Enzyme
Isolated from the plants Perilla frutescens and Gentiana triflora (clustered gentian). Acts on a range of anthocyanidin 3-O-glucosides, 3,5-di-O-glucosides and cyanidin 3-rutinoside. It did not act on delphinidin 3,3',7-tri-O-glucoside. Recombinant Perilla frutescens enzyme could utilize caffeoyl-CoA but not malonyl-CoA as alternative acyl donor.
ANAT
Enzyme
Anthocyanin acyltransferase
Enzyme name
EC:2.3.1.215
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanin-3-O-glucoside 6''-O-acyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3-O-beta-D-glucoside = CoA + an anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [RN:R10237]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [CPD:C20461]
Product
Species information
Coffea canephora
Species name
Robusta coffee (Coffea canephora) is a species of eudicot in the family Rubiaceae (madder family).
Species info
Gene sequence info
Coffea_canephora.ANAT.fa
Download
>rna-GSCOC_T00035185001
MDPPLPSNYFGNCQTVIRKNEKNGKLIGEEGFPIAVESIGEGIHQRLKNNDSLFDDADTWLPGIAGINLDRLVSVAGSPRYNYYNLDFGWGKPKKFEFISIETSGAISLGGSRESDGGIEVGLSLSKPRMDAFTVIFNDRLNSL
>rna-GSCOC_T00035210001
MHQSDVQRLKQIVLEKCPKLSRVSTFTVTCAYVWTCLIKSRGASGEDIKENDLEHFMIGADCRSLIAPPLPANYFGNCLVNRIITAKTVELMQESGFIKAAGLIGEVIREKLHNKGGVLNGAEKWLSEIEGLNPERVVGVAGSPKFAVYDMDFGWGKPRKSEVISIDTTGSISLNECRDGKGDLEVGLSLPKVFASGIMEAKDQH
>rna-GSCOC_T00035204001
MMISSRTMKVLQHFLTWDLPKRKPIYNPSFSTFSHMTQMTSSHTVKVLQCCQISPPQGSVPSTTIPLTFFDIPWLLFTPNQPLFFYDFPNFSTHDFIHNILPNLNHSLSSTLQYFFPLAGNLVIPPQPSKPHLSYAEGDSVSLIIAEFSCDFAFLSSYQPRNVQDFYQLVPQLALSKPVETTTRLPLLAVQITIFPRFGVCMGFALRQVAADGRTFDNFLKTWASILGEAGNKCPRLALADNFLPCNDRTLIHDPCGLEQIFLKQWWHLKSLQKDVVEYANGSNFDEIVRATFVVGPIEMEKIKGWIMTRSMNIFGSTRLYLSPYVVTCAFVWVCWTKIHLIHGGDFPEKPHYFGFIAGGITRLGFSVPKSYIGNCVAFGRSMVKRDQLVGENGLIYAAKAIGDTIKELDREVLGEADKWISDWEVLFGSEPHVMITGSPKVGSFELDFGWGRPTKVEEASISKTRAISLCESRDVFHGIEVGLALPKAKMDAFDSLFREGLKTL
>rna-GSCOC_T00035207001
MAPSGEPTTVLDRCLATPPPGLVSRMSLRLSFFDVLWLHAPPVQRLVFYEIPEITKTNFIEEIIPKLRDSLNLTLKYYIPLAGNLIIPPNSGPDSPEILYKRGNSVPLIVAESNAMDFEWLVANHARDSSDFHCLVPKLVQSTDDSGSILSSVLALQVTLFPNMGFSIGITSHQAAGDACTIFRFSRIWAFHAKAGEAEAEEKVNAPCGGPLGPPCYYRTIIKDPKGLSSIFWNRWNSISKLDRGSGISSNSYKVRQTFLISPEQVENIRKLVPPMLRGQAYMKSFKVVCAYVWICLVKSRGEEAVDEEEVENFVCFGNCRRRLDHLVAENYFGNCITLCIAKMKNGELVGGGGIPRAVGLIGAAMNEKLQSQEALSNGAENWLVDLQNLNLDRTFMVAGSPKFNFYQLDFGWGRPRRFEFVSIDKTGAVSLCGGRDRNRDIEVGLSLPKARMDAFAAIFNQGLKDL
>rna-GSCOC_T00035195001
MATPDAVTVLKHSLVSPPSSAAATEMSLPLTFLDMPWLHFSPIQRLVLYEVPQLSRAHFIEHIIPKLEHSLSLTLQHFLPLAGNLIVPSNSNSGTPEIRYKNGSSLALIIAECTTTDFNYLTANHSRNCCDFHPLIPELKPSNQDDSGSTAWTTPVLALQVTLFPHCGMSVGISNHHTVGDAGSVFRFMKTWAALSSKFFEDNIDRDDAAATLVSYSPPCYDRTMIKDTKGLGSIFWDQGVLLIKLFQNESSTDTTAKPMTKKVRRSFVISRNNIEKLKRLALQKRPQLVHLSSFTVICAHVWTCLVKCRGPSGEYVDDEEVEYFCCTADCRGRMDPPLPSNYFGNCQTVIRKNEKNGKLIGEEGFPIAVESIGEGIHQRLKNNDSLFDDADTWLPGIAGINLDRVVSVAGSPRYNYYNLDFGWGKPKKFEFISIETSGAISLGGSRESDGDIEVGLSLSKPRMDAFTVIFNDRLNSL
MDPPLPSNYFGNCQTVIRKNEKNGKLIGEEGFPIAVESIGEGIHQRLKNNDSLFDDADTWLPGIAGINLDRLVSVAGSPRYNYYNLDFGWGKPKKFEFISIETSGAISLGGSRESDGGIEVGLSLSKPRMDAFTVIFNDRLNSL
>rna-GSCOC_T00035210001
MHQSDVQRLKQIVLEKCPKLSRVSTFTVTCAYVWTCLIKSRGASGEDIKENDLEHFMIGADCRSLIAPPLPANYFGNCLVNRIITAKTVELMQESGFIKAAGLIGEVIREKLHNKGGVLNGAEKWLSEIEGLNPERVVGVAGSPKFAVYDMDFGWGKPRKSEVISIDTTGSISLNECRDGKGDLEVGLSLPKVFASGIMEAKDQH
>rna-GSCOC_T00035204001
MMISSRTMKVLQHFLTWDLPKRKPIYNPSFSTFSHMTQMTSSHTVKVLQCCQISPPQGSVPSTTIPLTFFDIPWLLFTPNQPLFFYDFPNFSTHDFIHNILPNLNHSLSSTLQYFFPLAGNLVIPPQPSKPHLSYAEGDSVSLIIAEFSCDFAFLSSYQPRNVQDFYQLVPQLALSKPVETTTRLPLLAVQITIFPRFGVCMGFALRQVAADGRTFDNFLKTWASILGEAGNKCPRLALADNFLPCNDRTLIHDPCGLEQIFLKQWWHLKSLQKDVVEYANGSNFDEIVRATFVVGPIEMEKIKGWIMTRSMNIFGSTRLYLSPYVVTCAFVWVCWTKIHLIHGGDFPEKPHYFGFIAGGITRLGFSVPKSYIGNCVAFGRSMVKRDQLVGENGLIYAAKAIGDTIKELDREVLGEADKWISDWEVLFGSEPHVMITGSPKVGSFELDFGWGRPTKVEEASISKTRAISLCESRDVFHGIEVGLALPKAKMDAFDSLFREGLKTL
>rna-GSCOC_T00035207001
MAPSGEPTTVLDRCLATPPPGLVSRMSLRLSFFDVLWLHAPPVQRLVFYEIPEITKTNFIEEIIPKLRDSLNLTLKYYIPLAGNLIIPPNSGPDSPEILYKRGNSVPLIVAESNAMDFEWLVANHARDSSDFHCLVPKLVQSTDDSGSILSSVLALQVTLFPNMGFSIGITSHQAAGDACTIFRFSRIWAFHAKAGEAEAEEKVNAPCGGPLGPPCYYRTIIKDPKGLSSIFWNRWNSISKLDRGSGISSNSYKVRQTFLISPEQVENIRKLVPPMLRGQAYMKSFKVVCAYVWICLVKSRGEEAVDEEEVENFVCFGNCRRRLDHLVAENYFGNCITLCIAKMKNGELVGGGGIPRAVGLIGAAMNEKLQSQEALSNGAENWLVDLQNLNLDRTFMVAGSPKFNFYQLDFGWGRPRRFEFVSIDKTGAVSLCGGRDRNRDIEVGLSLPKARMDAFAAIFNQGLKDL
>rna-GSCOC_T00035195001
MATPDAVTVLKHSLVSPPSSAAATEMSLPLTFLDMPWLHFSPIQRLVLYEVPQLSRAHFIEHIIPKLEHSLSLTLQHFLPLAGNLIVPSNSNSGTPEIRYKNGSSLALIIAECTTTDFNYLTANHSRNCCDFHPLIPELKPSNQDDSGSTAWTTPVLALQVTLFPHCGMSVGISNHHTVGDAGSVFRFMKTWAALSSKFFEDNIDRDDAAATLVSYSPPCYDRTMIKDTKGLGSIFWDQGVLLIKLFQNESSTDTTAKPMTKKVRRSFVISRNNIEKLKRLALQKRPQLVHLSSFTVICAHVWTCLVKCRGPSGEYVDDEEVEYFCCTADCRGRMDPPLPSNYFGNCQTVIRKNEKNGKLIGEEGFPIAVESIGEGIHQRLKNNDSLFDDADTWLPGIAGINLDRVVSVAGSPRYNYYNLDFGWGKPKKFEFISIETSGAISLGGSRESDGDIEVGLSLSKPRMDAFTVIFNDRLNSL
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University