Enzyme
One of three specific glucosyltransferases in pea (Pisum sativum) that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase). TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly.
UGT79B30
Enzyme
UDP-glycosyltransferase 79B30
Enzyme name
EC:2.4.1.239
Entry
Transferases;Glycosyltransferases;Hexosyltransferases
Class
UDP-glucose:flavonol-3-O-beta-D-glucoside 2''-O-beta-D-glucosyltransferase
Sysname
UDP-glucose + a flavonol 3-O-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside [RN:R07339]
Reaction
UDP [CPD:C00015];flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside [CPD:C15581]
Product
Species information
Coptis chinensis
Species name
Coptis chinensis is a species of flowering plant in the family Ranunculaceae (buttercup family).
Species info
Gene sequence info
Coptis_chinensis.UGT79B30.fa
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>rna-gnl|WGS:JADFTS|Cch00020981-RA
MSSSQRSSNLALDVAMFPFLAGGHQIPFLQLSNKLAQRGHRVSFLLTNKTKLKFEPYNLHPNLITFIPLTVPHTDGLPLGTETMADVSRSMAPLFLKVIDKMSDQVEAVLRDLKPNIVFYDFFHWIPSLARPLGAKPILYTILDPVAFAYLFLPVRELQLPKGENLDETFLLQPPPCFPDSSIYLHLHEARELLEYYKEFISGMTTLEFVRKCVKECDAWCFRTCREID
>rna-gnl|WGS:JADFTS|Cch00020983-RA
MSSSQRSSNLVLHVAMFPFLAAGHQIPFLQLSNKLAQRGHRVSFLLTNKTKLKFEPYNLYPNLITLIPLSVPHIDGVPLGTETLADVSRSMAPLFLKVIDKMSDQVEAVLRDLKPNIVFYDLFHWIPSLARPVGVKSIFYSILDLLALAYLFLPVRELQLPKGKNLDEALLLQPPPGFPDSSIYLHLHEARCSLEYYKEFISGMTTLELLRKRVKESDAWCLRTCREIDGAFSDCLETHYEKQIVLAGPVIDFPPASSTLEERWTNWLDGFKTGSVVYCAFGSECVLEKDKFQELLLGFEMTGLPFLVALKPPFGAETVEETLPEGFQERVEGRGVVYGGWVQQQLILRHPSVGCFISHCGSSSIWEALASECQLVLLPQGSELFVCKKD
>rna-gnl|WGS:JADFTS|Cch00034499-RA
MSSSQRSSNLALHVAMFPFLAGGHQIPFLQLSNKLAQRGHRVSFLLTNKTKLKFEPYNLYPNLITFIPLTVPHTDGLPLGTETMADVSRSMAPLFLKVIDKMSDQVDAVLRDLKPNIVFYDFFHWIPSLARPLGAIPIFYTILDPVAFAYLFLPVRELQLPKGENLDETLLLQPPPGFPDSSIYLHLHEARESLEFYKEFISGMTTVEFLRKCVKECDAWCFRTCREIDGAFSDCLETHYEKQIVLAGPVIDFPPASSTLEERWTNWLDGFKTGSVVYCAFGSECVLEKDKFQELLLGFEMTGLPFLVALKPPFGAETVEEALPEGFQERVEGRGVVYGGWVQQQLILRHPSVGCFISHCGSSSLWEALVNECQLVLLPQGTELFVSARRISTYLKAGVEIERREEDGWFNRESVFKAIKMVMDEDSKVGTEIRANKTKWKDFLSQESLDSSYIDNLVLKLQDLVD
>rna-gnl|WGS:JADFTS|Cch00020982-RA
MIGSVVYYAFGSECVLEKDKFQELLLGFELTGLPFLVALKPPFGAKIRDLSPARSHYGSSSLWEALLNECLIVLLPQGTELFVSARRISTYLKAGVEIERREEDRWFNPESVFKAIKMVMDEDNKVGTEIKANKTKWKDFLSQESLDSSYIDNLVLKLQDLVD
>rna-gnl|WGS:JADFTS|Cch00006683-RA
MSSSQRSSNLLHVAMFPFLAAGHQIAFLQLSNKLAQRGHRVSFLLTNKTKLKFESYNLYPNLITFIPLAVPHIDGLPLGTETMADVSRSMAPLFLNVIDKMSIQVEPVLRDLKPNIVFYDFFHWIPSLARPLGVKSILYSTLDALTVAYLFLPIRELQLPKGEDLDETQLLQPPPGFPDSSIYLHLHEARGSLDYYKDFASGMTTLEFARKCLKECDAWCFRTCREIDGAFSDCLETHYKKQIVLAGPVIDFPPSSSILEERWISWLDGFKTGSVVYCAFGSECVLEKDKLQELLLGFEMTGLPFLVALKPPFGAESVEEALPEGFQERVKGRGMVYGGWVQQQLILRHPSVGCFISHCGAASIWEALVNDCQLVLLPQGGEQFLSARRISTYLNAGVEIERREEDGWFNRESVLKAIKMVMDEDSKVGTEFSEKSDVFS
>rna-gnl|WGS:JADFTS|Cch00020471-RA
MARVIQACQAVAIRSCKEYEGDYLDVAKKPMWADDEDDALQAGFSSRTSNRGFVLLGWAPYLYIFAHPSIGGSLFHSGMGLNHRDTPAWSHPSGAAFSG
>rna-gnl|WGS:JADFTS|Cch00020477-RA
MVTTPTFHIAMFPWFAMGHITQYLHFSNKFAERGHRITFFIPTKTQHKFSQLNHKSDLIKFISIDIPPVDGLAVGVETMSDVPLHKGSLFAQAFDPTQDQVKVVLQHLKPNFIFFDLAYWVPTLACPLGIKPMYFPTGCRELEGHFCEYIGNQYKKRVLLLGPVLRKPSHIPLAEKWGKWLAGFEKGSVVYCAFGSECVLTKDQFQELVLGLELTGFPFIVRLKPPFGVDTVEEALPDGFKDRIGGRGVVHGDWVQQELILNHPSVGCFVSHCGFGSMWESLVNTCQIIVVPHAGDQFVNAKFMTRELKVAVDVERREEDGWFTKESMCKAVKLVMDQGSEVGEQVRANHHKWKDFLLSEGLESSYLDNFFIKLQDTLN
>rna-gnl|WGS:JADFTS|Cch00006735-RA
MSDVPMQKASLLAVVFDILKGQVEIALHKLKPESVFFDFAYWLPGLAVPLGIKCIFFSLISGVSSAYFLLPSRKLEKGQHFTELDLIEPPPERRKEDGWFTRESVWKAVKLAMDEGSEVGGEIRANHPEMERFLVN
>rna-gnl|WGS:JADFTS|Cch00007594-RA
MVTTPTFHIAMFPWFAMGHITQYLHFSNKFAERVDGLAVGVETMSDVPLHKGSLFAQAFDLTQDQVKVVLQHLKPNFIFFDLAYWVPTLACPLGIKPMYFPTVSVATLTMLVSSMNLKYNIGTTRFSK
>rna-gnl|WGS:JADFTS|Cch00004473-RA
MSDVPLHIASLFAQAFNLTQDQLKVSLQNLRPDFIFFDLAYWDPSIARPLGIKLIYFSTVSVSSHSMINFPMNVNYKIDPPPDYPSSIFSMKMPLYIARHILHMSCYFGNGISFLERLITSIFGCQALCFHGCRELEGPFSDFMEKQYKKPISLIGPVLPKPSCRPLDEKWVKWLGGFKKGSVVYCAFGSECVLEKDQFQELVLGLELTGLPFIVRLKPPVGVSTVEEALPKGFEERTRGKRSSSWRLGSARTCSKSPFSRMFCESLWLWVNVGLLGEYLSSSFGAPCWRPIFQH
>rna-gnl|WGS:JADFTS|Cch00028608-RA
MSTQPTYHVAMFPWFAIGHITPFLHVSNKLDERGHRVTFLIPKNTQNKFNHLNLHPHLIQFIPLTVPKVDGLPPGAETMSDVPIQKASLLAVAFDILKGQVEIALHKLKPDAVFFDFAYWLPALAVPLGIKCIFFSIISAVSSAYFLVPSRKLEKGQHFTELDLIEPPPGYPSASPFNYLPFELRDAKDFFSTEFHGGISFFHRIITSMTESLAHCFITCSELEGPFCDYIENQYNKPILLTGPILPDPSSNSPLEEKWAKWLGGFKEGSVVYCAFGSECVLQKDQFQELVLGLEHTGLPFLVRLKPPFGCETLVEALPEGFEERVRGRGVVHGDWVQQQLILKHPSVGCFVLWAWVYVGIFDEHLPNSFGTPPWGSVCKYQIRD
>rna-gnl|WGS:JADFTS|Cch00040053-RA
MYPWFAMGHLTAFMHLANKLAQRGHKVSFILPPKAESKLSHFNLFPELVTFIPITFPQVDGLPLGAETTSDVPLVQCSLIMTAMDRTKTQIESKLSELRPHFVFFDFTHWLPELTQKLGIKSVHYCIISSVSVAYLLVPVRQQENTQNQLRLMEKPMGFPSNSIKLKKFEASRAQFFKGMEFGSGITFHDRNIQSLKNADALGFRTCREMEGTFCDYVKTQFGGKPVLLTGPILPSQPTDTLEPRWVDWLSKFSPSSVVYVAFGSECTLKMEQFQELLLGLEMTGSPFLVALKVPEGVEKIEDALPYGFEERVKERGIVYGGWIQQQLLLGHPSMGCFVTHCGSGSISESLVSECQIVLLPQYGDQCINARMMGGEMKVGVEVEKREEDGWYTKESVSNAVKLVMDEDSQIGKEVRSHHAKWRAFLLSEGLESSYIENFIKSLQSILG
>rna-gnl|WGS:JADFTS|Cch00017678-RA
MDQSTLHIVMYPWFAFGHMNSFLNLANKFALRGHRISFFTPTKAQAKLEPSNAYPNLIRFIPIVVPHVEGLPVGAETTADVPDDKGSLIMTAMDRTRPDVEVALKELKPDLVFFDFTYWLPSVTRPLGIKSVQYCITSTATVAYILVPASSRLDKGLQLSEEELMNPPPDFPPNSSIKLHRHEARSLSHTTYRQYGDNISFHERLVYSLRDADAFGFRSCKEIEGPYCDYVGNHFGKPTLLLGLTLPTPPSTQLEEKWAKWLNQCKPGSVIFCAFGSECVMEKNQFQELLLGFELTGLPFFVALKPPTGTNTVDEALPEGFKERVQGRGIVEGGWVQQQHIMAHPSVGCFVGHSGLSSVTEALINNPQLILIPQRGDQYIIVRLMSGDLKVAVEIEAREEDGWYTKENVCNAVNTVMDENNETGKQVRANRLKLKEFLLRDDLEASCIDTSLEKMKAMVKK
MSSSQRSSNLALDVAMFPFLAGGHQIPFLQLSNKLAQRGHRVSFLLTNKTKLKFEPYNLHPNLITFIPLTVPHTDGLPLGTETMADVSRSMAPLFLKVIDKMSDQVEAVLRDLKPNIVFYDFFHWIPSLARPLGAKPILYTILDPVAFAYLFLPVRELQLPKGENLDETFLLQPPPCFPDSSIYLHLHEARELLEYYKEFISGMTTLEFVRKCVKECDAWCFRTCREID
>rna-gnl|WGS:JADFTS|Cch00020983-RA
MSSSQRSSNLVLHVAMFPFLAAGHQIPFLQLSNKLAQRGHRVSFLLTNKTKLKFEPYNLYPNLITLIPLSVPHIDGVPLGTETLADVSRSMAPLFLKVIDKMSDQVEAVLRDLKPNIVFYDLFHWIPSLARPVGVKSIFYSILDLLALAYLFLPVRELQLPKGKNLDEALLLQPPPGFPDSSIYLHLHEARCSLEYYKEFISGMTTLELLRKRVKESDAWCLRTCREIDGAFSDCLETHYEKQIVLAGPVIDFPPASSTLEERWTNWLDGFKTGSVVYCAFGSECVLEKDKFQELLLGFEMTGLPFLVALKPPFGAETVEETLPEGFQERVEGRGVVYGGWVQQQLILRHPSVGCFISHCGSSSIWEALASECQLVLLPQGSELFVCKKD
>rna-gnl|WGS:JADFTS|Cch00034499-RA
MSSSQRSSNLALHVAMFPFLAGGHQIPFLQLSNKLAQRGHRVSFLLTNKTKLKFEPYNLYPNLITFIPLTVPHTDGLPLGTETMADVSRSMAPLFLKVIDKMSDQVDAVLRDLKPNIVFYDFFHWIPSLARPLGAIPIFYTILDPVAFAYLFLPVRELQLPKGENLDETLLLQPPPGFPDSSIYLHLHEARESLEFYKEFISGMTTVEFLRKCVKECDAWCFRTCREIDGAFSDCLETHYEKQIVLAGPVIDFPPASSTLEERWTNWLDGFKTGSVVYCAFGSECVLEKDKFQELLLGFEMTGLPFLVALKPPFGAETVEEALPEGFQERVEGRGVVYGGWVQQQLILRHPSVGCFISHCGSSSLWEALVNECQLVLLPQGTELFVSARRISTYLKAGVEIERREEDGWFNRESVFKAIKMVMDEDSKVGTEIRANKTKWKDFLSQESLDSSYIDNLVLKLQDLVD
>rna-gnl|WGS:JADFTS|Cch00020982-RA
MIGSVVYYAFGSECVLEKDKFQELLLGFELTGLPFLVALKPPFGAKIRDLSPARSHYGSSSLWEALLNECLIVLLPQGTELFVSARRISTYLKAGVEIERREEDRWFNPESVFKAIKMVMDEDNKVGTEIKANKTKWKDFLSQESLDSSYIDNLVLKLQDLVD
>rna-gnl|WGS:JADFTS|Cch00006683-RA
MSSSQRSSNLLHVAMFPFLAAGHQIAFLQLSNKLAQRGHRVSFLLTNKTKLKFESYNLYPNLITFIPLAVPHIDGLPLGTETMADVSRSMAPLFLNVIDKMSIQVEPVLRDLKPNIVFYDFFHWIPSLARPLGVKSILYSTLDALTVAYLFLPIRELQLPKGEDLDETQLLQPPPGFPDSSIYLHLHEARGSLDYYKDFASGMTTLEFARKCLKECDAWCFRTCREIDGAFSDCLETHYKKQIVLAGPVIDFPPSSSILEERWISWLDGFKTGSVVYCAFGSECVLEKDKLQELLLGFEMTGLPFLVALKPPFGAESVEEALPEGFQERVKGRGMVYGGWVQQQLILRHPSVGCFISHCGAASIWEALVNDCQLVLLPQGGEQFLSARRISTYLNAGVEIERREEDGWFNRESVLKAIKMVMDEDSKVGTEFSEKSDVFS
>rna-gnl|WGS:JADFTS|Cch00020471-RA
MARVIQACQAVAIRSCKEYEGDYLDVAKKPMWADDEDDALQAGFSSRTSNRGFVLLGWAPYLYIFAHPSIGGSLFHSGMGLNHRDTPAWSHPSGAAFSG
>rna-gnl|WGS:JADFTS|Cch00020477-RA
MVTTPTFHIAMFPWFAMGHITQYLHFSNKFAERGHRITFFIPTKTQHKFSQLNHKSDLIKFISIDIPPVDGLAVGVETMSDVPLHKGSLFAQAFDPTQDQVKVVLQHLKPNFIFFDLAYWVPTLACPLGIKPMYFPTGCRELEGHFCEYIGNQYKKRVLLLGPVLRKPSHIPLAEKWGKWLAGFEKGSVVYCAFGSECVLTKDQFQELVLGLELTGFPFIVRLKPPFGVDTVEEALPDGFKDRIGGRGVVHGDWVQQELILNHPSVGCFVSHCGFGSMWESLVNTCQIIVVPHAGDQFVNAKFMTRELKVAVDVERREEDGWFTKESMCKAVKLVMDQGSEVGEQVRANHHKWKDFLLSEGLESSYLDNFFIKLQDTLN
>rna-gnl|WGS:JADFTS|Cch00006735-RA
MSDVPMQKASLLAVVFDILKGQVEIALHKLKPESVFFDFAYWLPGLAVPLGIKCIFFSLISGVSSAYFLLPSRKLEKGQHFTELDLIEPPPERRKEDGWFTRESVWKAVKLAMDEGSEVGGEIRANHPEMERFLVN
>rna-gnl|WGS:JADFTS|Cch00007594-RA
MVTTPTFHIAMFPWFAMGHITQYLHFSNKFAERVDGLAVGVETMSDVPLHKGSLFAQAFDLTQDQVKVVLQHLKPNFIFFDLAYWVPTLACPLGIKPMYFPTVSVATLTMLVSSMNLKYNIGTTRFSK
>rna-gnl|WGS:JADFTS|Cch00004473-RA
MSDVPLHIASLFAQAFNLTQDQLKVSLQNLRPDFIFFDLAYWDPSIARPLGIKLIYFSTVSVSSHSMINFPMNVNYKIDPPPDYPSSIFSMKMPLYIARHILHMSCYFGNGISFLERLITSIFGCQALCFHGCRELEGPFSDFMEKQYKKPISLIGPVLPKPSCRPLDEKWVKWLGGFKKGSVVYCAFGSECVLEKDQFQELVLGLELTGLPFIVRLKPPVGVSTVEEALPKGFEERTRGKRSSSWRLGSARTCSKSPFSRMFCESLWLWVNVGLLGEYLSSSFGAPCWRPIFQH
>rna-gnl|WGS:JADFTS|Cch00028608-RA
MSTQPTYHVAMFPWFAIGHITPFLHVSNKLDERGHRVTFLIPKNTQNKFNHLNLHPHLIQFIPLTVPKVDGLPPGAETMSDVPIQKASLLAVAFDILKGQVEIALHKLKPDAVFFDFAYWLPALAVPLGIKCIFFSIISAVSSAYFLVPSRKLEKGQHFTELDLIEPPPGYPSASPFNYLPFELRDAKDFFSTEFHGGISFFHRIITSMTESLAHCFITCSELEGPFCDYIENQYNKPILLTGPILPDPSSNSPLEEKWAKWLGGFKEGSVVYCAFGSECVLQKDQFQELVLGLEHTGLPFLVRLKPPFGCETLVEALPEGFEERVRGRGVVHGDWVQQQLILKHPSVGCFVLWAWVYVGIFDEHLPNSFGTPPWGSVCKYQIRD
>rna-gnl|WGS:JADFTS|Cch00040053-RA
MYPWFAMGHLTAFMHLANKLAQRGHKVSFILPPKAESKLSHFNLFPELVTFIPITFPQVDGLPLGAETTSDVPLVQCSLIMTAMDRTKTQIESKLSELRPHFVFFDFTHWLPELTQKLGIKSVHYCIISSVSVAYLLVPVRQQENTQNQLRLMEKPMGFPSNSIKLKKFEASRAQFFKGMEFGSGITFHDRNIQSLKNADALGFRTCREMEGTFCDYVKTQFGGKPVLLTGPILPSQPTDTLEPRWVDWLSKFSPSSVVYVAFGSECTLKMEQFQELLLGLEMTGSPFLVALKVPEGVEKIEDALPYGFEERVKERGIVYGGWIQQQLLLGHPSMGCFVTHCGSGSISESLVSECQIVLLPQYGDQCINARMMGGEMKVGVEVEKREEDGWYTKESVSNAVKLVMDEDSQIGKEVRSHHAKWRAFLLSEGLESSYIENFIKSLQSILG
>rna-gnl|WGS:JADFTS|Cch00017678-RA
MDQSTLHIVMYPWFAFGHMNSFLNLANKFALRGHRISFFTPTKAQAKLEPSNAYPNLIRFIPIVVPHVEGLPVGAETTADVPDDKGSLIMTAMDRTRPDVEVALKELKPDLVFFDFTYWLPSVTRPLGIKSVQYCITSTATVAYILVPASSRLDKGLQLSEEELMNPPPDFPPNSSIKLHRHEARSLSHTTYRQYGDNISFHERLVYSLRDADAFGFRSCKEIEGPYCDYVGNHFGKPTLLLGLTLPTPPSTQLEEKWAKWLNQCKPGSVIFCAFGSECVMEKNQFQELLLGFELTGLPFFVALKPPTGTNTVDEALPEGFKERVQGRGIVEGGWVQQQHIMAHPSVGCFVGHSGLSSVTEALINNPQLILIPQRGDQYIIVRLMSGDLKVAVEIEAREEDGWYTKENVCNAVNTVMDENNETGKQVRANRLKLKEFLLRDDLEASCIDTSLEKMKAMVKK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University