Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Cenchrus americanus
Species name
Bulrush millet (Cenchrus americanus) is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Cenchrus_americanus.HI4_OMT.fa
Download
>Pgl_GLEAN_10033935
MDALSTEDLIQAERVLYHHFFCYVKSMALSCAVNLGIPKAIHLRGGSATLSNLIADMGLHASRLPYLQRLMNVLTISSVFTASGSSATGSLSTEETETVYKLTTASHLLVAGDKLCGMSRMLHFLVRPLTITPFFGMHAWFKDEQTTSKSFFEMAHGCDRWEMTRKNAEDNSIFNEAMVADSQFVMEIILREAGSKIFSGLSSLVDVGGGLGAASAAITRAFPRIKCSVLDLPQVISQAPQCDGTVHFIAGDMFKFIPPADAVFLKWVLHCWSDDDCIKILRNCKAAIPAREDGGKVIIIDTVMGSGPQDTISKETRALYDLYMMYINGVERGEYDWKKIFEQAGFTNYKIVSGLGMRSVIEVYP
>Pgl_GLEAN_10025175
MGSVQEQHCTDEQALLEAQLELWHSTFAFIKSMALKSAMELRIADAIHHHGGTATIAQIAAEVQLHPSKIPPMRRLMRVLSVTGIFSTIADNSAGGDGDGGGGCAYGLTPASQLLVGSSLSLTPTLSLILNNTFVSPFLGLGTWFEHERPDVTLFEMSHGKTVWDVIGHDETMSPLFNAGMVADSRFLMDIAIKKCGYVFEGVSSLVDVAGGHGAATQAISNAFPHIDCSVLELSHVVASAPDRAGVKYIAGDMFESIPPANAVFLKWVIHDWADSECVTLLKNCKKAIPPRDAGGKVIIVDTVIGAGPSNLKHRETEVLYDLFIMIVNGIERDEQEWRKIIFEAGFSDYKITPVLGVRSIIELYP
>Pgl_GLEAN_10023364
MALTVSSTNQALLDAQLELWHTTFTHIKSMALKSALDLRIADAIDHHGGAATLPQILTRITLHPSKIPCLRRLLRVLTTTGVFGAHHPAADDGCDGREQVYALTPVSRLLVGPSNMNPLTTLLLDTTFVSPFLGLGAWLQLPVPMSLFEMKHGQAIWDFADQNQAFGDMFNEACVSDSSFIMNIVTEECVGIFEGVTSLVDVAGGLGGAAQAIAKAFPHITCSVLDLPHVIANAPASTDVKYIAGDMFESVPPANSVFLKWILHDWRDDDCVKILKNCKRAIPPRHAGGKVIILDMVVGAGSSNQMHKETQVLLDLFMMFMNNGVERDEQEWKRIIFQAGFSDYKITPILGVRSIIEVYP
>Pgl_GLEAN_10023362
MGLDGGSTNQALLDAQLELWHCTFAYIKSMALKSAVDLGIADAIHHHGGGATLPQIVARVTPHPSKTPHLRRLMRVLAATGVFSVVQPPAGNSSGGKDNEPMYTLTPLSQLLVGGSKHNLAPVTALVLHPTMLTPFFELGNWLLQRELPEPDPGIFKQAHGGEALWERASRDGAFDALVNDGMASDSRFIMDIAVEECAGVFRGIGSLVDVGGGLGAAAQAIARAFPDVKCTVLDFDHVVAKAPRGTDVRYIGGDMFESIPPADVMFFKWVLHDWGDEECVIVLKNCRKAIPPREEGGKVIIMDIVIGAGSSSSDLRHKEMQALFDIYMMVVNGKERNEQEWKKIFLEAGFSDYKITPVLGVRSIIEVYP
>Pgl_GLEAN_10002382
MALTTTTNGALLDAQLELWHTTFAYIKSMALKSALDLRIADAIHGNGGTATLPEIVSGVTPPLHPSKIPCLRRLMRVLTATGIFSVQHPDDGNVSGGELIYGLTPASQLLVGTASLAPFMSLVLHGIYVSPFLGLGTWLQRQEHVDHRSLFEMTHGRSAWELNDHNPIFGALFDEGMVCDSSFIMDIVIKECGDVFRGLSSLIDVAGGLGGAAQSICKAFPHVACSVLDLPHVVAKTASSSTMASANVKYVAGDMFKSIPPANATFLKWVLHDWSDADCVKILKNCRKAVPPRDAGGKVIVLDMVVGAGSSNNKHKETQVLFDLFIMFVNGAERDEQEWRKIIFEAGFSDYKIIPVLGVRSIIEIYP
>Pgl_GLEAN_10002383
MALTTPTQSSANQVLLDAQVELWNSTFAYIKSMALKSALDLRIPDAIHSHGGVATLPQIVATVQLHPSKIPCLRRLMRVLGATGLLSAQSNPSGSGGEPAYALTPVSRLLVGSQNNLAPITAMILHPSFVSPFLELGAWFQKEEQPESCVFEHTHGQTLWEHAGRDAAFDALVNDGMVSDSHFIMDIAFKECADAFQGISSLVDVGGGLGAAAQAIAKAFPGVKCSVLDLDHVVAKAPRDIGVQYIAGDMFESVPPADAVFFKWVLHDWGDEDCVRILKNCRKAIPPREEGGKVIIIDIVVEQGSSSNSKQRETQALFDLYIMLVNGIERDEQEWKNIFIKAGFSDYKISPVLGARSIIEVYP
MDALSTEDLIQAERVLYHHFFCYVKSMALSCAVNLGIPKAIHLRGGSATLSNLIADMGLHASRLPYLQRLMNVLTISSVFTASGSSATGSLSTEETETVYKLTTASHLLVAGDKLCGMSRMLHFLVRPLTITPFFGMHAWFKDEQTTSKSFFEMAHGCDRWEMTRKNAEDNSIFNEAMVADSQFVMEIILREAGSKIFSGLSSLVDVGGGLGAASAAITRAFPRIKCSVLDLPQVISQAPQCDGTVHFIAGDMFKFIPPADAVFLKWVLHCWSDDDCIKILRNCKAAIPAREDGGKVIIIDTVMGSGPQDTISKETRALYDLYMMYINGVERGEYDWKKIFEQAGFTNYKIVSGLGMRSVIEVYP
>Pgl_GLEAN_10025175
MGSVQEQHCTDEQALLEAQLELWHSTFAFIKSMALKSAMELRIADAIHHHGGTATIAQIAAEVQLHPSKIPPMRRLMRVLSVTGIFSTIADNSAGGDGDGGGGCAYGLTPASQLLVGSSLSLTPTLSLILNNTFVSPFLGLGTWFEHERPDVTLFEMSHGKTVWDVIGHDETMSPLFNAGMVADSRFLMDIAIKKCGYVFEGVSSLVDVAGGHGAATQAISNAFPHIDCSVLELSHVVASAPDRAGVKYIAGDMFESIPPANAVFLKWVIHDWADSECVTLLKNCKKAIPPRDAGGKVIIVDTVIGAGPSNLKHRETEVLYDLFIMIVNGIERDEQEWRKIIFEAGFSDYKITPVLGVRSIIELYP
>Pgl_GLEAN_10023364
MALTVSSTNQALLDAQLELWHTTFTHIKSMALKSALDLRIADAIDHHGGAATLPQILTRITLHPSKIPCLRRLLRVLTTTGVFGAHHPAADDGCDGREQVYALTPVSRLLVGPSNMNPLTTLLLDTTFVSPFLGLGAWLQLPVPMSLFEMKHGQAIWDFADQNQAFGDMFNEACVSDSSFIMNIVTEECVGIFEGVTSLVDVAGGLGGAAQAIAKAFPHITCSVLDLPHVIANAPASTDVKYIAGDMFESVPPANSVFLKWILHDWRDDDCVKILKNCKRAIPPRHAGGKVIILDMVVGAGSSNQMHKETQVLLDLFMMFMNNGVERDEQEWKRIIFQAGFSDYKITPILGVRSIIEVYP
>Pgl_GLEAN_10023362
MGLDGGSTNQALLDAQLELWHCTFAYIKSMALKSAVDLGIADAIHHHGGGATLPQIVARVTPHPSKTPHLRRLMRVLAATGVFSVVQPPAGNSSGGKDNEPMYTLTPLSQLLVGGSKHNLAPVTALVLHPTMLTPFFELGNWLLQRELPEPDPGIFKQAHGGEALWERASRDGAFDALVNDGMASDSRFIMDIAVEECAGVFRGIGSLVDVGGGLGAAAQAIARAFPDVKCTVLDFDHVVAKAPRGTDVRYIGGDMFESIPPADVMFFKWVLHDWGDEECVIVLKNCRKAIPPREEGGKVIIMDIVIGAGSSSSDLRHKEMQALFDIYMMVVNGKERNEQEWKKIFLEAGFSDYKITPVLGVRSIIEVYP
>Pgl_GLEAN_10002382
MALTTTTNGALLDAQLELWHTTFAYIKSMALKSALDLRIADAIHGNGGTATLPEIVSGVTPPLHPSKIPCLRRLMRVLTATGIFSVQHPDDGNVSGGELIYGLTPASQLLVGTASLAPFMSLVLHGIYVSPFLGLGTWLQRQEHVDHRSLFEMTHGRSAWELNDHNPIFGALFDEGMVCDSSFIMDIVIKECGDVFRGLSSLIDVAGGLGGAAQSICKAFPHVACSVLDLPHVVAKTASSSTMASANVKYVAGDMFKSIPPANATFLKWVLHDWSDADCVKILKNCRKAVPPRDAGGKVIVLDMVVGAGSSNNKHKETQVLFDLFIMFVNGAERDEQEWRKIIFEAGFSDYKIIPVLGVRSIIEIYP
>Pgl_GLEAN_10002383
MALTTPTQSSANQVLLDAQVELWNSTFAYIKSMALKSALDLRIPDAIHSHGGVATLPQIVATVQLHPSKIPCLRRLMRVLGATGLLSAQSNPSGSGGEPAYALTPVSRLLVGSQNNLAPITAMILHPSFVSPFLELGAWFQKEEQPESCVFEHTHGQTLWEHAGRDAAFDALVNDGMVSDSHFIMDIAFKECADAFQGISSLVDVGGGLGAAAQAIAKAFPGVKCSVLDLDHVVAKAPRDIGVQYIAGDMFESVPPADAVFFKWVLHDWGDEDCVRILKNCRKAIPPREEGGKVIIIDIVVEQGSSSNSKQRETQALFDLYIMLVNGIERDEQEWKNIFIKAGFSDYKISPVLGARSIIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University