Enzyme
Isolated from the plant Gentiana triflora. Transfers the hydroxycinnamoyl group only to the C-5 glucoside of anthocyanin. Caffeoyl-CoA, but not malonyl-CoA, can substitute as an acyl donor.
5AT
Enzyme
Anthocyanin 5-aromatic acyltransferase
Enzyme name
EC:2.3.1.153
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanidin 3,5-di-O-beta-D-glucoside 5-O-glucoside-6'''-O-4-hydroxycinnamoyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3,5-di-O-beta-D-glucoside = CoA + anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [RN:R05163]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [CPD:C06363]
Product
Species information
Coffea canephora
Species name
Robusta coffee (Coffea canephora) is a species of eudicot in the family Rubiaceae (madder family).
Species info
Gene sequence info
Coffea_canephora.5AT.fa
Download
>rna-GSCOC_T00035311001
MSSPGIQHISQCFIKPKNTPEEAKQPIYLSQWDLAMACANYIQKGLLFAKPPAFDRENQMEDLLEKLKDSLALALDHFYPLAGRLATLKQENPPIYSIYVDCNNSPGARFVHASLNSTIDDILSPVDVPKIVQSFFDHDRAINHDGHTRPLLTIQVTELIDGIFMGCSANHMIVDGTSYWHFFNTWSEIFNAKGQKIAISRPPIHKRWFLEGHGPILSLPFTHHDQFVRRHEAPQMRERVFHFSSESLAKLKAKANAESDTTKISSLQALAAHVWRCITRTRNLPPDQETSCMMAINNRTRLCPPVPQEYVGNCIQAVRATAAAGELLDRGLGWSSSKLHLAVHNHTDEIVRNWVESWLQSPVIYQAAEFIDPCSVLMGSSPRFNMYGSEFGLGKAVAIRSGYANKFDGKVSSFPGVEGGGSMDLEICLRPHSMSLLESDEEFMGAVTVSSSRD
>rna-GSCOC_T00016684001
MATSSGARCLTRVEAISAFVWKCSMAASEANSGHRKSSMLTHVVNLRRRAAPTLSEHSIGNLIWISGARCLVNQDRGMPALADQIQYSISKINSDYVKKMHGNEGPALMRKSLKEIGEFGSKEAAVDYLGFSSWCGFGFYEIDFGWGKPIWVSSFAVNAPVFMNLIILMETRLGDGIEAWVTLDQQEMDIMEHDQEFMAFVSVDPSPL
>rna-GSCOC_T00035310001
MSSPGIHHISQCFIKPKNTPEEAKQPIYLSQWDLAMVCVNYIQKGLLFAKPPAFDRENQMEDLLEKLKDSLALALDHFYPLAGRLATLKQENPAIYSIYVDCNNSPGASLVHASLNSTIDDILSPIDVPEIVQSFFDHDRAINHDGHTRPLLTIQVTELTDGIFIGCSINHMIVDGTAFWHFFNTWSEIFSAKGQKTAISRPPIHKHWFPEGHGPILSLPFTHHDQFVRRHEAPQMRERVFHFSSESLAKLKAKANAESSHTTKISSLQALSAHVWRCITRTRNFSPDQETSCRLAINNRTRLHPPVPQEYVGNCIQTVRAAAAAGELLDRGLGWSAWKLHLAVHNHTDEIVRDWVESWLQSRFIYQLAQFFDPCSIMMGSSPRFNMYGNEFGLGKAVAIRSGYANKFDGKVSLYPGIEGGGSMDLEICLLPHYMSLLESDEEFMETVTLSSSRD
>rna-GSCOC_T00039240001
MLFSTMSRTTTVSKCSIFPSEDSTLGDLKLSVSDLPMLSCHYIQKGCLFTRPPFPISDLISLLKRSLSTTLSHFPPLAGRLFTDSDGHIYITSNDAGVDFTHANASHVRIRDILGSIDVPHQVKEFFSLDGMISYEGHFRPILAVQVTELADGVFIGCSLNHAVTDGTSFWNFFNTFAEVSRGIKRISRTPDFSRESVLISPAVLKLPADGPKITFDADAPVRERILSFTRESILKLKAIANNQKCEVNHVVELMGKQRHDPLARLEGKMTPLAQQQNKTRTTEISSFQALSALLWRGVTRARKLPASKTTTFRMAVNCRNRLGPKLEPLYFGNAIQSIPTYASAGDILAHDLRWCAEQLNKNVTAYDDATVRSNVQDWERDPRCFPLGNFDGAMLTMGSSPRFPMYDNDFGWGRPMAVRSGKANKFDGKISAFPGREGGGTVDLEVILAPETMDGLESDQEFMQYVSSGY
>rna-GSCOC_T00042562001
MAQVEIILKEYIKPSSPTPLHLKHQNLSFIDRLPTPIYLPFILFYQNHASSDRSQISQQLKLSISQALTKFYPLAGRIQDDVFVDCNDSGALFIEAQVHSSLSEATQNFVIEEFNHYLPIKPYEIIENSLRNDVPLAVQISFFECGGMSVGVCISHRIADALSVVMFLNSWAAICRGSDDREILTPNFDVGCLHFPPPEDVPRAPLISVADKQKIVAKRFVFSKEKLERIKELASSDSTSTIRDPTRVEALSAFICKNFIEVNRSKLDSETMFVAIHNVNLRPKMKLPHDEFAFGNVSLPISAVLMPEMEKECHNLAGHLRYAIRNVNDDFVKNVVQQREPYLRILSETRKLLSKTEMCYCNFTSWCRFPVYEVDFGWGKPFAIGTAAVPRKNTIILMSTKCGDGIEAWINMAEDHMELLPSDFLSLANHDF
>gene-GSCOC_T00042566001
MCDTTKFNRKPLQHVEVLTKKLVKPSSPTPNHLQNYKLSSFDQLAPRSLVHLVYFYENNDSCKGNYDEAGVVQRHEILQISLAETLSHFRPLAGRISDDGRRSVDCQDQGALYIEAKVNCQLNQFLNQAYEDVELLADFIPDGGLNGDGALLRVRVTFFLCGGFALACSISHTVADGFTGCTFFDEWAKMCGYGKDNNNICDIKCLNASVEFRPSRLEVVTAFIWRGLIRAAQKRHGYLRASMTAISVNLRGKTALGIEDNSFGNLFVAVPIKFIPDETKMELHHFVQLIMTTLQEARTVYTKASSADEIFLMVQKFQDQVQKELGDKEVDTRLSTSLCRFPLYGADFGWGKPSWIIFGNVGGRPEMFCLLDTKCGTGVEVRVNLNEVEMPIFEFDREIVSVTSLAAR
>rna-GSCOC_T00023385001
MPTTAVHVVSKCTVYPESKSALFPSLKLSVSDLPMLSCQYIQKGVLLSQPPLDAASLLSLLKLSLSKALSHFPPLAGRLHTDPHGHVHILCNDAGVDFVHAKALHLSLPTLVPSDQLHLHDIPPCFRKFFQFDHTLSYAGHHKPLLAVQVTELNGGLFIGCTMNHAVVDGTSFWNFFNTFAEACRGAKRISKSPNFCRETVFTSPAVLQLPAGGPSATFSGDEPSREKIFHFSREAVLQLKLRTNKTCKMGSWVAGDGEKGGRTNGKVTRKSLKPATDEISSFQSLCAQLWRSVTRARNLDGNRRTTFRMAVNCRHRLEPRVDPLYFGNLIQSIPTVACVEELLGNDLSWGADRLHRNVLAHDDSTVRRGVEEWESNPRLFPLGNFDGAMITMGSSPRFPMYDNDFGWGRPIAVRSGRANKFDGKISAFPGREGNGSVDLEVVLAPETMAALEKDVEFMQYVS
>gene-GSCOC_T00013760001
MYQVLPAGKTPGGLYFLSNLDQTFPYPVEIVFSYKGKGNGTNKTSAEVLKTELATKVLVEFYPLAGCVATSWDGKMLVRCNGEGVPFVEATCEYEMELLGNINPMNRRELRIVHPLQLSRRVTTFKCGGIVVGVVLNHVLIDGKSFIHFVNSWAEVARGMTLSVLPHLWIDLYYLQGSLQSSILLMMNT
>rna-GSCOC_T00006943001
MDASLQFEIISETVIKPSSPTPPPLRYHKLSSVDQAMPPNYRIRLAFFYANIKGTRWLANEISQLLKSSFSKALAQYYPFAGRLMKNGYMIDCNDMGAQFTEGRINSRVAEIRKQLSSSSEKVQDLIFARSLFSGPASANHRSLVFVQLSSFNCGGIVLSVSINHRIADASSVSTFMNDWAAIARQTSDIPSPHLHGASLFPPVDGQQYRHPFPPKPDQGKYAKRILAFHASKIGELKAAALNSGVENPTRFEVVSALLYSCFMSAASKANSGSHIRPSIFFNAVNFRQIIVPPLPQNSVGNFVTSFLTSVDNNAEVKLPELVNKLREGKTKLRKECTENINAIPSKLKASHSASPTVAIETINSDCYGCSSWCRFPFYGVDFGWGTPSLVYPAIIPEQNANILVLIDMEDGNGIYAHLILKELDMSLLEENEVLLAYASLDA
>gene-GSCOC_T00012299001
MLQTYVAMVQISIFSCGGIAIGLSASHKIVDGLSMSTFMNAWAATARESLEQINASFVSSTLSPGSRYTSKYSKLFTPALDVLKTKATTSSLKPSSVTAVMGLLWKSAIVASQMKLSLLIPVWPAYAQQGTDSSPELQLLVNCIRNAITTIYSNFVEGMKGEDRLQKNFEQLFSNPNSSAEDFIISSLCKTGFREVDFGWGKPIWSFVGRENRNIYGSRVERIAWLMDTKSGDGIEAWVTLKEDGMAIFDKNLELQAFASLNPAPLES
MSSPGIQHISQCFIKPKNTPEEAKQPIYLSQWDLAMACANYIQKGLLFAKPPAFDRENQMEDLLEKLKDSLALALDHFYPLAGRLATLKQENPPIYSIYVDCNNSPGARFVHASLNSTIDDILSPVDVPKIVQSFFDHDRAINHDGHTRPLLTIQVTELIDGIFMGCSANHMIVDGTSYWHFFNTWSEIFNAKGQKIAISRPPIHKRWFLEGHGPILSLPFTHHDQFVRRHEAPQMRERVFHFSSESLAKLKAKANAESDTTKISSLQALAAHVWRCITRTRNLPPDQETSCMMAINNRTRLCPPVPQEYVGNCIQAVRATAAAGELLDRGLGWSSSKLHLAVHNHTDEIVRNWVESWLQSPVIYQAAEFIDPCSVLMGSSPRFNMYGSEFGLGKAVAIRSGYANKFDGKVSSFPGVEGGGSMDLEICLRPHSMSLLESDEEFMGAVTVSSSRD
>rna-GSCOC_T00016684001
MATSSGARCLTRVEAISAFVWKCSMAASEANSGHRKSSMLTHVVNLRRRAAPTLSEHSIGNLIWISGARCLVNQDRGMPALADQIQYSISKINSDYVKKMHGNEGPALMRKSLKEIGEFGSKEAAVDYLGFSSWCGFGFYEIDFGWGKPIWVSSFAVNAPVFMNLIILMETRLGDGIEAWVTLDQQEMDIMEHDQEFMAFVSVDPSPL
>rna-GSCOC_T00035310001
MSSPGIHHISQCFIKPKNTPEEAKQPIYLSQWDLAMVCVNYIQKGLLFAKPPAFDRENQMEDLLEKLKDSLALALDHFYPLAGRLATLKQENPAIYSIYVDCNNSPGASLVHASLNSTIDDILSPIDVPEIVQSFFDHDRAINHDGHTRPLLTIQVTELTDGIFIGCSINHMIVDGTAFWHFFNTWSEIFSAKGQKTAISRPPIHKHWFPEGHGPILSLPFTHHDQFVRRHEAPQMRERVFHFSSESLAKLKAKANAESSHTTKISSLQALSAHVWRCITRTRNFSPDQETSCRLAINNRTRLHPPVPQEYVGNCIQTVRAAAAAGELLDRGLGWSAWKLHLAVHNHTDEIVRDWVESWLQSRFIYQLAQFFDPCSIMMGSSPRFNMYGNEFGLGKAVAIRSGYANKFDGKVSLYPGIEGGGSMDLEICLLPHYMSLLESDEEFMETVTLSSSRD
>rna-GSCOC_T00039240001
MLFSTMSRTTTVSKCSIFPSEDSTLGDLKLSVSDLPMLSCHYIQKGCLFTRPPFPISDLISLLKRSLSTTLSHFPPLAGRLFTDSDGHIYITSNDAGVDFTHANASHVRIRDILGSIDVPHQVKEFFSLDGMISYEGHFRPILAVQVTELADGVFIGCSLNHAVTDGTSFWNFFNTFAEVSRGIKRISRTPDFSRESVLISPAVLKLPADGPKITFDADAPVRERILSFTRESILKLKAIANNQKCEVNHVVELMGKQRHDPLARLEGKMTPLAQQQNKTRTTEISSFQALSALLWRGVTRARKLPASKTTTFRMAVNCRNRLGPKLEPLYFGNAIQSIPTYASAGDILAHDLRWCAEQLNKNVTAYDDATVRSNVQDWERDPRCFPLGNFDGAMLTMGSSPRFPMYDNDFGWGRPMAVRSGKANKFDGKISAFPGREGGGTVDLEVILAPETMDGLESDQEFMQYVSSGY
>rna-GSCOC_T00042562001
MAQVEIILKEYIKPSSPTPLHLKHQNLSFIDRLPTPIYLPFILFYQNHASSDRSQISQQLKLSISQALTKFYPLAGRIQDDVFVDCNDSGALFIEAQVHSSLSEATQNFVIEEFNHYLPIKPYEIIENSLRNDVPLAVQISFFECGGMSVGVCISHRIADALSVVMFLNSWAAICRGSDDREILTPNFDVGCLHFPPPEDVPRAPLISVADKQKIVAKRFVFSKEKLERIKELASSDSTSTIRDPTRVEALSAFICKNFIEVNRSKLDSETMFVAIHNVNLRPKMKLPHDEFAFGNVSLPISAVLMPEMEKECHNLAGHLRYAIRNVNDDFVKNVVQQREPYLRILSETRKLLSKTEMCYCNFTSWCRFPVYEVDFGWGKPFAIGTAAVPRKNTIILMSTKCGDGIEAWINMAEDHMELLPSDFLSLANHDF
>gene-GSCOC_T00042566001
MCDTTKFNRKPLQHVEVLTKKLVKPSSPTPNHLQNYKLSSFDQLAPRSLVHLVYFYENNDSCKGNYDEAGVVQRHEILQISLAETLSHFRPLAGRISDDGRRSVDCQDQGALYIEAKVNCQLNQFLNQAYEDVELLADFIPDGGLNGDGALLRVRVTFFLCGGFALACSISHTVADGFTGCTFFDEWAKMCGYGKDNNNICDIKCLNASVEFRPSRLEVVTAFIWRGLIRAAQKRHGYLRASMTAISVNLRGKTALGIEDNSFGNLFVAVPIKFIPDETKMELHHFVQLIMTTLQEARTVYTKASSADEIFLMVQKFQDQVQKELGDKEVDTRLSTSLCRFPLYGADFGWGKPSWIIFGNVGGRPEMFCLLDTKCGTGVEVRVNLNEVEMPIFEFDREIVSVTSLAAR
>rna-GSCOC_T00023385001
MPTTAVHVVSKCTVYPESKSALFPSLKLSVSDLPMLSCQYIQKGVLLSQPPLDAASLLSLLKLSLSKALSHFPPLAGRLHTDPHGHVHILCNDAGVDFVHAKALHLSLPTLVPSDQLHLHDIPPCFRKFFQFDHTLSYAGHHKPLLAVQVTELNGGLFIGCTMNHAVVDGTSFWNFFNTFAEACRGAKRISKSPNFCRETVFTSPAVLQLPAGGPSATFSGDEPSREKIFHFSREAVLQLKLRTNKTCKMGSWVAGDGEKGGRTNGKVTRKSLKPATDEISSFQSLCAQLWRSVTRARNLDGNRRTTFRMAVNCRHRLEPRVDPLYFGNLIQSIPTVACVEELLGNDLSWGADRLHRNVLAHDDSTVRRGVEEWESNPRLFPLGNFDGAMITMGSSPRFPMYDNDFGWGRPIAVRSGRANKFDGKISAFPGREGNGSVDLEVVLAPETMAALEKDVEFMQYVS
>gene-GSCOC_T00013760001
MYQVLPAGKTPGGLYFLSNLDQTFPYPVEIVFSYKGKGNGTNKTSAEVLKTELATKVLVEFYPLAGCVATSWDGKMLVRCNGEGVPFVEATCEYEMELLGNINPMNRRELRIVHPLQLSRRVTTFKCGGIVVGVVLNHVLIDGKSFIHFVNSWAEVARGMTLSVLPHLWIDLYYLQGSLQSSILLMMNT
>rna-GSCOC_T00006943001
MDASLQFEIISETVIKPSSPTPPPLRYHKLSSVDQAMPPNYRIRLAFFYANIKGTRWLANEISQLLKSSFSKALAQYYPFAGRLMKNGYMIDCNDMGAQFTEGRINSRVAEIRKQLSSSSEKVQDLIFARSLFSGPASANHRSLVFVQLSSFNCGGIVLSVSINHRIADASSVSTFMNDWAAIARQTSDIPSPHLHGASLFPPVDGQQYRHPFPPKPDQGKYAKRILAFHASKIGELKAAALNSGVENPTRFEVVSALLYSCFMSAASKANSGSHIRPSIFFNAVNFRQIIVPPLPQNSVGNFVTSFLTSVDNNAEVKLPELVNKLREGKTKLRKECTENINAIPSKLKASHSASPTVAIETINSDCYGCSSWCRFPFYGVDFGWGTPSLVYPAIIPEQNANILVLIDMEDGNGIYAHLILKELDMSLLEENEVLLAYASLDA
>gene-GSCOC_T00012299001
MLQTYVAMVQISIFSCGGIAIGLSASHKIVDGLSMSTFMNAWAATARESLEQINASFVSSTLSPGSRYTSKYSKLFTPALDVLKTKATTSSLKPSSVTAVMGLLWKSAIVASQMKLSLLIPVWPAYAQQGTDSSPELQLLVNCIRNAITTIYSNFVEGMKGEDRLQKNFEQLFSNPNSSAEDFIISSLCKTGFREVDFGWGKPIWSFVGRENRNIYGSRVERIAWLMDTKSGDGIEAWVTLKEDGMAIFDKNLELQAFASLNPAPLES
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University