Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Chamaecrista fasciculata
Species name
Golden cassia (Chamaecrista fasciculata) is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Chamaecrista_fasciculata.HI4_OMT.fa
Download
>Chafa6170S25063
MGSSPEETNPSSSEMMFEAQAHLYKHLFSCLNGMVLKCAIQLNIPDIIHNHGKPITIPELASKLQVHPTKTTYVGRLVRLLSHNNFLAESKSLNKDITKESSYAYTLTPSFKLLIKGHDPCLVPMVNCFLDSTVMAPFDVLDEWFKGDKKQASECVFGVGWWDVLERDKKYMSLFNEAMASDSRLIDVALREKCEWVFKGLDSLVDVGGGTGTTARIISEAYPNMKCMVFDLPHVVQNLKGDENLSFVGGSMFDDSIPFADAVLLKWVLHDWDDESCIKILKKCKEAISNSHNGTKGKVIIIDAVINEKMEEHAMTELKLLLDVLMLAINGKERNVQEWDILLRSAGFKHYNITPLFGFRSIIEAFP
>Chafa6170S25062
MASLEENKPSSSEIMFEAQAHLYKHLFGCLNGMVLKCAIHLNIPDIIHNHGKPITIPELASKLQVHPTKTAYVGRLVRLLAHNGFLLAESQKALKVMSDKNNKEIMNSESYTHTYTLTPSFKLLIKGHDPCLVPMVIGILGPNFISPYDVLHKWFKGDEKTAAETAFGMTLWDFLKGDTECMNSFNEAMASDSQMINMALRECEWVFKGLDSLVDVGGGTGTTARIISEVYPDLKCMVFDFPHVVQNLKGDENLSFVGGYMFDDSIPSLLLMLFFLRVRRETLTVAKVAEIIKPSSSKIMFAAEAHLYKHLFGCLNGSVLKCAIHLNIPDIIHNHGKPITIPELASKLQVHPTKISYVDRLVRLLTHNGFLAESKSLKLVMCDKNNEEINKESYTYTLTPSFELLIKGHDPCLVPMVNCFLDSTVMAPFDVLDEWFKGDKKQASECVFGVGWWDVLERDAKYMSLFNEAMASDSQMINMALRDCEWVFKGLNSLVDVGGGTGTTARIISQAYPHMKCVVFDLPKVVQNLKGDNEHLSYVGGNMFDDCIPSADAVLLKAILIWILHDWNNESCLKILKKCKEAISNSKGRGKVIIIDAVINEKMDEHAMTELKLLLDVLMLAINGKERNVQEWDILLRSAGFKHYNITPLFGFRSIIEAYP
>Chafa4507S32004
MALTMDMLMMANTNSGKERTIPEWEYVIKQAGFTTFTITHINSLKLLPVWLIGNRDTKIRKRECESQNNK
>Chafa5114S23787
MGSLEKTKPSNSEIMFEAQAHLYKHIFGYLNGMVLKCAIHLNIPDIIHNHGKPITIPELASKLQVHPTKTAYVGRLVHLLAHNGFLAESKSLKLVMCDKNNEEIMKESYIYTYTLTPSSMLLVKGKEPCLVPMVMGLLVPSMMCPFDVLHKWFKGDEKTVSETAYGVGLWDFLQGHAEYMSLFNEAMASDSQMINMALRDCDWVFKGLDSLIDVGGGTGTTARIISQAYPHMKCVVFDLPQVVQNLKGDNEHLNFVGGSMFDDVIPFADAVLLKWILHTWNDESCIKILEKCREAISSSNNEKGKVIIIDAVINEKNNKDHDEDNAMTEVKLLYDVLMLASDGKERNVQEWDILLKAAGFKHYKITPLFGFRSIIEAYP
MGSSPEETNPSSSEMMFEAQAHLYKHLFSCLNGMVLKCAIQLNIPDIIHNHGKPITIPELASKLQVHPTKTTYVGRLVRLLSHNNFLAESKSLNKDITKESSYAYTLTPSFKLLIKGHDPCLVPMVNCFLDSTVMAPFDVLDEWFKGDKKQASECVFGVGWWDVLERDKKYMSLFNEAMASDSRLIDVALREKCEWVFKGLDSLVDVGGGTGTTARIISEAYPNMKCMVFDLPHVVQNLKGDENLSFVGGSMFDDSIPFADAVLLKWVLHDWDDESCIKILKKCKEAISNSHNGTKGKVIIIDAVINEKMEEHAMTELKLLLDVLMLAINGKERNVQEWDILLRSAGFKHYNITPLFGFRSIIEAFP
>Chafa6170S25062
MASLEENKPSSSEIMFEAQAHLYKHLFGCLNGMVLKCAIHLNIPDIIHNHGKPITIPELASKLQVHPTKTAYVGRLVRLLAHNGFLLAESQKALKVMSDKNNKEIMNSESYTHTYTLTPSFKLLIKGHDPCLVPMVIGILGPNFISPYDVLHKWFKGDEKTAAETAFGMTLWDFLKGDTECMNSFNEAMASDSQMINMALRECEWVFKGLDSLVDVGGGTGTTARIISEVYPDLKCMVFDFPHVVQNLKGDENLSFVGGYMFDDSIPSLLLMLFFLRVRRETLTVAKVAEIIKPSSSKIMFAAEAHLYKHLFGCLNGSVLKCAIHLNIPDIIHNHGKPITIPELASKLQVHPTKISYVDRLVRLLTHNGFLAESKSLKLVMCDKNNEEINKESYTYTLTPSFELLIKGHDPCLVPMVNCFLDSTVMAPFDVLDEWFKGDKKQASECVFGVGWWDVLERDAKYMSLFNEAMASDSQMINMALRDCEWVFKGLNSLVDVGGGTGTTARIISQAYPHMKCVVFDLPKVVQNLKGDNEHLSYVGGNMFDDCIPSADAVLLKAILIWILHDWNNESCLKILKKCKEAISNSKGRGKVIIIDAVINEKMDEHAMTELKLLLDVLMLAINGKERNVQEWDILLRSAGFKHYNITPLFGFRSIIEAYP
>Chafa4507S32004
MALTMDMLMMANTNSGKERTIPEWEYVIKQAGFTTFTITHINSLKLLPVWLIGNRDTKIRKRECESQNNK
>Chafa5114S23787
MGSLEKTKPSNSEIMFEAQAHLYKHIFGYLNGMVLKCAIHLNIPDIIHNHGKPITIPELASKLQVHPTKTAYVGRLVHLLAHNGFLAESKSLKLVMCDKNNEEIMKESYIYTYTLTPSSMLLVKGKEPCLVPMVMGLLVPSMMCPFDVLHKWFKGDEKTVSETAYGVGLWDFLQGHAEYMSLFNEAMASDSQMINMALRDCDWVFKGLDSLIDVGGGTGTTARIISQAYPHMKCVVFDLPQVVQNLKGDNEHLNFVGGSMFDDVIPFADAVLLKWILHTWNDESCIKILEKCREAISSSNNEKGKVIIIDAVINEKNNKDHDEDNAMTEVKLLYDVLMLASDGKERNVQEWDILLKAAGFKHYKITPLFGFRSIIEAYP
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