Enzyme
Isolated from the plants Perilla frutescens and Gentiana triflora (clustered gentian). Acts on a range of anthocyanidin 3-O-glucosides, 3,5-di-O-glucosides and cyanidin 3-rutinoside. It did not act on delphinidin 3,3',7-tri-O-glucoside. Recombinant Perilla frutescens enzyme could utilize caffeoyl-CoA but not malonyl-CoA as alternative acyl donor.
ANAT
Enzyme
Anthocyanin acyltransferase
Enzyme name
EC:2.3.1.215
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanin-3-O-glucoside 6''-O-acyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3-O-beta-D-glucoside = CoA + an anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [RN:R10237]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [CPD:C20461]
Product
Species information
Coptis chinensis
Species name
Coptis chinensis is a species of flowering plant in the family Ranunculaceae (buttercup family).
Species info
Gene sequence info
Coptis_chinensis.ANAT.fa
Download
>rna-gnl|WGS:JADFTS|Cch00036980-RA
MKHSLSLTLQHFFPFAGHLTWPQDSIKPEILYVDGDSVSFIVAESGYDFYTLVANHVKNDNEFYPLVPELLNMSNDNKLAPVLALQVTLFPNNGVCIGVTYNHAVADGGIVIQFMRSWASVCKLQGNTDSLIKSPLCYDRTMVKDPKGIEKIFLNSFSERNINKDTFNTAVPKIYIDPTDKVRSTFVITQTDIKRLKEWILSRLSTPPLHLSTFVVTCAYVWTCLVKATWGENGANASEDQIKYFGFSIDCRSRLDPPLPKTYFGTCICGEIVSAKQSDLVGLDGIAIAAELIGTVIKKMDNADEILESVEKMDSYDFPTMADHLLSVAGSAKLGAYETDFGWGIPKKTEVVSISATGAISLSECPNEVGAVEVGLVRNKIEMEAFISFFEKIIKGLA
>rna-gnl|WGS:JADFTS|Cch00036983-RA
MASRFNKTLKPEILYVDGDSVSFIVAESGYDFYTLVANYVKNDNEFYPLVPESLNMSNDNKLAPVLALQVTLFPNNGVCIGVTYNHAVADGGIVIQFMRSWASTDIKRLKEWILSRLSTPPLHLSTFVVTCAYVWTCLVKATWGENGANASEDQIKYFGFSIDCRSRLDPPLPKTYFGTCICGEIVSAKQSDLVGLDGIAIAAELIGTVIKKMDNADEILESVEKMDSYDFPTMADHLLSVAGSAKLGAYETDFGWGIPKKTEVVSISATGAISLSEYPNEVGAVESLRSLRPQLSPNERHQRQPAFAVCRTDKSEFIKTHELEISTLATLSSLEVLEAELEKLRKRRGELQKQHDNLKQIMSAAGYKQKVAEHRQNEDVVKLEKLMRELNIIEEAANNLERAIAGGR
>rna-gnl|WGS:JADFTS|Cch00010851-RA
MELPYTVDVLEQCQISPPFGSVPKTSLPLTFFDIIWLPLSLSPVQRLFMYEFNHPKSYFMDTILPNMKHSLSLTLQHFFPFAGNLTWPQDSTKPEILYNDGDSVSFTVAESDYDFHQLAGNHVKDANQLHPLVPDLLNMSNSKLASVLALQVTLSPHKGMCIGVTYNHTSADGGTVSQFIKSWASVCRLQGDTALIIESPPCYDRTIVKDPKGLEMILLDVTNNRYNINKETFNTPVPENSPDNLADKVRATFVLGQSDIKRLKQWILTRLSTPPLHLSTFVVTYSYVWVCLVKARWGQDVVANAYGDQKENILFPIDCRPKLDPPLPKTYFGNCLTAGIASAKRGDLIGLDGIAIAAELLGKAIKNVDSADKLMNNLETIILENKLQAMEGCLVSVAGSTKLGVYETDFGWGTPKKVEVVSIADTGAISLSECPNEIGAVEVGVVRNKIEMDAFSSLFVKTIQGFPQCNIDG
>rna-gnl|WGS:JADFTS|Cch00000667-RA
MTQVLALQVTLFPHYGLCIRFTIHHGAADGSTALQFMRSWSSLWKFTDELEKITVFAPFYDRSVIKDKDGLESIYLKDLRRFKWHLKTFKTSEITTNGVDMVRVTFILS
>rna-gnl|WGS:JADFTS|Cch00017053-RA
MVTENSSEGLQAAAITTLPYKVEVLETCQISPPLGSVSSTSLPLSFFDLTPWYTLFPIRAVYYFEVHHPKTYLINTIVPNIKHSLSLTLQYFFPLAGYISWPDDSTKPHILYKDGDSVPLTVAECNYDFDHLSGKHLRNNANELYPLIATFEEHALSNPKLIPLLNLQITLFPDSGICIGITYNHMAMDGRSANHFMKTWASLCRSQGDTSLVTESLPFHDRTMVKDRNGIELVYLKDLETMGMTKETCNILKIPDVPIDKAQGTFVISCSEIEKLRQWVSTRIQKDNQTIPAFHLSTFILISAYVWICSIKARRTTEDVLSLRKDPNEYFCFPIDCRARLDPPLPVTYFGNAVGTLVVNSDMNELIEENGMAIAAELIGKAIMNIDTEIWSTMAEGVSYCHSFPGEQLTYVSSSPKFAKNETDFGWGNKVKKIQTAGGSICLYDGPNEKGSVEIDLVRDAFEMNAFAALFTSSIENLRM
>rna-gnl|WGS:JADFTS|Cch00017050-RA
MEGPQGKIIYNRSSRGSKSKSLPALHLAGIVFKNIYPKYRATFINNRHGINNTLYAGTQLIMALTYKVEVLEICQISPPLGSISTASLPLTFFDIPWLVLPPVQTLFYYELNHPKTYFMNTILPNIKHSLSLTLQHFYPLAGQLTWPQDSVKPQLLYMDGDSVPLTVAECDYDFDHLSGNLSRNADEFYPLVPTFEHAMSDDKLVPILALQVTFFANKGICIGITFNHTSMDGQAFHHFIKSWASICKSEGITSLVTESLPFYDRTVVKDRKGIEIINLNDLEAMNITKDTYNILRIPMVPIDKVRGTFVMSYLDIKKLKQWVMTRLDDRHVAPSFRISTFILISSYVWICSIKARQDTQDDKSSCQDWREYFLFSIDCRPRLDPPLPMTYFGNCVGGCIANGDRNELIGENGMVVAAELIANAIKNVDTELWSRMEKGSSYYKSLPPGRFVSVGWSPKFGTYETDFGWGRPKKTEMNVSDGGAIALNSCRDEEGAIEVSLVRDAVEMDAFASLFENTVSALHL
>rna-gnl|WGS:JADFTS|Cch00017051-RA
MNTTVPTLKHSLSLTLQRFYPLAGHITWPHDSTKPHILYKDGDSVSLTVAECNYDFDHIAGNHLRNAGDLYPLVATFEEHALSDTKSVPISCFQITLFPNKGICIGFTFNHMAVDGRSADLIMKTWASLCRSQGDTSRVTESLPFYDRTMVKDRNGIELVNLKDLETMGMTKETCNILKTPKLPFNFVQGSFVMSCSEIGKLRQWVLSCLNKDKKFVPPFHLSTFTLISSYVWICSIKARRTTEDVLRSCEDQMEYFLFSADCRACLDPPLPVTYFGNCNGGFVVEGDRNELIGVNGIAIAAEMIGKAIMNLDTELWSVMGKGVSYYQRIPMAKLAIVSWSPKFAVYETDFGWGNPRRPKRLMRLIYVLLKVQMKKAPSRLT
>rna-gnl|WGS:JADFTS|Cch00032932-RA
MVTRSVSFIVAESGYDFYTLVANHVKNDNEFYSLVPELLNMSNDNKLAPVLALQVTLFLNNGICIGVTYNHAVADGGIVIQFMRSWASVCKLQSNTDSLIKSPLCYDRTMVKDPKGIEKIFLNSSKAWGENGESASEDHVKYFLFAMDCRSRLDPPLPKTYFGNCIWGGFVSAKQSDLVGLDGIAIAAELIGAVIKKMDNADEIMNMEKMDSYDFPTIADHLLSVAGSPKLGAYETDFGWGIPKKAEVVSISATVS
MKHSLSLTLQHFFPFAGHLTWPQDSIKPEILYVDGDSVSFIVAESGYDFYTLVANHVKNDNEFYPLVPELLNMSNDNKLAPVLALQVTLFPNNGVCIGVTYNHAVADGGIVIQFMRSWASVCKLQGNTDSLIKSPLCYDRTMVKDPKGIEKIFLNSFSERNINKDTFNTAVPKIYIDPTDKVRSTFVITQTDIKRLKEWILSRLSTPPLHLSTFVVTCAYVWTCLVKATWGENGANASEDQIKYFGFSIDCRSRLDPPLPKTYFGTCICGEIVSAKQSDLVGLDGIAIAAELIGTVIKKMDNADEILESVEKMDSYDFPTMADHLLSVAGSAKLGAYETDFGWGIPKKTEVVSISATGAISLSECPNEVGAVEVGLVRNKIEMEAFISFFEKIIKGLA
>rna-gnl|WGS:JADFTS|Cch00036983-RA
MASRFNKTLKPEILYVDGDSVSFIVAESGYDFYTLVANYVKNDNEFYPLVPESLNMSNDNKLAPVLALQVTLFPNNGVCIGVTYNHAVADGGIVIQFMRSWASTDIKRLKEWILSRLSTPPLHLSTFVVTCAYVWTCLVKATWGENGANASEDQIKYFGFSIDCRSRLDPPLPKTYFGTCICGEIVSAKQSDLVGLDGIAIAAELIGTVIKKMDNADEILESVEKMDSYDFPTMADHLLSVAGSAKLGAYETDFGWGIPKKTEVVSISATGAISLSEYPNEVGAVESLRSLRPQLSPNERHQRQPAFAVCRTDKSEFIKTHELEISTLATLSSLEVLEAELEKLRKRRGELQKQHDNLKQIMSAAGYKQKVAEHRQNEDVVKLEKLMRELNIIEEAANNLERAIAGGR
>rna-gnl|WGS:JADFTS|Cch00010851-RA
MELPYTVDVLEQCQISPPFGSVPKTSLPLTFFDIIWLPLSLSPVQRLFMYEFNHPKSYFMDTILPNMKHSLSLTLQHFFPFAGNLTWPQDSTKPEILYNDGDSVSFTVAESDYDFHQLAGNHVKDANQLHPLVPDLLNMSNSKLASVLALQVTLSPHKGMCIGVTYNHTSADGGTVSQFIKSWASVCRLQGDTALIIESPPCYDRTIVKDPKGLEMILLDVTNNRYNINKETFNTPVPENSPDNLADKVRATFVLGQSDIKRLKQWILTRLSTPPLHLSTFVVTYSYVWVCLVKARWGQDVVANAYGDQKENILFPIDCRPKLDPPLPKTYFGNCLTAGIASAKRGDLIGLDGIAIAAELLGKAIKNVDSADKLMNNLETIILENKLQAMEGCLVSVAGSTKLGVYETDFGWGTPKKVEVVSIADTGAISLSECPNEIGAVEVGVVRNKIEMDAFSSLFVKTIQGFPQCNIDG
>rna-gnl|WGS:JADFTS|Cch00000667-RA
MTQVLALQVTLFPHYGLCIRFTIHHGAADGSTALQFMRSWSSLWKFTDELEKITVFAPFYDRSVIKDKDGLESIYLKDLRRFKWHLKTFKTSEITTNGVDMVRVTFILS
>rna-gnl|WGS:JADFTS|Cch00017053-RA
MVTENSSEGLQAAAITTLPYKVEVLETCQISPPLGSVSSTSLPLSFFDLTPWYTLFPIRAVYYFEVHHPKTYLINTIVPNIKHSLSLTLQYFFPLAGYISWPDDSTKPHILYKDGDSVPLTVAECNYDFDHLSGKHLRNNANELYPLIATFEEHALSNPKLIPLLNLQITLFPDSGICIGITYNHMAMDGRSANHFMKTWASLCRSQGDTSLVTESLPFHDRTMVKDRNGIELVYLKDLETMGMTKETCNILKIPDVPIDKAQGTFVISCSEIEKLRQWVSTRIQKDNQTIPAFHLSTFILISAYVWICSIKARRTTEDVLSLRKDPNEYFCFPIDCRARLDPPLPVTYFGNAVGTLVVNSDMNELIEENGMAIAAELIGKAIMNIDTEIWSTMAEGVSYCHSFPGEQLTYVSSSPKFAKNETDFGWGNKVKKIQTAGGSICLYDGPNEKGSVEIDLVRDAFEMNAFAALFTSSIENLRM
>rna-gnl|WGS:JADFTS|Cch00017050-RA
MEGPQGKIIYNRSSRGSKSKSLPALHLAGIVFKNIYPKYRATFINNRHGINNTLYAGTQLIMALTYKVEVLEICQISPPLGSISTASLPLTFFDIPWLVLPPVQTLFYYELNHPKTYFMNTILPNIKHSLSLTLQHFYPLAGQLTWPQDSVKPQLLYMDGDSVPLTVAECDYDFDHLSGNLSRNADEFYPLVPTFEHAMSDDKLVPILALQVTFFANKGICIGITFNHTSMDGQAFHHFIKSWASICKSEGITSLVTESLPFYDRTVVKDRKGIEIINLNDLEAMNITKDTYNILRIPMVPIDKVRGTFVMSYLDIKKLKQWVMTRLDDRHVAPSFRISTFILISSYVWICSIKARQDTQDDKSSCQDWREYFLFSIDCRPRLDPPLPMTYFGNCVGGCIANGDRNELIGENGMVVAAELIANAIKNVDTELWSRMEKGSSYYKSLPPGRFVSVGWSPKFGTYETDFGWGRPKKTEMNVSDGGAIALNSCRDEEGAIEVSLVRDAVEMDAFASLFENTVSALHL
>rna-gnl|WGS:JADFTS|Cch00017051-RA
MNTTVPTLKHSLSLTLQRFYPLAGHITWPHDSTKPHILYKDGDSVSLTVAECNYDFDHIAGNHLRNAGDLYPLVATFEEHALSDTKSVPISCFQITLFPNKGICIGFTFNHMAVDGRSADLIMKTWASLCRSQGDTSRVTESLPFYDRTMVKDRNGIELVNLKDLETMGMTKETCNILKTPKLPFNFVQGSFVMSCSEIGKLRQWVLSCLNKDKKFVPPFHLSTFTLISSYVWICSIKARRTTEDVLRSCEDQMEYFLFSADCRACLDPPLPVTYFGNCNGGFVVEGDRNELIGVNGIAIAAEMIGKAIMNLDTELWSVMGKGVSYYQRIPMAKLAIVSWSPKFAVYETDFGWGNPRRPKRLMRLIYVLLKVQMKKAPSRLT
>rna-gnl|WGS:JADFTS|Cch00032932-RA
MVTRSVSFIVAESGYDFYTLVANHVKNDNEFYSLVPELLNMSNDNKLAPVLALQVTLFLNNGICIGVTYNHAVADGGIVIQFMRSWASVCKLQSNTDSLIKSPLCYDRTMVKDPKGIEKIFLNSSKAWGENGESASEDHVKYFLFAMDCRSRLDPPLPKTYFGNCIWGGFVSAKQSDLVGLDGIAIAAELIGAVIKKMDNADEIMNMEKMDSYDFPTIADHLLSVAGSPKLGAYETDFGWGIPKKAEVVSISATVS
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University