Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Aegilops tauschii
Species name
Aegilops tauschii is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Aegilops_tauschii.HI4_OMT.fa
Download
>rna-XM_020307252.2
MSSHVQEDELAMSSDELLQAQLELYHHSFVYVKSMALGAATDLRIADAIHLRGGAATLSDLAAHTGIHPTKFSHLRRLMRVLTTSGIFSADVKARGDTVYKLTRVSRLLVGAGGESSRRRDLSPMVGVFVNPVAIAALFSIREWFTDENSAASSSLFEMAHGCTRWEMIAKDAGEGRVFNAGMAADSCLSMEILLKECSSVFGSLGSSLVDVGGAHGTAAAVVAKAFPHVKCTVLDLPRVVAGAPAHANLTFVPGDMFEYIPPADTVLLKWVLHDWPDEDCIKILRQCKKAIPTWDAGGKVIIMDMVVGSQGLQQETVSKEAEVLFDVFIMHIDGIQREEHEWRKIFFDAGFSDYKITPVTGIRSIIEVYP
>rna-XM_020332545.2
MALTVKSTDDHALLNAEHELWATTFSYIKSMALKSALDLRLADAIHHHGGAATLPQIAARVTVHPSKIPCLRRLMRTLTVSGVFSVVQQQDVVVPAASVSNGTCNGGVAAEPLYALTPVSRLLVGSRNLVSIITMLLSPNFVTSFLGIGAWFEHSLPDPCLFTQAHGEALWTMADRDATFDALINDAMVSDSSFIMDIAIRECGEVFQGIGSLMDVGGGLGAAAQAISGAFPSLECTVMDLDHVVAKAPAGTDVNYVVGDMFESVPPADAVFLKWVLHDWGHEDCVKILKNCKKSIPPREKGGKVIIIDIVIGAGPSHVKHRELQSMFDLYMMIVDGIERDEQEWEKIFLEAGFSGYKVIHVLGFRSIIEVYP
>rna-XM_020320754.2
MSALMQQKELAMSTDELLQAQVELYHHSFAFVKSLALKAATDLCIPDAIQHLGGAATLSDLAAETGIHPTKLSNLRRLMRVLTTSGVFSVTGDHAGAVYKLTRVSRLLVVDRAGGRTHLSPVVSAFVSPYPVTTLFSMREWFTDERAAAMSLFEVAHGCTLWEAAAMDGTAFNAAMAADSRFTMENLLLECGSVFETVRGSLVDVGGGHGAAAAAIARAFPHVKCTVLDLPHVVATAPTDDKLTFVGGDMFDNVPPADAVLLKWVLHDWQHEDCVKILRRCKEAIPARNVGGTVIIVDTVIGSAGSQQGIASKETEVLFDVFMMAADGVEREEHEWSKIFIEAGFTDYKITPTMGLRSIIELYP
>rna-XM_020308761.2
MAEEESRAKMATSSAELLQAEAELVRHSLGYLKSMALHSAVKLGIPDALHRCGGAASLPDLLSTLALPCSKRPYLSRLMKMLAVEGIFTAVAAVDVPAAGEGEGAAAAPSVRYGLNPVSRLLVSGGVSPCVLMGTSPLFLEASLRLPQWFQRDSEGEPAFAMAHGESPYAATGHDMEFNALVNEAMGSDSRYMAELVVRECGEVFTGVTSLVDVGGGNGTMATAIAKAFPHVRCSVLDLPHVVQGVVSTHESVEFAAGDMMEYVPPADAVLLKCVLHNWSDEDCVKILTKCREAIAQGAKAGAGKVIIIDAVVGSPSHSQVLEAQVLMDMQMMMLFMSKEREELNWQKIFMEAGFSHYKIQPVLGMRSIIQLYP
>rna-XM_040401540.1
MRTELNKGLSIIQHTMASHDQEEELTMSMSSDELLQAQLELYNHCFAFVKSMALAAATDLRIADAIHLRGGAATLSDLAAETGTHPTKLSHLRRLMRVLTTSGVFSADVKASGGDAVYKLTRVSRLLVGAGGEDSRRRDLSPMVGVFVNPVAVTALFSIREWFIDETSAASSLFEVAHGCTRWEMIAKDAGDDRVFNAGMVADSCLSMEILLRECSGVFGSVGSSLVDVGGAHGTAAAAVAKAFPHVKCSVLDLPLVVAGAPAVDNLTFIAGDMFEYIPPADTVLLKWILHDWPDEDCIKILRQCKKAIPTRDAGGKVIIMDMVVGSAVPQQGTVSKEAEVLFDVFMMYIDGIQREEHEWSKVFFEAGFSDYKITPVTGIRSIIEVYP
>rna-XM_020326201.2
MATPNQEDELTMSTDEFLEAQAELYHHCIAFVKSLTLKAATDLGVADAIHRRGGAATLSDLASETGIHPTKLSNLRRIMRVLTASGIFSVQHSDVVSDDDAVYKLTRVSRLLVKSSSYQLSPVVPVLVDPFAWSALLRMPEWLATDERASLFDLAHGCPLWDTTVEDGGLFKDGMAADSRVAMHVLLARCGGVFEAVRSSLVDVGGSHGTTAAAVAKAFPHIKCTVLDLPDVVAGAPANDAAVTFVAGDMFEYIPPADAILLKWILHDWKDEDCIKILRRCKEAIPSRDAGGKVIIIETVVGSGSPGAQQIFSKEAQVLMDVFMMYIDGIEREEHEWSKIFFEAGFSDYKITPTNGVLSVIEVYP
MSSHVQEDELAMSSDELLQAQLELYHHSFVYVKSMALGAATDLRIADAIHLRGGAATLSDLAAHTGIHPTKFSHLRRLMRVLTTSGIFSADVKARGDTVYKLTRVSRLLVGAGGESSRRRDLSPMVGVFVNPVAIAALFSIREWFTDENSAASSSLFEMAHGCTRWEMIAKDAGEGRVFNAGMAADSCLSMEILLKECSSVFGSLGSSLVDVGGAHGTAAAVVAKAFPHVKCTVLDLPRVVAGAPAHANLTFVPGDMFEYIPPADTVLLKWVLHDWPDEDCIKILRQCKKAIPTWDAGGKVIIMDMVVGSQGLQQETVSKEAEVLFDVFIMHIDGIQREEHEWRKIFFDAGFSDYKITPVTGIRSIIEVYP
>rna-XM_020332545.2
MALTVKSTDDHALLNAEHELWATTFSYIKSMALKSALDLRLADAIHHHGGAATLPQIAARVTVHPSKIPCLRRLMRTLTVSGVFSVVQQQDVVVPAASVSNGTCNGGVAAEPLYALTPVSRLLVGSRNLVSIITMLLSPNFVTSFLGIGAWFEHSLPDPCLFTQAHGEALWTMADRDATFDALINDAMVSDSSFIMDIAIRECGEVFQGIGSLMDVGGGLGAAAQAISGAFPSLECTVMDLDHVVAKAPAGTDVNYVVGDMFESVPPADAVFLKWVLHDWGHEDCVKILKNCKKSIPPREKGGKVIIIDIVIGAGPSHVKHRELQSMFDLYMMIVDGIERDEQEWEKIFLEAGFSGYKVIHVLGFRSIIEVYP
>rna-XM_020320754.2
MSALMQQKELAMSTDELLQAQVELYHHSFAFVKSLALKAATDLCIPDAIQHLGGAATLSDLAAETGIHPTKLSNLRRLMRVLTTSGVFSVTGDHAGAVYKLTRVSRLLVVDRAGGRTHLSPVVSAFVSPYPVTTLFSMREWFTDERAAAMSLFEVAHGCTLWEAAAMDGTAFNAAMAADSRFTMENLLLECGSVFETVRGSLVDVGGGHGAAAAAIARAFPHVKCTVLDLPHVVATAPTDDKLTFVGGDMFDNVPPADAVLLKWVLHDWQHEDCVKILRRCKEAIPARNVGGTVIIVDTVIGSAGSQQGIASKETEVLFDVFMMAADGVEREEHEWSKIFIEAGFTDYKITPTMGLRSIIELYP
>rna-XM_020308761.2
MAEEESRAKMATSSAELLQAEAELVRHSLGYLKSMALHSAVKLGIPDALHRCGGAASLPDLLSTLALPCSKRPYLSRLMKMLAVEGIFTAVAAVDVPAAGEGEGAAAAPSVRYGLNPVSRLLVSGGVSPCVLMGTSPLFLEASLRLPQWFQRDSEGEPAFAMAHGESPYAATGHDMEFNALVNEAMGSDSRYMAELVVRECGEVFTGVTSLVDVGGGNGTMATAIAKAFPHVRCSVLDLPHVVQGVVSTHESVEFAAGDMMEYVPPADAVLLKCVLHNWSDEDCVKILTKCREAIAQGAKAGAGKVIIIDAVVGSPSHSQVLEAQVLMDMQMMMLFMSKEREELNWQKIFMEAGFSHYKIQPVLGMRSIIQLYP
>rna-XM_040401540.1
MRTELNKGLSIIQHTMASHDQEEELTMSMSSDELLQAQLELYNHCFAFVKSMALAAATDLRIADAIHLRGGAATLSDLAAETGTHPTKLSHLRRLMRVLTTSGVFSADVKASGGDAVYKLTRVSRLLVGAGGEDSRRRDLSPMVGVFVNPVAVTALFSIREWFIDETSAASSLFEVAHGCTRWEMIAKDAGDDRVFNAGMVADSCLSMEILLRECSGVFGSVGSSLVDVGGAHGTAAAAVAKAFPHVKCSVLDLPLVVAGAPAVDNLTFIAGDMFEYIPPADTVLLKWILHDWPDEDCIKILRQCKKAIPTRDAGGKVIIMDMVVGSAVPQQGTVSKEAEVLFDVFMMYIDGIQREEHEWSKVFFEAGFSDYKITPVTGIRSIIEVYP
>rna-XM_020326201.2
MATPNQEDELTMSTDEFLEAQAELYHHCIAFVKSLTLKAATDLGVADAIHRRGGAATLSDLASETGIHPTKLSNLRRIMRVLTASGIFSVQHSDVVSDDDAVYKLTRVSRLLVKSSSYQLSPVVPVLVDPFAWSALLRMPEWLATDERASLFDLAHGCPLWDTTVEDGGLFKDGMAADSRVAMHVLLARCGGVFEAVRSSLVDVGGSHGTTAAAVAKAFPHIKCTVLDLPDVVAGAPANDAAVTFVAGDMFEYIPPADAILLKWILHDWKDEDCIKILRRCKEAIPSRDAGGKVIIIETVVGSGSPGAQQIFSKEAQVLMDVFMMYIDGIEREEHEWSKIFFEAGFSDYKITPTNGVLSVIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University