Enzyme
Isolated from the plants Perilla frutescens and Gentiana triflora (clustered gentian). Acts on a range of anthocyanidin 3-O-glucosides, 3,5-di-O-glucosides and cyanidin 3-rutinoside. It did not act on delphinidin 3,3',7-tri-O-glucoside. Recombinant Perilla frutescens enzyme could utilize caffeoyl-CoA but not malonyl-CoA as alternative acyl donor.
ANAT
Enzyme
Anthocyanin acyltransferase
Enzyme name
EC:2.3.1.215
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanin-3-O-glucoside 6''-O-acyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3-O-beta-D-glucoside = CoA + an anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [RN:R10237]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [CPD:C20461]
Product
Species information
Cercis canadensis
Species name
Cercis canadensis is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Cercis_canadensis.ANAT.fa
Download
>Cerca22S01818
MSMMISVKVIEKCQVSPPPGSVPSTSLPLTFFDILWFYSHPIQRIFFYEFPYPTQSFLQTLLPTLKHSLSLTLQHFFPFTANLIYPPKSHIPYILYLDGNSIPFTIAESTADFNHLVSDSPKDVTNLHPFVPVLPSPRSLEDDTRSIPVMAIQVTVLPNSGFAICVTFNHIAGDGRAFHHFMKFWSSVCGQKGDLTCLEDSLPLPFHNRDIIPDPEGIKLIHLRVYVEFCTSKHGICRAPPIHDVFTDKVHATFVLSRDQIEGQKKWVSFKCGSDHLGMLHISTFVVTSSLIWVCLVRSEETIVYNIPNNYELYHLIFLADCSNRPEFSVPSTYFGNCLVTRIVTFKRSDLVGENAIVEAAKAIESQVRDVKSDPLKGLDILSDYRESKIWAFSWNCRLTKAGCVRDRFWVGKA
>Cerca49S03555
MNSEHIILYLRLSLTLIHLCLATPMAHEHDHQSVKIIEKCQVSPPPRSIPSTSIPLSFLDILWFYSHPIQRIFFYKFPYPTQSFLQTLIPTLKHSLSLTLQHFFPFAANLIYPPKPHIPYILYLDGNSIPFIVAESTADFNHLVSDSPKDVTDLHPFVPMLSSPGTLEDGTRSIPLMAIQVTVLPNSGFALCITFSHVAWDGRAFHHFMKFWSSLCSKNGDFTSLEASLPLPFHNRDIIPDPKGLKLIHMEYMWNSPPERMEFAGVFTDKVRATFVLSRDQIERLKKWIYVKCNNCSNHHEISVPSTYFGNCLANRIVTIKRSDLVGKNAIVEAAKAIESQVRDVQSNPLKGLDTVLSDCKELLKSGHLVVIAGSPKLGVYETDFGWGKPKKSEIVHIDFSNSVAFSDCRNKEDGIEVGVALGRIQMNNFTTILKKHLSDITHFDFKSKSIIGSVSTHQD
>Cerca109S05434
MAHHQHTVKLLEQCQISPPTASVPTTCIPLSFFDIPWLLTGPAQRIFFYEFPHPTTHFMQQILPILKHSLSLTLQLFFPFAATLKCPPQPHKPHIDYIDGDSVCFTVAESTADFTHLIGDSPEDV
>Cerca109S05438
MSSKDFTVHVLEQCRVSPPSNSTPTKSLPLTFFDIPWLFFSPSKPLFFYEFPHSISQFMTIIIPKLKHSLSLTLQHYYPFAANFISPPEPAKPHLVFTDGNSISLTIAESNGDFDHFCSYHPRDVNEFHSLVPKLTPFEKKDSPLLAIQVTIFPNAGFCMGLTYHHVVADGRTFNNFIKTWASYCRLETSSFTLKLLPFYDRSVIIDTHELEGVLLKQWRKRELAQGIVIGREVQVDLSSVVRATFIMGSAEMEHIKHWIIARCKKKNLSKPLHLSAYVLTCAFLWVCLLKTEQTENEQKQVLYSEDPSYLGFVAGGITRLPFPVPNKYLGNCVGFGRASGLRNELLGEDGIVVAAKAIANTVKQLDRSIFSGSDKWILDWDVFFGSELHVMVTGSPKVDLYGTDFGWGRPKKIEEISIDSTKAISLTESRDLEGGIEIGIALPKDKMDTFTISFTQGLKALNFMIYSNHC
>Cerca427S24273
MIFFCRFPYIALLEDSHHYIRWSPIQNLKSWVYDQYKNELASTHVSTFVVVCYLVWVCLLKSQDIMVNLSDDDELCHCVFSADCRNRYEFSIPERYFGNCIMIFLVPLKRKELVGELGLVQAVKAIGTKVK
>Cerca427S24274
MILSIVNKHSVVNSDSWIIDSGATSHVCHSLKFFTSYKHLSNHFVTLPNNSQVSVSAVGIVKLSPDLILNKVLYIPDFHVNLILVTALLKHSLLTVTSHNDSFQIQDPKRSRMIDRGDLCEGLYIYQATLGNQQVVADIGKVHVVQNSSQNSIVNTQFSVLSFNSSNCISEVHNPSHAHLWHARLVIKFLFHIQCIILQILVKFVPFLSLEDYLSLIQKICPLILLISCTVTYGALIIIPLMMVRITSSQLLMTAHGSLGFIYSSISQKLQLPLSIFFVMIKTQFGKIIKQIRTDNAKELALTTFLQKERIIHQFFTARNLFMMPSSTTAPIATVDAPVTDLAHVTVPKIAHPRRTMALTYQGVCEKPHLFFDLGCLVKSQDSIVSYSGNDNLCYYMFMADCRNCYKFLIPETYFRNCIAFCYVSLRMKELAGEFGLSNENYSNGAERWMLTSKEIRQSGNQGCLPKI
>Cerca427S24276
MEISMVYGRNTLSYSIKYCGLMIKALAHLAQIIELKSFSKHLVELHPDIVQLGDAVSFTVVESAADFNRIAADYPRDIRDLHPLTPELPSACDLEDGSRALPLMAIQVTLSPCSGFCIGVIFHHAVADGSAFHHFMKSWRPFVELEDPLRSQSHCIEELKYIWARAEFLKRVVELKSKQGMDKKPNNHICNDYVRATIILSQTQI
>Cerca572S03971
MAEASNIKVLNHFWVAPRAGSVPPTSLPLTFFDLPWLSITPVTRLFFYEHPYPTHHFIQTILPTLKNSLSLTLQQFFPFAANLICPPKPHKPHILYADGDGAVSFTVAESAADLAHLAADYPRDVRDLHPLTPELPSARELDNGSSALPLIAIQVTVFPSSGFSIGIIFNHAVADGRAFHHFMKSWASICRTGEFLEEPEPLHNRVEIEDINGLQQSFLSKWWNEGSKLAVDETTNHDIFIGKVRATITLGGAQIQNLKSWVYDQYQNELASTHVSTFVVVCSLIWVCLLNSQDIMVNCSDDDELCHCVFSADCRNRYEFTIPERYFGNCITISLVPLKRKELVGELGLVQAIKAIGAKVKELEGGALKGAEKWMMTSEEIRELGQQVMIVAASPKFGVYKTDFGLGKLKKTELVHVDRPGNISLSESRDEEGGGIEVGLALPKLQMDNFNAIIKQYLELN
MSMMISVKVIEKCQVSPPPGSVPSTSLPLTFFDILWFYSHPIQRIFFYEFPYPTQSFLQTLLPTLKHSLSLTLQHFFPFTANLIYPPKSHIPYILYLDGNSIPFTIAESTADFNHLVSDSPKDVTNLHPFVPVLPSPRSLEDDTRSIPVMAIQVTVLPNSGFAICVTFNHIAGDGRAFHHFMKFWSSVCGQKGDLTCLEDSLPLPFHNRDIIPDPEGIKLIHLRVYVEFCTSKHGICRAPPIHDVFTDKVHATFVLSRDQIEGQKKWVSFKCGSDHLGMLHISTFVVTSSLIWVCLVRSEETIVYNIPNNYELYHLIFLADCSNRPEFSVPSTYFGNCLVTRIVTFKRSDLVGENAIVEAAKAIESQVRDVKSDPLKGLDILSDYRESKIWAFSWNCRLTKAGCVRDRFWVGKA
>Cerca49S03555
MNSEHIILYLRLSLTLIHLCLATPMAHEHDHQSVKIIEKCQVSPPPRSIPSTSIPLSFLDILWFYSHPIQRIFFYKFPYPTQSFLQTLIPTLKHSLSLTLQHFFPFAANLIYPPKPHIPYILYLDGNSIPFIVAESTADFNHLVSDSPKDVTDLHPFVPMLSSPGTLEDGTRSIPLMAIQVTVLPNSGFALCITFSHVAWDGRAFHHFMKFWSSLCSKNGDFTSLEASLPLPFHNRDIIPDPKGLKLIHMEYMWNSPPERMEFAGVFTDKVRATFVLSRDQIERLKKWIYVKCNNCSNHHEISVPSTYFGNCLANRIVTIKRSDLVGKNAIVEAAKAIESQVRDVQSNPLKGLDTVLSDCKELLKSGHLVVIAGSPKLGVYETDFGWGKPKKSEIVHIDFSNSVAFSDCRNKEDGIEVGVALGRIQMNNFTTILKKHLSDITHFDFKSKSIIGSVSTHQD
>Cerca109S05434
MAHHQHTVKLLEQCQISPPTASVPTTCIPLSFFDIPWLLTGPAQRIFFYEFPHPTTHFMQQILPILKHSLSLTLQLFFPFAATLKCPPQPHKPHIDYIDGDSVCFTVAESTADFTHLIGDSPEDV
>Cerca109S05438
MSSKDFTVHVLEQCRVSPPSNSTPTKSLPLTFFDIPWLFFSPSKPLFFYEFPHSISQFMTIIIPKLKHSLSLTLQHYYPFAANFISPPEPAKPHLVFTDGNSISLTIAESNGDFDHFCSYHPRDVNEFHSLVPKLTPFEKKDSPLLAIQVTIFPNAGFCMGLTYHHVVADGRTFNNFIKTWASYCRLETSSFTLKLLPFYDRSVIIDTHELEGVLLKQWRKRELAQGIVIGREVQVDLSSVVRATFIMGSAEMEHIKHWIIARCKKKNLSKPLHLSAYVLTCAFLWVCLLKTEQTENEQKQVLYSEDPSYLGFVAGGITRLPFPVPNKYLGNCVGFGRASGLRNELLGEDGIVVAAKAIANTVKQLDRSIFSGSDKWILDWDVFFGSELHVMVTGSPKVDLYGTDFGWGRPKKIEEISIDSTKAISLTESRDLEGGIEIGIALPKDKMDTFTISFTQGLKALNFMIYSNHC
>Cerca427S24273
MIFFCRFPYIALLEDSHHYIRWSPIQNLKSWVYDQYKNELASTHVSTFVVVCYLVWVCLLKSQDIMVNLSDDDELCHCVFSADCRNRYEFSIPERYFGNCIMIFLVPLKRKELVGELGLVQAVKAIGTKVK
>Cerca427S24274
MILSIVNKHSVVNSDSWIIDSGATSHVCHSLKFFTSYKHLSNHFVTLPNNSQVSVSAVGIVKLSPDLILNKVLYIPDFHVNLILVTALLKHSLLTVTSHNDSFQIQDPKRSRMIDRGDLCEGLYIYQATLGNQQVVADIGKVHVVQNSSQNSIVNTQFSVLSFNSSNCISEVHNPSHAHLWHARLVIKFLFHIQCIILQILVKFVPFLSLEDYLSLIQKICPLILLISCTVTYGALIIIPLMMVRITSSQLLMTAHGSLGFIYSSISQKLQLPLSIFFVMIKTQFGKIIKQIRTDNAKELALTTFLQKERIIHQFFTARNLFMMPSSTTAPIATVDAPVTDLAHVTVPKIAHPRRTMALTYQGVCEKPHLFFDLGCLVKSQDSIVSYSGNDNLCYYMFMADCRNCYKFLIPETYFRNCIAFCYVSLRMKELAGEFGLSNENYSNGAERWMLTSKEIRQSGNQGCLPKI
>Cerca427S24276
MEISMVYGRNTLSYSIKYCGLMIKALAHLAQIIELKSFSKHLVELHPDIVQLGDAVSFTVVESAADFNRIAADYPRDIRDLHPLTPELPSACDLEDGSRALPLMAIQVTLSPCSGFCIGVIFHHAVADGSAFHHFMKSWRPFVELEDPLRSQSHCIEELKYIWARAEFLKRVVELKSKQGMDKKPNNHICNDYVRATIILSQTQI
>Cerca572S03971
MAEASNIKVLNHFWVAPRAGSVPPTSLPLTFFDLPWLSITPVTRLFFYEHPYPTHHFIQTILPTLKNSLSLTLQQFFPFAANLICPPKPHKPHILYADGDGAVSFTVAESAADLAHLAADYPRDVRDLHPLTPELPSARELDNGSSALPLIAIQVTVFPSSGFSIGIIFNHAVADGRAFHHFMKSWASICRTGEFLEEPEPLHNRVEIEDINGLQQSFLSKWWNEGSKLAVDETTNHDIFIGKVRATITLGGAQIQNLKSWVYDQYQNELASTHVSTFVVVCSLIWVCLLNSQDIMVNCSDDDELCHCVFSADCRNRYEFTIPERYFGNCITISLVPLKRKELVGELGLVQAIKAIGAKVKELEGGALKGAEKWMMTSEEIRELGQQVMIVAASPKFGVYKTDFGLGKLKKTELVHVDRPGNISLSESRDEEGGGIEVGLALPKLQMDNFNAIIKQYLELN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University