Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Dichanthelium oligosanthes
Species name
Dichanthelium oligosanthes is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Dichanthelium_oligosanthes.HI4_OMT.fa
Download
>rna-gnl|WGS:LWDX|Do029867.1_mrna
MVRGSAQGDHKISEMEDIQNVFMMYINGVERGINEWKRIFSDAGFSDDYKIMPVLGPFSVIEIYPA
>rna-gnl|WGS:LWDX|Do028446.1_mrna
MAFFAEYSSQELLQAQLQLWHQSLGFFKSVALSIALDLRIADAIHSLGGAATVPQILAEAGINPCKLRDLRRVMRVLTVSGIFSVQQPSTATASSDCKHGAEIYKLTAASRLLVRDTISTMGQQPPFLSVQLMLETCRGCPASRGIHAWFHQDQQQPGLTPFALANNGQTIWERAEHDDAACPFNDAMASDTAFLMPIVLKECGEVFHGLTSLVDVAGGFGGAAATIAAAFPDLKCTVLDLPQVVAKAPSGTNVQYVAGDMFENIPPANAVFLKWIMHDWGDDECVKILKNCKQAIPPRDAGGKIIIIDMVVGSGPSDIKLVETQVLYDLLIMNFNGVERDEQDWKKIFFEAGFKDYKIIPILGVRSIIELYP
>rna-gnl|WGS:LWDX|Do030068.1_mrna
MSPQDLLVALSELQNHLLGYVKSMALKCAVDLGIPDAIHRRGGVATLADIATDTKVHPAKVPDLQRIMQLLSTSGIFSNTAGEDGAEVYGLTTACRFLVGRCNLSPMVPFLVSPLVVSSFFSISDWFKKEPAAAGSLFELAHGCSQWETASKDAGFNRVLEDSMTADSQLFLEVIILDKGRIFRGLSSLVDVGGGHGAATQIIARAFPHIKCTVLDLPHVVSQATASDANVHFVAGDMFGSIPSADAILLKVSIYVMSFWYTGQSSH
>rna-gnl|WGS:LWDX|Do025466.1_mrna
MTLRLLAEMSPQDLLVALSELQNHLLGYVKSMALKCAVDLGIPGAIHRRGGVATLADIATDTEVHPAKVPDLQRMMQLLTTSGIFSNTAGEDGSQVYGLTTACRFLVGRCNLSPMVPFLVSPLVVSSFFGMSDWFRKEPAAAGSLFELAHGCSRWEMASKDAGFSRVLEDSMAADSQLFLEVIIFDKGRIFRGLSSLVDVGGGHGAATQVIARAFPRIKCTVLDLPHVVSQATASDGNVHFVAGDMLESIPPADAVLLKNILHDWRDEDCVKILQRCKQAIPARNAGGKVIIIDMVRGSAQGDGKISEMEDIQNVFMLHINGVERGVNEWKRIFADAGFSEDYKIMPVLGPLSVIEVYPA
>rna-gnl|WGS:LWDX|Do013848.1_mrna
MMNHVLDPFRDSPLSMGLTAWFQHEKPDQSPYTLFEMCGSDDAVNASINNAMAADSRFLMQVVLKECGEIFHGIDSLVDVGGGLGGAAAAMATAFPSLKCSVLDLPHVVAKAPPFSNEQFVAGDMFESIPPTNVIFLKYVLHDWGDDKCIKILKNCKQAIPARDAGGNVIIIDIVVGSSPSDIKLLETQVLCDLDIMKVGGVERDEQEWKKM
>rna-gnl|WGS:LWDX|Do014485.1_mrna
MHDWGDSERVTILKNCKKAIPPRDAGGKVRIVDAVVGSGSSAMKHRETQVLYDLYIMVANGIELDEQEWRKIISEADFTPYKIIPVLGVRSIIEVYP
>rna-gnl|WGS:LWDX|Do025399.1_mrna
MGLSKEQHRSADQQAVLDSQVQLWHHTFGYVKSMALKAALDLGLPDAIHQNGGSATLQQIVTKVTLHPSKIPCLRRLMRVLTVNGVCASLG
>rna-gnl|WGS:LWDX|Do007863.1_mrna
LKQTKEAISTRGPKGKAIIIDVVIGSPSKAQLCLDLLMMAVTTGKQREEIWHRIFLDAGSTRCKIHPVLGVSITH
MVRGSAQGDHKISEMEDIQNVFMMYINGVERGINEWKRIFSDAGFSDDYKIMPVLGPFSVIEIYPA
>rna-gnl|WGS:LWDX|Do028446.1_mrna
MAFFAEYSSQELLQAQLQLWHQSLGFFKSVALSIALDLRIADAIHSLGGAATVPQILAEAGINPCKLRDLRRVMRVLTVSGIFSVQQPSTATASSDCKHGAEIYKLTAASRLLVRDTISTMGQQPPFLSVQLMLETCRGCPASRGIHAWFHQDQQQPGLTPFALANNGQTIWERAEHDDAACPFNDAMASDTAFLMPIVLKECGEVFHGLTSLVDVAGGFGGAAATIAAAFPDLKCTVLDLPQVVAKAPSGTNVQYVAGDMFENIPPANAVFLKWIMHDWGDDECVKILKNCKQAIPPRDAGGKIIIIDMVVGSGPSDIKLVETQVLYDLLIMNFNGVERDEQDWKKIFFEAGFKDYKIIPILGVRSIIELYP
>rna-gnl|WGS:LWDX|Do030068.1_mrna
MSPQDLLVALSELQNHLLGYVKSMALKCAVDLGIPDAIHRRGGVATLADIATDTKVHPAKVPDLQRIMQLLSTSGIFSNTAGEDGAEVYGLTTACRFLVGRCNLSPMVPFLVSPLVVSSFFSISDWFKKEPAAAGSLFELAHGCSQWETASKDAGFNRVLEDSMTADSQLFLEVIILDKGRIFRGLSSLVDVGGGHGAATQIIARAFPHIKCTVLDLPHVVSQATASDANVHFVAGDMFGSIPSADAILLKVSIYVMSFWYTGQSSH
>rna-gnl|WGS:LWDX|Do025466.1_mrna
MTLRLLAEMSPQDLLVALSELQNHLLGYVKSMALKCAVDLGIPGAIHRRGGVATLADIATDTEVHPAKVPDLQRMMQLLTTSGIFSNTAGEDGSQVYGLTTACRFLVGRCNLSPMVPFLVSPLVVSSFFGMSDWFRKEPAAAGSLFELAHGCSRWEMASKDAGFSRVLEDSMAADSQLFLEVIIFDKGRIFRGLSSLVDVGGGHGAATQVIARAFPRIKCTVLDLPHVVSQATASDGNVHFVAGDMLESIPPADAVLLKNILHDWRDEDCVKILQRCKQAIPARNAGGKVIIIDMVRGSAQGDGKISEMEDIQNVFMLHINGVERGVNEWKRIFADAGFSEDYKIMPVLGPLSVIEVYPA
>rna-gnl|WGS:LWDX|Do013848.1_mrna
MMNHVLDPFRDSPLSMGLTAWFQHEKPDQSPYTLFEMCGSDDAVNASINNAMAADSRFLMQVVLKECGEIFHGIDSLVDVGGGLGGAAAAMATAFPSLKCSVLDLPHVVAKAPPFSNEQFVAGDMFESIPPTNVIFLKYVLHDWGDDKCIKILKNCKQAIPARDAGGNVIIIDIVVGSSPSDIKLLETQVLCDLDIMKVGGVERDEQEWKKM
>rna-gnl|WGS:LWDX|Do014485.1_mrna
MHDWGDSERVTILKNCKKAIPPRDAGGKVRIVDAVVGSGSSAMKHRETQVLYDLYIMVANGIELDEQEWRKIISEADFTPYKIIPVLGVRSIIEVYP
>rna-gnl|WGS:LWDX|Do025399.1_mrna
MGLSKEQHRSADQQAVLDSQVQLWHHTFGYVKSMALKAALDLGLPDAIHQNGGSATLQQIVTKVTLHPSKIPCLRRLMRVLTVNGVCASLG
>rna-gnl|WGS:LWDX|Do007863.1_mrna
LKQTKEAISTRGPKGKAIIIDVVIGSPSKAQLCLDLLMMAVTTGKQREEIWHRIFLDAGSTRCKIHPVLGVSITH
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University