Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Diptychocarpus strictus
Species name
Diptychocarpus strictus is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Diptychocarpus_strictus.FLS.fa
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>Distr.0002s80000.1.v2.1
MEIERVQDISSSSLLREAIPFEFIRSEKEQPAITTFRGPTPAIPVVDLSNPDEESLLRAVVKASEEWGIFQVVNHGIPTELIRRLQEVGRKFFELPSSEKESVAKPVDSKDIEGYGTKLQKDLEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYALHVKKLSETLLGFLSQGLGLRRDALKEGLGGDTAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGKYKNVLHRTTVDKEKTRMSWPVFLEPHREVVVGPLPELTNDDNPPKYKPFAFKDYSYRKLNKLPLD
>Distr.0004s174600.1.v2.1
MVVERDQEIPSTSNLSKKDEPATTNVPVVDLSDPDQDLVARTVVKASKDWGVFQVVNHGIPIQLIQRVQKVGMQFFDLPEAEKNVLARLPGSIEGYFARRVELNIEKRKGIVDQFYHNVWPPSCVNYKFWPKNPPYYREVTEEYARQVKMLSEKIMEWLSEGLGLQSEAIKEVIGLEYVMNINYYPPCPHFDLIEGLDPHTDINGLTLLHTNEIPGLQVFKDDKWIEVEYIPSALIVNIGDQITMLSNGKYKNVLHKTTVDKVKTRMSWPVILRPTDDTVVGPFLELTSDDDPPKFKPISYKDYAYSKLPFLNMDS
>Distr.0004s174100.1.v2.1
METEIEKSANIASLYIPVIDLSNPDEELVARAIVKASKEWGVFQVLNHGIPTELISRLQEVGTKFFELPETEKEAVARPADSIDIEGYRTKYQKDIEGRDPWVDHHFHRISPPSKVSYTFWPKNPPEYREVNEEYAIHIKKLSEKTMEWLSEGLGLRPEALKEGLGGDTVEYLMKINYYPPVLDQELVVVGVPDHTDYNGLTLIVANDLLGLQAFKDNQWIDAKYSNSGMIIIIGDQFHRMSNGKYKSVAHRVTMNKEKMRISWAVFVESNLDHVFGTLPELIASNDDDEPKFKPYAYKDYKFRKLKKLPLY
>Distr.0004s174000.1.v2.1
MYVETIPIIDLSDLDKELVTRAVVKASQEWGFFHVVNHGIPTDLIERFKEVGKQFFELPEIEKKAVEKRDGSGEYEGYTTSHQFQYEDGPQIWTDNLFHRLWPSSIINFNYWPKNPPQYREVTEEYTKETKKLSERLLGVLSEGLGLQHEALHEGLGGELVEYALRINSFSPNPHPYSTLGLSEHTDFIAMTLIITNEVPGLQIFKDDRWIDVQYIPFAINVTIGDQILRLSNGKYKNVLHRVTVDKEKQRISWPVFVDANPHVIIGPLPELITDDSPSKFKPITCEEFKHRSLFKLPID
>Distr.0004s175000.1.v2.1
MTKKDQPVITQVPVVDLSNPDQKLVARAVANACQEWGVLQVVNHGIPTELITRLQNVGRAPRNREGNRGETSSYPSRRILEEVSIRSRKWSRHC
>Distr.0004s71000.1.v2.1
MENHTQEHDSTIEVSPLNCIDLADDDLHLSVASLKQACLDSGFFYVTNHGINEEFMEEVFEQSKRFFALPLAEKMKVLRNEKHRGYTPVLDEILDPENQINGDHKEGYYIGIEVPKDDPHWDKPFYGPNPWPDSDVLPCWRETMEKYHQEALRVSKAIARLLALALDLDVDYFDSQEMLGKPIATMRLLHYEGVSDPSKGIYACGAHSDYGMMTLLATDGVMGLQICKDKNVKPQKWEYVPPIKGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHECLVECLPTCKSESNLPKYPPIKCSTYLAQRYKETHVELSIYE
>Distr.0004s174400.1.v2.1
MTEKDQPVITQVPVVDLSNPDQKLVARAVANACQEWGVVQVVNHGIPTELITRLQIVGRQFFELPETEKETVAKPAGLKREGYARRVELDLENKQGIVDQLMQNTWPPSCVNYRFWPKNPPDYREVYEEYTKEVKVLMEKIMEWLSEGLGLRCEAINEVIGGKYMINTNYYPPCPHLDLVEGLDPHKDVNGLSIVLTNEIPGLQVFKDDKWIDMEYIPSALIVIIGDQITMLSNGKYKNVLHKTIVDKEKTRMSWPLMVMPTYDTVVGPFSELTSDDDPPKFKPISYKDYLHRRMAGIGI
>Distr.0004s174900.1.v2.1
MAERDKDLLHLSSNMTEKDQLVITQVPVVDLSDPDQDLVARKVVKACQEWGAFQVVNHGISTELITRFQNVGRQFFELTETEKEAVAKPARIHPEGYWRRFQLDLESGQGTVEQLIHYVWPPSCVNYRYWPTDPLDYRKVNEEYASEAKKLLEKIMGWLSEGLGLRCDAIKEVTGGKYMINNNYYPPCPHLDLVDGLDPHTDVNGLTLLLTNEIPGLQVFKDDNWINLAYIPSALIVLIGDQIQILSNGKYKNVLHKTTVDKEKTRMSWPFMAMPAHDMVIQPFPELTSNEDPPKFEPIGYKDYVHRKFASFGIHDHN
>Distr.0004s174300.1.v2.1
MMVSSDLHIPVVDLSNSDEELVARAVVKATKEWGVFQVVNHGIPVDLIRRLQIAGKQFFELPAAEKEAVVKPDGFMEGFSRRFQEDVEKGQGTVDKLFHNIWPPSSVNYRYWPTNPPDYREVNEEYARQVKKLCEKILEWLSEGLGLRREVLKEIIGSVYMMNSNYYPPCPHSDFMKGLPQHTDTNALTLIVSNEVPGLQVFKDDHWIYVEYIPSAVIVNIGDLIMMLSNGRYKNVLHKSTVDKERIRISLTVLVKPNYDMVVGPLPELTSDEDPPKFIPISYRDYLKQELPHLSTIQNV
>Distr.0004s174700.1.v2.1
MVVKKCEEISSSSNISEKDKPEIPQVPVIDLSNPDQELVARAMVKASKDWGVFQLVNHGIPSELIQRLQKVGMQFFELPEEEKNVLARQAGSIEGYFARRVELNIEKRKGIVDQIYQRVSPPSCVNYKYWPKNPPDYREVTEEYSKQVKMLSEKIMEWLSEGLGLQCEAIKEVIGLEYMMNINYYPPCPHFDLIEGLDPHTDINGLTLLLTNEIPGLQVFKDNYWIKVEYIPNAVIVNIGDQITMLSNGKYKNVLHKTTVDKVKTRMSWPFLVRPSYDTVVGPFLELTSDDDPPKFKPISYKDYSYRKLAFLNTDS
>Distr.0011s7400.1.v2.1
MELPVVDLSRYLEFSGGEPACQLGTDLLDSCGEVSRILRETGALLVKDPRCSAEDNDRFIDMMETFFQMPDDFKRLQERPNLHYQVGVTPEGVEVPRSLVDENMQETFKKMPKEYKPHIPKGPDHKWRYMWRAGPRPLNTRFKELNSEPVIPQGFPEWKEIMDSWGHKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTIDAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFAESSLASNYPAITAGEYVEQELSVINLKGNKGLS
>Distr.0042s9300.1.v2.1
MEVKKDQRHISPPSLPTKTIPVFDLGDPDDDLVVSSLVKASQEWGAFQVVNHGIPPELIKRLQEVGKEFFQLSAAEKEAVADSKSFEGYEAQDLKQVKAWNDHLLHKIWPPTCINYKHWPKNPPDYREVTEEYAKNVTRLSETIMGCLSKGLGLPREALKEGLGCPEYMLRINYYPPLDSVIGAPAHSDLAALALLVSNEVPGLQVYKDNQWFDVEYINSAVVVLIGDQIMRMSNGKYKNVLHRSTMDMMKMRMTWPVLVNPGPGWVVGPLPELTGGKNPPKFESLTFEDYVYRKSKNLLPLSG
MEIERVQDISSSSLLREAIPFEFIRSEKEQPAITTFRGPTPAIPVVDLSNPDEESLLRAVVKASEEWGIFQVVNHGIPTELIRRLQEVGRKFFELPSSEKESVAKPVDSKDIEGYGTKLQKDLEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYALHVKKLSETLLGFLSQGLGLRRDALKEGLGGDTAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGKYKNVLHRTTVDKEKTRMSWPVFLEPHREVVVGPLPELTNDDNPPKYKPFAFKDYSYRKLNKLPLD
>Distr.0004s174600.1.v2.1
MVVERDQEIPSTSNLSKKDEPATTNVPVVDLSDPDQDLVARTVVKASKDWGVFQVVNHGIPIQLIQRVQKVGMQFFDLPEAEKNVLARLPGSIEGYFARRVELNIEKRKGIVDQFYHNVWPPSCVNYKFWPKNPPYYREVTEEYARQVKMLSEKIMEWLSEGLGLQSEAIKEVIGLEYVMNINYYPPCPHFDLIEGLDPHTDINGLTLLHTNEIPGLQVFKDDKWIEVEYIPSALIVNIGDQITMLSNGKYKNVLHKTTVDKVKTRMSWPVILRPTDDTVVGPFLELTSDDDPPKFKPISYKDYAYSKLPFLNMDS
>Distr.0004s174100.1.v2.1
METEIEKSANIASLYIPVIDLSNPDEELVARAIVKASKEWGVFQVLNHGIPTELISRLQEVGTKFFELPETEKEAVARPADSIDIEGYRTKYQKDIEGRDPWVDHHFHRISPPSKVSYTFWPKNPPEYREVNEEYAIHIKKLSEKTMEWLSEGLGLRPEALKEGLGGDTVEYLMKINYYPPVLDQELVVVGVPDHTDYNGLTLIVANDLLGLQAFKDNQWIDAKYSNSGMIIIIGDQFHRMSNGKYKSVAHRVTMNKEKMRISWAVFVESNLDHVFGTLPELIASNDDDEPKFKPYAYKDYKFRKLKKLPLY
>Distr.0004s174000.1.v2.1
MYVETIPIIDLSDLDKELVTRAVVKASQEWGFFHVVNHGIPTDLIERFKEVGKQFFELPEIEKKAVEKRDGSGEYEGYTTSHQFQYEDGPQIWTDNLFHRLWPSSIINFNYWPKNPPQYREVTEEYTKETKKLSERLLGVLSEGLGLQHEALHEGLGGELVEYALRINSFSPNPHPYSTLGLSEHTDFIAMTLIITNEVPGLQIFKDDRWIDVQYIPFAINVTIGDQILRLSNGKYKNVLHRVTVDKEKQRISWPVFVDANPHVIIGPLPELITDDSPSKFKPITCEEFKHRSLFKLPID
>Distr.0004s175000.1.v2.1
MTKKDQPVITQVPVVDLSNPDQKLVARAVANACQEWGVLQVVNHGIPTELITRLQNVGRAPRNREGNRGETSSYPSRRILEEVSIRSRKWSRHC
>Distr.0004s71000.1.v2.1
MENHTQEHDSTIEVSPLNCIDLADDDLHLSVASLKQACLDSGFFYVTNHGINEEFMEEVFEQSKRFFALPLAEKMKVLRNEKHRGYTPVLDEILDPENQINGDHKEGYYIGIEVPKDDPHWDKPFYGPNPWPDSDVLPCWRETMEKYHQEALRVSKAIARLLALALDLDVDYFDSQEMLGKPIATMRLLHYEGVSDPSKGIYACGAHSDYGMMTLLATDGVMGLQICKDKNVKPQKWEYVPPIKGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHECLVECLPTCKSESNLPKYPPIKCSTYLAQRYKETHVELSIYE
>Distr.0004s174400.1.v2.1
MTEKDQPVITQVPVVDLSNPDQKLVARAVANACQEWGVVQVVNHGIPTELITRLQIVGRQFFELPETEKETVAKPAGLKREGYARRVELDLENKQGIVDQLMQNTWPPSCVNYRFWPKNPPDYREVYEEYTKEVKVLMEKIMEWLSEGLGLRCEAINEVIGGKYMINTNYYPPCPHLDLVEGLDPHKDVNGLSIVLTNEIPGLQVFKDDKWIDMEYIPSALIVIIGDQITMLSNGKYKNVLHKTIVDKEKTRMSWPLMVMPTYDTVVGPFSELTSDDDPPKFKPISYKDYLHRRMAGIGI
>Distr.0004s174900.1.v2.1
MAERDKDLLHLSSNMTEKDQLVITQVPVVDLSDPDQDLVARKVVKACQEWGAFQVVNHGISTELITRFQNVGRQFFELTETEKEAVAKPARIHPEGYWRRFQLDLESGQGTVEQLIHYVWPPSCVNYRYWPTDPLDYRKVNEEYASEAKKLLEKIMGWLSEGLGLRCDAIKEVTGGKYMINNNYYPPCPHLDLVDGLDPHTDVNGLTLLLTNEIPGLQVFKDDNWINLAYIPSALIVLIGDQIQILSNGKYKNVLHKTTVDKEKTRMSWPFMAMPAHDMVIQPFPELTSNEDPPKFEPIGYKDYVHRKFASFGIHDHN
>Distr.0004s174300.1.v2.1
MMVSSDLHIPVVDLSNSDEELVARAVVKATKEWGVFQVVNHGIPVDLIRRLQIAGKQFFELPAAEKEAVVKPDGFMEGFSRRFQEDVEKGQGTVDKLFHNIWPPSSVNYRYWPTNPPDYREVNEEYARQVKKLCEKILEWLSEGLGLRREVLKEIIGSVYMMNSNYYPPCPHSDFMKGLPQHTDTNALTLIVSNEVPGLQVFKDDHWIYVEYIPSAVIVNIGDLIMMLSNGRYKNVLHKSTVDKERIRISLTVLVKPNYDMVVGPLPELTSDEDPPKFIPISYRDYLKQELPHLSTIQNV
>Distr.0004s174700.1.v2.1
MVVKKCEEISSSSNISEKDKPEIPQVPVIDLSNPDQELVARAMVKASKDWGVFQLVNHGIPSELIQRLQKVGMQFFELPEEEKNVLARQAGSIEGYFARRVELNIEKRKGIVDQIYQRVSPPSCVNYKYWPKNPPDYREVTEEYSKQVKMLSEKIMEWLSEGLGLQCEAIKEVIGLEYMMNINYYPPCPHFDLIEGLDPHTDINGLTLLLTNEIPGLQVFKDNYWIKVEYIPNAVIVNIGDQITMLSNGKYKNVLHKTTVDKVKTRMSWPFLVRPSYDTVVGPFLELTSDDDPPKFKPISYKDYSYRKLAFLNTDS
>Distr.0011s7400.1.v2.1
MELPVVDLSRYLEFSGGEPACQLGTDLLDSCGEVSRILRETGALLVKDPRCSAEDNDRFIDMMETFFQMPDDFKRLQERPNLHYQVGVTPEGVEVPRSLVDENMQETFKKMPKEYKPHIPKGPDHKWRYMWRAGPRPLNTRFKELNSEPVIPQGFPEWKEIMDSWGHKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTIDAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFAESSLASNYPAITAGEYVEQELSVINLKGNKGLS
>Distr.0042s9300.1.v2.1
MEVKKDQRHISPPSLPTKTIPVFDLGDPDDDLVVSSLVKASQEWGAFQVVNHGIPPELIKRLQEVGKEFFQLSAAEKEAVADSKSFEGYEAQDLKQVKAWNDHLLHKIWPPTCINYKHWPKNPPDYREVTEEYAKNVTRLSETIMGCLSKGLGLPREALKEGLGCPEYMLRINYYPPLDSVIGAPAHSDLAALALLVSNEVPGLQVYKDNQWFDVEYINSAVVVLIGDQIMRMSNGKYKNVLHRSTMDMMKMRMTWPVLVNPGPGWVVGPLPELTGGKNPPKFESLTFEDYVYRKSKNLLPLSG
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University