Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Citrus sinensis
Species name
Sweet orange (Citrus sinensis) is a species of eudicot in the family Rutaceae (rue family).
Species info
Gene sequence info
Citrus_sinensis.FLS.fa
Download
>rna-XM_006465680.3
MAVSPESLPEKPLDFRAPPPSPIASGRRSCVTNDDVLTEFLEHSLRVPDLILPDKVFPRQKFIENPPSIDFQSLRSMQSDAVSKLLDSIATIGCFQLVNYGVPIEFINSTMATAGGVFGVSSEKRAAVTRSPEKPYGFEEVHGEEEENEFSEEFVWCRDESLKQEMEGVWPLGYSKFSEKIETLMLDMEKVAEKILQVVRENSERKSMNGNCMEQEQENVGSVCYLYRHCRDVPADSSSLRYDVIRMLIRGVDFSHALCLHICDGASEFHVYSKKGWVSFTPDKDAIVITAGDQIQAFGGGQLKHVIGRPIFKTDQKEDCISMAVLYSPPSITSSSSSITYIPEKGKTISLRQQFMIAIFLTLLYHFLVYVYKKI
>rna-XM_006466120.1
MEVERVQAIASLSHSNGTIPAEFIRPEKEQPASTTYHGPAPEIPTIDLDDPVQDRLARSIAEASREWGIFQVTNHGIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFTYLSLGLGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ
>rna-XM_006472674.1
MEGEVRVQDVALKHNDTIPQEFIRSEHEQPGITTVHGAVLEVPVIDLKDPDQEKVHRSIVDASQQWGMFQVVNHGIPGEVIRELQEVGKMFFELPQEEKEKYAKSPDSKDIQGYGSKLQKELEGKKGWVDHLFHKIWPPSAIDYRFWPENIPSYREANEEYRKYLHGVADKLLKCLSLGLGLEENDVKEALGGDNLIYLLKINYYPPCPQPELALGVVAHTDMSSITLLVPNDVQGLQASRDGHWYDVKYIPNALVIHIGDQMEILSNGKYKSVLHRTTVTKDKTRMSWPVFLEPPPDQEVGPHPKLVNEENPAKFKTKKYSDYAYCKLNKIPQ
>rna-XM_006485409.3
MKMEVERGVQANIGSLSHSNEQVEPEAAVRTCHNGTVSEIPTIDLNDPDQERLTGAIAEASQEWGIFQVINHGIPSELINKLQGVGREFFELPQEEKEAYARPRDAKDIEGYGTRLQKEAEEKKSWVDHIFHRIWPPASRNYRFWPKNPPSYREVNEEYANCMRGVVNKLFRCLSLGLGVEGHALKEAVGGDELEYMLKINYYPPCPRPDLAPGLVPHTDLSSITILVPNDVPGLLAFKGDRSIDVNYIPNALIVTIGDQIEILSNGKYKAVLHKATPHKEKTRISWPVFLDPPADMVVGPLPQLVNGKNPPKYEAKKYKDYVHFKLDELNKKWLLF
MAVSPESLPEKPLDFRAPPPSPIASGRRSCVTNDDVLTEFLEHSLRVPDLILPDKVFPRQKFIENPPSIDFQSLRSMQSDAVSKLLDSIATIGCFQLVNYGVPIEFINSTMATAGGVFGVSSEKRAAVTRSPEKPYGFEEVHGEEEENEFSEEFVWCRDESLKQEMEGVWPLGYSKFSEKIETLMLDMEKVAEKILQVVRENSERKSMNGNCMEQEQENVGSVCYLYRHCRDVPADSSSLRYDVIRMLIRGVDFSHALCLHICDGASEFHVYSKKGWVSFTPDKDAIVITAGDQIQAFGGGQLKHVIGRPIFKTDQKEDCISMAVLYSPPSITSSSSSITYIPEKGKTISLRQQFMIAIFLTLLYHFLVYVYKKI
>rna-XM_006466120.1
MEVERVQAIASLSHSNGTIPAEFIRPEKEQPASTTYHGPAPEIPTIDLDDPVQDRLARSIAEASREWGIFQVTNHGIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFTYLSLGLGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ
>rna-XM_006472674.1
MEGEVRVQDVALKHNDTIPQEFIRSEHEQPGITTVHGAVLEVPVIDLKDPDQEKVHRSIVDASQQWGMFQVVNHGIPGEVIRELQEVGKMFFELPQEEKEKYAKSPDSKDIQGYGSKLQKELEGKKGWVDHLFHKIWPPSAIDYRFWPENIPSYREANEEYRKYLHGVADKLLKCLSLGLGLEENDVKEALGGDNLIYLLKINYYPPCPQPELALGVVAHTDMSSITLLVPNDVQGLQASRDGHWYDVKYIPNALVIHIGDQMEILSNGKYKSVLHRTTVTKDKTRMSWPVFLEPPPDQEVGPHPKLVNEENPAKFKTKKYSDYAYCKLNKIPQ
>rna-XM_006485409.3
MKMEVERGVQANIGSLSHSNEQVEPEAAVRTCHNGTVSEIPTIDLNDPDQERLTGAIAEASQEWGIFQVINHGIPSELINKLQGVGREFFELPQEEKEAYARPRDAKDIEGYGTRLQKEAEEKKSWVDHIFHRIWPPASRNYRFWPKNPPSYREVNEEYANCMRGVVNKLFRCLSLGLGVEGHALKEAVGGDELEYMLKINYYPPCPRPDLAPGLVPHTDLSSITILVPNDVPGLLAFKGDRSIDVNYIPNALIVTIGDQIEILSNGKYKAVLHKATPHKEKTRISWPVFLDPPADMVVGPLPQLVNGKNPPKYEAKKYKDYVHFKLDELNKKWLLF
Sheng jun & Dong yang Teams, Yunnan agriculture University