Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Corymbia citriodora
Species name
Lemon-scented gum (Corymbia citriodora) is a species of eudicot in the family Myrtaceae (myrtle family).
Species info
Gene sequence info
Corymbia_citriodora.HI4_OMT.fa
Download
>Cocit.A1634.1.v2.1
MNLGDGDGDADADAGELLQAQAHVWNHVFSYITSWCLKCALQLGIPDLIHDHGRPMTLPDLVSALHIRPAKAHCIGRLMRVLAHSGFFQLRDLEEEDRRSGYTLTTVSRLLLKNNPAGPAPFVLGTFHPFFTEPWHHLTTWLEDGEGAAPTIFEMAHGGPTWEVIRRDSEVNGLLSRAMDNDSRLVAKAVVETCRGVFDGLSSVVDVGGESGTMGRAITDAFPRLECTVLDLPNVVDGLVGSERLKFVGGDMFVAIPPADAVLLKWILHNWSDEKCIDILKRSKEAISGAGGVGKVIIIDIVVGNQADDHRSTETQLFWDMLMMATVDAKERGEEEWEKLFVDAGFSGYKIISHLGVRSLIEVYP
>Cocit.E2046.1.v2.1
MHHLGHSDETKEMFQAQSHLYNLTSVFVSSMSLKCAVQLGIPDVINDHKRPITLLELASALQIHPSKTTCLDRLMRVLVHLHIFAETLVPKHGGGGGEEVEAYVLTYVLTPSSSLLLRDKATGLSPFIVAMLDPALMTPWHFLGDWFRGSHESNSSNKLISPFEAAHGSSLWDYGRKNPEFGHAFNGAMAIDSQIMNLVISDCKHIFQGLNTLVDVGGGTGTVAKIISEACQGLKCTVLDLPHVVAGLPESENVKFVGGDMFEFIPPSDAIVLKLMLHAWSDEDCVKVLRKCREAIAGKNERGKVIIIDIVIGETQDDDAKAIATKLFFDMLMMAVVTGRERTEKEWEKLFSEAGFSQHKITPMFGLRSLIEVFP
>Cocit.E2044.1.v2.1
MHPFSRSQEAKEMFQAQSHLYNHTFNFISSMSLKCAVQLRIPDVIYDHKRPMTLIDLASKLRIHPSKATCLYRLMRVLVHSGIFAETVVPRDGKQEEEIKAYVLTPSSCLLLNDKATGLSPFVATMLDPALVSPWHFLGDWLGGNNHEDCRDKLNNPFEAVHGVNIWDYGRKNPEFGNTFNTAMAGDCQLMNLVIKDYKEIFEGLNSLVDVGGGTGTMARIISEVCPGLKCTLLDLPHVVAGLPESESENVKFVGGDMFESIPSADAILLKVCTLGIALL
>Cocit.E2043.1.v2.1
MHHLDHSSETKEMFQVQSHLYNLTSIFVSSMSLKCALQLGIPDVINEHKRPITLLELASALQIHPSKTTCLHRLMRVLVHLHIFAETLVPKHGGGGEEVEAYVLTPSSSLLLSDKATGLSPFIVAMLDPALMTPWHFLGDWFRGSHESNCSNKLISPFEAAHGLNLWDYGRKNPEFGHAFNGAMAIDSQMMNLIIRDCKHIFQGLNTLVDVGGGTGTVAKIISEACQGLKCTVLDFPHVVAGLPESENMKFVGGDMFEFIPPSDAILLKLMLHAWSDEDCVKVLRKCKEAIAGKNERGKVIILDIVIGETQDNDANAIATKLFFDMLMMTVVTGRERTEKEWEKLFLEAGFSQHKITPMFGLRSLIEVFP
>Cocit.F0095.1.v2.1
MFQAQSHLYNLTSVFVSSLSLKCAVQLGIPDVINDHKRPITLLEVASALQIHPSKTTCLDRLMRVLVHLHIFAETLVPKHEGEGEEVEAYVLTPSSILLLRDKTTGLSPFIVAMLDPALMTPWHFLGDWFRGSHESNCSNKLISPFEAAHGLNLWDYGRKNPEFGHAFNGAMAIDSQMMNLVIRDCKHIFQGLNTLVDVGGGTGTVAKLISEACQGLKCTVLDLPHVVAGLPESENVKFVGGDMFEFIPPSDAILLKLMLHAWNDEDCVKVLRKCREAIAGKNERGKVIIIDIVIGETQDDDAKAIATKLFFDMLMMTVVTGRERTEKEWEKLFSEAGFSQHKITPMFGLRSLIEVFP
>Cocit.F0094.1.v2.1
MSMKLYQRLLYRTRNFSLYYSNRVTPDSSSLVHSKAVHHLSHSHEAKEMFQAQSHLYNHTFNFISSMSLRCAVQLHIPDIIHDHKQPMTLLELASVLQIHPSKSTCLYRLMRVLVHSGIFTETLVLKNGEEEEEKTKAYVLTPSSRLLLSDKVVGVSPFVAAMLDPALVTPWHFLGDWLQGNNDIDCRDKSINPFMAAHGVDLWGYGRKNPEFGNTFNRAMASDSQMMNLVIQDCKEIFEGLN
>Cocit.H1843.1.v2.1
MNLGNGNGDATASELLRAQAHIWNHIFSFINSMCLKCAIDLGIPDLIHHHGRPMTLLELVSALHIQPTKAHCIHRLMRILIHSGFFQLRDLEEADGQTGYVLTRASCLLVKPNPSSVAPFVLLALTPFSMAPWQYLSAWLREGEDVHSSTFEMANGVPAWDAWRSPEVDGLFDEAMARDSSLMAKAVVDTCGGVFEGLSSVVDVGGGTGTMGKAIMEAFPHLECTVFDQPHVVDGLVGSERLKFVGGDMFVEIPSADAVFLKWILHDWSDEKCIEILKRSKEAVSGRGKVGKVIMIDMVVGNQTEDHRSTETQLFFDMLMMVNLNGKERSEKEWKKLFVDAGFRGYKIISRLGLRSLVEIYP
>Cocit.H1841.1.v2.1
MGKAIKEAFPHLECTVFDQPHVVDGLVGSERLKFVGGDMFVEIPPADVVFLKWILHDWSDEKCIEILKRSKEAVSGRGKVGKVIMIDMVVGNQTEDRRSTETQLFFDMMMANLNGKERSEKEWEKLFVDAGFSIYKIVSRLGLRSLVEIYP
>Cocit.K2190.1.v2.1
MQKAMSIKLNQRLLYRIGNFSLYYPNRVTPDSSSLAHSKTVHNLIHTHEAKKMFQAQSRLYNHTFNFISSMLLRCAVQLRIPDIIHDHKQPMTLPELASVLQIHPSKSTCLYRLMRVLVHLDIFTETLVLKNGEEEEEGGGKTKAYVLTPSSRLLLSDEVVGVSPFVAAMLDPALVTPWHFLGDWLQGSNVNDCRDKLINPFMAAHGVDIWGYGKKNPKFGNTFNRAMASDSQMMNLVIQDCKEIFEGLNSLVDVGGGMGIVARIISEACPGLKCTVLDLPQVVAGLPDSENVKFVGGDVFESIPSTDAILLKWMLHGWSDEDCVKVLRKCKEAIGDKNNDQGKVIIMDIVIDETEDNAQETATKLFFDMLMMTVATGRERTKKEWEKLFLEAGFKQHKITPMFGLKSLIEVFP
>Cocit.L5242.1.v2.1
MTMEEGVLDMNLGNGDGDATTSDLLRAQAHIWNHIFSFINSMCLKCAIDLGIPDLIHHHGRPMTLLELVSVLHIQPTKAHCIHRLMRILTHSGFFELQDLEEADRQTGYVLTCASRLLVKHNPLSAAPFALMALGPFSMGQWQYLSSWLRDAEDVHPSTFEMAYGLPMWEFGGRNPKVVSLFDEAMACDSSLMANVVVHTCGAVFEGLSLVVDVGGGTGTMAKAITEAFPHLECTVFDQPHVVDGLVGRERLKFVGGDMFVEIPPADAIFLKWILHNWSDEKCIEILKRSKEAVSNQGKVGKVIMIDMVVGNQIGDHRSTETQLFFDMLMMVNLNGKERSEKEWEKLFIDAGFSDYKIVSRLGLRSLIEIYP
>Cocit.L5244.1.v2.1
MHVVRGSRITEKGVFDMNLGNGDGGATASELLQAQAHIWNHTYSFINSVCLKWAIEQGIPDVIHHHGRPMTLLELVSALHVQPTKAHYIRRLMRILVHSGFFELRDLEEADHQTGYALTCVSRLLVKHNPSSMAPFALFALTPFPKGHWQYLSAWLRDGEDVHPSTFEMAYGVPTWDAWRSPEVNSLFNQAMASDSSLMAKVVVDTCGGVFEGLSSVVDVGGGTGTMGKAITAAFPHLECTVFDQPHVVDGLVGSERLKFVGGDMFVEIFPADAILLKWILHDWSDEKCIEILKRCREAVSSRGKVGKVIMIDMVVGNQIEDHRSVETQFFFDLLMMVTFNAKERDEKEWAKLFADAGFSGYKIVSCLGLRSLIEIYP
>Cocit.L3140.1.v2.1
MEEGVLDMNLGNGDGDATTSDLLRAQAHIWNHIFSFINSMCLKCAIDLGIPDLIHHHGRPMTLLELVSVLHIQPTKAHCIHRLMRILTHSGFFELQDLEEADRQTGYVLTCASRLLVKHNPLSVAPFALMALGPFSMGQWQYLSAWLRDAEDVHPSTFEMAYGLPMWEFGGRNPEVVGLFDEAMACDSSLMANVVVHTCGAVFEGLSLVVDVGGGTGTMAKAITDAFPHLECTVFDQPHVVDGLVGRERLKFVGGDMFVKIPPADAIFLKVSTMPLCCISNDIGSTWLQFKSTNRKFLFLRYT
MNLGDGDGDADADAGELLQAQAHVWNHVFSYITSWCLKCALQLGIPDLIHDHGRPMTLPDLVSALHIRPAKAHCIGRLMRVLAHSGFFQLRDLEEEDRRSGYTLTTVSRLLLKNNPAGPAPFVLGTFHPFFTEPWHHLTTWLEDGEGAAPTIFEMAHGGPTWEVIRRDSEVNGLLSRAMDNDSRLVAKAVVETCRGVFDGLSSVVDVGGESGTMGRAITDAFPRLECTVLDLPNVVDGLVGSERLKFVGGDMFVAIPPADAVLLKWILHNWSDEKCIDILKRSKEAISGAGGVGKVIIIDIVVGNQADDHRSTETQLFWDMLMMATVDAKERGEEEWEKLFVDAGFSGYKIISHLGVRSLIEVYP
>Cocit.E2046.1.v2.1
MHHLGHSDETKEMFQAQSHLYNLTSVFVSSMSLKCAVQLGIPDVINDHKRPITLLELASALQIHPSKTTCLDRLMRVLVHLHIFAETLVPKHGGGGGEEVEAYVLTYVLTPSSSLLLRDKATGLSPFIVAMLDPALMTPWHFLGDWFRGSHESNSSNKLISPFEAAHGSSLWDYGRKNPEFGHAFNGAMAIDSQIMNLVISDCKHIFQGLNTLVDVGGGTGTVAKIISEACQGLKCTVLDLPHVVAGLPESENVKFVGGDMFEFIPPSDAIVLKLMLHAWSDEDCVKVLRKCREAIAGKNERGKVIIIDIVIGETQDDDAKAIATKLFFDMLMMAVVTGRERTEKEWEKLFSEAGFSQHKITPMFGLRSLIEVFP
>Cocit.E2044.1.v2.1
MHPFSRSQEAKEMFQAQSHLYNHTFNFISSMSLKCAVQLRIPDVIYDHKRPMTLIDLASKLRIHPSKATCLYRLMRVLVHSGIFAETVVPRDGKQEEEIKAYVLTPSSCLLLNDKATGLSPFVATMLDPALVSPWHFLGDWLGGNNHEDCRDKLNNPFEAVHGVNIWDYGRKNPEFGNTFNTAMAGDCQLMNLVIKDYKEIFEGLNSLVDVGGGTGTMARIISEVCPGLKCTLLDLPHVVAGLPESESENVKFVGGDMFESIPSADAILLKVCTLGIALL
>Cocit.E2043.1.v2.1
MHHLDHSSETKEMFQVQSHLYNLTSIFVSSMSLKCALQLGIPDVINEHKRPITLLELASALQIHPSKTTCLHRLMRVLVHLHIFAETLVPKHGGGGEEVEAYVLTPSSSLLLSDKATGLSPFIVAMLDPALMTPWHFLGDWFRGSHESNCSNKLISPFEAAHGLNLWDYGRKNPEFGHAFNGAMAIDSQMMNLIIRDCKHIFQGLNTLVDVGGGTGTVAKIISEACQGLKCTVLDFPHVVAGLPESENMKFVGGDMFEFIPPSDAILLKLMLHAWSDEDCVKVLRKCKEAIAGKNERGKVIILDIVIGETQDNDANAIATKLFFDMLMMTVVTGRERTEKEWEKLFLEAGFSQHKITPMFGLRSLIEVFP
>Cocit.F0095.1.v2.1
MFQAQSHLYNLTSVFVSSLSLKCAVQLGIPDVINDHKRPITLLEVASALQIHPSKTTCLDRLMRVLVHLHIFAETLVPKHEGEGEEVEAYVLTPSSILLLRDKTTGLSPFIVAMLDPALMTPWHFLGDWFRGSHESNCSNKLISPFEAAHGLNLWDYGRKNPEFGHAFNGAMAIDSQMMNLVIRDCKHIFQGLNTLVDVGGGTGTVAKLISEACQGLKCTVLDLPHVVAGLPESENVKFVGGDMFEFIPPSDAILLKLMLHAWNDEDCVKVLRKCREAIAGKNERGKVIIIDIVIGETQDDDAKAIATKLFFDMLMMTVVTGRERTEKEWEKLFSEAGFSQHKITPMFGLRSLIEVFP
>Cocit.F0094.1.v2.1
MSMKLYQRLLYRTRNFSLYYSNRVTPDSSSLVHSKAVHHLSHSHEAKEMFQAQSHLYNHTFNFISSMSLRCAVQLHIPDIIHDHKQPMTLLELASVLQIHPSKSTCLYRLMRVLVHSGIFTETLVLKNGEEEEEKTKAYVLTPSSRLLLSDKVVGVSPFVAAMLDPALVTPWHFLGDWLQGNNDIDCRDKSINPFMAAHGVDLWGYGRKNPEFGNTFNRAMASDSQMMNLVIQDCKEIFEGLN
>Cocit.H1843.1.v2.1
MNLGNGNGDATASELLRAQAHIWNHIFSFINSMCLKCAIDLGIPDLIHHHGRPMTLLELVSALHIQPTKAHCIHRLMRILIHSGFFQLRDLEEADGQTGYVLTRASCLLVKPNPSSVAPFVLLALTPFSMAPWQYLSAWLREGEDVHSSTFEMANGVPAWDAWRSPEVDGLFDEAMARDSSLMAKAVVDTCGGVFEGLSSVVDVGGGTGTMGKAIMEAFPHLECTVFDQPHVVDGLVGSERLKFVGGDMFVEIPSADAVFLKWILHDWSDEKCIEILKRSKEAVSGRGKVGKVIMIDMVVGNQTEDHRSTETQLFFDMLMMVNLNGKERSEKEWKKLFVDAGFRGYKIISRLGLRSLVEIYP
>Cocit.H1841.1.v2.1
MGKAIKEAFPHLECTVFDQPHVVDGLVGSERLKFVGGDMFVEIPPADVVFLKWILHDWSDEKCIEILKRSKEAVSGRGKVGKVIMIDMVVGNQTEDRRSTETQLFFDMMMANLNGKERSEKEWEKLFVDAGFSIYKIVSRLGLRSLVEIYP
>Cocit.K2190.1.v2.1
MQKAMSIKLNQRLLYRIGNFSLYYPNRVTPDSSSLAHSKTVHNLIHTHEAKKMFQAQSRLYNHTFNFISSMLLRCAVQLRIPDIIHDHKQPMTLPELASVLQIHPSKSTCLYRLMRVLVHLDIFTETLVLKNGEEEEEGGGKTKAYVLTPSSRLLLSDEVVGVSPFVAAMLDPALVTPWHFLGDWLQGSNVNDCRDKLINPFMAAHGVDIWGYGKKNPKFGNTFNRAMASDSQMMNLVIQDCKEIFEGLNSLVDVGGGMGIVARIISEACPGLKCTVLDLPQVVAGLPDSENVKFVGGDVFESIPSTDAILLKWMLHGWSDEDCVKVLRKCKEAIGDKNNDQGKVIIMDIVIDETEDNAQETATKLFFDMLMMTVATGRERTKKEWEKLFLEAGFKQHKITPMFGLKSLIEVFP
>Cocit.L5242.1.v2.1
MTMEEGVLDMNLGNGDGDATTSDLLRAQAHIWNHIFSFINSMCLKCAIDLGIPDLIHHHGRPMTLLELVSVLHIQPTKAHCIHRLMRILTHSGFFELQDLEEADRQTGYVLTCASRLLVKHNPLSAAPFALMALGPFSMGQWQYLSSWLRDAEDVHPSTFEMAYGLPMWEFGGRNPKVVSLFDEAMACDSSLMANVVVHTCGAVFEGLSLVVDVGGGTGTMAKAITEAFPHLECTVFDQPHVVDGLVGRERLKFVGGDMFVEIPPADAIFLKWILHNWSDEKCIEILKRSKEAVSNQGKVGKVIMIDMVVGNQIGDHRSTETQLFFDMLMMVNLNGKERSEKEWEKLFIDAGFSDYKIVSRLGLRSLIEIYP
>Cocit.L5244.1.v2.1
MHVVRGSRITEKGVFDMNLGNGDGGATASELLQAQAHIWNHTYSFINSVCLKWAIEQGIPDVIHHHGRPMTLLELVSALHVQPTKAHYIRRLMRILVHSGFFELRDLEEADHQTGYALTCVSRLLVKHNPSSMAPFALFALTPFPKGHWQYLSAWLRDGEDVHPSTFEMAYGVPTWDAWRSPEVNSLFNQAMASDSSLMAKVVVDTCGGVFEGLSSVVDVGGGTGTMGKAITAAFPHLECTVFDQPHVVDGLVGSERLKFVGGDMFVEIFPADAILLKWILHDWSDEKCIEILKRCREAVSSRGKVGKVIMIDMVVGNQIEDHRSVETQFFFDLLMMVTFNAKERDEKEWAKLFADAGFSGYKIVSCLGLRSLIEIYP
>Cocit.L3140.1.v2.1
MEEGVLDMNLGNGDGDATTSDLLRAQAHIWNHIFSFINSMCLKCAIDLGIPDLIHHHGRPMTLLELVSVLHIQPTKAHCIHRLMRILTHSGFFELQDLEEADRQTGYVLTCASRLLVKHNPLSVAPFALMALGPFSMGQWQYLSAWLRDAEDVHPSTFEMAYGLPMWEFGGRNPEVVGLFDEAMACDSSLMANVVVHTCGAVFEGLSLVVDVGGGTGTMAKAITDAFPHLECTVFDQPHVVDGLVGRERLKFVGGDMFVKIPPADAIFLKVSTMPLCCISNDIGSTWLQFKSTNRKFLFLRYT
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University