Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Coffea canephora
Species name
Robusta coffee (Coffea canephora) is a species of eudicot in the family Rubiaceae (madder family).
Species info
Gene sequence info
Coffea_canephora.ANR.fa
Download
>gene-GSCOC_T00041203001
MQKTVCVTGASGFIASWLVKFLLHRGYIVKASVRDLNDPKKVAHLLALDGASERLQLFKANLLEEGSFDASIDGCDGVFHIASPFHDPRNDPQAELIDPAVKGTLNVLKSYFCKETKSWYILAKTLAEDEAWKFVKENDIDMVAINPAMVIGPLLQPTLNTSGAVILNLINGAETFPNSTFPWVDVKDVANAHILAFENPLASGRYCLVEQVAHYSKVMKILHELFPSLKLPVKCADDKPFVPEYQVSKEKAKSLGIEFTPFEQSIKETVESLKEKKLLVASVAL
>gene-GSCOC_T00007483001
MSSVAAGEGKVVCVTGASGYIASWLVKLLLERGYTVKASVRDLNDPKKTQHLATLAGAKERLHLFSANLLEEGSFDAIVEGCEGVFHTASPVQLSVSNPEMHNPLPEGHGKKTVICFVLHYLGYLYMRSLVNDLAIATSEIQLEESNKSLLWPANLLQHLSYFLSLCFSSHDFTVLSLLMFETSRESAELLEPAVRGTVNVLRSCAKVSSIKKVVITSSMAAVTSNRELKKDVVVDESWFSDPLYCEEKKVCYHPFLSVKLCICGMLDFCSFNLLLYKI
MQKTVCVTGASGFIASWLVKFLLHRGYIVKASVRDLNDPKKVAHLLALDGASERLQLFKANLLEEGSFDASIDGCDGVFHIASPFHDPRNDPQAELIDPAVKGTLNVLKSYFCKETKSWYILAKTLAEDEAWKFVKENDIDMVAINPAMVIGPLLQPTLNTSGAVILNLINGAETFPNSTFPWVDVKDVANAHILAFENPLASGRYCLVEQVAHYSKVMKILHELFPSLKLPVKCADDKPFVPEYQVSKEKAKSLGIEFTPFEQSIKETVESLKEKKLLVASVAL
>gene-GSCOC_T00007483001
MSSVAAGEGKVVCVTGASGYIASWLVKLLLERGYTVKASVRDLNDPKKTQHLATLAGAKERLHLFSANLLEEGSFDAIVEGCEGVFHTASPVQLSVSNPEMHNPLPEGHGKKTVICFVLHYLGYLYMRSLVNDLAIATSEIQLEESNKSLLWPANLLQHLSYFLSLCFSSHDFTVLSLLMFETSRESAELLEPAVRGTVNVLRSCAKVSSIKKVVITSSMAAVTSNRELKKDVVVDESWFSDPLYCEEKKVCYHPFLSVKLCICGMLDFCSFNLLLYKI
Sheng jun & Dong yang Teams, Yunnan agriculture University