAVFAIYHHYFHGLSSIVADHLHDPSFVVEIPGLAPQTIGDLPSFLTQSTEPSDPLHSGFTTKRDLPYLFVIREDNKAVLNEAEAGMGEGLKNGMIVQWCNQVRVLSHAAVGCFVTHCGWNSVLESMACGIPMVGVPRLSDQRMNVQLIEHEWHVGIRAEVDNSGILRVMELRRCIEEVMGKGEAATEVRRMAGQWKQVLEKSMGKEGSSDRNLVAFVSDATSG
>rna-gnl|WGS:RWGY|EJB05_44810.mrna
MKRGLEAAAAVHMGGAPARRSNTTRRWRSRSRVGLWWRGASRCACCRTRRWGASSRTAAGTRRSRAWPAGCPSLRCRSGRTSPRWRGCSRSARASACARAAGDGVVEATELQRCVEAVMGDDQHAADIRARVERYVEGARQTGGGHRRELGNESQGVRPRAVTHFMDLDRRNQEDDAATRKRRDNFIVVDKNTSLILTISNTIELGMQLFLTEGEAALTWRPGI
>rna-gnl|WGS:RWGY|EJB05_44821.mrna
MASPADDPCVKAQQHFLFVTGPLHGHINPVQRLAARVLAANPGARVTFSTAVSGHRAPAHIPVPRAAQRGDGGVLHAPYSDGYDDGFDPSAHDARLYRARTRDADRASLAAVVARLRPTVTRVAYTFLVAWALDVARDAGVPAALYWIQPATVFAVYHRVLHGGGHGDALAAACAGGGSVELPGLPALTADALPSIVSAASPEHPMHGAFQSFRDLFVDLDENRPRVLVNTFEALEPEALRAVPELEVVAVGPAVPDEASLSPRTTDDTTEDYMAWLDTKAARSVVYVSFGSFGAAGRGDEARPGCHRPAVPMGATKQDDEKEQEGMVVAWCEQVRVLSHPAVGCFVTHCGWNSALESMACGVPIVALPHWTDQPTVAWLLAERAGVGVRACAVAGDGVVESGELQRCVEAVMGDGQCAADIFSMNLEFRI
>rna-gnl|WGS:RWGY|EJB05_41414.mrna
MASPQQQQHFLFVTDPMQSHVNPARRLAARVAAALPNARVTFSTAVSGHRQMFPHLTSPDGEVIQGLVSYIPYSDGFDGGFSPGEDARGRGAPATVFAVYYYYFHGHGEALHYVANSDTDPEDDASVHLPGLPPLKPDALPCFASMASPGSRHYLTLDMLRDIFFAPDEHMPTVLVDTFDALEYEALRALPQFDLVAV
>rna-gnl|WGS:RWGY|EJB05_41421.mrna
LRPADRIATDHPLTGSCTSSSSRPTESPPQQQQHFLFVTNPMQGHINPVRRLAARVLGSTPGARVTFSTAVSGHRRMFPDLTSPDDEAVDAAGVLHVPYSDEHEPKGLVVEWCDQVRVLSHPAVGCFVTHCGWNSTLESVTRGVPMVAVPQWTDQPTVAWLVEARMGAGVRARVDGEGVVERRELQRCVETVMGDGNAAAVIREQAALWKEWAGEAVAFGGTSEMNLCAFASLPSSA
>rna-gnl|WGS:RWGY|EJB05_57611.mrna
MAAAATATVARPHALLIPYPCSGHINPTLHFAFAYPFPFVIDAATINVKAHSEHELVVLTVLPMTMASTEGD
>rna-gnl|WGS:RWGY|EJB05_50936.mrna
MADEGRRHFLIVMYGIQSHINPVRVLAHRLTRLGVDGSIRATLSLPIATHRRLFSSSDDVADGVISYIPYSDGLDDGSLLKDADERARTRRAGFESVSSIIARRLARVRGVARLTLSVPAAAHGRMFPASLASPAAGDDEDEAEASDGVISYVPFSDGLEFGARPSSAAERARCRRESAESLSAVVRRLAAAGKPVTCVVSTISLPAIDVALEHGIPFAFYWIQTAAAFAAYYHYFRGYEQLVTAHVADPAYEVSFLGLRPLPISDLPSFLADSTGSEQSKASIELLRAIFEHIGREKPKVLVNSLDMLETDLLQAVRQHVEVFAVGPMVPHLQQMDVTEDRVHLYKQEEKGYMEWLNGQPGKSVVYMSYGSMITYEKKKTSGGMASMLVGGHTCGWCGRTMAVKMRWKGCFVTHCGWNSTLEAMTLGVPMVTVPNWSDQALNAHLVDEWGIRVRAKRDDEGLLVGTELAKCIEIVMSNDDKARNIHEKVNNLKDNTNGAIEDGLHWEGLDDGSLPKDADERARTRRAGFESVSSIIASLAARGQPVTCIMCTMVLPSVLDAAKEHGIPLAVYWIQPTTTTSTATASSSLPTPLTWTTRSACPG
>rna-gnl|WGS:RWGY|EJB05_31156.mrna
MPAMEEERTPAAAEPHFLIVTYPAQGHINPARHLARRLLRATGARVTVSTAVSAFRKMFPGAAESDEEEKGHRDGAGIWHVPYSDGYDGGFDRAVHDHTHYMTHIKVEGSRTLACVLDRLRARPARDARRPATVLAAYLHFFRGTDGVDKTIVEAAKSGDPWAEVTVPGLPPLRVRDLPSFITMTSEDDPYAFVVTAFRELVLALDRDDTPTVLANTFDAMEPEAVASLRDHGVDVVPVGPVAVLSHPAVGCFVTHCGWNSTLEAVACGVPVVCAPQWTDQGTNAWLVERLGTGVRAAVSDKLEGGGGVIEADELRRCIDFATSDAVRAKAAVWSEKARAAAAEGGSSEKNLRAFVAQQLPAGGTN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University