Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Crambe hispanica
Species name
Crambe hispanica is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Crambe_hispanica.FLS.fa
Download
>Crahi.0001s0457.1.v1.1
MEIERVQDISSSSLHTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVARAVVKASEEWGIFQVVNHGIPTELIKRLQEVGRTFFELPSAEKESVAKPVDAKDIEGYGTKLQKEVEGKKAWVDHLFHRIWPPSCVNYSFWPKNPPEYREVNEEYALHVKKLSETLLGVLSEGLGLRREALREGLGGDLAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKDRTRMSWPVFLEPHREMIVGPLPELISDDNPPKYKPFAFKDYSYRKLNKLPLD
>Crahi.0005s0175.1.v1.1
MEMETIPVVDLSNPKEDLVARAVVKASQEWGIFHVANHGISPELIRRLKELGTEFHELPETEKKAVAKPQDATDMEGYTTNLKDWADHLFHRIWPPSRINYRFWPKNSQDYRAVNEEYAREIKKLSEKIMEWLSVGLGLRREAFKEGLGGETVEYLMKLIFYPPFSDPDLSSGAPPHTDFNGVTFLIANEVHGLQAFKDNHWIDVKYDDSMILVIIGDLFHRMSNGKYKSAEHRATMDTEKTRISWPVFVEPNLDQVVGTLPELISGDDTTAPKFKPYAYKDFKFRLRYGLPLD
>Crahi.0005s0174.1.v1.1
MEAERDQHTSPPSETIPIIDLSDPEDELVVRAVVKASEEWGAFQVINHGIPAELIRRLQEVGKQFFELPAVEKEAVGKLPDSKGIQGYDAKNPKRSVSWSEHILHKIWPQSCIDYRFWPKNPPNYREVNEEYARHLTKLSETIIGCLSKGLGLGREALKEGLGGAEYMLRINYYPQSSDSAIGAEAHTDFAAMALLVSNEVPGLQVFKENHWFDVEYINSAVVVLVGDQIMRMSNGRYKCVLHRATMDKEKTRMTWPVLVDPKGGLVVGPLPELIGDENPPKFESLNFEDYVYRKVNMLLRDG
>Crahi.0030s0031.1.v1.1
MAESVQVTTSTVDIPVVDLSNPNEDLVARAVVKASETWGMFKVVNHGIPTELMRRLKELGTEFFELPEKEKEAVARPAESIDIEGYTTDYKKDGEGRKTWADHLFHRVWPPSRINYRFWPKNSPDYREVNEEYAREIKKLSEKIMGWLSEGLGLHRDALKEGLGGETVEYLMKIIFYPPCPKLDLLYGAPHHTDLNGITFLIADEVDGLQAYQDNKWVDVKYDDSGIVVIIADQIKRMSNGRYKSGEHRATMDTVRTRLSWPVFAEPNLDHVVGPLPELVLDDAPKFKPYVYREYKFLKMNKLPLD
>Crahi.0047s0015.1.v1.1
MRRHGVKQRKKHFSNNANKDMRVMETKTQEEDSIIQESLLTCIDLANSDLHQSALSLKKACLDSGFFYVINHGISTEVMDKAFEQSKKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQVQGDHKEGYYIGSEVPRDDPHWDRPFYGPNPWPNPDVLPGWRETMEKYHQEALRVCKAIAKLLALALDLDVDYFDSPVMLGKPISTLRLLYYEGKSDPSKGIYGTGAHSDYGMITLLATDGVMGLQICKDRNAKPQKWEYVPSIEGAIVVNLGDMMERWSNGLFKSTLHRVLLNGQERYSIPFFVEPSHDCIIECLPTCQSENNLPKFPAIKCSAYLTQRYEESHLKFSSYKK
>Crahi.0056s0027.1.v1.1
MELPVVDLSRYLQSSNGESDSHLGTDLLDSCREVSRILKETGALLVKDPRCSAEDNDRFIDMMETYFSKPDEFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPKEYKPHIPKGPDHKWRYMWRVGPRPSNTRFQELNSEPVIPEGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKDAIKLAKEQNRSIWRVSSTLFAHIASDAELKPLGHFAESSLASNYPAIPAGEYVEQELSVINLKGKKASS
>Crahi.0097s0051.1.v1.1
MEVERGGQYTSPPSETVPIIDLSNPNDELVAHALVKASEEWGAFVVVNHEIPAELMRRFQEVGRQFFELPAVEKEAVAMLRGSKSMEGYDAKNPKLAKSWDDHLFHKIWPQSCIDYKFWPKNPPEYREVTEEYARHVTKLSEKIMGYLSKGLGLGREVLKETLGGGEYMLRINYYPPSDSVIGAPAHTDFDALTLLVSNGVPGLQVFKDNLWLDVEDINSAVVVLLGDEIMRLSNGRYKSVLHRSTMDKEKTRMTWPVLIDPMPGMVVGPLPELIGDENPPKFESLTLEDYMYLRTNMLLGDG
>Crahi.0097s0052.1.v1.1
MEVERDQTVSSPKIPIIDLSNLNKEQVAAAVVKASQEWGVFQVVNHEIPKEVVQSLKKVGTQFFELSSETEKEAVAKPANSKDIQGYVNKSQKDGKDKVDRVDTLVHNLSPPSWVDYRYWPKNPPEYRQVNEEYARYVKKLAERILGWLSEGLGLEREALFNKGFGGGDKAVYTMQINYYPPCRPHANLVLGLHPHTDIHGLTILVPNEIAGLQVFKDDQWFDVVYNPSGILILIGDPIWRMSNGKYKNVLHRTILNKETRMSWVVLLKPTYDMVIEPLMKLTGDGDEAPKFKPITYGEHVYRKVHNLPLE
>Crahi.0097s0053.1.v1.1
METTSAPDEIPIVDLSNPNQDLVARAVVKASEEWGIFHVVNHGVPPELIRRLKEVGTEFFHLPEAEKKALEKPKDSKNMEGYISNSTGWTDHLFHRIWPPSRVNYRFWPNNIQDYKEVNEEYAREIKKLSEKIMGWLSEGLGLGREALKEGLGGETVEYLMKVVFYRQCQDQDVKYGTPRHTDLSGITFLIANQVYGLQAFKDNKWVDVKYDDSRIVVIIADQVHRISNGRYKRSEHRATLDKEKTRISWPVFVDPNLDHVVRPLPELISGDENAPKFKPYTYRDYKFHVWNRFPLD
>Crahi.0263s0008.1.v1.1
MENHTKEELQDPTMQVSSLNCIDLANDDLHHSVVSLKQACLDCGFFYVINHGINEEFMEDVFEQSKKFFALPLEEKMKVLRNEKHRGYTPVLDEILDPENQVNGDHKEGYYIGIEVPKDDPDYDKPFYGPNPWPDSDVLPQWRETMEKYHQEALRVSKDIGRLLALALDLDANYFDTPEMLGKPIATMRLLHYQGVSDPSKGIYACGAHSDYGMMTLLATDGVMGLQICKDKNAKPQKWEYVPAIKGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHECLVECLPTCKSERDVPKYPAIKCSTYLTQRYKETHAELSIYNQEI
MEIERVQDISSSSLHTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVARAVVKASEEWGIFQVVNHGIPTELIKRLQEVGRTFFELPSAEKESVAKPVDAKDIEGYGTKLQKEVEGKKAWVDHLFHRIWPPSCVNYSFWPKNPPEYREVNEEYALHVKKLSETLLGVLSEGLGLRREALREGLGGDLAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKDRTRMSWPVFLEPHREMIVGPLPELISDDNPPKYKPFAFKDYSYRKLNKLPLD
>Crahi.0005s0175.1.v1.1
MEMETIPVVDLSNPKEDLVARAVVKASQEWGIFHVANHGISPELIRRLKELGTEFHELPETEKKAVAKPQDATDMEGYTTNLKDWADHLFHRIWPPSRINYRFWPKNSQDYRAVNEEYAREIKKLSEKIMEWLSVGLGLRREAFKEGLGGETVEYLMKLIFYPPFSDPDLSSGAPPHTDFNGVTFLIANEVHGLQAFKDNHWIDVKYDDSMILVIIGDLFHRMSNGKYKSAEHRATMDTEKTRISWPVFVEPNLDQVVGTLPELISGDDTTAPKFKPYAYKDFKFRLRYGLPLD
>Crahi.0005s0174.1.v1.1
MEAERDQHTSPPSETIPIIDLSDPEDELVVRAVVKASEEWGAFQVINHGIPAELIRRLQEVGKQFFELPAVEKEAVGKLPDSKGIQGYDAKNPKRSVSWSEHILHKIWPQSCIDYRFWPKNPPNYREVNEEYARHLTKLSETIIGCLSKGLGLGREALKEGLGGAEYMLRINYYPQSSDSAIGAEAHTDFAAMALLVSNEVPGLQVFKENHWFDVEYINSAVVVLVGDQIMRMSNGRYKCVLHRATMDKEKTRMTWPVLVDPKGGLVVGPLPELIGDENPPKFESLNFEDYVYRKVNMLLRDG
>Crahi.0030s0031.1.v1.1
MAESVQVTTSTVDIPVVDLSNPNEDLVARAVVKASETWGMFKVVNHGIPTELMRRLKELGTEFFELPEKEKEAVARPAESIDIEGYTTDYKKDGEGRKTWADHLFHRVWPPSRINYRFWPKNSPDYREVNEEYAREIKKLSEKIMGWLSEGLGLHRDALKEGLGGETVEYLMKIIFYPPCPKLDLLYGAPHHTDLNGITFLIADEVDGLQAYQDNKWVDVKYDDSGIVVIIADQIKRMSNGRYKSGEHRATMDTVRTRLSWPVFAEPNLDHVVGPLPELVLDDAPKFKPYVYREYKFLKMNKLPLD
>Crahi.0047s0015.1.v1.1
MRRHGVKQRKKHFSNNANKDMRVMETKTQEEDSIIQESLLTCIDLANSDLHQSALSLKKACLDSGFFYVINHGISTEVMDKAFEQSKKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQVQGDHKEGYYIGSEVPRDDPHWDRPFYGPNPWPNPDVLPGWRETMEKYHQEALRVCKAIAKLLALALDLDVDYFDSPVMLGKPISTLRLLYYEGKSDPSKGIYGTGAHSDYGMITLLATDGVMGLQICKDRNAKPQKWEYVPSIEGAIVVNLGDMMERWSNGLFKSTLHRVLLNGQERYSIPFFVEPSHDCIIECLPTCQSENNLPKFPAIKCSAYLTQRYEESHLKFSSYKK
>Crahi.0056s0027.1.v1.1
MELPVVDLSRYLQSSNGESDSHLGTDLLDSCREVSRILKETGALLVKDPRCSAEDNDRFIDMMETYFSKPDEFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPKEYKPHIPKGPDHKWRYMWRVGPRPSNTRFQELNSEPVIPEGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKDAIKLAKEQNRSIWRVSSTLFAHIASDAELKPLGHFAESSLASNYPAIPAGEYVEQELSVINLKGKKASS
>Crahi.0097s0051.1.v1.1
MEVERGGQYTSPPSETVPIIDLSNPNDELVAHALVKASEEWGAFVVVNHEIPAELMRRFQEVGRQFFELPAVEKEAVAMLRGSKSMEGYDAKNPKLAKSWDDHLFHKIWPQSCIDYKFWPKNPPEYREVTEEYARHVTKLSEKIMGYLSKGLGLGREVLKETLGGGEYMLRINYYPPSDSVIGAPAHTDFDALTLLVSNGVPGLQVFKDNLWLDVEDINSAVVVLLGDEIMRLSNGRYKSVLHRSTMDKEKTRMTWPVLIDPMPGMVVGPLPELIGDENPPKFESLTLEDYMYLRTNMLLGDG
>Crahi.0097s0052.1.v1.1
MEVERDQTVSSPKIPIIDLSNLNKEQVAAAVVKASQEWGVFQVVNHEIPKEVVQSLKKVGTQFFELSSETEKEAVAKPANSKDIQGYVNKSQKDGKDKVDRVDTLVHNLSPPSWVDYRYWPKNPPEYRQVNEEYARYVKKLAERILGWLSEGLGLEREALFNKGFGGGDKAVYTMQINYYPPCRPHANLVLGLHPHTDIHGLTILVPNEIAGLQVFKDDQWFDVVYNPSGILILIGDPIWRMSNGKYKNVLHRTILNKETRMSWVVLLKPTYDMVIEPLMKLTGDGDEAPKFKPITYGEHVYRKVHNLPLE
>Crahi.0097s0053.1.v1.1
METTSAPDEIPIVDLSNPNQDLVARAVVKASEEWGIFHVVNHGVPPELIRRLKEVGTEFFHLPEAEKKALEKPKDSKNMEGYISNSTGWTDHLFHRIWPPSRVNYRFWPNNIQDYKEVNEEYAREIKKLSEKIMGWLSEGLGLGREALKEGLGGETVEYLMKVVFYRQCQDQDVKYGTPRHTDLSGITFLIANQVYGLQAFKDNKWVDVKYDDSRIVVIIADQVHRISNGRYKRSEHRATLDKEKTRISWPVFVDPNLDHVVRPLPELISGDENAPKFKPYTYRDYKFHVWNRFPLD
>Crahi.0263s0008.1.v1.1
MENHTKEELQDPTMQVSSLNCIDLANDDLHHSVVSLKQACLDCGFFYVINHGINEEFMEDVFEQSKKFFALPLEEKMKVLRNEKHRGYTPVLDEILDPENQVNGDHKEGYYIGIEVPKDDPDYDKPFYGPNPWPDSDVLPQWRETMEKYHQEALRVSKDIGRLLALALDLDANYFDTPEMLGKPIATMRLLHYQGVSDPSKGIYACGAHSDYGMMTLLATDGVMGLQICKDKNAKPQKWEYVPAIKGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHECLVECLPTCKSERDVPKYPAIKCSTYLTQRYKETHAELSIYNQEI
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University