Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Citrus sinensis
Species name
Sweet orange (Citrus sinensis) is a species of eudicot in the family Rutaceae (rue family).
Species info
Gene sequence info
Citrus_sinensis.HI4_OMT.fa
Download
>rna-XM_006467652.3
MGDIENQGVTLREMFEGQVHLKKGVIKILSSMSLKCAVELGIADAIHSHGRPITLSELASALNIQPTKTRSLFHFMRLLVHMGLFSKTKVDNHNEQEEAYGLTPTSTLLIKDKPYCLSPLVTGLLDQDYVSSFQHLSRWFKGNDLTLWESFGGLKFWDYLNQNTALTKRFNQAMESDSEMATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKECREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAGFTHYKVAPIFGIKSLIEVYP
>rna-XM_006467653.3
MGDIENQGVTLREMFEGQVHLKKGVIKILSSMSLKCAVELGIADVIHSHGRPITLSELASALNIQPTKTRSLFHFMRLFVHVGLFLKTKVVNHNEQEEAYGLTPTSTLLIKDNSYCLSPLVTGLLDQDFVSSFQYLSSWFKANDLTLWETVRGLKFWDYLNQNPGLSQQFNQVIESDSVMATLLVKDCRPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYIEGDMFQFIPPSDAFFFKTVFHFFDDEDCLKLLKKCREAIASNGERGKVLIIDIVIDEKEDDRELTESKLLFDIFMNFNVGGKERTEQEWGSLFVNAGFTHYKIAPIFGIKSLIEVYP
>rna-XM_025094656.1
MGDIENDGVTVRELFEGQAHLYKGIVKNLSSMSVKCAVELGIADAIHSHGRPITLSELASALNIQPTKTRSLFHFMSLLVHMGMFSKTEVDNHNEQEEAYGLTPTSTLPSKISPTHLSRWFKGNDITLWETFRGLKFWDYLNQNTALTKRFNQAMASDSKMATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLELPHAVTDMPQTENLKYVEGDMFQFIPPADAFFFKTVFHLFDDEDCLKLLKECREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAGFTHYKVAPIFGIKSLIEVYP
>rna-XM_006468285.3
MDPIQNEGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVQSAQQQQEEAYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMFQSIPPADAFFFKAIFHAFVDEDCLKILKRCREAIASRGDRGKVIIIDIVINEKKEDAQLTEAKLLYDMLMMVAVRGSERTEKEWEKLFLDAGFSHFKITPVYGIKSLIEVYP
>rna-XM_006471086.3
MSSIEVEDANELFQAQAHLYKHVLSYMSSIFLKCAVELGIPDIIHKRGRPVTLPELVSALEFQPNKRNCLRRIMRLLDHSGFFSTTKVHNSREEENEAYALTSASKLLLKDKPYCLSPFVLLVTDAAFITPGHYLSRWLRGNELPDPFVTGHGINIWGYAEQNHEFNNLFNQGLASDSQMAKLIIKDCKHIFDGLSSLVEVGGGTGSFARIISEAFPSIKCSVLELPHVIADLPETDNLKFIAGDMNQSIPSADAFLFKLIFHDYDDEVCLKLLKNCREAVASSDGREKVIIVDIVVNEKKDKPEITEAKLLYDALMMTCVPGIERSEKEWERLFFDAGFTSYKITPLLGLRSFIEVYL
>rna-XM_025102642.1
MDANQDQGAKELFQGQAQLYKLMFNHLSSMSLKCAIELGIADIIHSHGRAITLSELVSALDIQPTKTTGLFKLMRLLVHSGCFNKTKVNGQEEAYGLTAASTLLIKDKPYCFSPNVSLFLDPCFVGAFQSLSSWFKGTELTLWETVHGIKFWEFMNQNPGINQRFNEAMASDTEIMTSFVVKAECKQIFEGLGSLVDVGGGNGSLSRIISEAFPGIKCTVLDLPHVVANLPEADNLKYIAGDMFQFIPPADAFLFKLVFHGLDDEDGLKILKKRREAIASNGEGGKVIIIDIVINAEEEEHELTETKFLFDILMSVNANGKERTESEWAKLFSDAGFSHYKITPIFGMRSLIEVYP
>rna-XM_006491981.2
MDTIENEGGISVRELFEGHGQVIRLVFNHLSAMSLKGAVELGIADIIHSHGRPITLSELVSALKIQPTKTSNLFRFMRLLVHMGLFSKTKVGGEKEEEAYGLTAISALLIKDKSYCLSPLVSGILDPENIFPLHFISKWFKGNDISVWETVHGKNYWDYMNQNPGLSQRFNQAMISDSEMAIFIVKDCCRKIFEGLGSIVDVGGGNGGFSKIISEAFPGIKCTVLDLPHAVANMPQTENLKYIAGDMFQYIPPADAYFFKLVFHAFGDEDCLKILKKCREAIAGNGERGKVLIMDIVINEKEDKHQVTEAKLLGDTLMSVSVDGKERTDEEWKTLFLDAGFTHYKITNVFGLKSLIEVYP
MGDIENQGVTLREMFEGQVHLKKGVIKILSSMSLKCAVELGIADAIHSHGRPITLSELASALNIQPTKTRSLFHFMRLLVHMGLFSKTKVDNHNEQEEAYGLTPTSTLLIKDKPYCLSPLVTGLLDQDYVSSFQHLSRWFKGNDLTLWESFGGLKFWDYLNQNTALTKRFNQAMESDSEMATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKECREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAGFTHYKVAPIFGIKSLIEVYP
>rna-XM_006467653.3
MGDIENQGVTLREMFEGQVHLKKGVIKILSSMSLKCAVELGIADVIHSHGRPITLSELASALNIQPTKTRSLFHFMRLFVHVGLFLKTKVVNHNEQEEAYGLTPTSTLLIKDNSYCLSPLVTGLLDQDFVSSFQYLSSWFKANDLTLWETVRGLKFWDYLNQNPGLSQQFNQVIESDSVMATLLVKDCRPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYIEGDMFQFIPPSDAFFFKTVFHFFDDEDCLKLLKKCREAIASNGERGKVLIIDIVIDEKEDDRELTESKLLFDIFMNFNVGGKERTEQEWGSLFVNAGFTHYKIAPIFGIKSLIEVYP
>rna-XM_025094656.1
MGDIENDGVTVRELFEGQAHLYKGIVKNLSSMSVKCAVELGIADAIHSHGRPITLSELASALNIQPTKTRSLFHFMSLLVHMGMFSKTEVDNHNEQEEAYGLTPTSTLPSKISPTHLSRWFKGNDITLWETFRGLKFWDYLNQNTALTKRFNQAMASDSKMATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLELPHAVTDMPQTENLKYVEGDMFQFIPPADAFFFKTVFHLFDDEDCLKLLKECREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAGFTHYKVAPIFGIKSLIEVYP
>rna-XM_006468285.3
MDPIQNEGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVQSAQQQQEEAYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMFQSIPPADAFFFKAIFHAFVDEDCLKILKRCREAIASRGDRGKVIIIDIVINEKKEDAQLTEAKLLYDMLMMVAVRGSERTEKEWEKLFLDAGFSHFKITPVYGIKSLIEVYP
>rna-XM_006471086.3
MSSIEVEDANELFQAQAHLYKHVLSYMSSIFLKCAVELGIPDIIHKRGRPVTLPELVSALEFQPNKRNCLRRIMRLLDHSGFFSTTKVHNSREEENEAYALTSASKLLLKDKPYCLSPFVLLVTDAAFITPGHYLSRWLRGNELPDPFVTGHGINIWGYAEQNHEFNNLFNQGLASDSQMAKLIIKDCKHIFDGLSSLVEVGGGTGSFARIISEAFPSIKCSVLELPHVIADLPETDNLKFIAGDMNQSIPSADAFLFKLIFHDYDDEVCLKLLKNCREAVASSDGREKVIIVDIVVNEKKDKPEITEAKLLYDALMMTCVPGIERSEKEWERLFFDAGFTSYKITPLLGLRSFIEVYL
>rna-XM_025102642.1
MDANQDQGAKELFQGQAQLYKLMFNHLSSMSLKCAIELGIADIIHSHGRAITLSELVSALDIQPTKTTGLFKLMRLLVHSGCFNKTKVNGQEEAYGLTAASTLLIKDKPYCFSPNVSLFLDPCFVGAFQSLSSWFKGTELTLWETVHGIKFWEFMNQNPGINQRFNEAMASDTEIMTSFVVKAECKQIFEGLGSLVDVGGGNGSLSRIISEAFPGIKCTVLDLPHVVANLPEADNLKYIAGDMFQFIPPADAFLFKLVFHGLDDEDGLKILKKRREAIASNGEGGKVIIIDIVINAEEEEHELTETKFLFDILMSVNANGKERTESEWAKLFSDAGFSHYKITPIFGMRSLIEVYP
>rna-XM_006491981.2
MDTIENEGGISVRELFEGHGQVIRLVFNHLSAMSLKGAVELGIADIIHSHGRPITLSELVSALKIQPTKTSNLFRFMRLLVHMGLFSKTKVGGEKEEEAYGLTAISALLIKDKSYCLSPLVSGILDPENIFPLHFISKWFKGNDISVWETVHGKNYWDYMNQNPGLSQRFNQAMISDSEMAIFIVKDCCRKIFEGLGSIVDVGGGNGGFSKIISEAFPGIKCTVLDLPHAVANMPQTENLKYIAGDMFQYIPPADAYFFKLVFHAFGDEDCLKILKKCREAIAGNGERGKVLIMDIVINEKEDKHQVTEAKLLGDTLMSVSVDGKERTDEEWKTLFLDAGFTHYKITNVFGLKSLIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University