Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Draba nivalis
Species name
Draba nivalis is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Draba_nivalis.FLS.fa
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>maker-lg2-snap-gene-18.255-mRNA-1
MEIERVQDISSSSLLAESIPSEFIRSEKEQPAITTFRGPTPAIPVVDLSNPDEESVLRAVVKASEEWGIFQVVNHGIPTELIRRLQEVGRKFFELPSSEKETVAKPEDAKDIEGYGTKLQKEIDGKKAWVDHLFHRIWPKSRVNYRFWPENPPEYREVNEEYALHVKKLSETLLGILSEGLGLSSDALKVGLGGEKLEYMMKINYYPPCPRPDLALGVPAHTDLSGLTLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQLLRLSNGRYKNVLHRTTVDKDKTRMSWPVFLEPDREMIVGPLPELTGDDNPPKFKPLAFKDYSYRKLNKLPLD
>maker-lg2-snap-gene-60.122-mRNA-1
MEVQRNQHISPSSLLASDKEKSALEFQATSEDVPVIDLSNPNEDFLARTVVKVSEEWGIFKVVNHGIPTELMRRLKEVGTEFFELPETEKEVVARPAGSTNIEGYNTNAYNNKEGVKVWADHLFHTLWPPSRINYKFWPKNSPDYREVNEEYAKHIKKLSEKIMGWLSEGLGLHREALKEGLGGETVEYMMKVIFYPPCPDLDLLYGAPPHTDLNGITCLIANDVHGLQAFKDNNWIDVKYDESVIIIIIADQITRMSNGKYKSGQHRATMDTERTRISWPVFAEPHLDHVVGTLPELTTGDDEKAPKFKPYVYRDYKYLINPICFYQFTVLPCVGCVTEGIRACCIKRRWIRSEQAWPTRMTWPVLVNPNPGLVVGPLVELTGDDDPPKFESFYFHFFGRPQIALEENDVEHNICHYVVLKC
>maker-lg2-snap-gene-51.171-mRNA-1
MELPVVDLSRYLESSGPETDLLDSCREVSRILRETGALLVKDPRCTAQDNDRFIDMMETYFQMPDDFKRLQQRPNLHYQVGVTPEGVEVPRSLVDEEMQEKLNAMPEEYKPHTPKGPDHKWRYMWRAGPRPSNTRFKELNSEPVIPQGFPEWKEVMDSWGYKMISTVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYDEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPLGCLLIQTGKQIEWLTGGECIAGMHEVVVTSKTIDAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFAESSLASNYPAIPAGEYVEQELSVINLKGKKSRS
>maker-lg3-snap-gene-71.210-mRNA-1
MGLERDQETKQSSHIPVVDLSNPDQELVARAVVKASEEWGVFQLVNHGIPTELRQRLQNVGKQFFELPDAEKEAMAKPEYSREGYARRYEFDLEKKIGTVDQLFNIIWPPSSVDYKYWPKNLQDYRKVNEEYATHVRMLSEKIMEWLSEGLGLQREAIKEVIAGEYMLIVNYYPPCPHSDLIEGLDAHTDVSGLTLILPNEIPGLQVLKDDHWIELENIPSAIIVLIADQILRLSNGRYKSVLHKTTVDKERTRMSWPVLVRPTSDTVVGPFPELIGQDDPPKFKPMAYKDYIYRKVRNLSFVDLDST
>snap_masked-lg3-processed-gene-71.75-mRNA-1
MVDERDLDDMQSSHIPVVDLNNPDQELVAHAVVKASEEWGIFQVVNHGIPSELIQRLQNVGRQFFELPDVEKETVAKPADSREGYKRTNELDLKKKRGTIYKLLHNIWPPSSVNYTYWPKNPQDYREVNEEYTRQMKMLSEKIMEWLSEGLGLRREAFKDVMREYMMIVNYYPPCPHPDLIQGLEPHTDTSGFTLLLPNEVPGLQVFRDDHWIDFEYIPSAIIVVIGDQMLRLSNGRYKNVLHKTTVDKERIRMSWPVLVKPTYDTVIEPFAELTGQNDPPKFKPIAYKDYIYRKFSKLPMISTDK
>snap_masked-lg3-processed-gene-71.78-mRNA-1
MVLERDQDIQSSHIPVVDLSNPDQELVAHAVVKASEDWGIFQVVHHGIPTELIQRLQNVGKQFFELPDAEKEAVAKPVNSREGYVRRELDLENKIGTIYHLSHNIWPPSSVNYTYWPKNPQDYREVNEEYTRQMKMLSEKIMEWLSEGLGLQREAVKDVMGDYMMVVNYYPPCPHSDLIEGLETHTDISGFTLLQPNEVPGLQVFKDDHWIELEYVPSAIIVVIGDQLLRLSNGRYKNVLHKTTVDKERTRMSWPLLVRPTYDTVIRPFPELTSHDDPPKFKPISYKDYVYRKFSKLPIDVMCEFDLSKTLIILKVIRPFPELTGHDNPPKFN
>snap_masked-lg3-processed-gene-71.80-mRNA-1
MGWLSEGLGLHREALKEGLGGETVEYLMKVIFYPPCPKMDLLFGAPPHTDPNGITFLIADQVHGLQAFQDNKWIDVEYNDSVIVVIIADQITRMSNGRYKSGEHRATMDTVKTRISWPAFAEPNLDHVVGTFPELITGDDENAPKFKPYDYREFKFRKMNKLPID
>snap_masked-lg6-processed-gene-108.60-mRNA-1
MVACWPEPIVSVQALSQTGVLTVPSRYVKPLHQRPVFSSDQTEADVEIPVVDMSGVWGKQERLRHVRSACEEWGFFQVVNHGVSHGLMERMRGAWRDFFELPLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSVRNPSKWPAQPPKIRFLQSY
>maker-lg6-snap-gene-97.149-mRNA-1
MSVELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSGDMYQKLRRLSGGVFGLRDEEKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDRPRDEESS
MEIERVQDISSSSLLAESIPSEFIRSEKEQPAITTFRGPTPAIPVVDLSNPDEESVLRAVVKASEEWGIFQVVNHGIPTELIRRLQEVGRKFFELPSSEKETVAKPEDAKDIEGYGTKLQKEIDGKKAWVDHLFHRIWPKSRVNYRFWPENPPEYREVNEEYALHVKKLSETLLGILSEGLGLSSDALKVGLGGEKLEYMMKINYYPPCPRPDLALGVPAHTDLSGLTLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQLLRLSNGRYKNVLHRTTVDKDKTRMSWPVFLEPDREMIVGPLPELTGDDNPPKFKPLAFKDYSYRKLNKLPLD
>maker-lg2-snap-gene-60.122-mRNA-1
MEVQRNQHISPSSLLASDKEKSALEFQATSEDVPVIDLSNPNEDFLARTVVKVSEEWGIFKVVNHGIPTELMRRLKEVGTEFFELPETEKEVVARPAGSTNIEGYNTNAYNNKEGVKVWADHLFHTLWPPSRINYKFWPKNSPDYREVNEEYAKHIKKLSEKIMGWLSEGLGLHREALKEGLGGETVEYMMKVIFYPPCPDLDLLYGAPPHTDLNGITCLIANDVHGLQAFKDNNWIDVKYDESVIIIIIADQITRMSNGKYKSGQHRATMDTERTRISWPVFAEPHLDHVVGTLPELTTGDDEKAPKFKPYVYRDYKYLINPICFYQFTVLPCVGCVTEGIRACCIKRRWIRSEQAWPTRMTWPVLVNPNPGLVVGPLVELTGDDDPPKFESFYFHFFGRPQIALEENDVEHNICHYVVLKC
>maker-lg2-snap-gene-51.171-mRNA-1
MELPVVDLSRYLESSGPETDLLDSCREVSRILRETGALLVKDPRCTAQDNDRFIDMMETYFQMPDDFKRLQQRPNLHYQVGVTPEGVEVPRSLVDEEMQEKLNAMPEEYKPHTPKGPDHKWRYMWRAGPRPSNTRFKELNSEPVIPQGFPEWKEVMDSWGYKMISTVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYDEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPLGCLLIQTGKQIEWLTGGECIAGMHEVVVTSKTIDAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFAESSLASNYPAIPAGEYVEQELSVINLKGKKSRS
>maker-lg3-snap-gene-71.210-mRNA-1
MGLERDQETKQSSHIPVVDLSNPDQELVARAVVKASEEWGVFQLVNHGIPTELRQRLQNVGKQFFELPDAEKEAMAKPEYSREGYARRYEFDLEKKIGTVDQLFNIIWPPSSVDYKYWPKNLQDYRKVNEEYATHVRMLSEKIMEWLSEGLGLQREAIKEVIAGEYMLIVNYYPPCPHSDLIEGLDAHTDVSGLTLILPNEIPGLQVLKDDHWIELENIPSAIIVLIADQILRLSNGRYKSVLHKTTVDKERTRMSWPVLVRPTSDTVVGPFPELIGQDDPPKFKPMAYKDYIYRKVRNLSFVDLDST
>snap_masked-lg3-processed-gene-71.75-mRNA-1
MVDERDLDDMQSSHIPVVDLNNPDQELVAHAVVKASEEWGIFQVVNHGIPSELIQRLQNVGRQFFELPDVEKETVAKPADSREGYKRTNELDLKKKRGTIYKLLHNIWPPSSVNYTYWPKNPQDYREVNEEYTRQMKMLSEKIMEWLSEGLGLRREAFKDVMREYMMIVNYYPPCPHPDLIQGLEPHTDTSGFTLLLPNEVPGLQVFRDDHWIDFEYIPSAIIVVIGDQMLRLSNGRYKNVLHKTTVDKERIRMSWPVLVKPTYDTVIEPFAELTGQNDPPKFKPIAYKDYIYRKFSKLPMISTDK
>snap_masked-lg3-processed-gene-71.78-mRNA-1
MVLERDQDIQSSHIPVVDLSNPDQELVAHAVVKASEDWGIFQVVHHGIPTELIQRLQNVGKQFFELPDAEKEAVAKPVNSREGYVRRELDLENKIGTIYHLSHNIWPPSSVNYTYWPKNPQDYREVNEEYTRQMKMLSEKIMEWLSEGLGLQREAVKDVMGDYMMVVNYYPPCPHSDLIEGLETHTDISGFTLLQPNEVPGLQVFKDDHWIELEYVPSAIIVVIGDQLLRLSNGRYKNVLHKTTVDKERTRMSWPLLVRPTYDTVIRPFPELTSHDDPPKFKPISYKDYVYRKFSKLPIDVMCEFDLSKTLIILKVIRPFPELTGHDNPPKFN
>snap_masked-lg3-processed-gene-71.80-mRNA-1
MGWLSEGLGLHREALKEGLGGETVEYLMKVIFYPPCPKMDLLFGAPPHTDPNGITFLIADQVHGLQAFQDNKWIDVEYNDSVIVVIIADQITRMSNGRYKSGEHRATMDTVKTRISWPAFAEPNLDHVVGTFPELITGDDENAPKFKPYDYREFKFRKMNKLPID
>snap_masked-lg6-processed-gene-108.60-mRNA-1
MVACWPEPIVSVQALSQTGVLTVPSRYVKPLHQRPVFSSDQTEADVEIPVVDMSGVWGKQERLRHVRSACEEWGFFQVVNHGVSHGLMERMRGAWRDFFELPLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSVRNPSKWPAQPPKIRFLQSY
>maker-lg6-snap-gene-97.149-mRNA-1
MSVELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSGDMYQKLRRLSGGVFGLRDEEKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDRPRDEESS
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University