Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Coffea canephora
Species name
Robusta coffee (Coffea canephora) is a species of eudicot in the family Rubiaceae (madder family).
Species info
Gene sequence info
Coffea_canephora.4CL.fa
Download
>gene-GSCOC_T00024147001
MQECAKNFPHALVLQGYGMTETCGIVSVENSNLGARHSGSAGMLVPGVEAQIVSVEKSKPLPPGKLGEIWVRGPNMMQGYFNNPQATKLTIDKLGWVHTGDLGYFDEEGQLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAEAGEVPVAYVVRSPNSSLTEEDIKKYIADQVAPFKRLRRVTFINSVPKSASGKILRRELIEKVRSKV
>gene-GSCOC_T00022309001
MATSASRGAQTVNSMYFKPTLRKAFHRKSNPPDTAKLNGEEMKRKKKKSSVVGNNHDGNWWVPDDRTGIFYPKGQEKVMEDEPRILILPHLQYGLPGEQNNYIAPPFDWFHFLSLLISSLARSNPSTSPLSMAVKTKQEEIIFRSRLPDIYIPKHLPLHTYCFEDLPKFRSQACLINGATDEIYTFEQVELTARRVASGLNKVGIQQGDTIMILLPNSPEFVFAFLGASFRGAISTMANPYFTSAEVIKQAKASNAKLIITQGCYVEKVRDYACENGVKVVCIDSAPEGCLHFSELTEADEREMPDVEISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNFYIHNQVMMCVLPLFHIYSLNSILLCGLRAGTTILIMQKFDIIPFLELIQKYKVTTGPFVPPIVLAIAKSPEVDKYDLSSVKTVMSGAAPLGKELEDAVRTKFPKAKLGQGYGMTEAGPVLAMCSAFAKDPFEVKSGGCGSVVRNAEMKIVDPETGSSLPRNQPGEICIRGDQIMKGYLDDPEATKATIDEDGWLHTGDVGYIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLAHSDISDAAVVPMKDDAAGEVPVAFVVKSKESNITEDEIKEYIKKQVIFYKRINRVFFVDAIPKSPSGKILRKDLRARLAAGVPK
>gene-GSCOC_T00042531001
MAKAPKIDPRNGFCSETKIFHSLRPPVPLPPPSEPISITDHTRSLLHASTTPTTPFLIDSATDHRISYADFLNQVQSLSLSLQSHFPSLSQNDVAFIFCPPSLHVPVLYFSLLSLGIIVSPANPLSSTSELTHMLQLTKPSIVFTTSSLCHKFPETLKSINSVFVDQPQFQSMLSDTTTGSGVVVIHQNDSAAILYSSGTTGRVKGVELSHRTLIAVIAGLYHNKHKADGEDENPLFPFDFENMLEAIEKYKVTYMPVAPPLVVAFAKSDLVNKYDLSSLRLLACGGAPLGKEVSESFTARFPIGYGMTETGGAATRMIGTEEIKKHGSTGRLSEHTEAKIVDPDTKEALPPGQRGELWLRGPIIMKGYVGDSQATAETLDSEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSIPEVADAAVIPYPDEEAGQIPMAFIVRKPGSHVTETQIMDIVAKQVAPYKKIRRVAFISSIPKSPAGKILRRELVNHAISAASSKL
>gene-GSCOC_T00023742001
MTTPFYGKDGIYRSQKPPVVLPEDPNLSMVPFLFRNFPSLSQSTALIDAGTAESFTFFDLLTQVSKLSHALLKLNINKNDVVLIFSPNSVLFPISFLAVVGIGAIATTVNPLYTINELSNQVNDSNPKLIITGSFDAADSYNTLFCHYSDLINSTSPASSDSLWSSAVQTDVAALLYSSGTTGKSKGVVLTHRNFIATASMVTRDQECYGNSKNVYLCFLPMFHIFGLSVAVYAQLQKGNTVVVMERYEMEKALHAVEKYKVTHMYAVPPVVVALGKQKKVVSKYDCSSLREIACGAAPLGKDVIEECAKNFPQAVIVQGYGVTETCGIISIEDVKTGPPHSGSTGVLFPGVECKILDVDTAEPLPPFQKGQILVRGQNMMQGYFKNQIATNETIDKQGWVHTGDLGYFDDKGLLYVVDRIKELIKYKGFQVAPAELEELLLTHPEISDAAVIPLPDAEAGEIPVAFVVRSSNSSLTEREVQNFVTPYKRLHRVIFSRSIPKSASGKILRRKLRQRAQSNL
>gene-GSCOC_T00041408001
METKQDQQEFVFRSKLPDIYIPNHLPLHSYCFENIPEFCNRPCLINGTTGDIYTYADVELIAREVIMLLLQNSPEFAFALLGASYRGAMSTTANPFYKPAEIEKQARASKAKLIITQSCYVEKVMDFAKENNVKVMCTDAPPEGCLHFSELSSADEKVIPAVKINPNDAVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFHIYSLNSVLLCGLRVGAAILIMQKFEINALMELVQKYKVTIAPFVPPIVLEIAKSPVVDKYDLSSIRMVMSGAAPMGKELEDTVRAKLPKAVLGQGYGMTEAGPLLSMCLAFAKEPFDVKSGACGTVVRNAEMKIVDPETNLSLPRNQAGEICIRGDQIMKGYLNDPEATENTIDKEGWLHTGDIGYIDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLLSHPGISDAAVVSMKDEAAGEVPVAFVVRASGSKISEDEIKQFISNQVIFYKRIHRVFFMDKIPKAPSGKILRKDLRAKLAAEVACN
>gene-GSCOC_T00039644001
MGSTGIQSLHPEEHVFRSRYPPVQIPSDLTLPDFVLQNAELYSEKVAFVDAATGKGYTYAEVARDIRRFAKALRSIGLRKGCVVVVVLPNVIEYAIIALGIMSAGGVFSGANPASHASEIKKQVESAEAKLIITDASTYKKVNDLGLSVIVVGEEERIEGTMKWDELLEAADRASSGSNNETVQQSDLCALPFSSGTTGLSKGVMLTHRNIVANLCSSLFSVGPEMIGQVTILGLIPFYHIYGLTGICCATIRNKGKVVVMPKYELRAFMDALITHEVTFAPIVPPIILGLVKNPIVDEFDLTKLKLRSIMTAAAPLAPEILNEFEKKFPGIVVQEASIAYGMTEHSCITISHGDPSKGHGIAKRNSVGFILPNLEVKFVDPETGRSLPKNTPGEICVRSQCVMKGYYKNEHETALTIDKDGWLHTGDVGYIDDDGDVFVVDRIKELIKYKGFQVAPAELEGVLLTHPSVEDAAVVGLPDKEAGEIPSAWVVISPGAKENEEDIIKHVAANVAHYKKVRRLQFVDAIPKSPSGKIMRRLIKEKMAEI
MQECAKNFPHALVLQGYGMTETCGIVSVENSNLGARHSGSAGMLVPGVEAQIVSVEKSKPLPPGKLGEIWVRGPNMMQGYFNNPQATKLTIDKLGWVHTGDLGYFDEEGQLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAEAGEVPVAYVVRSPNSSLTEEDIKKYIADQVAPFKRLRRVTFINSVPKSASGKILRRELIEKVRSKV
>gene-GSCOC_T00022309001
MATSASRGAQTVNSMYFKPTLRKAFHRKSNPPDTAKLNGEEMKRKKKKSSVVGNNHDGNWWVPDDRTGIFYPKGQEKVMEDEPRILILPHLQYGLPGEQNNYIAPPFDWFHFLSLLISSLARSNPSTSPLSMAVKTKQEEIIFRSRLPDIYIPKHLPLHTYCFEDLPKFRSQACLINGATDEIYTFEQVELTARRVASGLNKVGIQQGDTIMILLPNSPEFVFAFLGASFRGAISTMANPYFTSAEVIKQAKASNAKLIITQGCYVEKVRDYACENGVKVVCIDSAPEGCLHFSELTEADEREMPDVEISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNFYIHNQVMMCVLPLFHIYSLNSILLCGLRAGTTILIMQKFDIIPFLELIQKYKVTTGPFVPPIVLAIAKSPEVDKYDLSSVKTVMSGAAPLGKELEDAVRTKFPKAKLGQGYGMTEAGPVLAMCSAFAKDPFEVKSGGCGSVVRNAEMKIVDPETGSSLPRNQPGEICIRGDQIMKGYLDDPEATKATIDEDGWLHTGDVGYIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLAHSDISDAAVVPMKDDAAGEVPVAFVVKSKESNITEDEIKEYIKKQVIFYKRINRVFFVDAIPKSPSGKILRKDLRARLAAGVPK
>gene-GSCOC_T00042531001
MAKAPKIDPRNGFCSETKIFHSLRPPVPLPPPSEPISITDHTRSLLHASTTPTTPFLIDSATDHRISYADFLNQVQSLSLSLQSHFPSLSQNDVAFIFCPPSLHVPVLYFSLLSLGIIVSPANPLSSTSELTHMLQLTKPSIVFTTSSLCHKFPETLKSINSVFVDQPQFQSMLSDTTTGSGVVVIHQNDSAAILYSSGTTGRVKGVELSHRTLIAVIAGLYHNKHKADGEDENPLFPFDFENMLEAIEKYKVTYMPVAPPLVVAFAKSDLVNKYDLSSLRLLACGGAPLGKEVSESFTARFPIGYGMTETGGAATRMIGTEEIKKHGSTGRLSEHTEAKIVDPDTKEALPPGQRGELWLRGPIIMKGYVGDSQATAETLDSEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSIPEVADAAVIPYPDEEAGQIPMAFIVRKPGSHVTETQIMDIVAKQVAPYKKIRRVAFISSIPKSPAGKILRRELVNHAISAASSKL
>gene-GSCOC_T00023742001
MTTPFYGKDGIYRSQKPPVVLPEDPNLSMVPFLFRNFPSLSQSTALIDAGTAESFTFFDLLTQVSKLSHALLKLNINKNDVVLIFSPNSVLFPISFLAVVGIGAIATTVNPLYTINELSNQVNDSNPKLIITGSFDAADSYNTLFCHYSDLINSTSPASSDSLWSSAVQTDVAALLYSSGTTGKSKGVVLTHRNFIATASMVTRDQECYGNSKNVYLCFLPMFHIFGLSVAVYAQLQKGNTVVVMERYEMEKALHAVEKYKVTHMYAVPPVVVALGKQKKVVSKYDCSSLREIACGAAPLGKDVIEECAKNFPQAVIVQGYGVTETCGIISIEDVKTGPPHSGSTGVLFPGVECKILDVDTAEPLPPFQKGQILVRGQNMMQGYFKNQIATNETIDKQGWVHTGDLGYFDDKGLLYVVDRIKELIKYKGFQVAPAELEELLLTHPEISDAAVIPLPDAEAGEIPVAFVVRSSNSSLTEREVQNFVTPYKRLHRVIFSRSIPKSASGKILRRKLRQRAQSNL
>gene-GSCOC_T00041408001
METKQDQQEFVFRSKLPDIYIPNHLPLHSYCFENIPEFCNRPCLINGTTGDIYTYADVELIAREVIMLLLQNSPEFAFALLGASYRGAMSTTANPFYKPAEIEKQARASKAKLIITQSCYVEKVMDFAKENNVKVMCTDAPPEGCLHFSELSSADEKVIPAVKINPNDAVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFHIYSLNSVLLCGLRVGAAILIMQKFEINALMELVQKYKVTIAPFVPPIVLEIAKSPVVDKYDLSSIRMVMSGAAPMGKELEDTVRAKLPKAVLGQGYGMTEAGPLLSMCLAFAKEPFDVKSGACGTVVRNAEMKIVDPETNLSLPRNQAGEICIRGDQIMKGYLNDPEATENTIDKEGWLHTGDIGYIDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLLSHPGISDAAVVSMKDEAAGEVPVAFVVRASGSKISEDEIKQFISNQVIFYKRIHRVFFMDKIPKAPSGKILRKDLRAKLAAEVACN
>gene-GSCOC_T00039644001
MGSTGIQSLHPEEHVFRSRYPPVQIPSDLTLPDFVLQNAELYSEKVAFVDAATGKGYTYAEVARDIRRFAKALRSIGLRKGCVVVVVLPNVIEYAIIALGIMSAGGVFSGANPASHASEIKKQVESAEAKLIITDASTYKKVNDLGLSVIVVGEEERIEGTMKWDELLEAADRASSGSNNETVQQSDLCALPFSSGTTGLSKGVMLTHRNIVANLCSSLFSVGPEMIGQVTILGLIPFYHIYGLTGICCATIRNKGKVVVMPKYELRAFMDALITHEVTFAPIVPPIILGLVKNPIVDEFDLTKLKLRSIMTAAAPLAPEILNEFEKKFPGIVVQEASIAYGMTEHSCITISHGDPSKGHGIAKRNSVGFILPNLEVKFVDPETGRSLPKNTPGEICVRSQCVMKGYYKNEHETALTIDKDGWLHTGDVGYIDDDGDVFVVDRIKELIKYKGFQVAPAELEGVLLTHPSVEDAAVVGLPDKEAGEIPSAWVVISPGAKENEEDIIKHVAANVAHYKKVRRLQFVDAIPKSPSGKIMRRLIKEKMAEI
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University