Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Cinnamomum kanehirae
Species name
Species info
Gene sequence info
Cinnamomum_kanehirae.ANR.fa
Download
>rna-gnl|WGS:QPKB|mrna.CKAN_00049000
MGVCVTGAGGYVASWLVKLLLSKGHKVHGTVRDPGDEKNSHLKRLEKAQENLRIFKANLLDYNSLCAATAGCRGVFHIASPVPSSILENPETEVLETAVRGTLNVLKACSETGVERVVIVSSTAAVVNNPNWPPNRPMDEECWSDIDYCRKSRVTAQNVQPNNDQLGSEWIFSNQMQWYNWYCISKTASEREALKYSEKSGLDVVTVCPSRILGPLLQPTVNSSSQLFIELFKGGVETIDDRIWVIVDVRDVAEALLLVYDKKGASGRYICASHPIRVQSMVEKLKSKYPYYSYPKNYIEVDQEVKLSSEKLKRFGWECRSLDETIFDTVEYYREAGGFLASWLVKLLLSKGYMVHGTVRDPGDEKNSHLKRLEKAQENLILFKGNLLDYNSLCAAIVGCSGVFHVASPVPSSLLDNPETELLEAAVKGTLNVLKVCSETGVERVVVVSSTTAVVNNPNWPPNRPMDEDCWSDIDYCRETTLTFQNWYNWYCIAKTTSEREALKYAENTGLDVVTVCASRVLGPLLQRTVNASSQLFSELFKGGVETIDNRVWVIVDVRDVAEALLLVYDKKEASGRYICASHLVRVESVIEMLKSKYPYYNYPKNYIEVDQEVKLSSEKLKRLGWECRSLEDTIVDSVECYQEAADQQPANWDIGSSLLLPLREGEMEEKGRVCVTGAGGYVASWLVKLLLSKGYMVHGTVRDPDDEKNSHLKTLEKAQEKLQLFKANLVDYDSLYAAIEGCSGTELLEPAVKGTLNVLKACSETGVERAVVVSSVGAVMMNPNWPPNRPMDEDCWSDIDYCRESKPLFNWYCISKTTSEREALEYAENTGLDVVTVCPSRVLGPLLQPTVNGSSQLFIDLFKGGAETTDNRLWFIVDVRDVAEALLLVYDKKEASGRYICASHPVRVQSLVEKLKTKYPCYNYPKNYTEVDQELKLSSEKLKRLGWECRSLEDTIVDSVVCYQEAANWDVGSSLLLPLREGEMEEKGRVCVTGAGGYVASWLVKLLLSKGYMVHGTVRDPDDEKNSHLKSLEKAQEKLLLFKANLVDYDSLYAAIEGCSGVFHVATPIPSSSTELLEPTVRGTLNVLKACSETGVERAVVVSSVGAVMMNPNWPPNSPMDEDCWSDIDYCRESKSLFNWYCISKTTSEREALKYAENTGLDVVTVCPSRVLGPLLQPTVNISSQLFIDLFKGGAETTDNRLWFIVDVRDVAEALLLVYDKKEASGRYICASHPVRVQSLVEKLKTKYPCYNYPKNYTEVDQELTLSSEKLKRLGWECRSLEETIVDSVVCYQEAEEGFNYQRLTNNKTIGILGLLPPYERERERERERERRRRNGRNRESVCNRCRRVCGIMVGEAASLKRIYGDEKNNHLKRLEKAQENLQLFKANLLDSDSLYAAFEGCSGVFHVASPVPTSTIELMEPAVRGTLNVLKACSETGVERVVVVSSTSAVVVNPNWPPNRPMDEDCWSDVDYCMESQEYWYNWYCVAKTTAEREALKYAENTGLDVVTVCPSLIFGPLLQTTINTSSQDFIELFKGRLETLKNRIWAIVDVRDVAEALLLVYEKKEASGRYICASHPVQVNSLVEKLKSKYPNYNYPKNYIEVDPEAKPSSEKLKRLGWECRSLEETIDDTVEYYQEAEQQRMPKRDDLFNLNFQKLSVAFGPCVWNEQAKQTGVN
>rna-gnl|WGS:QPKB|mrna.CKAN_00098700
MEGERGRVCVTGAGGFIGSWVVKLLLSEGYMVHGTVREPDDEKNAHLKILDKAKENLQLFKANLLDYDSLLSAIKGCNGVFHVASPVLGNIELIEPAVTGTLNVLKACSVMSIKRVVVVSSIGAVHMNPNWPKDKVMDEDCWSDKEYCKISEEAFNWYSLSKTMAESEALEYAKKNTLEVVTVCPSWVMGPMLQPSINSSSTFLILLLQGKFETIENKNYVLVDVRDVAEALLLVYEKQEASGRYICGAHHFRYRDLIHKLQAMYPNYNYPKNFVEVDEELKLSSEKLMRLGWKYRSLEETLVDTVEYIEETGSVCVTGAGGYLASWLVKLLLSKGYKVHGTVREPDDEKNAHLKKLERAEENLQLFKANLLDYGFLSAAITGCSGVFHVASPVPSGSGLLLLAVDEALTYNYTNAQRIFCGGFFGPASHVTILQVELIEPAVTGTVNVLKACSEAGVKRVVVVSSGAAVAMNPNWPKDKVIDEDCWSDEQYCKTTENWYCASKTIAEREALQYADKNNLDVITVCPALILGPLLQSTANTSSLFLVKLLKGGLETTENKTRAIVDVRDVAEALLLTYEKPEASRRYICASHIIQIRDLVGKLRSMYPNYKYPKNFTDVDGNGNMNMSSEKLKRLGWQCRPLEETLRERGREKKNGRERECMRDGCRRVSSIMASEASPLEGIQDSVNLSDDEKNAHLKKLERAEENLQLFKANLLDYGSLSAAITGCSGVFHVASPVDLIEPAVTGTVNVLKACSEAGVKRVVVVSSGAAILMNPNWPKDKVMDEDCWSDEQYCKTTENWYCASKTIAERAALQYADKNNLDVITVCPTQILGPLLQSTANASSLFLVKLLKGGLETMENKTRVIVDVRDVAEALLLTYEKPEASGRYICAPHIIQIRDLVGKLRSMHPNYKYPKNFTDSAGDVKISSEKLKRLGWQCRPLEETLVNTRERGREKKDGRERECMRDGCRRVSSIMASEASPLEGIQDDEKNAHLKKLERAEENLQLFKANLLDYGSLSAAITGCSGVFHVASPVPSGTLTYNYTYAQRIFCGGFLGPASHITILQVDLIEPAVTGTVNVLKACSEAGVKRVVVVSSGAAILMNPNWPKDKVMDEDCWSDEQYCKTTEQKGRLCSIWIKNNLDVINSLPNPNTWSSAAIHCKRKQLIFGQATERRVRNNGKQNAGELLMCEML
>rna-gnl|WGS:QPKB|mrna.CKAN_01073300
MAEFCVTGGTGFIGAYLVKALLEKGYLVRTTVRDPDDAEKVGFLWSMSGAKERLKVFKADLMVEGSFEDAVDGVDGVFHTASPVLVPYDSNIQASNMTHFNERPMALFGSSVCIHQFTYNRLTQNKTANVLKLQENLIDPCVEGTKNVLKSCAKASSVKRVVLTSSSSAIRYREDMTQASPLNESHWSDPDYCKRYNLWYPYAKTLAEKAAWQLAEETGIDLVVVNPTFIVGPLITPQPTSTLRMVLSYMKGEASVYPNFTVGFVHIEDVIACHIVAMEDSRVTGRLICSSTVAHWSEIIEMLKATYPSYPITDKCGQQIGDNNPHRMDTSKIKQLGFSNFKTLAQMFDDCIKSFQVKGLL
>rna-gnl|WGS:QPKB|mrna.CKAN_01442200
MPEYCVTGGAGFIASYLIKALLENGCLVRTTVRNPDDAEKVGFLWSMSGAKDRLKVMKADLLVEGSFDMAVDGVDGVFHTAAPVLVPYDENVQASKNTV
>rna-gnl|WGS:QPKB|mrna.CKAN_02010300
MATQTAVKKLACVTGGSGFVAALLVKRLLEKGYAVNTTVRDPANKTKVSHLVDLQGLGELKLYQADLTDEGSFDEAISGCDIVFHVATPVHFESKDPENDMIKPAIQGTLNVLKSCMKAKTVKRVVLTSSAAAVSLNQLTGTGHVVDEECWSDVEFLASEKPPTWGYPVSKTLAEKAAWKFAEENKLDLITVIPVLMAGHSLTKDVPSSTSLAMALLTGNEFLINGLKGMQLLSGSISMAHVDDVCQAHIFLAENESASGRYICCQINTCVTELAKFLSERYPQYKVPTDFGGFPATPKLSISSEKLIKAGFTFKYGIEEIYDETVEYFKSVGLLAE
>rna-gnl|WGS:QPKB|mrna.CKAN_02241700
MEKNICVCVTGAAGYIASWLVKKLLDKGYTVHATLRNLENPSKVGLLKALPHADTRLVLFGADIYKAEEFEPAIRGCQFVFHVATPLLHNTHSTKYKDTTEAAIEGVKSILGSCIRSKTVERLIYTGSVVATSPLKEDGSSFKDLIDESCWTPLHHSFSYSSFIDQEYISSKTQSEKEVLSYCNMEEGGLEVVSLACALVGGDTLLSYQPTSVGVIISQLTGEEDHYRRLKLLQEMLGSVPLIHIDDVCEAHIFCMEQPSMAGRFLCACTYPTIAEMAKHFTNKYPEIQVAKRFSEGPETGIDCQSTKLRDMGFKYNYDFKRILDDSVECSKRMAKGKKKKPQHLKAQEQLSNRAQRKTAAEQRKPPCKTELSTHSAAEIRKQPAAEKQKERTKPNRAQQTTAQTEPSSQKQNTHQKQQRDAMNCLKEIRPDAAVTPIS
>rna-gnl|WGS:QPKB|mrna.CKAN_02241900
MEKNMCVCVTGAAGFIGSWLVKKLLDKGYTVHATLRNLEDSSKVGLLKRLPHADTSLVLFRADIYNAEEFEPAIRGCAFVFHVATPMLHNNPTSNSKFKDTTEAAIAGVRSILGSCLRSGTVKRLIYTGSIMAASPLKEDGSNFKDSIDESCWTPLHHSFSYSSDLEQAYISSKTLSEKEVLSYCNIEDGGLEVVSLPCALVGGDTHLSHVPLSIEVVISQLTGKEVQYRQLKLLEEMLGSVPLIHIDDICEAHIFCLEQPSMAGRFLCASAYPTIAEMAKYFSDKYPEIQIAKEFAGPEIRIDCGSTKLTDMGFQFNYDLKRILDDSVQCARRLGLLK
>rna-gnl|WGS:QPKB|mrna.CKAN_02241800
MEKKICVCVTGAAGYLGSWLVKKLLDKGYTVHATLRSLEDPSKVGLLKALPHADTRLVLFRADIYEPEEFEPAIKGCEFVFHVATPFLHNTHSTKFKDTTEAAIAGVKSIVDSCIRSRTVKRLIYTGSVMAASPLKECASVFMDSIDEGCWTPLHHSFSYSSIFEQAYTSSKTLCEKEVLSYCNIEERGLEVVSLTCALVGGDTLLSHLPLSVEVVISQLTGEEARFRQLKLLQEMLGSVPLIHIDDVCEAHVFCVEQPSMAGRFLCACVCPTIAEMAKYFSDNYPKIQTAKE
MGVCVTGAGGYVASWLVKLLLSKGHKVHGTVRDPGDEKNSHLKRLEKAQENLRIFKANLLDYNSLCAATAGCRGVFHIASPVPSSILENPETEVLETAVRGTLNVLKACSETGVERVVIVSSTAAVVNNPNWPPNRPMDEECWSDIDYCRKSRVTAQNVQPNNDQLGSEWIFSNQMQWYNWYCISKTASEREALKYSEKSGLDVVTVCPSRILGPLLQPTVNSSSQLFIELFKGGVETIDDRIWVIVDVRDVAEALLLVYDKKGASGRYICASHPIRVQSMVEKLKSKYPYYSYPKNYIEVDQEVKLSSEKLKRFGWECRSLDETIFDTVEYYREAGGFLASWLVKLLLSKGYMVHGTVRDPGDEKNSHLKRLEKAQENLILFKGNLLDYNSLCAAIVGCSGVFHVASPVPSSLLDNPETELLEAAVKGTLNVLKVCSETGVERVVVVSSTTAVVNNPNWPPNRPMDEDCWSDIDYCRETTLTFQNWYNWYCIAKTTSEREALKYAENTGLDVVTVCASRVLGPLLQRTVNASSQLFSELFKGGVETIDNRVWVIVDVRDVAEALLLVYDKKEASGRYICASHLVRVESVIEMLKSKYPYYNYPKNYIEVDQEVKLSSEKLKRLGWECRSLEDTIVDSVECYQEAADQQPANWDIGSSLLLPLREGEMEEKGRVCVTGAGGYVASWLVKLLLSKGYMVHGTVRDPDDEKNSHLKTLEKAQEKLQLFKANLVDYDSLYAAIEGCSGTELLEPAVKGTLNVLKACSETGVERAVVVSSVGAVMMNPNWPPNRPMDEDCWSDIDYCRESKPLFNWYCISKTTSEREALEYAENTGLDVVTVCPSRVLGPLLQPTVNGSSQLFIDLFKGGAETTDNRLWFIVDVRDVAEALLLVYDKKEASGRYICASHPVRVQSLVEKLKTKYPCYNYPKNYTEVDQELKLSSEKLKRLGWECRSLEDTIVDSVVCYQEAANWDVGSSLLLPLREGEMEEKGRVCVTGAGGYVASWLVKLLLSKGYMVHGTVRDPDDEKNSHLKSLEKAQEKLLLFKANLVDYDSLYAAIEGCSGVFHVATPIPSSSTELLEPTVRGTLNVLKACSETGVERAVVVSSVGAVMMNPNWPPNSPMDEDCWSDIDYCRESKSLFNWYCISKTTSEREALKYAENTGLDVVTVCPSRVLGPLLQPTVNISSQLFIDLFKGGAETTDNRLWFIVDVRDVAEALLLVYDKKEASGRYICASHPVRVQSLVEKLKTKYPCYNYPKNYTEVDQELTLSSEKLKRLGWECRSLEETIVDSVVCYQEAEEGFNYQRLTNNKTIGILGLLPPYERERERERERERRRRNGRNRESVCNRCRRVCGIMVGEAASLKRIYGDEKNNHLKRLEKAQENLQLFKANLLDSDSLYAAFEGCSGVFHVASPVPTSTIELMEPAVRGTLNVLKACSETGVERVVVVSSTSAVVVNPNWPPNRPMDEDCWSDVDYCMESQEYWYNWYCVAKTTAEREALKYAENTGLDVVTVCPSLIFGPLLQTTINTSSQDFIELFKGRLETLKNRIWAIVDVRDVAEALLLVYEKKEASGRYICASHPVQVNSLVEKLKSKYPNYNYPKNYIEVDPEAKPSSEKLKRLGWECRSLEETIDDTVEYYQEAEQQRMPKRDDLFNLNFQKLSVAFGPCVWNEQAKQTGVN
>rna-gnl|WGS:QPKB|mrna.CKAN_00098700
MEGERGRVCVTGAGGFIGSWVVKLLLSEGYMVHGTVREPDDEKNAHLKILDKAKENLQLFKANLLDYDSLLSAIKGCNGVFHVASPVLGNIELIEPAVTGTLNVLKACSVMSIKRVVVVSSIGAVHMNPNWPKDKVMDEDCWSDKEYCKISEEAFNWYSLSKTMAESEALEYAKKNTLEVVTVCPSWVMGPMLQPSINSSSTFLILLLQGKFETIENKNYVLVDVRDVAEALLLVYEKQEASGRYICGAHHFRYRDLIHKLQAMYPNYNYPKNFVEVDEELKLSSEKLMRLGWKYRSLEETLVDTVEYIEETGSVCVTGAGGYLASWLVKLLLSKGYKVHGTVREPDDEKNAHLKKLERAEENLQLFKANLLDYGFLSAAITGCSGVFHVASPVPSGSGLLLLAVDEALTYNYTNAQRIFCGGFFGPASHVTILQVELIEPAVTGTVNVLKACSEAGVKRVVVVSSGAAVAMNPNWPKDKVIDEDCWSDEQYCKTTENWYCASKTIAEREALQYADKNNLDVITVCPALILGPLLQSTANTSSLFLVKLLKGGLETTENKTRAIVDVRDVAEALLLTYEKPEASRRYICASHIIQIRDLVGKLRSMYPNYKYPKNFTDVDGNGNMNMSSEKLKRLGWQCRPLEETLRERGREKKNGRERECMRDGCRRVSSIMASEASPLEGIQDSVNLSDDEKNAHLKKLERAEENLQLFKANLLDYGSLSAAITGCSGVFHVASPVDLIEPAVTGTVNVLKACSEAGVKRVVVVSSGAAILMNPNWPKDKVMDEDCWSDEQYCKTTENWYCASKTIAERAALQYADKNNLDVITVCPTQILGPLLQSTANASSLFLVKLLKGGLETMENKTRVIVDVRDVAEALLLTYEKPEASGRYICAPHIIQIRDLVGKLRSMHPNYKYPKNFTDSAGDVKISSEKLKRLGWQCRPLEETLVNTRERGREKKDGRERECMRDGCRRVSSIMASEASPLEGIQDDEKNAHLKKLERAEENLQLFKANLLDYGSLSAAITGCSGVFHVASPVPSGTLTYNYTYAQRIFCGGFLGPASHITILQVDLIEPAVTGTVNVLKACSEAGVKRVVVVSSGAAILMNPNWPKDKVMDEDCWSDEQYCKTTEQKGRLCSIWIKNNLDVINSLPNPNTWSSAAIHCKRKQLIFGQATERRVRNNGKQNAGELLMCEML
>rna-gnl|WGS:QPKB|mrna.CKAN_01073300
MAEFCVTGGTGFIGAYLVKALLEKGYLVRTTVRDPDDAEKVGFLWSMSGAKERLKVFKADLMVEGSFEDAVDGVDGVFHTASPVLVPYDSNIQASNMTHFNERPMALFGSSVCIHQFTYNRLTQNKTANVLKLQENLIDPCVEGTKNVLKSCAKASSVKRVVLTSSSSAIRYREDMTQASPLNESHWSDPDYCKRYNLWYPYAKTLAEKAAWQLAEETGIDLVVVNPTFIVGPLITPQPTSTLRMVLSYMKGEASVYPNFTVGFVHIEDVIACHIVAMEDSRVTGRLICSSTVAHWSEIIEMLKATYPSYPITDKCGQQIGDNNPHRMDTSKIKQLGFSNFKTLAQMFDDCIKSFQVKGLL
>rna-gnl|WGS:QPKB|mrna.CKAN_01442200
MPEYCVTGGAGFIASYLIKALLENGCLVRTTVRNPDDAEKVGFLWSMSGAKDRLKVMKADLLVEGSFDMAVDGVDGVFHTAAPVLVPYDENVQASKNTV
>rna-gnl|WGS:QPKB|mrna.CKAN_02010300
MATQTAVKKLACVTGGSGFVAALLVKRLLEKGYAVNTTVRDPANKTKVSHLVDLQGLGELKLYQADLTDEGSFDEAISGCDIVFHVATPVHFESKDPENDMIKPAIQGTLNVLKSCMKAKTVKRVVLTSSAAAVSLNQLTGTGHVVDEECWSDVEFLASEKPPTWGYPVSKTLAEKAAWKFAEENKLDLITVIPVLMAGHSLTKDVPSSTSLAMALLTGNEFLINGLKGMQLLSGSISMAHVDDVCQAHIFLAENESASGRYICCQINTCVTELAKFLSERYPQYKVPTDFGGFPATPKLSISSEKLIKAGFTFKYGIEEIYDETVEYFKSVGLLAE
>rna-gnl|WGS:QPKB|mrna.CKAN_02241700
MEKNICVCVTGAAGYIASWLVKKLLDKGYTVHATLRNLENPSKVGLLKALPHADTRLVLFGADIYKAEEFEPAIRGCQFVFHVATPLLHNTHSTKYKDTTEAAIEGVKSILGSCIRSKTVERLIYTGSVVATSPLKEDGSSFKDLIDESCWTPLHHSFSYSSFIDQEYISSKTQSEKEVLSYCNMEEGGLEVVSLACALVGGDTLLSYQPTSVGVIISQLTGEEDHYRRLKLLQEMLGSVPLIHIDDVCEAHIFCMEQPSMAGRFLCACTYPTIAEMAKHFTNKYPEIQVAKRFSEGPETGIDCQSTKLRDMGFKYNYDFKRILDDSVECSKRMAKGKKKKPQHLKAQEQLSNRAQRKTAAEQRKPPCKTELSTHSAAEIRKQPAAEKQKERTKPNRAQQTTAQTEPSSQKQNTHQKQQRDAMNCLKEIRPDAAVTPIS
>rna-gnl|WGS:QPKB|mrna.CKAN_02241900
MEKNMCVCVTGAAGFIGSWLVKKLLDKGYTVHATLRNLEDSSKVGLLKRLPHADTSLVLFRADIYNAEEFEPAIRGCAFVFHVATPMLHNNPTSNSKFKDTTEAAIAGVRSILGSCLRSGTVKRLIYTGSIMAASPLKEDGSNFKDSIDESCWTPLHHSFSYSSDLEQAYISSKTLSEKEVLSYCNIEDGGLEVVSLPCALVGGDTHLSHVPLSIEVVISQLTGKEVQYRQLKLLEEMLGSVPLIHIDDICEAHIFCLEQPSMAGRFLCASAYPTIAEMAKYFSDKYPEIQIAKEFAGPEIRIDCGSTKLTDMGFQFNYDLKRILDDSVQCARRLGLLK
>rna-gnl|WGS:QPKB|mrna.CKAN_02241800
MEKKICVCVTGAAGYLGSWLVKKLLDKGYTVHATLRSLEDPSKVGLLKALPHADTRLVLFRADIYEPEEFEPAIKGCEFVFHVATPFLHNTHSTKFKDTTEAAIAGVKSIVDSCIRSRTVKRLIYTGSVMAASPLKECASVFMDSIDEGCWTPLHHSFSYSSIFEQAYTSSKTLCEKEVLSYCNIEERGLEVVSLTCALVGGDTLLSHLPLSVEVVISQLTGEEARFRQLKLLQEMLGSVPLIHIDDVCEAHVFCVEQPSMAGRFLCACVCPTIAEMAKYFSDNYPKIQTAKE
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University