Enzyme
Requires Fe2+ and ascorbate. This plant enzyme catalyses an early step in the flavonoid biosynthesis pathway, leading to the production of flavanols and anthocyanins. Substrates include (2S)-naringenin, (2S)-eriodictyol, (2S)-dihydrotricetin and (2S)-pinocembrin. Some enzymes are bifuctional and also catalyse EC 1.14.20.6, flavonol synthase.
F3H
Enzyme
Flavanone 3-hydroxylase
Enzyme name
EC:1.14.11.9
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Class
(2S)-flavan-4-one,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Sysname
a (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2 [RN:R07329]
Reaction
(2R,3R)-dihydroflavonol [CPD:C21417];succinate [CPD:C00042];CO2 [CPD:C00011]
Product
Species information
Chamaecrista fasciculata
Species name
Golden cassia (Chamaecrista fasciculata) is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Chamaecrista_fasciculata.F3H.fa
Download
>Chafa5069S23719
MASSRPTTLTDVAKETILRSSFVRQEDERPKVAYNQFSDAIPVISLAGMDEVDGRRGEICKKIVEACEEWGIFQIVDHGVDTKLVSQMTRLAKHFFDLSPEDKIRYDMSVSTSFLIMASSRPTTLSDVANETILRSSFVRQEDERPKVSYNDFSDAIPVISLAGIDEVDGRRGEICKKIVEACEEWGIFQIVDHGVDTKLVSEMTRLAKHFFELSPQDKTRYDMSGAKKGGFIGEAVLDWREMFIYFSQPEKSRDYSMWPEKPEGWRKVTEEYSEKLMNLACKLLEILSEAMGLEKEALTKACVEMDQKLVLNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGNTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSKQSRLSIATFQNPSPDAIVYPLKVREGDEAILKDPITFSEMYRRKMVKDLELANLKKLDKERMINSDHANKEKLNFSTKPLDQILD
>Chafa95212S12561
MASSRPTTLTDVAKETILRSSFVRQEDERPKVAYDNFSDAIPVISLAGIDEVDGRRGEICKKIVEACEEWGIFQIVDHGVDTKLLSQMTCLAKHFFHLSPEDKTRYDMSGGKKGGFIGEAVLDWREMFIYLSQPEKSRDYSMWPEKPEGWRKVTEEYSEKLMNLACKLLEVLSEAMGLDKEALTKACVEMDQKLVLNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGNTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSKQSRMSIATFQNPSPDAIVYPLKVREGEEVILNEPITFSEMYRRKMAKDLELANLKKLNKECMNNSDHAPKEKLNFSTKPLDQILA
>Chafa16529S07212
MPTASSAPTTLTAIAEETTLRSRFVRDEDERPKVAYDNFSDAIPVISLAGIDEVDGRRGEICKKIVEACEEWGIFQIVDHGVDTKLLSQMTCLAKHFFDLSPEDKTRYDMSGGKKGGFIVSSHLKGEAVMDWREIFIYFSQPEKSRDYSRWPEKPEGWRKVTEEYSEKLMNLACKLLEVLSEAMGLEKEALTKACIELDQKLVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGNTWITVQPVEGAFIVNLGDHAHYLSNGRFKNADHQAVVNSKQSRLSIATFQNPSQNAIVYPLKVREGEEAILKEPITFSEMYRRKMAKDIELANLKKLKNDRLVKSDHTQQEKHSTKPLDQILA
>Chafa1916S17672
MAPPPPPPKTLTSLSDTNTFESRFERDEDERPKVPYNVFSDDIPVISLAGILDESRRPEIRRKIVEACEEWGIFQVVDHGVDLDLISEMTTLSRNFFALSPEEKLRYDMSGGKKGGFIVSSHLQGEAVMDWREMVTYFSYPMEKRDYSRWPEKPEAWKEVTKEYSEKLMGLACKLLEILSEAMGLDKEALTKSCLEMDQKVVVNYYPKCPKPEVTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKSWITVQPVHGAFVVNLGDHAHYLSNGRFKNADHQAVVNSEQSRLSIATFQNPAPEATVYPLNISDGEKSVMEEAITYAEMYRRKMSGDIELAKLKKLAKEKAMGCNSHDLQKPKLDAKPIDQIFA
MASSRPTTLTDVAKETILRSSFVRQEDERPKVAYNQFSDAIPVISLAGMDEVDGRRGEICKKIVEACEEWGIFQIVDHGVDTKLVSQMTRLAKHFFDLSPEDKIRYDMSVSTSFLIMASSRPTTLSDVANETILRSSFVRQEDERPKVSYNDFSDAIPVISLAGIDEVDGRRGEICKKIVEACEEWGIFQIVDHGVDTKLVSEMTRLAKHFFELSPQDKTRYDMSGAKKGGFIGEAVLDWREMFIYFSQPEKSRDYSMWPEKPEGWRKVTEEYSEKLMNLACKLLEILSEAMGLEKEALTKACVEMDQKLVLNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGNTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSKQSRLSIATFQNPSPDAIVYPLKVREGDEAILKDPITFSEMYRRKMVKDLELANLKKLDKERMINSDHANKEKLNFSTKPLDQILD
>Chafa95212S12561
MASSRPTTLTDVAKETILRSSFVRQEDERPKVAYDNFSDAIPVISLAGIDEVDGRRGEICKKIVEACEEWGIFQIVDHGVDTKLLSQMTCLAKHFFHLSPEDKTRYDMSGGKKGGFIGEAVLDWREMFIYLSQPEKSRDYSMWPEKPEGWRKVTEEYSEKLMNLACKLLEVLSEAMGLDKEALTKACVEMDQKLVLNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGNTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSKQSRMSIATFQNPSPDAIVYPLKVREGEEVILNEPITFSEMYRRKMAKDLELANLKKLNKECMNNSDHAPKEKLNFSTKPLDQILA
>Chafa16529S07212
MPTASSAPTTLTAIAEETTLRSRFVRDEDERPKVAYDNFSDAIPVISLAGIDEVDGRRGEICKKIVEACEEWGIFQIVDHGVDTKLLSQMTCLAKHFFDLSPEDKTRYDMSGGKKGGFIVSSHLKGEAVMDWREIFIYFSQPEKSRDYSRWPEKPEGWRKVTEEYSEKLMNLACKLLEVLSEAMGLEKEALTKACIELDQKLVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGNTWITVQPVEGAFIVNLGDHAHYLSNGRFKNADHQAVVNSKQSRLSIATFQNPSQNAIVYPLKVREGEEAILKEPITFSEMYRRKMAKDIELANLKKLKNDRLVKSDHTQQEKHSTKPLDQILA
>Chafa1916S17672
MAPPPPPPKTLTSLSDTNTFESRFERDEDERPKVPYNVFSDDIPVISLAGILDESRRPEIRRKIVEACEEWGIFQVVDHGVDLDLISEMTTLSRNFFALSPEEKLRYDMSGGKKGGFIVSSHLQGEAVMDWREMVTYFSYPMEKRDYSRWPEKPEAWKEVTKEYSEKLMGLACKLLEILSEAMGLDKEALTKSCLEMDQKVVVNYYPKCPKPEVTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKSWITVQPVHGAFVVNLGDHAHYLSNGRFKNADHQAVVNSEQSRLSIATFQNPAPEATVYPLNISDGEKSVMEEAITYAEMYRRKMSGDIELAKLKKLAKEKAMGCNSHDLQKPKLDAKPIDQIFA
Sheng jun & Dong yang Teams, Yunnan agriculture University