Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Chamaecrista fasciculata
Species name
Golden cassia (Chamaecrista fasciculata) is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Chamaecrista_fasciculata.FLS.fa
Download
>Chafa706S26028
MEEKRVQTLASSQLKKLPPQFIRSANERPENTKAVEGVKMPVISLFSNSKPRNLVVQEISRASCDWGFFFIIDHGISPTLIQRLQEVGQEFFALPQKEKEAYANDPTKGVFEGYGTNMTYNPENKVEWIDYYFHLLSPPSKVNYNKWPQSPPSYRQVTEEYSKEIQRVTEEVLELLSEGLRLEKKTLQSSLGNEEIELEMKINMYPPCPQPELALGVEPHTDMSALTLLVPNQIPGLQVWKNNEWVAVNHMQNALIVLVGDQLEVLSNGKYKSVVHRSLVNKEHLRMSWAVFAVPPHEVVIGPLPDLVNHQNPPKFPTKTFAKYRYLKFNKLPQ
>Chafa11714S14534
MELPVIDLSPYLAVSGDPSSDLAAICSEVSRNLTETGALLIKDPRCSVEDNDRFIDMMERYFQSPHDFKRRQERPHLHYQVGVTPEGVEVPRSLVDVEMQEKLRAMPKEHQPLTPTGPDVKWRYFWRIGPRPSNTCFQELNSEPVIPEGFPEWKETMDSWGYKMISAIEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLQKYGQEGTVFAGYHYDLNFLTIHGRSRFPGLNIWLRNGQKVEVKVPVGCLLIQAGKQIEWLTAGHCIAGMHEVVATNRTLDAIKLAKEQNRSLWRVSSTLFAHIASDAVLKPLGHFAELPLASKYPPMRAGEYVEQELAVINLKGKK
>Chafa95578S30083
MDAIRVENVACMSKESNNIPSEFIRPETEQPGITTVQGVNLEVPTIDFNQQEDQVLMDQIMEASRDWGMYQIVNHGIPSDVIANLQAVGKAFFDLPQQQKELIAKLPGSVEGYGTLLQKDIQIEGKKGWVDHLFHRVWPPSAINYRFWPTHPPSYREANEEYVKQLHGVIDKLLKSIGKGIGMEGHEVRDALGGEKLEYLMKINYYPPCPRPDLVLGVPAHTDMSAITILVPNHVQGLQAYRDGHWYDVKYIPNALVIHIGDQLEILSNGKYKAVLHRTTVCDETRMSWPLFLEPPANQMVGPHPKLIDDQNPPKFKTKTFADFAYCKFNKISQ
>Chafa6096S24997
MDATRVENVACMSKKSNNIPLEFIRPETEQPGITTVRGMKLEVPTIDFSQQQDEVLMDQIMEASRDWGMYQIVNHGIPNDVIANLQAVGKAFFDLPQQQKEIIAKLPGSVEGYGTLLQKDIQIEGKKGWVDHLFHRVWPPSTINYRFWPTHPPSYREANEEYVKQLHGVIDKLLKSIGKGIGLEGHEVRDALGGEKLEYLMKINYYPPCPRPDLALGVPAHTDMSAITILVPNHVQGLQAYRDGHWYDVKYIPNALVIHIGDQLEILSNGKYKAVLHRTTVCDETRMSWPLFLEPPANQMVGPHPKLIDDQNPPKFKXML
>Chafa9475S28455
MDAIRVENVACMSKESNNIPSEFIRPETEQPGITTVQGVNLEVPTIDFNQQEDQVLMDQNMEARMYQIVNHGIPSDVIANLQVVGKAFFDLPQQQKELIAKLPGSVEGYGTLLQKDIQIEGKKGWVDHLFHRVWPPSAINYRFWPTHPPSYREANEEYVKQLHGVIDKLLKSIGKGIGMEGHEVRDALGGEKLEYLMKINYYPPCPRPDLALGVPAHTDMSAITILVPNHVQGLQAYRDGHWYDVKYIPNALVIHIGDQLECKAVLHRTTVCDETKMSWPLFLEPPANQMVGPHPKLIDDQNPPKFKTKTFADFAYCKFNKIPQ
>Chafa11063S14092
MCITNNKEVLDINLCVHRQVTEEYHKEILRVTEEVLKLLSEGLGLEKKTLKRSLGNENKYVSTIPTTRTSIWIPNDIAGLQFWKNNDWVAVNHLQNTLIVLVGDQLEVLSNGKYKNVVHRSLVNKEHLRMSWAVFVVPPHELGEPSKVQNPPKFPTKAFAEYRYRKFNKLP
MEEKRVQTLASSQLKKLPPQFIRSANERPENTKAVEGVKMPVISLFSNSKPRNLVVQEISRASCDWGFFFIIDHGISPTLIQRLQEVGQEFFALPQKEKEAYANDPTKGVFEGYGTNMTYNPENKVEWIDYYFHLLSPPSKVNYNKWPQSPPSYRQVTEEYSKEIQRVTEEVLELLSEGLRLEKKTLQSSLGNEEIELEMKINMYPPCPQPELALGVEPHTDMSALTLLVPNQIPGLQVWKNNEWVAVNHMQNALIVLVGDQLEVLSNGKYKSVVHRSLVNKEHLRMSWAVFAVPPHEVVIGPLPDLVNHQNPPKFPTKTFAKYRYLKFNKLPQ
>Chafa11714S14534
MELPVIDLSPYLAVSGDPSSDLAAICSEVSRNLTETGALLIKDPRCSVEDNDRFIDMMERYFQSPHDFKRRQERPHLHYQVGVTPEGVEVPRSLVDVEMQEKLRAMPKEHQPLTPTGPDVKWRYFWRIGPRPSNTCFQELNSEPVIPEGFPEWKETMDSWGYKMISAIEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLQKYGQEGTVFAGYHYDLNFLTIHGRSRFPGLNIWLRNGQKVEVKVPVGCLLIQAGKQIEWLTAGHCIAGMHEVVATNRTLDAIKLAKEQNRSLWRVSSTLFAHIASDAVLKPLGHFAELPLASKYPPMRAGEYVEQELAVINLKGKK
>Chafa95578S30083
MDAIRVENVACMSKESNNIPSEFIRPETEQPGITTVQGVNLEVPTIDFNQQEDQVLMDQIMEASRDWGMYQIVNHGIPSDVIANLQAVGKAFFDLPQQQKELIAKLPGSVEGYGTLLQKDIQIEGKKGWVDHLFHRVWPPSAINYRFWPTHPPSYREANEEYVKQLHGVIDKLLKSIGKGIGMEGHEVRDALGGEKLEYLMKINYYPPCPRPDLVLGVPAHTDMSAITILVPNHVQGLQAYRDGHWYDVKYIPNALVIHIGDQLEILSNGKYKAVLHRTTVCDETRMSWPLFLEPPANQMVGPHPKLIDDQNPPKFKTKTFADFAYCKFNKISQ
>Chafa6096S24997
MDATRVENVACMSKKSNNIPLEFIRPETEQPGITTVRGMKLEVPTIDFSQQQDEVLMDQIMEASRDWGMYQIVNHGIPNDVIANLQAVGKAFFDLPQQQKEIIAKLPGSVEGYGTLLQKDIQIEGKKGWVDHLFHRVWPPSTINYRFWPTHPPSYREANEEYVKQLHGVIDKLLKSIGKGIGLEGHEVRDALGGEKLEYLMKINYYPPCPRPDLALGVPAHTDMSAITILVPNHVQGLQAYRDGHWYDVKYIPNALVIHIGDQLEILSNGKYKAVLHRTTVCDETRMSWPLFLEPPANQMVGPHPKLIDDQNPPKFKXML
>Chafa9475S28455
MDAIRVENVACMSKESNNIPSEFIRPETEQPGITTVQGVNLEVPTIDFNQQEDQVLMDQNMEARMYQIVNHGIPSDVIANLQVVGKAFFDLPQQQKELIAKLPGSVEGYGTLLQKDIQIEGKKGWVDHLFHRVWPPSAINYRFWPTHPPSYREANEEYVKQLHGVIDKLLKSIGKGIGMEGHEVRDALGGEKLEYLMKINYYPPCPRPDLALGVPAHTDMSAITILVPNHVQGLQAYRDGHWYDVKYIPNALVIHIGDQLECKAVLHRTTVCDETKMSWPLFLEPPANQMVGPHPKLIDDQNPPKFKTKTFADFAYCKFNKIPQ
>Chafa11063S14092
MCITNNKEVLDINLCVHRQVTEEYHKEILRVTEEVLKLLSEGLGLEKKTLKRSLGNENKYVSTIPTTRTSIWIPNDIAGLQFWKNNDWVAVNHLQNTLIVLVGDQLEVLSNGKYKNVVHRSLVNKEHLRMSWAVFVVPPHELGEPSKVQNPPKFPTKAFAEYRYRKFNKLP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University