Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Casuarina glauca
Species name
Swamp oak (Casuarina glauca) is a species of eudicot in the family Casuarinaceae.
Species info
Gene sequence info
Casuarina_glauca.HI4_OMT.fa
Download
>Casgl1495S08969
MDLGHSQGVCEFFQVQSHLYKHIFSYIDSMSVNCANQLGIPDIIHNNGQPITLPQLVSKLHVHPKKKSCLHRLMRLLVHFGFFTKTKVLENQQEEEEACALTPTSRLLLKDNVTSMSPFALAAIHPVVVSPFFLLGDWLRGDELTPFEKAHGMGLWNYCEENPEYNYTFNQAMASHSQLMRLVVMDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPCLKCTVLDLPHVVADLPESTNLKYSGGDMFQSIPYADAILLKACIFIIFASCGRYKEAITSKDKEGKVIIIDVVIDEENDEDDIT
>Casgl164S03801
MDLVHGQEASELLQAQTDLYKHIYSYIDSMALKCAVQLGIPDIIHNHGEPITLHDLVSTLQIDPIKATFLHSLMRLLVHCGFFATRVHKNQEEEAYDLTPLSRILLKDNVASFSPLVLMVLDPALVNTWHVLEQWFRGDKVTPFESAQGMGFWDYCNQNPEFNNTFNDAMASDSRMMKLVIRDWKPIFEGLDTLVDVGGGTGESARIILEAFPHLRCVVFDLPHVVANLPESVNLRYVAGDMFQPIPSADAVLLKVWNISLTHIHFYRLSLL
>Casgl3726S05139
MDLAPTQGMSELLQVQSHLYKHIFSYVDSFSLNCAIQLGIPDIIHNNGQPITLPQLITKLHVHPRKTGYVHRLMRLLVHSGFFSKMKVHENQQEEEEAYALTPSSKLLLKDSVTSLSPFALAMVHPVMLSPWHGLGDWLRGDELNPFEKAHGMDFWNYCNHNPEFNCTFNEAMASDSQLMCLVVKDYKPIFEGLDSLVDVGGGTGTVARLISEAFPHMKCTVLDLPHVIADLPESINLKYVGGDMFQSIPSSDAILIKVCNIFHCLQTSNFCFK
>Casgl151S24207
MDPIHGQGASELFEAQSHLYRYLFSFIGSMSLKCAIQLGIPDIIHNHGEPITLHDLVSALKIHPTKAGFVHRLMRLLVHCGFFAKVRLNKDQEEEAYDLTPSSRILLKDNITCLSPFVLAMLDPALVTPWQVLGHWFRGDKVTPFESAHGMGLWDFVSSSASEEISNQFEFDCQHSSLVSKPT
>Casgl151S00452
MSSCPFTLPPSTGYESDGTSHDKNVRAVNPSRRTVFKSKVMDLIHDQGASELLQAQSDLYKHIYSYVDSMALKCAVQLGIPDIIHNHGEPISLHDLVSALQIDPTKATFIHRLMRLLVHCGFFATTRIHKNQEEAYDLTPLSRILLKDNVANFSPLVLTALDPALLTPWHVLEHWFRGDKVTPFESAHGMGFWDYCNQKPEFNDLFSDAMASDSAMMKLIIKDWKQIFEGLDTLVDVGGGTGTCARIISEAFPHLKCVVFDLPHVVANLLGNINLRYVGGDMFQSIPSADAILLK
>Casgl151S09332
MRLLVHSGFFSKTKVNENQQEDEEAYALTPSSRPFLKDKVTSLSPFALAMVHPVMISPCHGLGDWLRKDELNPFEKAHGIDFWNYYNQNPVFNYTFNEGMASDSQLICLVVKDYKPIFEGLDSLIDMGGGTGTVARIIFEAFPQINCMVFDLQHVVTDLSESTNLKYVLFQSIPSVDAIQLKWILHDWSDEECVNILRKCKGALTSKG
>Casgl151S09336
MGLWDYCNQNPEYNYTFNQAMASDSQLMRLVVRDYKPNFEALDSLVDVGGVTGAVPKIISEAFPYLKCTVLDLPHVVAGMPANSMSLNCAIQLGIPDIIHNNGQPITLPQLISKLHVHPKKTSCVGRLMRLLVHSGFFTKTKVHESQQEEEEAYALTPSSRLLLKDNVTSLSPFALGMIHPIMVSPGHVLGDWLRGDEVTPFEKVHGMDFWSYCNQNAEFNYSFNEAMASDSQLMRLVVKDYKPIFEGLDSLVDVGGGTGTVARIISEAFPHMKCTVLDIPHVVANLPESTNLKFVGGDMFQYIPSADAILVKVCIIFLSLKDPFLFFLFLFRS
>Casgl1822S10709
MDLDHSPEVCELFQAQSHLYRHIFSYIDSMSVNCAIQLGIPDIIHSNDQPITLPQLVSKLHVHPKKESCVHRLMRLLVHFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSLSPFALAWIYPVMVSPFFLLGDWLQGDELTPFEKAHGMGLWNYCKQNPEYNYTFNQAMVSDSQFMRMVVTDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPYLKCTVPDLPHVVAELPESANLKYSGGDMFQSIPYADAILLKLGIPDIIHSNDQPITLPQLVSKLHVHPKKESCVHRLMRLLVHFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSLSPFALAWIYPVMVSPFFLLGDWLQGDELTPFEKAHGMGLWNYCKQNPEYNYTFNQAMVSDSQFMRMVVTDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPYLKCTVPDLPHVVAELPESANLKYSGGDMFQSIPYADAILLKVCNIFLSFKDIFLLL
>Casgl1822S10708
MDLGHSQGVCESFQVQSHLYKHIFSYIDSMSVNCAIQLGIPDIIHNNGQPITLPKLVSKLHVHPKKKSCLHRLMRLLVYFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSMSPFALLAIHPVVVSPFFLLADWLRGDELTPFEKAHGMGLWNYCKQNPEYNYTFNQAMATDSQLMRLVVRDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPYLKCTVLDLPHVVADLPESVNLKYSGGDMFQSIPYADAILLKEILKGHYK
>Casgl322S15671
MDLGHSQGVCELFQVQSHLYKHIFSYIDSMSVNCAIQLGIPDIIHNNGQPITLPQLVSKLHVRPKKKSCVHRLMRLLVHFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSMSPFALAAIHPVVVSPFFLLGDWLRGDELTPFEKAHGMGLWNYCKQNPEYNYTFNQAMASDSQLMRLVVRDYKPIFEGLDSLDDVGGGTGAVAKIISEAFPDLKCTVLDLPHVVADLPESANLKYSGGDMFQSIPYADAILLKVCIFIIFASCGRYKEAITSKDKEGKLLLQKRDKKEWEKLLLKMKKTMKDITKAKLLLSALMMVVVIGKDRDNKE
>Casgl322S15672
MDLAHSPGVCELFQVQSQLYKHIFSDIDSMSLKCAIQLGIPGIIHNNAQPITLPQLVSKLRVQPKKKSCVHRLMPSSLKQKSLKISKKKSYALTPTSRLLLKDNVTSMSPFALAAIHPVVVSPFFLLGDWLRGDELTPFKKAHGMGLWNYCKQNPEYNYTFNQAMASDSQLMRLVVRDYKPIFEGLDSLDDVGGGTGAVAKIISEAFPDLKCTVLDLPHVVADLPESANLKYSGGDMFQSIPYADAILLKWILHDWSDAECVNIPKKSKEAITSKDKGGKVIIIDMVIDKENDEDDITKAKFLFDALMMVVVTGKERDKKEWEKILLEAGFSHYKIMPLFGMKSVIERDRKEWEKLFLEASFSHYKIMASFGIKFVIEEILRGHYK
>Casgl322S15677
MMVVVTIKEREKKEWEKLFLEASFSHYKTMPSFGMKSSIEIVRSKDKEGKVIIIEVVIDEESDCDDII
>Casgl322S15669
MDLGHSQGVCQLFQVQSHLYKHIFSYIDSMSVNCAIQLGIPDTIHNNGQPITLPQLVSKLHVHPKKKSCLLRLMRLLVHFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSMSPFALAAIPPVVGDWLRGDELTPFEKAHGMGLWNYCKQNPEYNYTFNQAMACDSQILRLVVMDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPYLKCTGLDLPHVVADLPESANLKYSGGDMFQSIPCADAILPKVCNIFLSLKFLFDALMMVVVTGKERDKKEWEKILLETGFSHYKIMPLFGMQSVIEVIIIDVVLAEENDEDDIT
>Casgl322S15673
MMVVVTRKERERKKWEKLLLEASFSHYKTMASFGMKCSIQIITSKDVEGKVITINMAINEENDGHDIT
>Casgl322S15674
MDLGHSQGVCELFQVQSHLYKHIFSYIDSMSVNCAIQLGIPDIIHNNGQPISLPQLVSKLHVHPKKKSCLHRLMRLLVHFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSLSPYALAMIHPVMVSPFFLLGDWLRGDELTPFEKSHGMDLWNYCKQNPEYNYTFNQAMTSDSQFMRLVVRDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPYLKCTVLDLPHVVADLPESANLKYSGGDMFQSIPYADAILLKFFSYNVLNWLQPLQMIYEWQCINTGKAHIFIIFASCGKYKEAITSKHKERKVIIINVVIDEENDGDDII
>Casgl322S15676
MMVVVTRKERERKEWEKLFLEESFSHYKTMASFGMKSSIQIITSKDKEGKVFIINMAIDEENDEHDIT
MDLGHSQGVCEFFQVQSHLYKHIFSYIDSMSVNCANQLGIPDIIHNNGQPITLPQLVSKLHVHPKKKSCLHRLMRLLVHFGFFTKTKVLENQQEEEEACALTPTSRLLLKDNVTSMSPFALAAIHPVVVSPFFLLGDWLRGDELTPFEKAHGMGLWNYCEENPEYNYTFNQAMASHSQLMRLVVMDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPCLKCTVLDLPHVVADLPESTNLKYSGGDMFQSIPYADAILLKACIFIIFASCGRYKEAITSKDKEGKVIIIDVVIDEENDEDDIT
>Casgl164S03801
MDLVHGQEASELLQAQTDLYKHIYSYIDSMALKCAVQLGIPDIIHNHGEPITLHDLVSTLQIDPIKATFLHSLMRLLVHCGFFATRVHKNQEEEAYDLTPLSRILLKDNVASFSPLVLMVLDPALVNTWHVLEQWFRGDKVTPFESAQGMGFWDYCNQNPEFNNTFNDAMASDSRMMKLVIRDWKPIFEGLDTLVDVGGGTGESARIILEAFPHLRCVVFDLPHVVANLPESVNLRYVAGDMFQPIPSADAVLLKVWNISLTHIHFYRLSLL
>Casgl3726S05139
MDLAPTQGMSELLQVQSHLYKHIFSYVDSFSLNCAIQLGIPDIIHNNGQPITLPQLITKLHVHPRKTGYVHRLMRLLVHSGFFSKMKVHENQQEEEEAYALTPSSKLLLKDSVTSLSPFALAMVHPVMLSPWHGLGDWLRGDELNPFEKAHGMDFWNYCNHNPEFNCTFNEAMASDSQLMCLVVKDYKPIFEGLDSLVDVGGGTGTVARLISEAFPHMKCTVLDLPHVIADLPESINLKYVGGDMFQSIPSSDAILIKVCNIFHCLQTSNFCFK
>Casgl151S24207
MDPIHGQGASELFEAQSHLYRYLFSFIGSMSLKCAIQLGIPDIIHNHGEPITLHDLVSALKIHPTKAGFVHRLMRLLVHCGFFAKVRLNKDQEEEAYDLTPSSRILLKDNITCLSPFVLAMLDPALVTPWQVLGHWFRGDKVTPFESAHGMGLWDFVSSSASEEISNQFEFDCQHSSLVSKPT
>Casgl151S00452
MSSCPFTLPPSTGYESDGTSHDKNVRAVNPSRRTVFKSKVMDLIHDQGASELLQAQSDLYKHIYSYVDSMALKCAVQLGIPDIIHNHGEPISLHDLVSALQIDPTKATFIHRLMRLLVHCGFFATTRIHKNQEEAYDLTPLSRILLKDNVANFSPLVLTALDPALLTPWHVLEHWFRGDKVTPFESAHGMGFWDYCNQKPEFNDLFSDAMASDSAMMKLIIKDWKQIFEGLDTLVDVGGGTGTCARIISEAFPHLKCVVFDLPHVVANLLGNINLRYVGGDMFQSIPSADAILLK
>Casgl151S09332
MRLLVHSGFFSKTKVNENQQEDEEAYALTPSSRPFLKDKVTSLSPFALAMVHPVMISPCHGLGDWLRKDELNPFEKAHGIDFWNYYNQNPVFNYTFNEGMASDSQLICLVVKDYKPIFEGLDSLIDMGGGTGTVARIIFEAFPQINCMVFDLQHVVTDLSESTNLKYVLFQSIPSVDAIQLKWILHDWSDEECVNILRKCKGALTSKG
>Casgl151S09336
MGLWDYCNQNPEYNYTFNQAMASDSQLMRLVVRDYKPNFEALDSLVDVGGVTGAVPKIISEAFPYLKCTVLDLPHVVAGMPANSMSLNCAIQLGIPDIIHNNGQPITLPQLISKLHVHPKKTSCVGRLMRLLVHSGFFTKTKVHESQQEEEEAYALTPSSRLLLKDNVTSLSPFALGMIHPIMVSPGHVLGDWLRGDEVTPFEKVHGMDFWSYCNQNAEFNYSFNEAMASDSQLMRLVVKDYKPIFEGLDSLVDVGGGTGTVARIISEAFPHMKCTVLDIPHVVANLPESTNLKFVGGDMFQYIPSADAILVKVCIIFLSLKDPFLFFLFLFRS
>Casgl1822S10709
MDLDHSPEVCELFQAQSHLYRHIFSYIDSMSVNCAIQLGIPDIIHSNDQPITLPQLVSKLHVHPKKESCVHRLMRLLVHFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSLSPFALAWIYPVMVSPFFLLGDWLQGDELTPFEKAHGMGLWNYCKQNPEYNYTFNQAMVSDSQFMRMVVTDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPYLKCTVPDLPHVVAELPESANLKYSGGDMFQSIPYADAILLKLGIPDIIHSNDQPITLPQLVSKLHVHPKKESCVHRLMRLLVHFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSLSPFALAWIYPVMVSPFFLLGDWLQGDELTPFEKAHGMGLWNYCKQNPEYNYTFNQAMVSDSQFMRMVVTDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPYLKCTVPDLPHVVAELPESANLKYSGGDMFQSIPYADAILLKVCNIFLSFKDIFLLL
>Casgl1822S10708
MDLGHSQGVCESFQVQSHLYKHIFSYIDSMSVNCAIQLGIPDIIHNNGQPITLPKLVSKLHVHPKKKSCLHRLMRLLVYFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSMSPFALLAIHPVVVSPFFLLADWLRGDELTPFEKAHGMGLWNYCKQNPEYNYTFNQAMATDSQLMRLVVRDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPYLKCTVLDLPHVVADLPESVNLKYSGGDMFQSIPYADAILLKEILKGHYK
>Casgl322S15671
MDLGHSQGVCELFQVQSHLYKHIFSYIDSMSVNCAIQLGIPDIIHNNGQPITLPQLVSKLHVRPKKKSCVHRLMRLLVHFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSMSPFALAAIHPVVVSPFFLLGDWLRGDELTPFEKAHGMGLWNYCKQNPEYNYTFNQAMASDSQLMRLVVRDYKPIFEGLDSLDDVGGGTGAVAKIISEAFPDLKCTVLDLPHVVADLPESANLKYSGGDMFQSIPYADAILLKVCIFIIFASCGRYKEAITSKDKEGKLLLQKRDKKEWEKLLLKMKKTMKDITKAKLLLSALMMVVVIGKDRDNKE
>Casgl322S15672
MDLAHSPGVCELFQVQSQLYKHIFSDIDSMSLKCAIQLGIPGIIHNNAQPITLPQLVSKLRVQPKKKSCVHRLMPSSLKQKSLKISKKKSYALTPTSRLLLKDNVTSMSPFALAAIHPVVVSPFFLLGDWLRGDELTPFKKAHGMGLWNYCKQNPEYNYTFNQAMASDSQLMRLVVRDYKPIFEGLDSLDDVGGGTGAVAKIISEAFPDLKCTVLDLPHVVADLPESANLKYSGGDMFQSIPYADAILLKWILHDWSDAECVNIPKKSKEAITSKDKGGKVIIIDMVIDKENDEDDITKAKFLFDALMMVVVTGKERDKKEWEKILLEAGFSHYKIMPLFGMKSVIERDRKEWEKLFLEASFSHYKIMASFGIKFVIEEILRGHYK
>Casgl322S15677
MMVVVTIKEREKKEWEKLFLEASFSHYKTMPSFGMKSSIEIVRSKDKEGKVIIIEVVIDEESDCDDII
>Casgl322S15669
MDLGHSQGVCQLFQVQSHLYKHIFSYIDSMSVNCAIQLGIPDTIHNNGQPITLPQLVSKLHVHPKKKSCLLRLMRLLVHFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSMSPFALAAIPPVVGDWLRGDELTPFEKAHGMGLWNYCKQNPEYNYTFNQAMACDSQILRLVVMDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPYLKCTGLDLPHVVADLPESANLKYSGGDMFQSIPCADAILPKVCNIFLSLKFLFDALMMVVVTGKERDKKEWEKILLETGFSHYKIMPLFGMQSVIEVIIIDVVLAEENDEDDIT
>Casgl322S15673
MMVVVTRKERERKKWEKLLLEASFSHYKTMASFGMKCSIQIITSKDVEGKVITINMAINEENDGHDIT
>Casgl322S15674
MDLGHSQGVCELFQVQSHLYKHIFSYIDSMSVNCAIQLGIPDIIHNNGQPISLPQLVSKLHVHPKKKSCLHRLMRLLVHFGFFTKTKVLENQQEEEEAYALTPTSRLLLKDNVTSLSPYALAMIHPVMVSPFFLLGDWLRGDELTPFEKSHGMDLWNYCKQNPEYNYTFNQAMTSDSQFMRLVVRDYKPIFEGLDSLVDVGGGTGAVAKIISEAFPYLKCTVLDLPHVVADLPESANLKYSGGDMFQSIPYADAILLKFFSYNVLNWLQPLQMIYEWQCINTGKAHIFIIFASCGKYKEAITSKHKERKVIIINVVIDEENDGDDII
>Casgl322S15676
MMVVVTRKERERKEWEKLFLEESFSHYKTMASFGMKSSIQIITSKDKEGKVFIINMAIDEENDEHDIT
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University