Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Coix lacryma-jobi
Species name
Job's tears (Coix lacryma-jobi) is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Coix_lacryma-jobi.4CL.fa
Download
>Adlay0222_T0270
MGSVAEDSAAAAAAASVVFRSKLPDIEIPRHLSLQAYCFERLPSVSSRPCLIDGQTGAVHTYADVDLLSRRAAAALRGLGVGKGDVVMNLLRNCPEFAFVFLGAARLGAATTTANPFYTPHEIHRQAAAAGARVVVTEACAVEKVRAFAAERGVPVVAVDGAFEGCLELGALMRAAEPLAADEDVDPDDVVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGENPNLYFSSDDVVLCVLPLFHIYSLNSVLLAGLRAGCSIVIMRKFEIGALVELVRAHGVTVAPFVPPIVVEIAKSPRVGADDLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFAKEPFQVKSGSCGTVVRNAELKIVDPDTSAFLGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDELAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILRKDLRARLAAGVPTSDNTQSKS
>Adlay0256_T0190
MVSPTEPQAETTVFRSTLPDIAIPDHLPLHDYVLERLAERRDRACLIDGATGETLTFGDVDRLSRRVAAGMRATLGVRSGGTVMLLLPNSVEFALAFLACSRLGAAATTANPLHTPPEIAKQAAASGATVVITEPAFVGKVRGLAGVAVVATGDGAEGCVSFSDLASTDGSALPEAAIDVANDVVALPYSSGTTGLPKGVMLSHRGLVTSVAQLVDGDNPNLHIREDDVVLCVLPMFHVYSLHSILLCGMRAGAALVIMKRFDTLRMFELVKRHGVTVVPLVLPIAVEMAKSDAVDRHDLSSVRMVISGAAPMGKELQDMLRARLPRAVLGQGYGMTEAGPVLSMCMAFAKEPSPVKSGACGTVVRNAELKIVDPETGLSLHRNQPGEICIRGKQLMKGYLNNPEATAKTIDSEGWLHTGDIGYVDDDDEIFIVDRLKELIKYRGFQVAPAELEAMLIAHHSIADAAVVPMKDDYSGEIPVAFVVTSGGSEITEDEIKQYVAKQVVFYKRLHKIFFVEAIPKAPSGKILRKDLRAKLASGFSNGSSC
>Adlay0318_T0070
MGSIDAVVAVPVPAAEPSAAEEKAVVFRSKLPDIEINNSQSLQAYCFGKMGEVADRACLIDGQTGASYTYAEVESLSRRAASGLRAMGVGRGDVVMNLLRNCPEFAFAFLGAARLGAATTTANPFYTPHEIHRQAEAAGARLIVTEACAVEKVREFAAARGVPVVTVDGRFDGCVEFAELIAAEELEADADVHPDDVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGENPNLYFSKDDVLLCLLPLFHIYSLNSVLLAGLRAGSTMVIMRKFDLGALVELVRKYVITIAPFVPPIVVEIAKSPRVTADDLASIRMVMSGAAPMGKELQDAFMAKIPNAMLGQGYGMTEAGPVLSMCLAFAKEPFQVKSGSCGTVVRNAELRIVDPDTGAALGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDDLAGEIPVAFIVRTEGSEVTEDEIKKFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARLAAGVH
>Adlay1127_T0300
MRMFVFLWWINCRRRGMATPSLPSQIVEFNYKTMEDKLKRLALKVSHHEDNIRFLKSQLNAVEEACVDLGIKLGNYHASVAAVVNNDTSAQEAEERTIRTILDQDKTAAGIICQLKVRHHELASKMPLMKDILGFVATLGKVNNDNLSRLLTEYLGMDNMLALVCKTYDGVKGLEKYDKDGIIDKTSGVHGLGRSVGKFLDGRFTVFCLENLSKNLEVKFPVNIEVSSLPENIAEMEEQVKLKNWEKERVLEDMKREGDLLKQVKELYMKQKQEFMDYLAQPAVAQALHDSPTTHSPATPGSNPFGAKPSQKRWLFLLHGLPWKSSLRGQCELNSKDMSMTQPNLTCTTPRKNCVNHMPSPSSRLHQSLPTMPRPPPPLVSSNANAAPFLQLPTKQQQQQLRRRMGDAAIVTTGVHLHEAAEEEQEHIFRSRFPPVAVPDDVTVPEFVLAGAEAYADKTALVEAAPGGRSYTYGEVARDVARFARALRSVGVRRGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRAAAEEIRKQVEDSEARLVVADAVAYDKVKDIIGVPVIGIGGGLLPGAIGWDELLAAADRTGAPVPVVALDAARQSDLCALPYSSGTTGVSKGVMLSHRNLVSSLCSSMFAVGEELVGQVVTLGLMPFFHIYGITGICCATLRHKGTVVVMDRFDLRAFLGALLAHRVMFAPIVPPVMLAMVKSPVADEMDLSGLALRSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGGGEEGGPVQVAKKGSVGFVLPNLEVKFVDPGTGRSLPKNTPGEICVRSQAVMRGYYGRKDETERTVDAAGWLHTGDVGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLSHPSVQDAAVFGLPDEEAGEVPASCVVRRRGAAESEADVMAYVAGRVASYKKLRLLRFVDAIPKSVSGKILRRQLRDDFIINRAAQAQDGGGASTCQVQS
>Adlay1326_T0120
MARTAAPAAGYGADGVYRSPRPAVRIASDPGLSLNDLLFRRADACPSALALVDSATGQSLTFAAFRSAVLTTAVALSSRAGVRRGDVVLFFAPNCFLYPVCFFAVTALGAVATTANPLYTPREVAKQATDSRAKLAITVSELLPKIVGLGLPTILLDGDGDGGAASATAASRPQGVSVTLYSDLISGARETEYRRPPTKQSDTAALLYSSGTTGASKGVVLTHRNFISAAAMVTADQDAHGEGPNVFLCFLPMFHIFGLSVITFAQMQRGNSVVVMSRFDMDSVMAAVQRHRITHLFCVPPVMIALAKLGSVGKYDLSSLRFIGSGAAPLGKDVMEGVANNFPEAVIAQGYGMTETCGIISLEYPEEGQVRQFGSTGALVSGVEAKIVDVETLKCLPPNQLGEICVRGLNIMQGYFNNVQATEFTIKQGWLHTGDIGYFDDGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDAEAGEVPIAYVVRSPVSSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRELIAKVRSSKL
>Adlay1397_T0010
MGSTEEQQQQAAVEEASPEIIFRSKLPDIAISNTLPLHRYCFELLPEVGERPCLIDGATDAVLTYGEVDRLSRRLAAALRRAPLGLERGAVVMNLMLNSAEFVLSFFAASRVGAAVTTANPMSTPHDIAKQIAASGATVVFTESMAVDKLPGGVTVVLIDAHRDGCLHFWDDVIASVPDDEDQEEEAAEAAAGFDPDDVVALPYSSGTTGLPKGVMLTHRSLSTSVAQQVDGDNPNIGFTAADVILCSLPMFHIYSLNTIMMCGLRVGAAIVVMRRFDLARMMQLVERHRITIAPLVPPIVVAVAKSDEAAAHDLSSVRMVLSGAAPMGKDIEDAFMAKLPAAVLGQGYGMTEAGPVLSMCLAFAKEPFKVKSGACGTVVRNAELKIVDPDTGKSLPRNLPGEICIRGQQIMKGYLNNPEATKNSIDADGWLHTGDVGFVDDDDEIFIVDRLKEIIKYKGLQVAPAELEALLITHPSIADAAVVGKQVEPEIGEIPVAFVAKAKGSELNEDDVKQFVAKEVIYYKKVREVIFVDKIPKAPSGKILRKELRKQLQQQQQQLA
>Adlay1868_T0090
MITVAAPEAQPQAAAAPAAPEETVFRSKLPDIDIPSHLPLHDYCFARAAEVADAPCLIAAATGRTYTYAETRLLCRKAAASLHGLGVGQGDRVMVLLQNSVEFVLTFLGASFLGAVTTAANPFCTPLEIHKQLRASGAKLVVTQSAYVDKLRHEAFPRIGAASGGDGNALTVLTTDDDDAAGTGTPPEGCLAFWELVAPADDAALPEVSISPDDPVALPFSSGTTGLPKGVVLTHGGQVSNVAQQVDGANPNLYMREGDVALCVLPLFHIFSLNSVLLCALRAGAAVMLMPRFEMGAMLEGIQRWCVTVAAVVPPLVLALAKNPALEKYDLSSIRIVLSGAAPLGKELVDALRARVPQAVFGQGYGMTEAGPVLSMCPAFAKEPTPAKPGSCGTVVRNAELKVVDPDTGLSLGRNLPGEICIRGPQIMKGYLNDPEATARTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLIAHPSIADAAVVPQKDDAAGEVPVAFVVRAADSDIAEDAIKEYISKQVVFYKRLHKVYFALSIPKSASGKILRRELRAKLAAAATT
MGSVAEDSAAAAAAASVVFRSKLPDIEIPRHLSLQAYCFERLPSVSSRPCLIDGQTGAVHTYADVDLLSRRAAAALRGLGVGKGDVVMNLLRNCPEFAFVFLGAARLGAATTTANPFYTPHEIHRQAAAAGARVVVTEACAVEKVRAFAAERGVPVVAVDGAFEGCLELGALMRAAEPLAADEDVDPDDVVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGENPNLYFSSDDVVLCVLPLFHIYSLNSVLLAGLRAGCSIVIMRKFEIGALVELVRAHGVTVAPFVPPIVVEIAKSPRVGADDLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFAKEPFQVKSGSCGTVVRNAELKIVDPDTSAFLGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDELAGEVPVAFIIRSEGSEISENEIKQFVAKEVVFYKRINRVFFTDSIPKNPSGKILRKDLRARLAAGVPTSDNTQSKS
>Adlay0256_T0190
MVSPTEPQAETTVFRSTLPDIAIPDHLPLHDYVLERLAERRDRACLIDGATGETLTFGDVDRLSRRVAAGMRATLGVRSGGTVMLLLPNSVEFALAFLACSRLGAAATTANPLHTPPEIAKQAAASGATVVITEPAFVGKVRGLAGVAVVATGDGAEGCVSFSDLASTDGSALPEAAIDVANDVVALPYSSGTTGLPKGVMLSHRGLVTSVAQLVDGDNPNLHIREDDVVLCVLPMFHVYSLHSILLCGMRAGAALVIMKRFDTLRMFELVKRHGVTVVPLVLPIAVEMAKSDAVDRHDLSSVRMVISGAAPMGKELQDMLRARLPRAVLGQGYGMTEAGPVLSMCMAFAKEPSPVKSGACGTVVRNAELKIVDPETGLSLHRNQPGEICIRGKQLMKGYLNNPEATAKTIDSEGWLHTGDIGYVDDDDEIFIVDRLKELIKYRGFQVAPAELEAMLIAHHSIADAAVVPMKDDYSGEIPVAFVVTSGGSEITEDEIKQYVAKQVVFYKRLHKIFFVEAIPKAPSGKILRKDLRAKLASGFSNGSSC
>Adlay0318_T0070
MGSIDAVVAVPVPAAEPSAAEEKAVVFRSKLPDIEINNSQSLQAYCFGKMGEVADRACLIDGQTGASYTYAEVESLSRRAASGLRAMGVGRGDVVMNLLRNCPEFAFAFLGAARLGAATTTANPFYTPHEIHRQAEAAGARLIVTEACAVEKVREFAAARGVPVVTVDGRFDGCVEFAELIAAEELEADADVHPDDVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGENPNLYFSKDDVLLCLLPLFHIYSLNSVLLAGLRAGSTMVIMRKFDLGALVELVRKYVITIAPFVPPIVVEIAKSPRVTADDLASIRMVMSGAAPMGKELQDAFMAKIPNAMLGQGYGMTEAGPVLSMCLAFAKEPFQVKSGSCGTVVRNAELRIVDPDTGAALGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDDLAGEIPVAFIVRTEGSEVTEDEIKKFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARLAAGVH
>Adlay1127_T0300
MRMFVFLWWINCRRRGMATPSLPSQIVEFNYKTMEDKLKRLALKVSHHEDNIRFLKSQLNAVEEACVDLGIKLGNYHASVAAVVNNDTSAQEAEERTIRTILDQDKTAAGIICQLKVRHHELASKMPLMKDILGFVATLGKVNNDNLSRLLTEYLGMDNMLALVCKTYDGVKGLEKYDKDGIIDKTSGVHGLGRSVGKFLDGRFTVFCLENLSKNLEVKFPVNIEVSSLPENIAEMEEQVKLKNWEKERVLEDMKREGDLLKQVKELYMKQKQEFMDYLAQPAVAQALHDSPTTHSPATPGSNPFGAKPSQKRWLFLLHGLPWKSSLRGQCELNSKDMSMTQPNLTCTTPRKNCVNHMPSPSSRLHQSLPTMPRPPPPLVSSNANAAPFLQLPTKQQQQQLRRRMGDAAIVTTGVHLHEAAEEEQEHIFRSRFPPVAVPDDVTVPEFVLAGAEAYADKTALVEAAPGGRSYTYGEVARDVARFARALRSVGVRRGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRAAAEEIRKQVEDSEARLVVADAVAYDKVKDIIGVPVIGIGGGLLPGAIGWDELLAAADRTGAPVPVVALDAARQSDLCALPYSSGTTGVSKGVMLSHRNLVSSLCSSMFAVGEELVGQVVTLGLMPFFHIYGITGICCATLRHKGTVVVMDRFDLRAFLGALLAHRVMFAPIVPPVMLAMVKSPVADEMDLSGLALRSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGGGEEGGPVQVAKKGSVGFVLPNLEVKFVDPGTGRSLPKNTPGEICVRSQAVMRGYYGRKDETERTVDAAGWLHTGDVGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLSHPSVQDAAVFGLPDEEAGEVPASCVVRRRGAAESEADVMAYVAGRVASYKKLRLLRFVDAIPKSVSGKILRRQLRDDFIINRAAQAQDGGGASTCQVQS
>Adlay1326_T0120
MARTAAPAAGYGADGVYRSPRPAVRIASDPGLSLNDLLFRRADACPSALALVDSATGQSLTFAAFRSAVLTTAVALSSRAGVRRGDVVLFFAPNCFLYPVCFFAVTALGAVATTANPLYTPREVAKQATDSRAKLAITVSELLPKIVGLGLPTILLDGDGDGGAASATAASRPQGVSVTLYSDLISGARETEYRRPPTKQSDTAALLYSSGTTGASKGVVLTHRNFISAAAMVTADQDAHGEGPNVFLCFLPMFHIFGLSVITFAQMQRGNSVVVMSRFDMDSVMAAVQRHRITHLFCVPPVMIALAKLGSVGKYDLSSLRFIGSGAAPLGKDVMEGVANNFPEAVIAQGYGMTETCGIISLEYPEEGQVRQFGSTGALVSGVEAKIVDVETLKCLPPNQLGEICVRGLNIMQGYFNNVQATEFTIKQGWLHTGDIGYFDDGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDAEAGEVPIAYVVRSPVSSLTEVDVQKFIEKQVAYYKKLRRVTFVDSVPKSASGKILRRELIAKVRSSKL
>Adlay1397_T0010
MGSTEEQQQQAAVEEASPEIIFRSKLPDIAISNTLPLHRYCFELLPEVGERPCLIDGATDAVLTYGEVDRLSRRLAAALRRAPLGLERGAVVMNLMLNSAEFVLSFFAASRVGAAVTTANPMSTPHDIAKQIAASGATVVFTESMAVDKLPGGVTVVLIDAHRDGCLHFWDDVIASVPDDEDQEEEAAEAAAGFDPDDVVALPYSSGTTGLPKGVMLTHRSLSTSVAQQVDGDNPNIGFTAADVILCSLPMFHIYSLNTIMMCGLRVGAAIVVMRRFDLARMMQLVERHRITIAPLVPPIVVAVAKSDEAAAHDLSSVRMVLSGAAPMGKDIEDAFMAKLPAAVLGQGYGMTEAGPVLSMCLAFAKEPFKVKSGACGTVVRNAELKIVDPDTGKSLPRNLPGEICIRGQQIMKGYLNNPEATKNSIDADGWLHTGDVGFVDDDDEIFIVDRLKEIIKYKGLQVAPAELEALLITHPSIADAAVVGKQVEPEIGEIPVAFVAKAKGSELNEDDVKQFVAKEVIYYKKVREVIFVDKIPKAPSGKILRKELRKQLQQQQQQLA
>Adlay1868_T0090
MITVAAPEAQPQAAAAPAAPEETVFRSKLPDIDIPSHLPLHDYCFARAAEVADAPCLIAAATGRTYTYAETRLLCRKAAASLHGLGVGQGDRVMVLLQNSVEFVLTFLGASFLGAVTTAANPFCTPLEIHKQLRASGAKLVVTQSAYVDKLRHEAFPRIGAASGGDGNALTVLTTDDDDAAGTGTPPEGCLAFWELVAPADDAALPEVSISPDDPVALPFSSGTTGLPKGVVLTHGGQVSNVAQQVDGANPNLYMREGDVALCVLPLFHIFSLNSVLLCALRAGAAVMLMPRFEMGAMLEGIQRWCVTVAAVVPPLVLALAKNPALEKYDLSSIRIVLSGAAPLGKELVDALRARVPQAVFGQGYGMTEAGPVLSMCPAFAKEPTPAKPGSCGTVVRNAELKVVDPDTGLSLGRNLPGEICIRGPQIMKGYLNDPEATARTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLIAHPSIADAAVVPQKDDAAGEVPVAFVVRAADSDIAEDAIKEYISKQVVFYKRLHKVYFALSIPKSASGKILRRELRAKLAAAATT
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University