Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Cercis canadensis
Species name
Cercis canadensis is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Cercis_canadensis.HI4_OMT.fa
Download
>Cerca40S23856
MSSLETNSASEFFEAQAYLYKYVFSIVSSMALKCAVQLNIPEIIHNHGKPITLPELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKICKEKVEKEAYTLTPPSKLLVKGKNPCLASMLMMFINPGYIKPLNFMACWFGGEEKTVYDSAFGISFWDLLQKDPERLRSFNEAMAGDSQMINMALKDCKEVFKGLESIVDVGGGTGTTARIITEAFPSLKYIVFDLPQVVGNLSGSNNLSFVAGSMFESIPSADAVLLKWILHNWSDEDCIKILKKCKQAVSGKDNGGKLIIIDVVINEREDNNDMSEVKLAYDVLMMTTLSGKERNKKEWDKILLEAGFKSYKINPIFGYRSLIEAYP
>Cerca115S00277
MASLETNPASEFFEAQAYLYKYVFSIVSSMALKCAVQLNIPEIIHNHGKPITLPELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKICKEKEEKEAYTLTPPSKFLVKGKDPCLASMLMTFVDPGLIKPLNFMACWFGGEEKSVIDSAFGISFWDLLQKDPECLRSFNEAMAGDSQMINMALKDCKEVFMGLESIVDVGGGTGTTARIITEAIPSLKYIVFDLPQVVGNLTGSNNLSFVAGSMFESIPSADAVLLKWILHDWSDEDCIKILKKCKQAILGKGNGGKLIIIDVVINGREDNHDMSEVKLAFDVLMMTIFSGKERNEKEWDKILLEAGFKSYKINPIFGYRPFYVWCIPSLGKWMQGEEATLAETILGASYYEILHNNPEYLSAFNEAMASDSQMVNLTLSDCKTVFEGLDSIVDVGGGTETTAKILSEAFPNLKCIVLDLTQVVANLTGSKNLSYVGGDTFKSIPQADAVLLKSDEDRIKILKKCKEAISNRGNGGKVIVIDVVINEKKDNYEMTEVKLFYDIVMMTTLNGKERSEKEWDKLFFEARFSSYKITPIFGFRSLIEAYP
>Cerca145S14612
MGSIEGIVIDEANELFEAQTHLYKHTYNYLSSMSLKFGIELGIPEIIQKHGKPITLSVLVSVLQLHLTKASKEPCLSPIVLSFLQPHMLDIWHFSKS
>Cerca216S01649
MDSTHGNEASELFQAQIHLYKHTGGLISSMSLKCAIDLGIPDIIHNHGKPMTLHELVSALQIHPTKTSHFQRFLRLLVHNGFFATTNKEEEEAYVLTPPSKLLVRGKDPCLSPVVHGTLIPAWCNALLSWKNWFHGEEVQLFKSEYGISFIDFLTQNPEVWSTLNEALAADSQMMRLVVKDFKPIFESLGTIVDVGGGTGTVARIISEAFPNLKCTVLELPMVVAILPESKNLSFVGGDMFESIPPADAILLKNVLHDWDDEHCIKVLKNCRDAISNKGKEGKVIIIELVIDEKNDEHGLTKMKLYYDIMMMTLANGKERNEKEWEKLFLESGFKSYKISLTFGSKSVIEVYP
>Cerca216S18009
MSSLETNPASELFEAQAHLYKYVFSIVSSMALKCAVQLNIPEIIHNHGKPITLPELISNLEVHPTKTCYVDRLVRLLVHHGFLAETKICKEKEEKEAYTLTPPSELLVKGKDPCLASMLLMFVDPGHIKPLNFMACWFGGEEKTANDIAFGISAWDSLQKDPESLRSFNEAMAGDSQMINIALKDCKEVFKSLESIVDVGGGTGTTARIITEAFPSLKYIVFDLPQVVGNLSGSNNLSFVAGSMFESIPSADAVLLKCILHNWSDEDCIKILKKCKQAVSGKSNGGKLIIIDVVINGREDDHDMSEVKLTFDVHMMATFSGKERNEKEWDKILLEAGFKSYKINPIFGFRSLIEAYP
>Cerca216S18011
MGSAHGNEANELFQAQTHLYKHTCSLISSMSLKCAIDLGIPDIIDNHGKPMTIPELVSALQIHPTKTSHLRRFLRLLEHNGFFATTNKEGEEAYVLTPPSKLLVRGKEPCLSPVVQGMLLPAWCNALHSLKNWFHGDVTLFETSYGISLWEFLNQNHEVSSTLNETMAADSQMMRLALKDCKPVFESLGTIVDVGGGTGTVARTISEAFPNLKCIVLDLPRVVANLPESKNLSYIGGDMFESIPPADAILLKSVLHNWDDEHCIKVLKNCREAISSKGKEGKVIIIELVIDGKNDDHGLTEMKLYNDILMMTLVNGKERYEKEWEKLFLESGFRSYKINPIFGFKSIIEYAIDIGIPDIIHNHVHNGFFATTNKEEEEAYVLTPPSKLLVSGKEPCLSPLVHGTLIPAWCNSLHSWKNWFHDEVTLFKSENRISFWDHEIWSTLNEAMAVDSQMMRLALKDCKPLFQALVHLYRHIYSFLTPMSLKWAVQFGIPDIIHNHGQPITLLELVSAFQVPPSKIQKLLVCTA
>Cerca216S18013
MGSSHGSEASELVQAQTHLFKHTCGLISSMSLKCAIDLGMPDIIDNHAKPMTFPELVSALQIHPAKTSHLRRFLRLLEHNGFLATTNKEEEEAYVLTPPSKLLVRGKEPYFSPIVNGLLIPSWCNSLHSWKNWFHGEEMILFNSVYGISFWDFLTQNPDAFSVFNESMVTFSPMFRLAFKDCKPVFESLGTIVDVGGGTGHVSEIISEAFPNLRCIVLDLPHVVGNLPESKNLSYVGGDMFESIPPADAVLLANSK
>Cerca299S20586
MGSLETNPASEFFEAQAYLYKYVFNFVSSMALKCAVQLNIPEIIHNHSKPITLPELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKICKEKEEKEAYTLTPPSKLLVKGKDPCLASMLLTFVDPGLIKPLNFMTCWFGGEEKTVNDSAFGISFWDLLQKDPQCLRSFNKAMAGDSQIINMAIKDCKEVFKGLESIVDVGGGTGTTARIITEAFPSLKYIVFDLPQVVGNLLGSNDLSFVAGSMFESIPSADAVLLKCILHDWSDEDCIKILKKCKQAVSGKGNGGKLIIIDVVINGREDNHDMSEVKLAYDVLMMAIFSGKERNEKEWEKILLEAGFKSYKINPIFGYRSLIEAYP
>Cerca432S09044
MNSLEINPASEFFEAQACLYKHIFSFISSMALKCAVELNIPEIIHNHGKPITLPELISNLEVHPTKTCQVDRLVRLLVHNGILAETKIYEEKEEKEAYTLTPPSKFLVKGKEPCLTSVLMAFVDPGIMKTWNFFGSWFRGEEKTVVDSAYGVSFWDILQKDPEFMKSFNEGMASDSQMINMALKDCKAVFEGLESIVDVGGGTGNTARIISEAFPSLKCIVFDLPQVVGNLSGSNNLSFVGGSMFESIPSADAVLLKWVLHNWNDEDCIKILKECKQAISSKSKRGKLIVIDVVIYRKEDKRDMTEVKLLFDIAMMATLNGKERNEKEWDKLFFEAGFKSYKITPIFGFRSLIEAYP
>Cerca442S24627
MDSLETNLASEFFEAQAYLYKYVFSIVSSMALKCAVDLNIPEIIHNHGKPITLPELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKIYKEKEEKEAYTLTPPSKFLVKGKNPCLASMTTGFVDSGVIKPLNFMARWFGGEEKSATESAFGFGYWDLLRQNPECMESFNNGMASDSQMINVALKDCKGVFNGLDSIVDVGGGTGTTARIISEAFPSLKCIVFDLPQVVGNLSGSNNLSFVGGSMLESIPSADAVLLKWILHDWSDEDCIKILKKCKQAISGKGNGGKLIIIDVVINGREDNRDTTEVKLGLDVLMMAIFNGKERNEKEWDKLLFEAGFMSYKITPIFGLRSLIEAYP
>Cerca850S33856
MGSLETNPANEFFEAQAYLYKYVFSFVSSLALKCAVQLNIPEIIHNHGKPITLSELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKICKEKEENFWDLLKKDPECLRSFNEAMAGDSQMINMAIKDCKKVFKGLESIVDVGGGTGTTARIITEAFPSLKYIVFDLPQDPECLRSFNEAMAGDSQMINMAIKDCKKVFKGLESIVDVGGGTGTTARIITEAFPSLKYIVFDLPQVVGNLSGSNNLSFVSSSMFESIPSADAVLLKQAFLGKDNGGKLIIIDVVINGWEDNHDMSEVKLAFDVLMMSIFSGKERNQKEWDKILLEAGFKSYKINPIFGYRSLIEAYP
>Cerca1249S13325
MGSLETNPASEFFEAQAYLYKYVFSIVSSMALKCAVELNIPEIIHNHGKPITLPELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKIYKEKEEKEAYTLTPPSKFLVKGKNPCLASMTTGFVDSGLIKPLNFMARWFGGEEKSATESAFGFGFWDLLRQNPECMESFNNGMASDSQMINMALKDCKGVFNGLDSIVDVGGGTGTTARIISEAFPSLKCIVFDLPQVVGNLSGSNNLSFVGGSMLESIPSADAVLLKWILHDWSDEDCIKILKKCKQALSGKGNGGKLIIIDVVIKGREDNHDMTEVKLGLDVLMMTIFNGKERNEKEWDKLLFEAGFTSYKITPIFGLRNQSAIMASIDGEKSIFFRAKAHLYKHMHSFLTPMCLKWALKFSIPDIIHNYGRPVTLAELVSALQVPPAEANCVHRIMCLLVHNGFFATIKTNDNKEEEEEAYALTPASELLVKGTEYCLSSMI
MSSLETNSASEFFEAQAYLYKYVFSIVSSMALKCAVQLNIPEIIHNHGKPITLPELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKICKEKVEKEAYTLTPPSKLLVKGKNPCLASMLMMFINPGYIKPLNFMACWFGGEEKTVYDSAFGISFWDLLQKDPERLRSFNEAMAGDSQMINMALKDCKEVFKGLESIVDVGGGTGTTARIITEAFPSLKYIVFDLPQVVGNLSGSNNLSFVAGSMFESIPSADAVLLKWILHNWSDEDCIKILKKCKQAVSGKDNGGKLIIIDVVINEREDNNDMSEVKLAYDVLMMTTLSGKERNKKEWDKILLEAGFKSYKINPIFGYRSLIEAYP
>Cerca115S00277
MASLETNPASEFFEAQAYLYKYVFSIVSSMALKCAVQLNIPEIIHNHGKPITLPELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKICKEKEEKEAYTLTPPSKFLVKGKDPCLASMLMTFVDPGLIKPLNFMACWFGGEEKSVIDSAFGISFWDLLQKDPECLRSFNEAMAGDSQMINMALKDCKEVFMGLESIVDVGGGTGTTARIITEAIPSLKYIVFDLPQVVGNLTGSNNLSFVAGSMFESIPSADAVLLKWILHDWSDEDCIKILKKCKQAILGKGNGGKLIIIDVVINGREDNHDMSEVKLAFDVLMMTIFSGKERNEKEWDKILLEAGFKSYKINPIFGYRPFYVWCIPSLGKWMQGEEATLAETILGASYYEILHNNPEYLSAFNEAMASDSQMVNLTLSDCKTVFEGLDSIVDVGGGTETTAKILSEAFPNLKCIVLDLTQVVANLTGSKNLSYVGGDTFKSIPQADAVLLKSDEDRIKILKKCKEAISNRGNGGKVIVIDVVINEKKDNYEMTEVKLFYDIVMMTTLNGKERSEKEWDKLFFEARFSSYKITPIFGFRSLIEAYP
>Cerca145S14612
MGSIEGIVIDEANELFEAQTHLYKHTYNYLSSMSLKFGIELGIPEIIQKHGKPITLSVLVSVLQLHLTKASKEPCLSPIVLSFLQPHMLDIWHFSKS
>Cerca216S01649
MDSTHGNEASELFQAQIHLYKHTGGLISSMSLKCAIDLGIPDIIHNHGKPMTLHELVSALQIHPTKTSHFQRFLRLLVHNGFFATTNKEEEEAYVLTPPSKLLVRGKDPCLSPVVHGTLIPAWCNALLSWKNWFHGEEVQLFKSEYGISFIDFLTQNPEVWSTLNEALAADSQMMRLVVKDFKPIFESLGTIVDVGGGTGTVARIISEAFPNLKCTVLELPMVVAILPESKNLSFVGGDMFESIPPADAILLKNVLHDWDDEHCIKVLKNCRDAISNKGKEGKVIIIELVIDEKNDEHGLTKMKLYYDIMMMTLANGKERNEKEWEKLFLESGFKSYKISLTFGSKSVIEVYP
>Cerca216S18009
MSSLETNPASELFEAQAHLYKYVFSIVSSMALKCAVQLNIPEIIHNHGKPITLPELISNLEVHPTKTCYVDRLVRLLVHHGFLAETKICKEKEEKEAYTLTPPSELLVKGKDPCLASMLLMFVDPGHIKPLNFMACWFGGEEKTANDIAFGISAWDSLQKDPESLRSFNEAMAGDSQMINIALKDCKEVFKSLESIVDVGGGTGTTARIITEAFPSLKYIVFDLPQVVGNLSGSNNLSFVAGSMFESIPSADAVLLKCILHNWSDEDCIKILKKCKQAVSGKSNGGKLIIIDVVINGREDDHDMSEVKLTFDVHMMATFSGKERNEKEWDKILLEAGFKSYKINPIFGFRSLIEAYP
>Cerca216S18011
MGSAHGNEANELFQAQTHLYKHTCSLISSMSLKCAIDLGIPDIIDNHGKPMTIPELVSALQIHPTKTSHLRRFLRLLEHNGFFATTNKEGEEAYVLTPPSKLLVRGKEPCLSPVVQGMLLPAWCNALHSLKNWFHGDVTLFETSYGISLWEFLNQNHEVSSTLNETMAADSQMMRLALKDCKPVFESLGTIVDVGGGTGTVARTISEAFPNLKCIVLDLPRVVANLPESKNLSYIGGDMFESIPPADAILLKSVLHNWDDEHCIKVLKNCREAISSKGKEGKVIIIELVIDGKNDDHGLTEMKLYNDILMMTLVNGKERYEKEWEKLFLESGFRSYKINPIFGFKSIIEYAIDIGIPDIIHNHVHNGFFATTNKEEEEAYVLTPPSKLLVSGKEPCLSPLVHGTLIPAWCNSLHSWKNWFHDEVTLFKSENRISFWDHEIWSTLNEAMAVDSQMMRLALKDCKPLFQALVHLYRHIYSFLTPMSLKWAVQFGIPDIIHNHGQPITLLELVSAFQVPPSKIQKLLVCTA
>Cerca216S18013
MGSSHGSEASELVQAQTHLFKHTCGLISSMSLKCAIDLGMPDIIDNHAKPMTFPELVSALQIHPAKTSHLRRFLRLLEHNGFLATTNKEEEEAYVLTPPSKLLVRGKEPYFSPIVNGLLIPSWCNSLHSWKNWFHGEEMILFNSVYGISFWDFLTQNPDAFSVFNESMVTFSPMFRLAFKDCKPVFESLGTIVDVGGGTGHVSEIISEAFPNLRCIVLDLPHVVGNLPESKNLSYVGGDMFESIPPADAVLLANSK
>Cerca299S20586
MGSLETNPASEFFEAQAYLYKYVFNFVSSMALKCAVQLNIPEIIHNHSKPITLPELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKICKEKEEKEAYTLTPPSKLLVKGKDPCLASMLLTFVDPGLIKPLNFMTCWFGGEEKTVNDSAFGISFWDLLQKDPQCLRSFNKAMAGDSQIINMAIKDCKEVFKGLESIVDVGGGTGTTARIITEAFPSLKYIVFDLPQVVGNLLGSNDLSFVAGSMFESIPSADAVLLKCILHDWSDEDCIKILKKCKQAVSGKGNGGKLIIIDVVINGREDNHDMSEVKLAYDVLMMAIFSGKERNEKEWEKILLEAGFKSYKINPIFGYRSLIEAYP
>Cerca432S09044
MNSLEINPASEFFEAQACLYKHIFSFISSMALKCAVELNIPEIIHNHGKPITLPELISNLEVHPTKTCQVDRLVRLLVHNGILAETKIYEEKEEKEAYTLTPPSKFLVKGKEPCLTSVLMAFVDPGIMKTWNFFGSWFRGEEKTVVDSAYGVSFWDILQKDPEFMKSFNEGMASDSQMINMALKDCKAVFEGLESIVDVGGGTGNTARIISEAFPSLKCIVFDLPQVVGNLSGSNNLSFVGGSMFESIPSADAVLLKWVLHNWNDEDCIKILKECKQAISSKSKRGKLIVIDVVIYRKEDKRDMTEVKLLFDIAMMATLNGKERNEKEWDKLFFEAGFKSYKITPIFGFRSLIEAYP
>Cerca442S24627
MDSLETNLASEFFEAQAYLYKYVFSIVSSMALKCAVDLNIPEIIHNHGKPITLPELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKIYKEKEEKEAYTLTPPSKFLVKGKNPCLASMTTGFVDSGVIKPLNFMARWFGGEEKSATESAFGFGYWDLLRQNPECMESFNNGMASDSQMINVALKDCKGVFNGLDSIVDVGGGTGTTARIISEAFPSLKCIVFDLPQVVGNLSGSNNLSFVGGSMLESIPSADAVLLKWILHDWSDEDCIKILKKCKQAISGKGNGGKLIIIDVVINGREDNRDTTEVKLGLDVLMMAIFNGKERNEKEWDKLLFEAGFMSYKITPIFGLRSLIEAYP
>Cerca850S33856
MGSLETNPANEFFEAQAYLYKYVFSFVSSLALKCAVQLNIPEIIHNHGKPITLSELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKICKEKEENFWDLLKKDPECLRSFNEAMAGDSQMINMAIKDCKKVFKGLESIVDVGGGTGTTARIITEAFPSLKYIVFDLPQDPECLRSFNEAMAGDSQMINMAIKDCKKVFKGLESIVDVGGGTGTTARIITEAFPSLKYIVFDLPQVVGNLSGSNNLSFVSSSMFESIPSADAVLLKQAFLGKDNGGKLIIIDVVINGWEDNHDMSEVKLAFDVLMMSIFSGKERNQKEWDKILLEAGFKSYKINPIFGYRSLIEAYP
>Cerca1249S13325
MGSLETNPASEFFEAQAYLYKYVFSIVSSMALKCAVELNIPEIIHNHGKPITLPELISNLEVHPTKTGYVDRLVRLLVHHGFLAETKIYKEKEEKEAYTLTPPSKFLVKGKNPCLASMTTGFVDSGLIKPLNFMARWFGGEEKSATESAFGFGFWDLLRQNPECMESFNNGMASDSQMINMALKDCKGVFNGLDSIVDVGGGTGTTARIISEAFPSLKCIVFDLPQVVGNLSGSNNLSFVGGSMLESIPSADAVLLKWILHDWSDEDCIKILKKCKQALSGKGNGGKLIIIDVVIKGREDNHDMTEVKLGLDVLMMTIFNGKERNEKEWDKLLFEAGFTSYKITPIFGLRNQSAIMASIDGEKSIFFRAKAHLYKHMHSFLTPMCLKWALKFSIPDIIHNYGRPVTLAELVSALQVPPAEANCVHRIMCLLVHNGFFATIKTNDNKEEEEEAYALTPASELLVKGTEYCLSSMI
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University