Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Chamaecrista fasciculata
Species name
Golden cassia (Chamaecrista fasciculata) is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Chamaecrista_fasciculata.4CL.fa
Download
>Chafa1707S16992
MSMMMSVQGPSVESTQNTELASSLSSNSPKHQNSSSSDSAAAVVGKLGSRRDDGDDEEHIFKSKLADIEISNHLPLHSYCFEKLGEVAERPCLIVGSTGRIYTYAETHLLSRKIAAGLSKKVGIRKGDVIMILLQNCPEFVLSFLAASMIGAVATTANPFYTAAEILKQCKASKAKLVITQSMYVGKLREALSEEEVVVEVMTIDEPPEEDKCMHFSVVSCADERELEEVEIKPDDPVALPFSSGTTGLPKGVILTHRSLTTSVGQQVDGDNPNLYLREEDVLLCVLPLFHIFSLNSVLLCALRAGSGVLLMPKFEIMALLELTQRHKVSVAMVVPPLVLALAKNPIVQDFDLSSIRLVLSGAAPLGKDLEQALRARLPNAVLGQGYGMTEAGPVLSMCLGFAKESFPTKSGSCGTVVRNAELKVVDPETARSLSYNQPGEICIRGQQIMKGYLNDDKATAATIDAEGWLHTGDIGYVDDNDEIFIVDRVKELIKFKGFQVPPAELEGLLVSHPSIADAAVVPQKDEVAGEVPVAFVVRSNGLDLTEEAVKDFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLENATQMP
>Chafa2317S08219
MATTTTITSTTSAISTTTESATIPNKDKDIIFRSQLPDIPIPKHLPLHSYCFQNLHQFGSRPCLINAVTGKVYTYHDVDLTARRVAAALHHKFSIRRGDVIMVLLPNSPEFVFSFLAASHLGAVTTAANPFFTAAEIAKQARASNAKVLITQSSYYDKIKDLDGIKIVFVDAAVNISGHSHFSDLLNSDERDLPEVEIDPNDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHEEDVILCVLPLFHIYSLNSVLLCGLRAKATILVMPKFEINALLGLIEKHKVTIAPVVPPIVLAISKSPDLDKYDLSSIRVLKSGGAPLGKELEDAVRAKFPKAKLGQGYGMTEAGPVLTMSLAFAKEAREVKAGACGSVVRNAEMKIVDPETSLSLPRNHPGEICIRGDQIMKGYLNDPEATKRTIDEEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLSHPHISDAAVVPMKDEAAGEVPVAFVVRSNGYTDTTEDDIKQFISKQVVFYKRISKVFFVDAIPKSPSGKILRKDLRAKLAAAA
>Chafa3429S09202
MQRSGYGRDGIYRSLRPSLVLPKNDDLSIVSFLFRDISSFPSKPALIDSDSSQTLSFSELKSLVLKVSHGFLHLGIKKNDVVLIFAPNSILFPVCFLGITAVGGVACTANPISTVSEVSKQVKDSNPKLIVTVSELWDKVKDFNLPALILPSHTQISSNSKNTTTFDEFLRLAGSPTEFPSCKVKQSDTAALLYSSGTTGESKGVILTHRNFIAASLTIGMDDELAGEKHDVYLCVLPMFHVFGLAVLLYSQLRRGNAVVSMNKFDFEMLLRAVEKYRVTRLWVVPPIILDLAKQSVVHKFDLSSLKQIGSGAAPLGKELMEDCAKNLPHVLIFQGYGMTETCGIVSMENPRVGIRHSGSVGMLASGVEAQIISVDTLKPLPPPQLGEIWVRGPNMMRGYYNNPQATRLTIDKMGWVHTGDLGYFDEDGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEIADAVVIPYPDAEAGEVPAAYVVRTPNSSLTEEDVQKFIADQVSPYKRLRRVTFINAVPKSASGKILRRLLIEKARSKI
>Chafa1712S07352
MWGPFATTSITGAHYFLTLVDDYSRAVWTYLMRDKGQTVQLLFNFFAYVKTQFGKDIKNGIVERKHRHLVQVARALLFHSGLPQIFWSEAILTATHLINKLPTKLLGWKSPVELLTGTSPSYSNLRVFGCLCFVTNLDPHKKKFDVRALQGVCVGVYLLDSKKIVSSRDVKFYESNFPFKNPSSVSQPMFSSVSQFDTLFFDTATNPYLSSTSSPNNISSPTTSIAKPFDSSSLSSIQLISHSSSSNPSSSLSPASTDVISSSDIPTTSSMPLRKSTRQKKSPQWMQEYVSCSIPSSSPYTPSAFPYMSSSGFRSDYLQFLGNLTHHKDPTSYEEAKNSSEWVAAMNKELLALEQNQTWTMEADYWKESEKDAASSAGNDMKKLQLYISASSSPSTAEAFGYGALRYKGRGLGFDLRIQICFFADVRIWALSFSSEYYMFGLYLPYHVASVWGFGFSLSTLNDDVSLLNDDATSKLFILNLREFGSRPCLIKAPTGQVFTYHGDINLTARRVAAVLHHKFAIRHGDVIMVLLPNSSEFVFSFLAASHLGAVTTAANPFFTAAEIAKQARASNAKVLITQSSYYNKVKDLNGIKIIFARSRRRVGCDSVCVAVVPYIFAEFGVAVWVEGEGDDSSDAEEEELAEEDEDLIQR
>Chafa19201S17682
MGHEEEEFIFRSKLPDIYIPTHLPLHTYCFENLSEFKHRPCLIDATTGHTFTYADVHVTARRVASGLHSLGILQGHVIMLLLQNCPQFVFAFLGASFCGAVVTTANPFYTAAEVANQATASNTKLIITQSSFLHKVRDFARDKGVRVKIVAVDGGGGGGGTGAEDITGGRGGAAVLLRDDGASEGGETHKSLVTSVGQQVDGENPNLYFKNEDVIACVLPLFHIYSLNSVLLCGLRVGAAILIIHKFEIGKLLELVEKHRVSIAPFVPPIVLAIANTPNLHRYDLSSIRIIMSGAAPMGRDLEDTLRAKLPNAILGQGYGMTEAGPVLSMCLAFAKEPFGIKSGACGIVVRNAQMKILHPQTALSLPRNQAGEICIRGNQIMKGYLNDPEATKRSIDEEGWLHTGDIGYIDEDDEVFIVDRLKELIKYKGFQVAPAELESMLISHPSISDAAVVPMKDEAAGEVPVAFVVRSNASNISEDEIKQYISKQNQQGFLCGLNSKVSIRQDFAQRSKSEGDVIMLVLRNCPEFALSFLGASFRGAAVTAANPFFTTAELAKQAAASNPKLIITQAAYVDKIIDVAKTLNDDVILMCIDDASSSQGVNVKIHDFSELSQADEAEIPAVEIQPDDVVALPYSSGTSGVPKGVMLTHKNLVTTVGQLVDGENPTQYTRNDDVLLCVLPLFHIYALNSIFLCGIRAGATIVIMNKFEMTTLLEAIERYKVTVASFVPPILLALAKSEEVERYDLSSVRMVVTGAAPVSIDLVQALRAKLPRAVLGQGYGMTEGGPLAINLAFAKKAIMETKAGACGGVIRNAEMKIVDIQTGASLPRNKSGEICIRGNQVMKGYLNDPEATKRTIDKGGWLHTGDVGYIDDDDNVFIVDRLKEIIKYKGFQVAPAELEALLIAHPYISDAAVVPMQDEAAGEIPVAFVVRSNGSKISEHDIKHYVAQQVVYYKRIHRVIFTDTIPKAASGKILRKDLKARLTGATPLFN
>Chafa10563S05973
MIDGNNNNTTTTTPVTGVQISQSDAAAILFSSGTTGKVKGVVLTHRNFIALIGGFYHLRHLVDDPTAAEDELDPMSLFTLPLFHVFGFFMLIRALSMGETLVLMQRFEFEAMLRAVEKFKITYMPVSPPLMVALAKSELVSKYDLSSLRLLGCGGGGRELQS
>Chafa8198S11857
MGTSMENDEIIYRSRHSSAVTIPDGVTLPEFVLEKSEEFAETVAFVEVGTGKRITHGEVARDTRRFSKALRSLGVRKGNVVIVVLPNVAEYAVVALGIMAAGGVFSGANPTAHASEIKKQVESADAKLIVTNGLSFEKVKGLGLPVIVLDDEPIENAMNWKKLLEAADRAGPSNLTEEPIQQNDLCAMPFSSGTTGLSKGVMLTHRNLVANLCSTLFGVEPEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFELRTFLNALITHEVNYAPIVPPIILVLVKNPIVDEFDLTKLKLQAILTAAAPLAPEVLTAFENKFPGVLVQEAYGLTEHSCITLTHLHRDFGTSKRNSVGFILPNLEVKFIDPDTGKSLPRNTHGELCVRSQCVMQGYYKQEEETAQTIDKNGWLHTGDIGFIDDEDNVFIVDRIKELIKYKGFQVAPAELEAILLSHSSVEDAAVVPLPDEEAGEIPGASVVMSVGAKENEEEIMNYVASNVANYKKVRVVHFVEAIPKSPSGKIMRRLVKDRMMQKIRSSLSTNKSNHA
MSMMMSVQGPSVESTQNTELASSLSSNSPKHQNSSSSDSAAAVVGKLGSRRDDGDDEEHIFKSKLADIEISNHLPLHSYCFEKLGEVAERPCLIVGSTGRIYTYAETHLLSRKIAAGLSKKVGIRKGDVIMILLQNCPEFVLSFLAASMIGAVATTANPFYTAAEILKQCKASKAKLVITQSMYVGKLREALSEEEVVVEVMTIDEPPEEDKCMHFSVVSCADERELEEVEIKPDDPVALPFSSGTTGLPKGVILTHRSLTTSVGQQVDGDNPNLYLREEDVLLCVLPLFHIFSLNSVLLCALRAGSGVLLMPKFEIMALLELTQRHKVSVAMVVPPLVLALAKNPIVQDFDLSSIRLVLSGAAPLGKDLEQALRARLPNAVLGQGYGMTEAGPVLSMCLGFAKESFPTKSGSCGTVVRNAELKVVDPETARSLSYNQPGEICIRGQQIMKGYLNDDKATAATIDAEGWLHTGDIGYVDDNDEIFIVDRVKELIKFKGFQVPPAELEGLLVSHPSIADAAVVPQKDEVAGEVPVAFVVRSNGLDLTEEAVKDFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLENATQMP
>Chafa2317S08219
MATTTTITSTTSAISTTTESATIPNKDKDIIFRSQLPDIPIPKHLPLHSYCFQNLHQFGSRPCLINAVTGKVYTYHDVDLTARRVAAALHHKFSIRRGDVIMVLLPNSPEFVFSFLAASHLGAVTTAANPFFTAAEIAKQARASNAKVLITQSSYYDKIKDLDGIKIVFVDAAVNISGHSHFSDLLNSDERDLPEVEIDPNDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHEEDVILCVLPLFHIYSLNSVLLCGLRAKATILVMPKFEINALLGLIEKHKVTIAPVVPPIVLAISKSPDLDKYDLSSIRVLKSGGAPLGKELEDAVRAKFPKAKLGQGYGMTEAGPVLTMSLAFAKEAREVKAGACGSVVRNAEMKIVDPETSLSLPRNHPGEICIRGDQIMKGYLNDPEATKRTIDEEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLSHPHISDAAVVPMKDEAAGEVPVAFVVRSNGYTDTTEDDIKQFISKQVVFYKRISKVFFVDAIPKSPSGKILRKDLRAKLAAAA
>Chafa3429S09202
MQRSGYGRDGIYRSLRPSLVLPKNDDLSIVSFLFRDISSFPSKPALIDSDSSQTLSFSELKSLVLKVSHGFLHLGIKKNDVVLIFAPNSILFPVCFLGITAVGGVACTANPISTVSEVSKQVKDSNPKLIVTVSELWDKVKDFNLPALILPSHTQISSNSKNTTTFDEFLRLAGSPTEFPSCKVKQSDTAALLYSSGTTGESKGVILTHRNFIAASLTIGMDDELAGEKHDVYLCVLPMFHVFGLAVLLYSQLRRGNAVVSMNKFDFEMLLRAVEKYRVTRLWVVPPIILDLAKQSVVHKFDLSSLKQIGSGAAPLGKELMEDCAKNLPHVLIFQGYGMTETCGIVSMENPRVGIRHSGSVGMLASGVEAQIISVDTLKPLPPPQLGEIWVRGPNMMRGYYNNPQATRLTIDKMGWVHTGDLGYFDEDGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEIADAVVIPYPDAEAGEVPAAYVVRTPNSSLTEEDVQKFIADQVSPYKRLRRVTFINAVPKSASGKILRRLLIEKARSKI
>Chafa1712S07352
MWGPFATTSITGAHYFLTLVDDYSRAVWTYLMRDKGQTVQLLFNFFAYVKTQFGKDIKNGIVERKHRHLVQVARALLFHSGLPQIFWSEAILTATHLINKLPTKLLGWKSPVELLTGTSPSYSNLRVFGCLCFVTNLDPHKKKFDVRALQGVCVGVYLLDSKKIVSSRDVKFYESNFPFKNPSSVSQPMFSSVSQFDTLFFDTATNPYLSSTSSPNNISSPTTSIAKPFDSSSLSSIQLISHSSSSNPSSSLSPASTDVISSSDIPTTSSMPLRKSTRQKKSPQWMQEYVSCSIPSSSPYTPSAFPYMSSSGFRSDYLQFLGNLTHHKDPTSYEEAKNSSEWVAAMNKELLALEQNQTWTMEADYWKESEKDAASSAGNDMKKLQLYISASSSPSTAEAFGYGALRYKGRGLGFDLRIQICFFADVRIWALSFSSEYYMFGLYLPYHVASVWGFGFSLSTLNDDVSLLNDDATSKLFILNLREFGSRPCLIKAPTGQVFTYHGDINLTARRVAAVLHHKFAIRHGDVIMVLLPNSSEFVFSFLAASHLGAVTTAANPFFTAAEIAKQARASNAKVLITQSSYYNKVKDLNGIKIIFARSRRRVGCDSVCVAVVPYIFAEFGVAVWVEGEGDDSSDAEEEELAEEDEDLIQR
>Chafa19201S17682
MGHEEEEFIFRSKLPDIYIPTHLPLHTYCFENLSEFKHRPCLIDATTGHTFTYADVHVTARRVASGLHSLGILQGHVIMLLLQNCPQFVFAFLGASFCGAVVTTANPFYTAAEVANQATASNTKLIITQSSFLHKVRDFARDKGVRVKIVAVDGGGGGGGTGAEDITGGRGGAAVLLRDDGASEGGETHKSLVTSVGQQVDGENPNLYFKNEDVIACVLPLFHIYSLNSVLLCGLRVGAAILIIHKFEIGKLLELVEKHRVSIAPFVPPIVLAIANTPNLHRYDLSSIRIIMSGAAPMGRDLEDTLRAKLPNAILGQGYGMTEAGPVLSMCLAFAKEPFGIKSGACGIVVRNAQMKILHPQTALSLPRNQAGEICIRGNQIMKGYLNDPEATKRSIDEEGWLHTGDIGYIDEDDEVFIVDRLKELIKYKGFQVAPAELESMLISHPSISDAAVVPMKDEAAGEVPVAFVVRSNASNISEDEIKQYISKQNQQGFLCGLNSKVSIRQDFAQRSKSEGDVIMLVLRNCPEFALSFLGASFRGAAVTAANPFFTTAELAKQAAASNPKLIITQAAYVDKIIDVAKTLNDDVILMCIDDASSSQGVNVKIHDFSELSQADEAEIPAVEIQPDDVVALPYSSGTSGVPKGVMLTHKNLVTTVGQLVDGENPTQYTRNDDVLLCVLPLFHIYALNSIFLCGIRAGATIVIMNKFEMTTLLEAIERYKVTVASFVPPILLALAKSEEVERYDLSSVRMVVTGAAPVSIDLVQALRAKLPRAVLGQGYGMTEGGPLAINLAFAKKAIMETKAGACGGVIRNAEMKIVDIQTGASLPRNKSGEICIRGNQVMKGYLNDPEATKRTIDKGGWLHTGDVGYIDDDDNVFIVDRLKEIIKYKGFQVAPAELEALLIAHPYISDAAVVPMQDEAAGEIPVAFVVRSNGSKISEHDIKHYVAQQVVYYKRIHRVIFTDTIPKAASGKILRKDLKARLTGATPLFN
>Chafa10563S05973
MIDGNNNNTTTTTPVTGVQISQSDAAAILFSSGTTGKVKGVVLTHRNFIALIGGFYHLRHLVDDPTAAEDELDPMSLFTLPLFHVFGFFMLIRALSMGETLVLMQRFEFEAMLRAVEKFKITYMPVSPPLMVALAKSELVSKYDLSSLRLLGCGGGGRELQS
>Chafa8198S11857
MGTSMENDEIIYRSRHSSAVTIPDGVTLPEFVLEKSEEFAETVAFVEVGTGKRITHGEVARDTRRFSKALRSLGVRKGNVVIVVLPNVAEYAVVALGIMAAGGVFSGANPTAHASEIKKQVESADAKLIVTNGLSFEKVKGLGLPVIVLDDEPIENAMNWKKLLEAADRAGPSNLTEEPIQQNDLCAMPFSSGTTGLSKGVMLTHRNLVANLCSTLFGVEPEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFELRTFLNALITHEVNYAPIVPPIILVLVKNPIVDEFDLTKLKLQAILTAAAPLAPEVLTAFENKFPGVLVQEAYGLTEHSCITLTHLHRDFGTSKRNSVGFILPNLEVKFIDPDTGKSLPRNTHGELCVRSQCVMQGYYKQEEETAQTIDKNGWLHTGDIGFIDDEDNVFIVDRIKELIKYKGFQVAPAELEAILLSHSSVEDAAVVPLPDEEAGEIPGASVVMSVGAKENEEEIMNYVASNVANYKKVRVVHFVEAIPKSPSGKIMRRLVKDRMMQKIRSSLSTNKSNHA
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University