Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Eragrostis curvula
Species name
Weeping love grass (Eragrostis curvula) is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Eragrostis_curvula.FLS.fa
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>rna-gnl|WGS:RWGY|EJB05_22415.mrna
MAEVQQSVQALSVSGAVPPEFVRLEHDQPGGATFQDTGLEEAPVIDMSKPDCGSRMVEAAMEWGSFQVVNHGVPAAAVAELQSVGRAFFALPQEEKDRYAADPASGRIEGYGVGTRAPRRNLAGKKMWADYFYHYVAPQAIVNHDIWPNNPAGYREANEEYCRHMQRLTREMFEHLSTGLGLEKGAMSEAFGGDELVLLQKINSYPPCPQPDVVLGVGSHTDMCTLTILLPNDVSGLQVFKDGRWHDVKYIPGALVVLIADQIEILSNGRYKAGMHRAMVNKEKTRMSWPVFVEPPRELVVGPHQLLATDDNPAKYKAKKYKDYQYCKINLLPQ
>rna-gnl|WGS:RWGY|EJB05_20188.mrna
MEYTSRHTDGKRINTRSARRPCFLPGRIEGYGTKLQRDIDGKKTWNDFFFHVVAPPAMLDHGVWPDSPTGYRAANEEYCGHMMRLTRALFEHLSLGLGLEKGAMAEAFGGDELVFLQKINFYPPCPQPELTLGVAPHTDMSTLTVLVPNEVQGLQVFRDGHWYDVKHVPDALIVHIGDQIEASMNELEPN
>rna-gnl|WGS:RWGY|EJB05_20200.mrna
MGEPHQSVQALATSLRALPPEFVRPEQEQPGATTFRGSAAPEAPVIDMSEPGCGARMADAAREWGLFEVVNHGVPAAAVAELQRVGRAFFSLPQALQKQKFQTGTKAHSRRERQPSVTARLRCLTLSDGEIHRLKQ
>rna-gnl|WGS:RWGY|EJB05_22278.mrna
MAEVQQSVQALSVSGAVPPEFVRLEHDQPGGATFQDTGLEEAPVIDMSKPDCGSRMVEAAMEWGSFQVVNHGVPAAAVAELQSVGRAFFALPQEEKDRYAADPASGRIEGYGVGTRAPRRNLAGKKMWADYFYHYVAPLAIVNHDIWPNNPAGYREANEEYCRHMQRLTREMFEHLSTGLGLEKGAMSEAFGGDELVLLQKINSYPPCPQPDVVLGVGSHTDMCTLTILLPNDVSGLQVFKDGRWHDVKYIPGALVVLIADQIEILSNGRYKAGMHRAMVNKEKTRMSWPVFVEPPRELVVGPHQLLATDDNPAKYKAKKYKDYQYCKINLLPQ
>rna-gnl|WGS:RWGY|EJB05_20185.mrna
MGSAAALRPPPVLRHPPPLLRCWDGEPDAEVLAEEARRRMRLAIVEALRRRLGDGEVIPYADLLCACEDSGAARMRAEAAALAGALDEAGVVLLFRDKVCLQSDKEMQRPSLWDWSQLGADTGGASSARGAGRGSDAFYRCHARDGRRGGRDFFRCRHRITGERGVECPLCHSLAAIFSNGKYKAVLHRTTVNKDKTRMSWPVFVEPPRELVVGPQSQLVTDENPAKYKAKKYKDYQYCKINKLPM
>rna-gnl|WGS:RWGY|EJB05_20182.mrna
MVRSPCCEKAHTNKGAWTKEEDERLVAYIRAHGEGCWRSLPQAAGLLRCGKSCRRRWINYLRPDLKHGNFTPDEDSLIVKLHAALGNKWSLIAARLPGRAGNEIKNYWNKHLTATITTTQVVKAAKAAPRCPDLNLDLCISPPSPPDLNLDLCISPPAPLPFKAAPRCPDLNLELCIKEEEEEEDVKPVVKLQALATSLRALPLEFVRPEQEQPGATTFRGPAAPEAPVIDMSEPGCGARMADAAREWGLFQVVNHGVPAAAVAELQRVGRAFFALPQAEKERYAMDPASGRIEGYGTKLQRDLDGKKTWNDFFFHVVAPPALVDHGVWPASPAGYRAANEEYCGHMMRLTRALFGHLSLGLGLEEGAMAEAFGGDELVFLQKINLYPPCPQPELTLGVAPHTDMSTLTVLVPNEVQGLQLFRDGHWYDVKHIFSNGKYKAVLHRTTVNKDKTRMSWPVFVEPPRELVVGPHPQLVTDESPAKYKAKKYKDYQYCKINKLPIGFEVPSNGCELPLSCGALGLCTPKGCTCPPLFAASQDGGCAPSDGSTALSVGSCNGGGSAGGNSSPASYASLGNGHELGSFVSTNVTGGSDKLGYIKVLSSKSNGVAYHANKFAAPDMAGRNISSCQALCTSNCSCLGYFYVESSLSCYLVQHELGSFISTNVTGGSDKLGYIKVLSSQSQGASKGSSSNSTLIAILLPTIVAFVLIVVVSAVVITSWRKEEGRRSGRDLQLRRQRSPSDSTHLVREIKDDGGDDIVIPGLPTRFTHEEIEDMTNSFRIKIGAGGFGAVYKGVLPDGSLVAVKKIEGVGMQGKREFCTEIAVIGNIHHVNLVRLRGFCTEGQRRLLVYEYMNRGSLDRSLFRPTGPQLLDWKERMDVAVGAARGLAYLHFGCDQRIIHCDVKPENILLADGGLVKIADFGLAKFLTPEQSGLFTTMRGTRGYLAPEWLSNAPITDRTDVYSFGMVLLELVRGRKNRSEHVSDNGGEASSSSNGTTGSSSKSDYFPLAALEAHEAGQYAALADPRLQGKVNAKEVERVVKVALCCLHEDPHLRPSMAVVVGMLEGTMDLKEPCVQSLGFLRLYGRGFAGSAAGGSSDPNHMGSAGDRSGTTTLTTMSGWPSYMSSSQLSGPR
>rna-gnl|WGS:RWGY|EJB05_20199.mrna
MDAASGRIEGYGTKLQRDLDGKKTWNDFFFHVVAPPAMVDHGVWPASPAGYRAANEEYCGHMMRWTRALFEHLSLGLGLGLDKGAMAEAFGGDELVFLQKINFYPPCPQLELTLGVAPHTDMSTLTVLVPNEVQGLQVFRDGHWYDAKYVPDALVIFSNGKYKAVLHRTTVNKDKTRMSWPVFVEPPRELVVGPHPQLVTDESPAKYKAKKYKDYQYCKINKLPM
>rna-gnl|WGS:RWGY|EJB05_20184.mrna
MGKPHQSVQALATSLRALPPEFVRPEQEQPGATTFRGPAAPEAPVIDMSEPGCGARMAEAARDWGLFQVVNHGVPAAAVAELQRVGRAFFALPQAEKEQYAMDPASGRIEGYGTKLQRDLDGKKTWNDFFFHVVAPPAMVDHGVWPDSPAGYRAANEEYCGHMMRLTRALFEHLSLGLGLEKGAMAEAFGGDELVFLQKINFYPPCPQPELTLGVAPHTDMSTLTVLVPNEVQGLQVFRDGHWYDAKYIFSNGKYKAVLHRTTVNKDKTRMSWPVFVEPPRELVVGPHPQLVTDESPAKYKAKKYNDYQYCKINKLPM
>rna-gnl|WGS:RWGY|EJB05_49236.mrna
MAEVHQSVQAMSLAGAILPEFVRSEQEQPGTTNSFCATGTVPEVPPVLDMAQPGCGARMADAATEWGFFQVVNHGVPAATIAELQRVGRTFFALPQEEKERYAATDPTSGRSEGYGVGTKASRNLEEGNKLWADFFYHYVAPAAMVNHHIWPKNPASYRYGHIPNVRNCFHESTCVLSSPAGDMIDSREVTEEYAGNMLQLTREMLEHLSVGLGVEKGALMESLAGSEDELVLLQKINSYPPCPQPDVILGIGPHTDMGTLTVLLPDEVPGLQICKDGRWHDVEYVPEALVVHIGDQIEASSTRIVSNGRYKPGMHRAMVNKEKTRMSWPLFVEPPPELVVGPHPQLLAAADNGLTKYKTKKYKDYQYCKLNKLPQ
>rna-gnl|WGS:RWGY|EJB05_50304.mrna
MAEVHQSVQAMSLAGAIPPEFVRSEQEQPGTTNSFCATGTVPEVPPVLDMAQPGCGARMADAATEWGFFQVVNHGVPAATIAELQRVGRAFFALPQEEKERYAATDPTSGRSEGYGVGTKASRNLEEGNKLWADFFYHYVAPAAMVNHHIWPKNPASYRYGHIPNVRNCFHESTCVLSSPAGDMIDSREVTEEYAGNMLRLTREMLEHLSVGLGVEKGALMESLAGGEDELVLLQKINSYPPCPQPDVILGIGPHTDMGTLTVLLPDEVPGLQICKDGRWHDVEYVPEELVIVSNGRYKPGMHRAMVNKEKTRMSWPLFVEPPPELVVGPHPQLLAAADNGLTKYKTKKYKDYQYCKLNKLPQ
MAEVQQSVQALSVSGAVPPEFVRLEHDQPGGATFQDTGLEEAPVIDMSKPDCGSRMVEAAMEWGSFQVVNHGVPAAAVAELQSVGRAFFALPQEEKDRYAADPASGRIEGYGVGTRAPRRNLAGKKMWADYFYHYVAPQAIVNHDIWPNNPAGYREANEEYCRHMQRLTREMFEHLSTGLGLEKGAMSEAFGGDELVLLQKINSYPPCPQPDVVLGVGSHTDMCTLTILLPNDVSGLQVFKDGRWHDVKYIPGALVVLIADQIEILSNGRYKAGMHRAMVNKEKTRMSWPVFVEPPRELVVGPHQLLATDDNPAKYKAKKYKDYQYCKINLLPQ
>rna-gnl|WGS:RWGY|EJB05_20188.mrna
MEYTSRHTDGKRINTRSARRPCFLPGRIEGYGTKLQRDIDGKKTWNDFFFHVVAPPAMLDHGVWPDSPTGYRAANEEYCGHMMRLTRALFEHLSLGLGLEKGAMAEAFGGDELVFLQKINFYPPCPQPELTLGVAPHTDMSTLTVLVPNEVQGLQVFRDGHWYDVKHVPDALIVHIGDQIEASMNELEPN
>rna-gnl|WGS:RWGY|EJB05_20200.mrna
MGEPHQSVQALATSLRALPPEFVRPEQEQPGATTFRGSAAPEAPVIDMSEPGCGARMADAAREWGLFEVVNHGVPAAAVAELQRVGRAFFSLPQALQKQKFQTGTKAHSRRERQPSVTARLRCLTLSDGEIHRLKQ
>rna-gnl|WGS:RWGY|EJB05_22278.mrna
MAEVQQSVQALSVSGAVPPEFVRLEHDQPGGATFQDTGLEEAPVIDMSKPDCGSRMVEAAMEWGSFQVVNHGVPAAAVAELQSVGRAFFALPQEEKDRYAADPASGRIEGYGVGTRAPRRNLAGKKMWADYFYHYVAPLAIVNHDIWPNNPAGYREANEEYCRHMQRLTREMFEHLSTGLGLEKGAMSEAFGGDELVLLQKINSYPPCPQPDVVLGVGSHTDMCTLTILLPNDVSGLQVFKDGRWHDVKYIPGALVVLIADQIEILSNGRYKAGMHRAMVNKEKTRMSWPVFVEPPRELVVGPHQLLATDDNPAKYKAKKYKDYQYCKINLLPQ
>rna-gnl|WGS:RWGY|EJB05_20185.mrna
MGSAAALRPPPVLRHPPPLLRCWDGEPDAEVLAEEARRRMRLAIVEALRRRLGDGEVIPYADLLCACEDSGAARMRAEAAALAGALDEAGVVLLFRDKVCLQSDKEMQRPSLWDWSQLGADTGGASSARGAGRGSDAFYRCHARDGRRGGRDFFRCRHRITGERGVECPLCHSLAAIFSNGKYKAVLHRTTVNKDKTRMSWPVFVEPPRELVVGPQSQLVTDENPAKYKAKKYKDYQYCKINKLPM
>rna-gnl|WGS:RWGY|EJB05_20182.mrna
MVRSPCCEKAHTNKGAWTKEEDERLVAYIRAHGEGCWRSLPQAAGLLRCGKSCRRRWINYLRPDLKHGNFTPDEDSLIVKLHAALGNKWSLIAARLPGRAGNEIKNYWNKHLTATITTTQVVKAAKAAPRCPDLNLDLCISPPSPPDLNLDLCISPPAPLPFKAAPRCPDLNLELCIKEEEEEEDVKPVVKLQALATSLRALPLEFVRPEQEQPGATTFRGPAAPEAPVIDMSEPGCGARMADAAREWGLFQVVNHGVPAAAVAELQRVGRAFFALPQAEKERYAMDPASGRIEGYGTKLQRDLDGKKTWNDFFFHVVAPPALVDHGVWPASPAGYRAANEEYCGHMMRLTRALFGHLSLGLGLEEGAMAEAFGGDELVFLQKINLYPPCPQPELTLGVAPHTDMSTLTVLVPNEVQGLQLFRDGHWYDVKHIFSNGKYKAVLHRTTVNKDKTRMSWPVFVEPPRELVVGPHPQLVTDESPAKYKAKKYKDYQYCKINKLPIGFEVPSNGCELPLSCGALGLCTPKGCTCPPLFAASQDGGCAPSDGSTALSVGSCNGGGSAGGNSSPASYASLGNGHELGSFVSTNVTGGSDKLGYIKVLSSKSNGVAYHANKFAAPDMAGRNISSCQALCTSNCSCLGYFYVESSLSCYLVQHELGSFISTNVTGGSDKLGYIKVLSSQSQGASKGSSSNSTLIAILLPTIVAFVLIVVVSAVVITSWRKEEGRRSGRDLQLRRQRSPSDSTHLVREIKDDGGDDIVIPGLPTRFTHEEIEDMTNSFRIKIGAGGFGAVYKGVLPDGSLVAVKKIEGVGMQGKREFCTEIAVIGNIHHVNLVRLRGFCTEGQRRLLVYEYMNRGSLDRSLFRPTGPQLLDWKERMDVAVGAARGLAYLHFGCDQRIIHCDVKPENILLADGGLVKIADFGLAKFLTPEQSGLFTTMRGTRGYLAPEWLSNAPITDRTDVYSFGMVLLELVRGRKNRSEHVSDNGGEASSSSNGTTGSSSKSDYFPLAALEAHEAGQYAALADPRLQGKVNAKEVERVVKVALCCLHEDPHLRPSMAVVVGMLEGTMDLKEPCVQSLGFLRLYGRGFAGSAAGGSSDPNHMGSAGDRSGTTTLTTMSGWPSYMSSSQLSGPR
>rna-gnl|WGS:RWGY|EJB05_20199.mrna
MDAASGRIEGYGTKLQRDLDGKKTWNDFFFHVVAPPAMVDHGVWPASPAGYRAANEEYCGHMMRWTRALFEHLSLGLGLGLDKGAMAEAFGGDELVFLQKINFYPPCPQLELTLGVAPHTDMSTLTVLVPNEVQGLQVFRDGHWYDAKYVPDALVIFSNGKYKAVLHRTTVNKDKTRMSWPVFVEPPRELVVGPHPQLVTDESPAKYKAKKYKDYQYCKINKLPM
>rna-gnl|WGS:RWGY|EJB05_20184.mrna
MGKPHQSVQALATSLRALPPEFVRPEQEQPGATTFRGPAAPEAPVIDMSEPGCGARMAEAARDWGLFQVVNHGVPAAAVAELQRVGRAFFALPQAEKEQYAMDPASGRIEGYGTKLQRDLDGKKTWNDFFFHVVAPPAMVDHGVWPDSPAGYRAANEEYCGHMMRLTRALFEHLSLGLGLEKGAMAEAFGGDELVFLQKINFYPPCPQPELTLGVAPHTDMSTLTVLVPNEVQGLQVFRDGHWYDAKYIFSNGKYKAVLHRTTVNKDKTRMSWPVFVEPPRELVVGPHPQLVTDESPAKYKAKKYNDYQYCKINKLPM
>rna-gnl|WGS:RWGY|EJB05_49236.mrna
MAEVHQSVQAMSLAGAILPEFVRSEQEQPGTTNSFCATGTVPEVPPVLDMAQPGCGARMADAATEWGFFQVVNHGVPAATIAELQRVGRTFFALPQEEKERYAATDPTSGRSEGYGVGTKASRNLEEGNKLWADFFYHYVAPAAMVNHHIWPKNPASYRYGHIPNVRNCFHESTCVLSSPAGDMIDSREVTEEYAGNMLQLTREMLEHLSVGLGVEKGALMESLAGSEDELVLLQKINSYPPCPQPDVILGIGPHTDMGTLTVLLPDEVPGLQICKDGRWHDVEYVPEALVVHIGDQIEASSTRIVSNGRYKPGMHRAMVNKEKTRMSWPLFVEPPPELVVGPHPQLLAAADNGLTKYKTKKYKDYQYCKLNKLPQ
>rna-gnl|WGS:RWGY|EJB05_50304.mrna
MAEVHQSVQAMSLAGAIPPEFVRSEQEQPGTTNSFCATGTVPEVPPVLDMAQPGCGARMADAATEWGFFQVVNHGVPAATIAELQRVGRAFFALPQEEKERYAATDPTSGRSEGYGVGTKASRNLEEGNKLWADFFYHYVAPAAMVNHHIWPKNPASYRYGHIPNVRNCFHESTCVLSSPAGDMIDSREVTEEYAGNMLRLTREMLEHLSVGLGVEKGALMESLAGGEDELVLLQKINSYPPCPQPDVILGIGPHTDMGTLTVLLPDEVPGLQICKDGRWHDVEYVPEELVIVSNGRYKPGMHRAMVNKEKTRMSWPLFVEPPPELVVGPHPQLLAAADNGLTKYKTKKYKDYQYCKLNKLPQ
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University