Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Catharanthus roseu
Species name
Species info
Gene sequence info
Catharanthus_roseu.HI4_OMT.fa
Download
>CRO_T033205
MEVQSVEFCKAQAHIWNQVFSFTTCASLKCAVQLGIADAIDDHGKPMSLFYLTKALQINPSKAPYIQRLMGILVNSGFFSQDKLTYYSLTPSSRLLLKKEALNLRAAVEIMLDPAELKAWSSLSDWFKDEKCRTAFEMVHGKNFWDFYAEESIHGEIFNRAMSTDSQLISKLLVTEYKFLFEGLTSLVDIGGGTGTIAKAIAKSFPKLKCTVFDLPHVVANLESKENLEFVGGDMFERIPNANAILLKIVLHNWNDEDCMKILKKCKEAILGKEKSGKVIIIDIVMDSKKEDYESLQAQISMDLQMMVLLDAKERREKEWAILFQKSGFSGYKIFSMFDYRSIIEVYP
>CRO_T019314
MDVQSDEFRKAHTYIWSQAFTFTGCASLKCAVELGIADAIHNHGKPMSLSDLTNTLEINPYKAQHLHRLMRFLANAGIFSEEAGTENVYSLTPLSRLLLKNARLNSRAFVLMMFDPLQMKAWNALSEWFKNDDQTAFETAHNGKSYWDFAVEEPRRLELFSEAMAADSLIVSEVLINKYKFFFEGLTSLVDVGGGTGELARTIAESFPTIKCSVLDLPEVVANVEKTENLEIIGGDMFENIPAANAIFLKMVLHDWNNENCVKILKNCKKAIPEKEKGGKVIILDTVVLGEVDDSESVKTQLCMDMDMMVLLGTNERSEKEWAAIIQEAGFSSYKIFPLLDIRSLIEVYP
>CRO_T008408
MVGALSSSSGSNESSSHELLLKAHAHVWNMIYNFINSMSIKCAIDLNIPDIIHNHGNPITLSELTQSLKINSSKAPYVYRLMRILTHSGFFTKSKIGRNETEEVDAYALTPSSNLLLKDNPSNINPLLSLALQPTRIRAWLNLSDWFRNDEDLTRNPFANTHDGRDIWKMSGQEQGINNLFNEAMANDAWFISSLVVENFEIFKGLTSLVDVGGGTC
>CRO_T017919
MDLETAEIRKAQAQYSRHVFSFVSTSCLKCAIQLEIPEAIHNHGKPMTLSDLTNSLPINPSKAPYIHRLMRILVAAGYFSEEPKNVYSLTSLSRILVKNQPLNLREFVLSANEIAEVEGWNALSEWFQNDVATAFQTAHGKTYWEYLSQDKYGKNFDQLMATDSLLISKLLIPDYNYLFEGLISLVDVGGGTGTLAGAVAKAFPNLKCTVFEQPHVIADLEAKGNLEFVGGDMFEKIPSANAILLKSVLHDWKDEDSVKILKNCKKAIPEKEKGGKVIVIDIVLMDSKKHDNPLVKSQISGDMDMMVSMGAKERTEEEWAALFKEAGFSGYKIFPMLDFRSPIEVYP
>CRO_T018243
KAQAHIWNQVFSFTTCASLKCAVQLGIADAIDDHGKPMSLFYLTKALQINPSKAPYIQRLMGILVNSGFFSQDKLTYYSLTPSSRLLLKKEALNLRAAVEIMLDPAELKAWSSLSDWFKDEKCRTAFEMVHGKNFWDFYAEESIHGEIFNRAMSTDSQLISKLLVTEYKFLFEGLTSLVDIGGGTGTIAKAIAKSFPKLKCTVFDLPHVVANLESKENLEFVGGDMFERIPNANAILLKIVLHNWNDEDCMKILKKCKEAILGKEKSGKVIIIDIVMDSKKEDYESLQAQISMDLQMMVLLDAKERREKEWAILFQKSGFSGYKIFSMFDYRSIIEVYP
>CRO_T012860
MEVQSVEFRKAQAHIWNQVFSFTTCASLKCAVQLGIADAIDDHGKPMSLFYLTKALQINPSKAPYIQRLMGILVNSGFFSQDKLTYYSLTPSSRLLLKKEALNLRAAVEIMLDPAELKAWSSLSDWFKDEKCRTAFEMVHGKNFWDFYAEESIHGEIFNRAMSTDSQLISKLLVTEYKFLFEGLTSLVDIGGGTGTIAKAIAKSFPKLKCTVFDLPHVVANLESKENLEFVGGDMFERIPNANAILLKIVLHNWNDEDCMKILKKCKEAILGKEKSGKVIIIDIVMDSKKEDYESLQAQISMDLQMMVLLDAKERREKEWAILFQKSGFSGYKIFSMFDYRSIIEVYP
>CRO_T012656
MELQSSEIRNAQAHFFTQVFSFTSMSSLKCAVQLGIPDAIHSHGKPMALSDLTNSLPINPSKAPYIYRLMRILVAAGYFSEEEKNVYSLTPFTRLLLKNDPLNSISMVLGVNQIAELKAWNAMSEWFQNEDLTAFETAHGKNFWDFGAEDKYGKNFDGVMAADSILVSKMLIPEFNYLFEGLDSLVDVGGGTGTIAKAIAKSFPDLKCTVFDLPHVVANLESTENLEFVGGDMFEKIPSANAILLKWILHDWKDEECVKVLKMCRKAIPEKEKGGKVILIETVLMDSKKHENEEAVKAQISSDIDMMVFFTAKERTEEEWATLFREAGFSGYKIFPMIDFRSPIEVYP
>CRO_T004356
MDVQSEEFREAQAQIWSQSCSFITSASLKCAVKLGIPDIIDNHGKPITLSELTNALVPPVHPSKAPFIYRLMRVLAKNGFCSEEQLDGETEPLYSLTLSSRILLKKEPLNLRGIVLTMADPVQLKAWESLSDWYQNEDDSSTAFEIAHGKNFWDYSSEHMEHAEFFNEAMASDSQLISKLLIGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLESKENVEFVAGDMFEKIPSANAIFLKWILHDWNDEDCVKILKSCKKAIPAKGGKVIVIDMVMYSDKKDDHLVKTQTSMDMAMLVNFAAKERCEKEWAFLFKEAGFSDYKIYPKLDFTRSLIEVYP
>CRO_T022617
MEVQSAEFRKAQVHIWSQSFSFITCASLKCALQLGIADAIENHGKPMALSDLTNTLQINPSKSQYLPRLMRLLVNSGFFSEQQLGDAGGGENVYGLTPSMRLLLKNEPLNLRGIVLPMLDPLQIKAWNSLGNWFKNEDGTAFETAHGKSYWDCVTEDPKDVEIFTETMASDSLLVAKFVITEHKSLFEGLDSIIDVGGSTGTFARAIAESFPSLKCTVLDLPETVANIEPTNNLEFVGGNMFEKIPPAHVVFLKMVLHDWNDEDCVKILKNCRKAIPEKEKGGKVILIETVVQGQMHDSESVKTQIAMDMDMLVSFGTKERTEKEWEILFKEAGFSSYKIFPIVDIRSLIEKIEMDLQTAEFREAQAKIWSQAFSFANCAALKCAVKLGIADAIDNHDKKALTLSELTEELSIKPSKSPFLQRLMRQLVNAGFFTEAKQLRDDNKDGRTTTAYALTPVSRLLLKNEQWNLRGIVLTMLDPAELKAWSVLNDWFKNDDPTAFQTAHEKNYWDYTAENTQHCQIFEDAMANDSVLVSKLLVTEYKFLFEGLTSLIDLGGSTGTIAKALAKSFPNLKCTVFDLPHVVANLESTKNLEFVGGDMFEKLPPSNAILLKWILHDWNDEDCVKILKNCKKAIQEKGNGGKVIIIDTVVYSQKNEKELVDLQISMDMAMVINFAAKERTEEEWEHLIREAGFSGHKIFPMYDFRSIIEVYP
MEVQSVEFCKAQAHIWNQVFSFTTCASLKCAVQLGIADAIDDHGKPMSLFYLTKALQINPSKAPYIQRLMGILVNSGFFSQDKLTYYSLTPSSRLLLKKEALNLRAAVEIMLDPAELKAWSSLSDWFKDEKCRTAFEMVHGKNFWDFYAEESIHGEIFNRAMSTDSQLISKLLVTEYKFLFEGLTSLVDIGGGTGTIAKAIAKSFPKLKCTVFDLPHVVANLESKENLEFVGGDMFERIPNANAILLKIVLHNWNDEDCMKILKKCKEAILGKEKSGKVIIIDIVMDSKKEDYESLQAQISMDLQMMVLLDAKERREKEWAILFQKSGFSGYKIFSMFDYRSIIEVYP
>CRO_T019314
MDVQSDEFRKAHTYIWSQAFTFTGCASLKCAVELGIADAIHNHGKPMSLSDLTNTLEINPYKAQHLHRLMRFLANAGIFSEEAGTENVYSLTPLSRLLLKNARLNSRAFVLMMFDPLQMKAWNALSEWFKNDDQTAFETAHNGKSYWDFAVEEPRRLELFSEAMAADSLIVSEVLINKYKFFFEGLTSLVDVGGGTGELARTIAESFPTIKCSVLDLPEVVANVEKTENLEIIGGDMFENIPAANAIFLKMVLHDWNNENCVKILKNCKKAIPEKEKGGKVIILDTVVLGEVDDSESVKTQLCMDMDMMVLLGTNERSEKEWAAIIQEAGFSSYKIFPLLDIRSLIEVYP
>CRO_T008408
MVGALSSSSGSNESSSHELLLKAHAHVWNMIYNFINSMSIKCAIDLNIPDIIHNHGNPITLSELTQSLKINSSKAPYVYRLMRILTHSGFFTKSKIGRNETEEVDAYALTPSSNLLLKDNPSNINPLLSLALQPTRIRAWLNLSDWFRNDEDLTRNPFANTHDGRDIWKMSGQEQGINNLFNEAMANDAWFISSLVVENFEIFKGLTSLVDVGGGTC
>CRO_T017919
MDLETAEIRKAQAQYSRHVFSFVSTSCLKCAIQLEIPEAIHNHGKPMTLSDLTNSLPINPSKAPYIHRLMRILVAAGYFSEEPKNVYSLTSLSRILVKNQPLNLREFVLSANEIAEVEGWNALSEWFQNDVATAFQTAHGKTYWEYLSQDKYGKNFDQLMATDSLLISKLLIPDYNYLFEGLISLVDVGGGTGTLAGAVAKAFPNLKCTVFEQPHVIADLEAKGNLEFVGGDMFEKIPSANAILLKSVLHDWKDEDSVKILKNCKKAIPEKEKGGKVIVIDIVLMDSKKHDNPLVKSQISGDMDMMVSMGAKERTEEEWAALFKEAGFSGYKIFPMLDFRSPIEVYP
>CRO_T018243
KAQAHIWNQVFSFTTCASLKCAVQLGIADAIDDHGKPMSLFYLTKALQINPSKAPYIQRLMGILVNSGFFSQDKLTYYSLTPSSRLLLKKEALNLRAAVEIMLDPAELKAWSSLSDWFKDEKCRTAFEMVHGKNFWDFYAEESIHGEIFNRAMSTDSQLISKLLVTEYKFLFEGLTSLVDIGGGTGTIAKAIAKSFPKLKCTVFDLPHVVANLESKENLEFVGGDMFERIPNANAILLKIVLHNWNDEDCMKILKKCKEAILGKEKSGKVIIIDIVMDSKKEDYESLQAQISMDLQMMVLLDAKERREKEWAILFQKSGFSGYKIFSMFDYRSIIEVYP
>CRO_T012860
MEVQSVEFRKAQAHIWNQVFSFTTCASLKCAVQLGIADAIDDHGKPMSLFYLTKALQINPSKAPYIQRLMGILVNSGFFSQDKLTYYSLTPSSRLLLKKEALNLRAAVEIMLDPAELKAWSSLSDWFKDEKCRTAFEMVHGKNFWDFYAEESIHGEIFNRAMSTDSQLISKLLVTEYKFLFEGLTSLVDIGGGTGTIAKAIAKSFPKLKCTVFDLPHVVANLESKENLEFVGGDMFERIPNANAILLKIVLHNWNDEDCMKILKKCKEAILGKEKSGKVIIIDIVMDSKKEDYESLQAQISMDLQMMVLLDAKERREKEWAILFQKSGFSGYKIFSMFDYRSIIEVYP
>CRO_T012656
MELQSSEIRNAQAHFFTQVFSFTSMSSLKCAVQLGIPDAIHSHGKPMALSDLTNSLPINPSKAPYIYRLMRILVAAGYFSEEEKNVYSLTPFTRLLLKNDPLNSISMVLGVNQIAELKAWNAMSEWFQNEDLTAFETAHGKNFWDFGAEDKYGKNFDGVMAADSILVSKMLIPEFNYLFEGLDSLVDVGGGTGTIAKAIAKSFPDLKCTVFDLPHVVANLESTENLEFVGGDMFEKIPSANAILLKWILHDWKDEECVKVLKMCRKAIPEKEKGGKVILIETVLMDSKKHENEEAVKAQISSDIDMMVFFTAKERTEEEWATLFREAGFSGYKIFPMIDFRSPIEVYP
>CRO_T004356
MDVQSEEFREAQAQIWSQSCSFITSASLKCAVKLGIPDIIDNHGKPITLSELTNALVPPVHPSKAPFIYRLMRVLAKNGFCSEEQLDGETEPLYSLTLSSRILLKKEPLNLRGIVLTMADPVQLKAWESLSDWYQNEDDSSTAFEIAHGKNFWDYSSEHMEHAEFFNEAMASDSQLISKLLIGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLESKENVEFVAGDMFEKIPSANAIFLKWILHDWNDEDCVKILKSCKKAIPAKGGKVIVIDMVMYSDKKDDHLVKTQTSMDMAMLVNFAAKERCEKEWAFLFKEAGFSDYKIYPKLDFTRSLIEVYP
>CRO_T022617
MEVQSAEFRKAQVHIWSQSFSFITCASLKCALQLGIADAIENHGKPMALSDLTNTLQINPSKSQYLPRLMRLLVNSGFFSEQQLGDAGGGENVYGLTPSMRLLLKNEPLNLRGIVLPMLDPLQIKAWNSLGNWFKNEDGTAFETAHGKSYWDCVTEDPKDVEIFTETMASDSLLVAKFVITEHKSLFEGLDSIIDVGGSTGTFARAIAESFPSLKCTVLDLPETVANIEPTNNLEFVGGNMFEKIPPAHVVFLKMVLHDWNDEDCVKILKNCRKAIPEKEKGGKVILIETVVQGQMHDSESVKTQIAMDMDMLVSFGTKERTEKEWEILFKEAGFSSYKIFPIVDIRSLIEKIEMDLQTAEFREAQAKIWSQAFSFANCAALKCAVKLGIADAIDNHDKKALTLSELTEELSIKPSKSPFLQRLMRQLVNAGFFTEAKQLRDDNKDGRTTTAYALTPVSRLLLKNEQWNLRGIVLTMLDPAELKAWSVLNDWFKNDDPTAFQTAHEKNYWDYTAENTQHCQIFEDAMANDSVLVSKLLVTEYKFLFEGLTSLIDLGGSTGTIAKALAKSFPNLKCTVFDLPHVVANLESTKNLEFVGGDMFEKLPPSNAILLKWILHDWNDEDCVKILKNCKKAIQEKGNGGKVIIIDTVVYSQKNEKELVDLQISMDMAMVINFAAKERTEEEWEHLIREAGFSGHKIFPMYDFRSIIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University